Anti-inflammatory effects of methanol extracts of the root of Lilium lancifolium on LPS-stimulated Raw264.7 cells

Methanol extract of Lilium lancifolium prevents LPS-induced inflammation in Raw264.7 cells through downregulation of iNOS via suppression of NF-κB activation and nuclear translocation. Lilium lancifolium is commonly used to treat bronchitis, pneumonia, etc. In this study, we investigated the anti-in...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2010-07, Vol.130 (1), p.28-34
Main Authors: Kwon, Ok-Kyoung, Lee, Mee-Young, Yuk, Ji-Eun, Oh, Sei-Ryang, Chin, Young-Won, Lee, Hyeong-Kyu, Ahn, Kyung-Seop
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents - pharmacology
biochemical pathways
Biological and medical sciences
Blotting, Western
Cell Line
cell lines
COX-2
cultured cells
Cyclooxygenase 2 - metabolism
Cytokines - metabolism
Dinoprostone - metabolism
Enzyme-Linked Immunosorbent Assay
General pharmacology
herbal medicines
IL-13
IL-4
Immunohistochemistry
in vitro studies
Inflammation
iNOS
interleukin-4
interleukin-6
interleukins
Lilium
Lilium - chemistry
Lilium lancifolium
Lipopolysaccharides - pharmacology
macrophages
mechanism of action
Medical sciences
medicinal plants
medicinal properties
methanol
Methanol - chemistry
Mice
NF-κB
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - metabolism
nitrous oxide
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phosphorylation
plant extracts
Plant Extracts - pharmacology
Plant Roots - chemistry
prostaglandin synthase
prostaglandins
Protein Kinases - metabolism
protein synthesis
roots
splenocytes
TNF-alpha
tumor necrosis factor-alpha
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Summary:Methanol extract of Lilium lancifolium prevents LPS-induced inflammation in Raw264.7 cells through downregulation of iNOS via suppression of NF-κB activation and nuclear translocation. Lilium lancifolium is commonly used to treat bronchitis, pneumonia, etc. In this study, we investigated the anti-inflammatory effects of methanol extracts of the root of Lilium lancifolium (LL extracts) in LPS-stimulated Raw264.7 cells. Levels of NO, PGE 2 and pro-inflammatory cytokines (IL-6 and TNF-α) in the supernatant fraction were determined using sandwich ELISA. Expression of COX-2 and iNOS, phosphorylation of MAPK subgroups (ERK and JNK), and NF-κB activation in extracts were detected via Western blot and immunocytochemistry assays. The LL extract significantly inhibited NO, PGE 2, IL-6 and TNF-α production in LPS-stimulated cells, and suppressed iNOS and COX-2 expression. A mechanism-based study showed that phosphorylation of ERK1/2 and JNK and translocation of the NF-κB p65 subunit into nuclei were inhibited by the LL extract. Furthermore, interleukin-4 and interleukin-13 production in Con A-induced splenocytes was suppressed. These results indicate that anti-inflammatory effects of methanol extracts from Lilium lancifolium are due to downregulation of iNOS and COX-2 via suppression of NF-κB activation and nuclear translocation as well as blocking of ERK and JNK signaling in LPS-stimulated Raw264.7 cells.
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2010.04.002