Cyclosporin-a sensitive induction of NF-AT in murine B cells

Primary B cells are induced to proliferate by crosslinking surface immunoglobulin or by its pharmacological equivalent, phorbol ester and calcium ionophore. However, nuclear responses that have been studied in activated B cells are typically inducible with phorbol esters alone. We show that a factor...

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Bibliographic Details
Published in:Immunity (Cambridge, Mass.) Mass.), 1994-06, Vol.1 (3), p.189-196
Main Authors: Venkataraman, Lakshmi, Francis, Delicia A., Wang, Zihua, Liu, Jialing, Rothstein, Thomas L., Sen, Ranjan
Format: Article
Language:English
Subjects:
Animals
B-Lymphocytes - drug effects
B-Lymphocytes - immunology
B-Lymphocytes - metabolism
Base Sequence
Cell Line
Cyclosporine - pharmacology
DNA Primers - genetics
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
Gene Expression - drug effects
In Vitro Techniques
Lymphocyte Activation
Mice
Molecular Sequence Data
NFATC Transcription Factors
Nuclear Proteins
Transcription Factors - biosynthesis
Transcription Factors - genetics
Transcriptional Activation
Transfection
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Summary:Primary B cells are induced to proliferate by crosslinking surface immunoglobulin or by its pharmacological equivalent, phorbol ester and calcium ionophore. However, nuclear responses that have been studied in activated B cells are typically inducible with phorbol esters alone. We show that a factor, indistinguishable from the nuclear factor of activated T cells (NF-AT), is induced in B cells in response to anti-immunoglobulin signals or the combined action of phorbol ester and ionomycin, but not in response to either reagent alone. The signals necessary for NF-AT induction in B cells, therefore, closely parallel those required to induce B cell proliferation. Transfection analysis shows that B cell NF-AT is a transcriptional activator. Furthermore, NF-AT induction in spienic cells is suppressed by cyclosporin A, suggesting a mechanism by which immunosuppressive agents act on the B cell compartment. We propose that NF-AT should be considered more generally as a nuclear factor of activated lymphoid cells.
ISSN:1074-7613
1097-4180
DOI:10.1016/1074-7613(94)90097-3