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Biochemical Assay of Collagen and Elastin in the Normal and Varicose Vein Wall
Alterations of the connective tissue in the varicose vein wall have been noted by several investigators; however, the cause of the vein dilatation has still not been established. The aim of this study was to find a biochemical explanation to the development of varices by evaluating sensitive biochem...
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| Published in: | The Journal of surgical research 1996-01, Vol.60 (1), p.245-248 |
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| container_end_page | 248 |
| container_issue | 1 |
| container_start_page | 245 |
| container_title | The Journal of surgical research |
| container_volume | 60 |
| creator | Venturi, Marco Bonavina, Luigi Annoni, Federico Colombo, Laura Butera, Caterina Peracchia, Alberto Mussini, Emilio |
| description | Alterations of the connective tissue in the varicose vein wall have been noted by several investigators; however, the cause of the vein dilatation has still not been established. The aim of this study was to find a biochemical explanation to the development of varices by evaluating sensitive biochemical markers of collagen and elastin in the varicose vein wall. 4-L-Hydroxyproline (HYP), as a marker of collagen content, and desmosine (DES) and isodesmosine (IDES), as markers of elastin, were measured in 47 macroscopically dilated and 32 nondilated segments of 20 varicose saphenous veins collected from 20 patients with varices. The same measurements were made in 24 fragments of normal saphenous veins collected from 14 patients in whom the vein was removed to be used for graft procedures. HYP (collagen) and DES and IDES (elastin) were determined with a colorimetric method and HPLC, respectively. ANOVA test was used to compare mean values (±SD). HYP and collagen content were similar in varicose and normal veins. There was a significant reduction of both DES and IDES in dilated segments of varicose veins (P< 0.05 vs normal veins and nondilated segments); the ratio of elastin to collagen was lower in varicose than normal veins (P< 0.05), and this reduction was most significant in the dilated segments (P< 0.01 vs normal veins). These results suggest that dilatation of the varicose vein wall may be related to some defect of elastin metabolism. Further studies on the metabolic activity of vein muscle cells are required. |
| doi_str_mv | 10.1006/jsre.1996.0038 |
| format | article |
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The aim of this study was to find a biochemical explanation to the development of varices by evaluating sensitive biochemical markers of collagen and elastin in the varicose vein wall. 4-L-Hydroxyproline (HYP), as a marker of collagen content, and desmosine (DES) and isodesmosine (IDES), as markers of elastin, were measured in 47 macroscopically dilated and 32 nondilated segments of 20 varicose saphenous veins collected from 20 patients with varices. The same measurements were made in 24 fragments of normal saphenous veins collected from 14 patients in whom the vein was removed to be used for graft procedures. HYP (collagen) and DES and IDES (elastin) were determined with a colorimetric method and HPLC, respectively. ANOVA test was used to compare mean values (±SD). HYP and collagen content were similar in varicose and normal veins. There was a significant reduction of both DES and IDES in dilated segments of varicose veins (P< 0.05 vs normal veins and nondilated segments); the ratio of elastin to collagen was lower in varicose than normal veins (P< 0.05), and this reduction was most significant in the dilated segments (P< 0.01 vs normal veins). These results suggest that dilatation of the varicose vein wall may be related to some defect of elastin metabolism. Further studies on the metabolic activity of vein muscle cells are required.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1006/jsre.1996.0038</identifier><identifier>PMID: 8592422</identifier><identifier>CODEN: JSGRA2</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Aged ; Biochemistry - methods ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Collagen - metabolism ; Desmosine - metabolism ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Elastin - metabolism ; Female ; Humans ; Hydroxyproline - metabolism ; Isodesmosine - metabolism ; Male ; Medical sciences ; Middle Aged ; Muscle, Smooth, Vascular - diagnostic imaging ; Muscle, Smooth, Vascular - metabolism ; Reference Values ; Ultrasonography, Doppler, Color ; Varicose Veins - diagnostic imaging ; Varicose Veins - metabolism ; Veins - diagnostic imaging ; Veins - metabolism</subject><ispartof>The Journal of surgical research, 1996-01, Vol.60 (1), p.245-248</ispartof><rights>1996 Academic Press</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-85e3f1b7ee3bf35abf39cc483eebbd76ff1d49caf9454ed6ec4d39118bd323093</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,4043,27956,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3030817$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8592422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Venturi, Marco</creatorcontrib><creatorcontrib>Bonavina, Luigi</creatorcontrib><creatorcontrib>Annoni, Federico</creatorcontrib><creatorcontrib>Colombo, Laura</creatorcontrib><creatorcontrib>Butera, Caterina</creatorcontrib><creatorcontrib>Peracchia, Alberto</creatorcontrib><creatorcontrib>Mussini, Emilio</creatorcontrib><title>Biochemical Assay of Collagen and Elastin in the Normal and Varicose Vein Wall</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Alterations of the connective tissue in the varicose vein wall have been noted by several investigators; however, the cause of the vein dilatation has still not been established. The aim of this study was to find a biochemical explanation to the development of varices by evaluating sensitive biochemical markers of collagen and elastin in the varicose vein wall. 4-L-Hydroxyproline (HYP), as a marker of collagen content, and desmosine (DES) and isodesmosine (IDES), as markers of elastin, were measured in 47 macroscopically dilated and 32 nondilated segments of 20 varicose saphenous veins collected from 20 patients with varices. The same measurements were made in 24 fragments of normal saphenous veins collected from 14 patients in whom the vein was removed to be used for graft procedures. HYP (collagen) and DES and IDES (elastin) were determined with a colorimetric method and HPLC, respectively. ANOVA test was used to compare mean values (±SD). HYP and collagen content were similar in varicose and normal veins. There was a significant reduction of both DES and IDES in dilated segments of varicose veins (P< 0.05 vs normal veins and nondilated segments); the ratio of elastin to collagen was lower in varicose than normal veins (P< 0.05), and this reduction was most significant in the dilated segments (P< 0.01 vs normal veins). These results suggest that dilatation of the varicose vein wall may be related to some defect of elastin metabolism. Further studies on the metabolic activity of vein muscle cells are required.</description><subject>Adult</subject><subject>Aged</subject><subject>Biochemistry - methods</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology. Vascular system</subject><subject>Collagen - metabolism</subject><subject>Desmosine - metabolism</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</subject><subject>Elastin - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Hydroxyproline - metabolism</subject><subject>Isodesmosine - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Muscle, Smooth, Vascular - diagnostic imaging</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Reference Values</subject><subject>Ultrasonography, Doppler, Color</subject><subject>Varicose Veins - diagnostic imaging</subject><subject>Varicose Veins - metabolism</subject><subject>Veins - diagnostic imaging</subject><subject>Veins - metabolism</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNp1kM9LwzAUx4MoOqdXb0IP4q0zadI2Oc4xf4DoRecxpMmLRtpmJp2w_96UDW9CSHh8P3nv8UHoguAZwbi6-YoBZkSIaoYx5QdoQrAoc17V9BBNMC6KnHHMTtBpjF841aKmx-iYl6JgRTFBz7fO60_onFZtNo9RbTNvs4VvW_UBfaZ6ky1bFQfXZ-kMn5A9-9AldkxWKjjtI2QrSOG7atszdGRVG-F8_07R293ydfGQP73cPy7mT7lmlA05L4Fa0tQAtLG0VOkSWjNOAZrG1JW1xDChlRWsZGAq0MxQQQhvDC0oFnSKrnd918F_byAOsnNRQ9q6B7-Jsq5FRQtcJ3C2A3XwMamych1cp8JWEixHgXIUKEeBchSYPlzuO2-aDswfvjeW8qt9rmJyZoPqtYt_GMUUczLO5TsMkoUfB0FG7aDXYFwAPUjj3X8b_AK0AYwL</recordid><startdate>199601</startdate><enddate>199601</enddate><creator>Venturi, Marco</creator><creator>Bonavina, Luigi</creator><creator>Annoni, Federico</creator><creator>Colombo, Laura</creator><creator>Butera, Caterina</creator><creator>Peracchia, Alberto</creator><creator>Mussini, Emilio</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199601</creationdate><title>Biochemical Assay of Collagen and Elastin in the Normal and Varicose Vein Wall</title><author>Venturi, Marco ; Bonavina, Luigi ; Annoni, Federico ; Colombo, Laura ; Butera, Caterina ; Peracchia, Alberto ; Mussini, Emilio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-85e3f1b7ee3bf35abf39cc483eebbd76ff1d49caf9454ed6ec4d39118bd323093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biochemistry - methods</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology. Vascular system</topic><topic>Collagen - metabolism</topic><topic>Desmosine - metabolism</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Elastin - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Hydroxyproline - metabolism</topic><topic>Isodesmosine - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Muscle, Smooth, Vascular - diagnostic imaging</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Reference Values</topic><topic>Ultrasonography, Doppler, Color</topic><topic>Varicose Veins - diagnostic imaging</topic><topic>Varicose Veins - metabolism</topic><topic>Veins - diagnostic imaging</topic><topic>Veins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Venturi, Marco</creatorcontrib><creatorcontrib>Bonavina, Luigi</creatorcontrib><creatorcontrib>Annoni, Federico</creatorcontrib><creatorcontrib>Colombo, Laura</creatorcontrib><creatorcontrib>Butera, Caterina</creatorcontrib><creatorcontrib>Peracchia, Alberto</creatorcontrib><creatorcontrib>Mussini, Emilio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Venturi, Marco</au><au>Bonavina, Luigi</au><au>Annoni, Federico</au><au>Colombo, Laura</au><au>Butera, Caterina</au><au>Peracchia, Alberto</au><au>Mussini, Emilio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical Assay of Collagen and Elastin in the Normal and Varicose Vein Wall</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>1996-01</date><risdate>1996</risdate><volume>60</volume><issue>1</issue><spage>245</spage><epage>248</epage><pages>245-248</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><coden>JSGRA2</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Alterations of the connective tissue in the varicose vein wall have been noted by several investigators; however, the cause of the vein dilatation has still not been established. The aim of this study was to find a biochemical explanation to the development of varices by evaluating sensitive biochemical markers of collagen and elastin in the varicose vein wall. 4-L-Hydroxyproline (HYP), as a marker of collagen content, and desmosine (DES) and isodesmosine (IDES), as markers of elastin, were measured in 47 macroscopically dilated and 32 nondilated segments of 20 varicose saphenous veins collected from 20 patients with varices. The same measurements were made in 24 fragments of normal saphenous veins collected from 14 patients in whom the vein was removed to be used for graft procedures. HYP (collagen) and DES and IDES (elastin) were determined with a colorimetric method and HPLC, respectively. ANOVA test was used to compare mean values (±SD). HYP and collagen content were similar in varicose and normal veins. There was a significant reduction of both DES and IDES in dilated segments of varicose veins (P< 0.05 vs normal veins and nondilated segments); the ratio of elastin to collagen was lower in varicose than normal veins (P< 0.05), and this reduction was most significant in the dilated segments (P< 0.01 vs normal veins). These results suggest that dilatation of the varicose vein wall may be related to some defect of elastin metabolism. Further studies on the metabolic activity of vein muscle cells are required.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>8592422</pmid><doi>10.1006/jsre.1996.0038</doi><tpages>4</tpages></addata></record> |
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| ispartof | The Journal of surgical research, 1996-01, Vol.60 (1), p.245-248 |
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| language | eng |
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| source | ScienceDirect Journals |
| subjects | Adult Aged Biochemistry - methods Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Collagen - metabolism Desmosine - metabolism Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Elastin - metabolism Female Humans Hydroxyproline - metabolism Isodesmosine - metabolism Male Medical sciences Middle Aged Muscle, Smooth, Vascular - diagnostic imaging Muscle, Smooth, Vascular - metabolism Reference Values Ultrasonography, Doppler, Color Varicose Veins - diagnostic imaging Varicose Veins - metabolism Veins - diagnostic imaging Veins - metabolism |
| title | Biochemical Assay of Collagen and Elastin in the Normal and Varicose Vein Wall |
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