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Inositol 1,4,5-trisphosphate receptor is located to the inner nuclear membrane vindicating regulation of nuclear calcium signaling by inositol 1,4,5-trisphosphate. Discrete distribution of inositol phosphate receptors to inner and outer nuclear membranes
Transient rise in nuclear calcium concentration is implicated in the regulation of events controlling gene expression. Mechanism by which calcium is transported to the nucleus is vehemently debated. Inositol 1,4,5-trisphosphate (InsP3) and inositol-1,3,4,5-tetrakisphosphate (InsP4) receptors have be...
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| Published in: | The Journal of biological chemistry 1996-01, Vol.271 (1), p.478-485 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 485 |
| container_issue | 1 |
| container_start_page | 478 |
| container_title | The Journal of biological chemistry |
| container_volume | 271 |
| creator | Humbert, J P Matter, N Artault, J C Köppler, P Malviya, A N |
| description | Transient rise in nuclear calcium concentration is implicated in the regulation of events controlling gene expression. Mechanism by which calcium is transported to the nucleus is vehemently debated. Inositol 1,4,5-trisphosphate (InsP3) and inositol-1,3,4,5-tetrakisphosphate (InsP4) receptors have been located to the nucleus and their role in nuclear calcium signaling has been proposed. Outer nuclear membrane was separated from the inner membrane. The two membrane preparations were, as best as possible, devoid of cross contamination as attested by marker enzyme activity, Western blotting with antilamin antibody, and electron microscopy. InsP4 receptor and Ca(2+)-ATPase were located to the outer nuclear membrane. InsP3 receptor was located to the inner nuclear membrane. ATP or InsP4 induced nuclear calcium uptake. External free calcium concentration, in the medium bathing the nuclei, determined the choice for ATP or InsP4-mediated calcium transport. We present a mechanistic model for nuclear calcium transport. According to this model, calcium can reach the nucleus envelope either by the action of ATP or InsP4. However, the calcium release from the nucleus envelope to the nucleoplasm is mediated by InsP3 through the activation of InsP3 receptor, which is located to the inner nuclear membrane. The action of InsP3 in this process was instantaneous and transient and was sensitive to heparin. |
| doi_str_mv | 10.1074/jbc.271.1.478 |
| format | article |
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Discrete distribution of inositol phosphate receptors to inner and outer nuclear membranes</title><source>Elsevier ScienceDirect Journals</source><creator>Humbert, J P ; Matter, N ; Artault, J C ; Köppler, P ; Malviya, A N</creator><creatorcontrib>Humbert, J P ; Matter, N ; Artault, J C ; Köppler, P ; Malviya, A N</creatorcontrib><description>Transient rise in nuclear calcium concentration is implicated in the regulation of events controlling gene expression. Mechanism by which calcium is transported to the nucleus is vehemently debated. Inositol 1,4,5-trisphosphate (InsP3) and inositol-1,3,4,5-tetrakisphosphate (InsP4) receptors have been located to the nucleus and their role in nuclear calcium signaling has been proposed. Outer nuclear membrane was separated from the inner membrane. The two membrane preparations were, as best as possible, devoid of cross contamination as attested by marker enzyme activity, Western blotting with antilamin antibody, and electron microscopy. InsP4 receptor and Ca(2+)-ATPase were located to the outer nuclear membrane. InsP3 receptor was located to the inner nuclear membrane. ATP or InsP4 induced nuclear calcium uptake. External free calcium concentration, in the medium bathing the nuclei, determined the choice for ATP or InsP4-mediated calcium transport. We present a mechanistic model for nuclear calcium transport. According to this model, calcium can reach the nucleus envelope either by the action of ATP or InsP4. However, the calcium release from the nucleus envelope to the nucleoplasm is mediated by InsP3 through the activation of InsP3 receptor, which is located to the inner nuclear membrane. The action of InsP3 in this process was instantaneous and transient and was sensitive to heparin.</description><identifier>ISSN: 0021-9258</identifier><identifier>DOI: 10.1074/jbc.271.1.478</identifier><identifier>PMID: 8550605</identifier><language>eng</language><publisher>United States</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; Binding Sites ; Calcium - metabolism ; Calcium Channels - metabolism ; Calcium-Transporting ATPases - metabolism ; Cell Nucleus - metabolism ; Cell Nucleus - ultrastructure ; Inositol 1,4,5-Trisphosphate Receptors ; Inositol Phosphates - metabolism ; Intracellular Membranes - metabolism ; Intracellular Membranes - ultrastructure ; Microscopy, Electron ; Radioligand Assay ; Rats ; Receptors, Cytoplasmic and Nuclear - metabolism ; Signal Transduction ; Tritium</subject><ispartof>The Journal of biological chemistry, 1996-01, Vol.271 (1), p.478-485</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8550605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Humbert, J P</creatorcontrib><creatorcontrib>Matter, N</creatorcontrib><creatorcontrib>Artault, J C</creatorcontrib><creatorcontrib>Köppler, P</creatorcontrib><creatorcontrib>Malviya, A N</creatorcontrib><title>Inositol 1,4,5-trisphosphate receptor is located to the inner nuclear membrane vindicating regulation of nuclear calcium signaling by inositol 1,4,5-trisphosphate. Discrete distribution of inositol phosphate receptors to inner and outer nuclear membranes</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Transient rise in nuclear calcium concentration is implicated in the regulation of events controlling gene expression. Mechanism by which calcium is transported to the nucleus is vehemently debated. Inositol 1,4,5-trisphosphate (InsP3) and inositol-1,3,4,5-tetrakisphosphate (InsP4) receptors have been located to the nucleus and their role in nuclear calcium signaling has been proposed. Outer nuclear membrane was separated from the inner membrane. The two membrane preparations were, as best as possible, devoid of cross contamination as attested by marker enzyme activity, Western blotting with antilamin antibody, and electron microscopy. InsP4 receptor and Ca(2+)-ATPase were located to the outer nuclear membrane. InsP3 receptor was located to the inner nuclear membrane. ATP or InsP4 induced nuclear calcium uptake. External free calcium concentration, in the medium bathing the nuclei, determined the choice for ATP or InsP4-mediated calcium transport. We present a mechanistic model for nuclear calcium transport. According to this model, calcium can reach the nucleus envelope either by the action of ATP or InsP4. However, the calcium release from the nucleus envelope to the nucleoplasm is mediated by InsP3 through the activation of InsP3 receptor, which is located to the inner nuclear membrane. The action of InsP3 in this process was instantaneous and transient and was sensitive to heparin.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Binding Sites</subject><subject>Calcium - metabolism</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Inositol 1,4,5-Trisphosphate Receptors</subject><subject>Inositol Phosphates - metabolism</subject><subject>Intracellular Membranes - metabolism</subject><subject>Intracellular Membranes - ultrastructure</subject><subject>Microscopy, Electron</subject><subject>Radioligand Assay</subject><subject>Rats</subject><subject>Receptors, Cytoplasmic and Nuclear - metabolism</subject><subject>Signal Transduction</subject><subject>Tritium</subject><issn>0021-9258</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkc1PwyAYhzlopk6PHk04eVorUCjt0cyvJSZe9LxQSjcMhQrUZP-8kWXTixpJCG_geX95AADOMcox4vTqtZE54TjHOeXVAThGiOCsJqw6AichvKI0aI0nYFIxhkrEjsHHwrqgozMQz-iMZdHrMKxdmiIq6JVUQ3Qe6gCNk2mrhdHBuFZQW6s8tKM0SnjYq77xwir4rm2rE6jtKnWvRpNKZ6HrvlEpjNRjD4NeWWG2XLNJaX9b5PBGB-lV8ml1SEfN-JX53fZTOWxFd5LCttCN8RfdcAoOO2GCOtuvU_Byd_s8f8gen-4X8-vHbCAFj-kJBeYdrTpECoGbmgpCKaoKIkrGK4qKCtWSU4EUk6JDnWq4wpR0iLVdS1tUTMHlLnfw7m1UIS77dCdlTJJwY1hyXhNS4vJfEHNEOCvrBF7swbHpVbscvO6F3yz3P1t8ArbEqkg</recordid><startdate>19960105</startdate><enddate>19960105</enddate><creator>Humbert, J P</creator><creator>Matter, N</creator><creator>Artault, J C</creator><creator>Köppler, P</creator><creator>Malviya, A N</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>19960105</creationdate><title>Inositol 1,4,5-trisphosphate receptor is located to the inner nuclear membrane vindicating regulation of nuclear calcium signaling by inositol 1,4,5-trisphosphate. Discrete distribution of inositol phosphate receptors to inner and outer nuclear membranes</title><author>Humbert, J P ; Matter, N ; Artault, J C ; Köppler, P ; Malviya, A N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p237t-92a17f48f023a1b94a2440832a6578403809c74a0e5caf0feb7e142f05dfd4d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Binding Sites</topic><topic>Calcium - metabolism</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Inositol 1,4,5-Trisphosphate Receptors</topic><topic>Inositol Phosphates - metabolism</topic><topic>Intracellular Membranes - metabolism</topic><topic>Intracellular Membranes - ultrastructure</topic><topic>Microscopy, Electron</topic><topic>Radioligand Assay</topic><topic>Rats</topic><topic>Receptors, Cytoplasmic and Nuclear - metabolism</topic><topic>Signal Transduction</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Humbert, J P</creatorcontrib><creatorcontrib>Matter, N</creatorcontrib><creatorcontrib>Artault, J C</creatorcontrib><creatorcontrib>Köppler, P</creatorcontrib><creatorcontrib>Malviya, A N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Humbert, J P</au><au>Matter, N</au><au>Artault, J C</au><au>Köppler, P</au><au>Malviya, A N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inositol 1,4,5-trisphosphate receptor is located to the inner nuclear membrane vindicating regulation of nuclear calcium signaling by inositol 1,4,5-trisphosphate. Discrete distribution of inositol phosphate receptors to inner and outer nuclear membranes</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1996-01-05</date><risdate>1996</risdate><volume>271</volume><issue>1</issue><spage>478</spage><epage>485</epage><pages>478-485</pages><issn>0021-9258</issn><abstract>Transient rise in nuclear calcium concentration is implicated in the regulation of events controlling gene expression. Mechanism by which calcium is transported to the nucleus is vehemently debated. Inositol 1,4,5-trisphosphate (InsP3) and inositol-1,3,4,5-tetrakisphosphate (InsP4) receptors have been located to the nucleus and their role in nuclear calcium signaling has been proposed. Outer nuclear membrane was separated from the inner membrane. The two membrane preparations were, as best as possible, devoid of cross contamination as attested by marker enzyme activity, Western blotting with antilamin antibody, and electron microscopy. InsP4 receptor and Ca(2+)-ATPase were located to the outer nuclear membrane. InsP3 receptor was located to the inner nuclear membrane. ATP or InsP4 induced nuclear calcium uptake. External free calcium concentration, in the medium bathing the nuclei, determined the choice for ATP or InsP4-mediated calcium transport. We present a mechanistic model for nuclear calcium transport. According to this model, calcium can reach the nucleus envelope either by the action of ATP or InsP4. However, the calcium release from the nucleus envelope to the nucleoplasm is mediated by InsP3 through the activation of InsP3 receptor, which is located to the inner nuclear membrane. The action of InsP3 in this process was instantaneous and transient and was sensitive to heparin.</abstract><cop>United States</cop><pmid>8550605</pmid><doi>10.1074/jbc.271.1.478</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1996-01, Vol.271 (1), p.478-485 |
| issn | 0021-9258 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphate - metabolism Animals Binding Sites Calcium - metabolism Calcium Channels - metabolism Calcium-Transporting ATPases - metabolism Cell Nucleus - metabolism Cell Nucleus - ultrastructure Inositol 1,4,5-Trisphosphate Receptors Inositol Phosphates - metabolism Intracellular Membranes - metabolism Intracellular Membranes - ultrastructure Microscopy, Electron Radioligand Assay Rats Receptors, Cytoplasmic and Nuclear - metabolism Signal Transduction Tritium |
| title | Inositol 1,4,5-trisphosphate receptor is located to the inner nuclear membrane vindicating regulation of nuclear calcium signaling by inositol 1,4,5-trisphosphate. Discrete distribution of inositol phosphate receptors to inner and outer nuclear membranes |
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