Molecular cloning and RARE cleavage mapping of human 2p, 6q, 8q, 12q, and 18q telomeres

Large terminal fragments of human chromosomes 2p, 6p, 8q, 12q, and 18q were cloned using yeast artificial chromosomes (YACs). RecA-assisted restriction endonuclease (RARE) cleavage analysis of genomic DNA samples from II unrelated individuals using YAC-derived probes confirmed the telomeric localiza...

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Bibliographic Details
Published in:Genome research 1995-10, Vol.5 (3), p.225-232
Main Authors: Macina, R A, Morii, K, Hu, X L, Negorev, D G, Spais, C, Ruthig, L A, Riethman, H C
Format: Article
Language:English
Subjects:
Base Sequence
Chromosomes, Artificial, Yeast
Chromosomes, Human - genetics
Chromosomes, Human - ultrastructure
Chromosomes, Human, Pair 12 - genetics
Chromosomes, Human, Pair 12 - ultrastructure
Chromosomes, Human, Pair 18 - genetics
Chromosomes, Human, Pair 18 - ultrastructure
Chromosomes, Human, Pair 2 - genetics
Chromosomes, Human, Pair 2 - ultrastructure
Chromosomes, Human, Pair 6 - genetics
Chromosomes, Human, Pair 6 - ultrastructure
Chromosomes, Human, Pair 8 - genetics
Chromosomes, Human, Pair 8 - ultrastructure
Cloning, Molecular
Humans
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Polymerase Chain Reaction
Restriction Mapping
Telomere - genetics
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Summary:Large terminal fragments of human chromosomes 2p, 6p, 8q, 12q, and 18q were cloned using yeast artificial chromosomes (YACs). RecA-assisted restriction endonuclease (RARE) cleavage analysis of genomic DNA samples from II unrelated individuals using YAC-derived probes confirmed the telomeric localizations of the half-YACs studied. The cloned fragments provide telomeric closure of maps for the respective chromosome arms and will supply the reagents needed for analyzing and sequencing these distal subtelomeric regions.
ISSN:1088-9051
1549-5469
DOI:10.1101/gr.5.3.225