Expression of mRNA for vasoactive intestinal peptide in rat small intestine

Transplantation of small intestine is a neural model that permits studies of expression of the neuropeptide, vasoactive intestinal peptide, following extrinsic denervation, transection of intrinsic neural pathways, and an ischemic interval. Tissue levels of vasoactive intestinal peptide were examine...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 1996-01, Vol.116 (1), p.31-37
Main Authors: Stadelmann, A.M., Telford, G.L., Appel, D.A., Walgenbach-Telford, S., Hopp, K., Meier, D.A., Koch, T.R.
Format: Article
Language:English
Subjects:
Animals
Denervation
Gene expression
Gene Expression Regulation
Intestine, Small - innervation
Intestine, Small - metabolism
Intestine, Small - transplantation
Ischemia - genetics
Ischemia - metabolism
Radioimmunoassay
Rat
Rats
Rats, Inbred Lew
RNA, Messenger - genetics
RNA, Messenger - metabolism
Small intestine
Transplantation, Isogeneic
Up-Regulation
Vasoactive intestinal peptide
Vasoactive Intestinal Peptide - genetics
Vasoactive Intestinal Peptide - metabolism
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Summary:Transplantation of small intestine is a neural model that permits studies of expression of the neuropeptide, vasoactive intestinal peptide, following extrinsic denervation, transection of intrinsic neural pathways, and an ischemic interval. Tissue levels of vasoactive intestinal peptide were examined at 3 months in ileum from a sham operation, in ileum after resection of proximal small intestine, in ileum after resection of proximal small intestine and extrinsic denervation, in ileum after resection of proximal small intestine and 30 min of ischemia, and in ileum obtained 3 months after ileal isografting in Lewis-to-Lewis combinations. Vasoactive intestinal peptide levels were increased in transplanted rat ileum, resection controls, denervation controls, and ischemic controls compared to sham-operated ileum (pANOVA < 0.01). The increased levels of this peptide were highest in denervation controls and lowest in ischemic controls. Northern blot analysis using rat vasoactive intestinal peptide cDNA identified a single 1.7-kb transcript in normal and transplanted rat ileum. The density of vasoactive intestinal peptide transcripts was increased in transplanted ileum (8450 ± 540) compared to normal ileum (5790 ± 620) ( P < 0.01), and the ratio of this transcript to glyceraldehyde-3-phosphate dehydrogenase density units was also increased in transplanted ileum (0.81 ± 0.08) compared to normal ileum (0.40 ± 0.07; P < 0.01). Enhanced transcriptional regulation was the likely mechanism for increased tissue vasoactive intestinal peptide. The increased tissue levels appeared to be a response to extrinsic denervation and transection of intrinsic neural pathways, while an ischemic interval appeared to decrease tissue levels of the peptide.
ISSN:0303-7207
1872-8057
DOI:10.1016/0303-7207(95)03693-8