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Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients
Human T-lymphotropic virus type III (LAV, HTLV-III) is aetiologically linked to acquired immune deficiency syndrome (AIDS) and persistent general lymphadenopathy (PGL). Specific radioimmunoassays (RIA), enzyme-linked assays, immunofluorescence assays (IFA) and immunoblotting techniques are being use...
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| Published in: | Nature (London) 1985-07, Vol.316 (6023), p.69-72 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c361t-25d8d73ac1f2e89a201b49045ccff420dba0789740b3bf91a3f0c2c4646d78723 |
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| cites | cdi_FETCH-LOGICAL-c361t-25d8d73ac1f2e89a201b49045ccff420dba0789740b3bf91a3f0c2c4646d78723 |
| container_end_page | 72 |
| container_issue | 6023 |
| container_start_page | 69 |
| container_title | Nature (London) |
| container_volume | 316 |
| creator | Weiss, Robin A Clapham, Paul R Cheingsong-Popov, Rachanee Dalgleish, Angus G Carne, Christopher A Weller, Ian V. D Tedder, Richard S |
| description | Human T-lymphotropic virus type III (LAV, HTLV-III) is aetiologically linked to acquired immune deficiency syndrome (AIDS) and persistent general lymphadenopathy (PGL). Specific radioimmunoassays (RIA), enzyme-linked assays, immunofluorescence assays (IFA) and immunoblotting techniques are being used widely to detect serum antibodies to HTLV-III in infected patients and in those at risk of infection. However, these assays do not functionally identify those antibodies that neutralize the infectivity of the virus. We have used three methods of titrating serum neutralizing factors: inhibition of syncytium induction, neutralization of envelope pseudotypes of vesicular stomatitis virus (VSV) and reduction of infectivity of HTLV-III for a cell line permissive to virus replication. We report here that sera from subjects in various disease categories possess only low-level neutralizing activity, even when antibodies to viral membrane antigens are present in high titre. Envelope pseudotypes prepared from four HTLV-III isolates made in three different countries are equally sensitive to neutralization by positive sera, including sera from patients yielding two of the virus isolates. |
| doi_str_mv | 10.1038/316069a0 |
| format | article |
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We have used three methods of titrating serum neutralizing factors: inhibition of syncytium induction, neutralization of envelope pseudotypes of vesicular stomatitis virus (VSV) and reduction of infectivity of HTLV-III for a cell line permissive to virus replication. We report here that sera from subjects in various disease categories possess only low-level neutralizing activity, even when antibodies to viral membrane antigens are present in high titre. Envelope pseudotypes prepared from four HTLV-III isolates made in three different countries are equally sensitive to neutralization by positive sera, including sera from patients yielding two of the virus isolates.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/316069a0</identifier><identifier>PMID: 2989706</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing</publisher><subject>Acquired Immunodeficiency Syndrome - immunology ; AIDS/HIV ; Antibodies, Viral - analysis ; Biological and medical sciences ; Cell Line ; Deltaretrovirus - immunology ; Deltaretrovirus - pathogenicity ; Fundamental and applied biological sciences. 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D</creatorcontrib><creatorcontrib>Tedder, Richard S</creatorcontrib><title>Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients</title><title>Nature (London)</title><addtitle>Nature</addtitle><description>Human T-lymphotropic virus type III (LAV, HTLV-III) is aetiologically linked to acquired immune deficiency syndrome (AIDS) and persistent general lymphadenopathy (PGL). Specific radioimmunoassays (RIA), enzyme-linked assays, immunofluorescence assays (IFA) and immunoblotting techniques are being used widely to detect serum antibodies to HTLV-III in infected patients and in those at risk of infection. However, these assays do not functionally identify those antibodies that neutralize the infectivity of the virus. We have used three methods of titrating serum neutralizing factors: inhibition of syncytium induction, neutralization of envelope pseudotypes of vesicular stomatitis virus (VSV) and reduction of infectivity of HTLV-III for a cell line permissive to virus replication. We report here that sera from subjects in various disease categories possess only low-level neutralizing activity, even when antibodies to viral membrane antigens are present in high titre. Envelope pseudotypes prepared from four HTLV-III isolates made in three different countries are equally sensitive to neutralization by positive sera, including sera from patients yielding two of the virus isolates.</description><subject>Acquired Immunodeficiency Syndrome - immunology</subject><subject>AIDS/HIV</subject><subject>Antibodies, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Deltaretrovirus - immunology</subject><subject>Deltaretrovirus - pathogenicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Neutralization Tests</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Risk</subject><subject>Vesicular stomatitis Indiana virus - immunology</subject><subject>Virology</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNqF0EtP3DAUBWALFQ0DReofKPKiqroJXMeOH8vRtJRIiC6Y7ipFN44tAnnVTpCGX9_QGWbLypbOp2PrEPKJwSUDrq84kyANwhFZMqFkIqRWH8gSINUJaC5PyGmMjwCQMSUWZJEabRTIJflz56YxYFO_4Fj3He09fZha7Ogmabbt8NCPoR9qS5_rMEU6bgdH8zyn5ZZGF_CVr_Lv9xS76v8lCXV8osPc5boxfiTHHpvozvfnGfl9_WOzvkluf_3M16vbxHLJxiTNKl0pjpb51GmDKbBSGBCZtd6LFKoSQc3_FVDy0huG3INNrZBCVkqrlJ-Rr7veIfR_JxfHoq2jdU2DneunWCjJdKYNvAuZ4NoIKWf4bQdt6GMMzhdDqFsM24JB8bp48bb4TD_vO6eyddUB7iee8y_7HKPFxgfsbB0PTGcZMG1mdrFjHY5TcIf88M4__xaQFw</recordid><startdate>19850704</startdate><enddate>19850704</enddate><creator>Weiss, Robin A</creator><creator>Clapham, Paul R</creator><creator>Cheingsong-Popov, Rachanee</creator><creator>Dalgleish, Angus G</creator><creator>Carne, Christopher A</creator><creator>Weller, Ian V. 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| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1985-07, Vol.316 (6023), p.69-72 |
| issn | 0028-0836 1476-4687 |
| language | eng |
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| source | Nature Journals |
| subjects | Acquired Immunodeficiency Syndrome - immunology AIDS/HIV Antibodies, Viral - analysis Biological and medical sciences Cell Line Deltaretrovirus - immunology Deltaretrovirus - pathogenicity Fundamental and applied biological sciences. Psychology Humans Microbiology Neutralization Tests Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Risk Vesicular stomatitis Indiana virus - immunology Virology |
| title | Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients |
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