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Dopamine secretion by PC12 cells microencapsulated in a hydroxyethyl methacrylate-methyl methacrylate copolymer
A rat pheochromocytoma cell line (PC12) was encapsulated in a water-insoluble hydroxyethyl methacrylate-methyl methacrylate copolymer by interfacial precipitation from a polyethylene glycol 200 solution into phosphate-buffered saline. The resulting capsules (660 ± 44 μm in diameter; 84 ± 27 μm wall...
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| Published in: | Biomaterials 1996-02, Vol.17 (3), p.267-275 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c357t-acd6232ed06cc49dc2166e2c52b4b4212e526f91ac9452bf7c77238d760c02c73 |
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| cites | cdi_FETCH-LOGICAL-c357t-acd6232ed06cc49dc2166e2c52b4b4212e526f91ac9452bf7c77238d760c02c73 |
| container_end_page | 275 |
| container_issue | 3 |
| container_start_page | 267 |
| container_title | Biomaterials |
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| creator | Roberts, Todd De Boni, Umberto Sefton, Michael V. |
| description | A rat pheochromocytoma cell line (PC12) was encapsulated in a water-insoluble hydroxyethyl methacrylate-methyl methacrylate copolymer by interfacial precipitation from a polyethylene glycol 200 solution into phosphate-buffered saline. The resulting capsules (660 ± 44 μm in diameter; 84 ± 27 μm wall thickness) contained viable PC12 cells in a spheroidal arrangement, much like tumour spheroids, the latter grown on surfaces unsuitable for cell attachment. In these spheroids, the viable cells formed a band approximately 100 μm thick, surrounding an inner core of necrotic cells. A similar arrangement was seen 14, 28 and 42 days after encapsulation, with capsules maintained in an
in vitro tissue culture environment; the annular ring was roughly constant in size, although the packing density appeared to increase over the 6 week observation period. During the first 4 weeks, when measurements were made the encapsulated cells converted a tetrazolium dye (MTT) into an insoluble formazan product, in a time-after-encapsulation-dependent manner. This indicated that PC12 cells retained viability despite encapsulation and an ability to increase (at least in part) their metabolic capacity, presumably by a combination of proliferation and altered cellular activity. The encapsulated PC12 cells also secreted dopamine when incubated in a high potassium release medium but not in a low potassium, conventional tissue culture medium (RPMI 1640). Consistent with the MTT results, the amount of dopamine released was also dependent on the time after encapsulation, as well as the cell density at the time of encapsulation. |
| doi_str_mv | 10.1016/0142-9612(96)85564-5 |
| format | article |
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in vitro tissue culture environment; the annular ring was roughly constant in size, although the packing density appeared to increase over the 6 week observation period. During the first 4 weeks, when measurements were made the encapsulated cells converted a tetrazolium dye (MTT) into an insoluble formazan product, in a time-after-encapsulation-dependent manner. This indicated that PC12 cells retained viability despite encapsulation and an ability to increase (at least in part) their metabolic capacity, presumably by a combination of proliferation and altered cellular activity. The encapsulated PC12 cells also secreted dopamine when incubated in a high potassium release medium but not in a low potassium, conventional tissue culture medium (RPMI 1640). Consistent with the MTT results, the amount of dopamine released was also dependent on the time after encapsulation, as well as the cell density at the time of encapsulation.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/0142-9612(96)85564-5</identifier><identifier>PMID: 8745323</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Biocompatible Materials ; Calcium - metabolism ; Capsules ; Cell Nucleus - ultrastructure ; Cell Survival ; Chromatin - ultrastructure ; Coloring Agents ; dopamine ; Dopamine - metabolism ; HEMA ; Kinetics ; Methylmethacrylates ; Microencapsulation ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Mitochondria - ultrastructure ; Necrosis ; PC12 ; PC12 Cells ; Polyhydroxyethyl Methacrylate ; Rats ; Tetrazolium Salts ; Thiazoles ; Vacuoles - ultrastructure</subject><ispartof>Biomaterials, 1996-02, Vol.17 (3), p.267-275</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-acd6232ed06cc49dc2166e2c52b4b4212e526f91ac9452bf7c77238d760c02c73</citedby><cites>FETCH-LOGICAL-c357t-acd6232ed06cc49dc2166e2c52b4b4212e526f91ac9452bf7c77238d760c02c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8745323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roberts, Todd</creatorcontrib><creatorcontrib>De Boni, Umberto</creatorcontrib><creatorcontrib>Sefton, Michael V.</creatorcontrib><title>Dopamine secretion by PC12 cells microencapsulated in a hydroxyethyl methacrylate-methyl methacrylate copolymer</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>A rat pheochromocytoma cell line (PC12) was encapsulated in a water-insoluble hydroxyethyl methacrylate-methyl methacrylate copolymer by interfacial precipitation from a polyethylene glycol 200 solution into phosphate-buffered saline. The resulting capsules (660 ± 44 μm in diameter; 84 ± 27 μm wall thickness) contained viable PC12 cells in a spheroidal arrangement, much like tumour spheroids, the latter grown on surfaces unsuitable for cell attachment. In these spheroids, the viable cells formed a band approximately 100 μm thick, surrounding an inner core of necrotic cells. A similar arrangement was seen 14, 28 and 42 days after encapsulation, with capsules maintained in an
in vitro tissue culture environment; the annular ring was roughly constant in size, although the packing density appeared to increase over the 6 week observation period. During the first 4 weeks, when measurements were made the encapsulated cells converted a tetrazolium dye (MTT) into an insoluble formazan product, in a time-after-encapsulation-dependent manner. This indicated that PC12 cells retained viability despite encapsulation and an ability to increase (at least in part) their metabolic capacity, presumably by a combination of proliferation and altered cellular activity. The encapsulated PC12 cells also secreted dopamine when incubated in a high potassium release medium but not in a low potassium, conventional tissue culture medium (RPMI 1640). Consistent with the MTT results, the amount of dopamine released was also dependent on the time after encapsulation, as well as the cell density at the time of encapsulation.</description><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>Calcium - metabolism</subject><subject>Capsules</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cell Survival</subject><subject>Chromatin - ultrastructure</subject><subject>Coloring Agents</subject><subject>dopamine</subject><subject>Dopamine - metabolism</subject><subject>HEMA</subject><subject>Kinetics</subject><subject>Methylmethacrylates</subject><subject>Microencapsulation</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Scanning</subject><subject>Mitochondria - ultrastructure</subject><subject>Necrosis</subject><subject>PC12</subject><subject>PC12 Cells</subject><subject>Polyhydroxyethyl Methacrylate</subject><subject>Rats</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><subject>Vacuoles - ultrastructure</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNp9kE1v1DAQhq0KVJaWf1AknxAc0tqOP5ILElq-KlWCAz1b3vGs1iiJg50g8u_rsKu9IHHxyPO-M6P3IeSGs1vOuL5jXIqq1Vy8bfW7RiktK3VBNrwxTaVapp6RzdnygrzM-ScrfybFJblsjFS1qDckfoyj68OANCMknEIc6G6h37dcUMCuy7QPkCIO4MY8d25CT8NAHT0sPsU_C06HpaN9KQ7SsupV_2-PQhxjt_SYrsnzvesyvjrVK_L4-dOP7dfq4duX--2HhwpqZabKgdeiFuiZBpCtB8G1RgFK7OROCi5QCb1vuYNWlt7egDGibrzRDJgAU1-RN8e9Y4q_ZsyT7UNeA7kB45ytaYSSjNXFKI_GkjLnhHs7ptC7tFjO7MrZrhDtCrE89i9nq8rY69P-edejPw-dwBb9_VHHEvJ3wGQzhEIRfUgIk_Ux_P_AE6bVjnE</recordid><startdate>19960201</startdate><enddate>19960201</enddate><creator>Roberts, Todd</creator><creator>De Boni, Umberto</creator><creator>Sefton, Michael V.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960201</creationdate><title>Dopamine secretion by PC12 cells microencapsulated in a hydroxyethyl methacrylate-methyl methacrylate copolymer</title><author>Roberts, Todd ; De Boni, Umberto ; Sefton, Michael V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-acd6232ed06cc49dc2166e2c52b4b4212e526f91ac9452bf7c77238d760c02c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biocompatible Materials</topic><topic>Calcium - metabolism</topic><topic>Capsules</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cell Survival</topic><topic>Chromatin - ultrastructure</topic><topic>Coloring Agents</topic><topic>dopamine</topic><topic>Dopamine - metabolism</topic><topic>HEMA</topic><topic>Kinetics</topic><topic>Methylmethacrylates</topic><topic>Microencapsulation</topic><topic>Microscopy, Electron</topic><topic>Microscopy, Electron, Scanning</topic><topic>Mitochondria - ultrastructure</topic><topic>Necrosis</topic><topic>PC12</topic><topic>PC12 Cells</topic><topic>Polyhydroxyethyl Methacrylate</topic><topic>Rats</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><topic>Vacuoles - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roberts, Todd</creatorcontrib><creatorcontrib>De Boni, Umberto</creatorcontrib><creatorcontrib>Sefton, Michael V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roberts, Todd</au><au>De Boni, Umberto</au><au>Sefton, Michael V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dopamine secretion by PC12 cells microencapsulated in a hydroxyethyl methacrylate-methyl methacrylate copolymer</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>1996-02-01</date><risdate>1996</risdate><volume>17</volume><issue>3</issue><spage>267</spage><epage>275</epage><pages>267-275</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>A rat pheochromocytoma cell line (PC12) was encapsulated in a water-insoluble hydroxyethyl methacrylate-methyl methacrylate copolymer by interfacial precipitation from a polyethylene glycol 200 solution into phosphate-buffered saline. The resulting capsules (660 ± 44 μm in diameter; 84 ± 27 μm wall thickness) contained viable PC12 cells in a spheroidal arrangement, much like tumour spheroids, the latter grown on surfaces unsuitable for cell attachment. In these spheroids, the viable cells formed a band approximately 100 μm thick, surrounding an inner core of necrotic cells. A similar arrangement was seen 14, 28 and 42 days after encapsulation, with capsules maintained in an
in vitro tissue culture environment; the annular ring was roughly constant in size, although the packing density appeared to increase over the 6 week observation period. During the first 4 weeks, when measurements were made the encapsulated cells converted a tetrazolium dye (MTT) into an insoluble formazan product, in a time-after-encapsulation-dependent manner. This indicated that PC12 cells retained viability despite encapsulation and an ability to increase (at least in part) their metabolic capacity, presumably by a combination of proliferation and altered cellular activity. The encapsulated PC12 cells also secreted dopamine when incubated in a high potassium release medium but not in a low potassium, conventional tissue culture medium (RPMI 1640). Consistent with the MTT results, the amount of dopamine released was also dependent on the time after encapsulation, as well as the cell density at the time of encapsulation.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>8745323</pmid><doi>10.1016/0142-9612(96)85564-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0142-9612 |
| ispartof | Biomaterials, 1996-02, Vol.17 (3), p.267-275 |
| issn | 0142-9612 1878-5905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78254003 |
| source | ScienceDirect Journals |
| subjects | Animals Biocompatible Materials Calcium - metabolism Capsules Cell Nucleus - ultrastructure Cell Survival Chromatin - ultrastructure Coloring Agents dopamine Dopamine - metabolism HEMA Kinetics Methylmethacrylates Microencapsulation Microscopy, Electron Microscopy, Electron, Scanning Mitochondria - ultrastructure Necrosis PC12 PC12 Cells Polyhydroxyethyl Methacrylate Rats Tetrazolium Salts Thiazoles Vacuoles - ultrastructure |
| title | Dopamine secretion by PC12 cells microencapsulated in a hydroxyethyl methacrylate-methyl methacrylate copolymer |
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