Effects of Huanshuai Recipe Oral Liquid (缓衰口服液) on restructuring glomerular microvasculature and expression of vascular endothelial growth factor in subtotal nephrectomized rats

Objectives To explore the effects and significance of Huanshuai Recipe Oral Liquid (缓衰口服液, HSR), a formula with supplementing qi, nourishing blood and activating blood on restructuring glomerular microvasculature and expression of vascular endothelial growth factor (VEGF) in subtotal nephrectomized...

Full description

Saved in:
Bibliographic Details
Published in:Chinese journal of integrative medicine 2010-06, Vol.16 (3), p.239-246
Main Authors: Li, Shen, Rao, Xiang-rong, Song, Jin-ye, Meng, Li-qiang, Qu, Lei, Li, Xiao-mei
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Capillaries - drug effects
Capillaries - metabolism
Capillaries - pathology
Collagen Type IV - metabolism
Creatinine - blood
Drugs, Chinese Herbal - administration & dosage
Drugs, Chinese Herbal - pharmacology
Experimental Research
Extracellular Matrix - drug effects
Extracellular Matrix - metabolism
Fibronectins - metabolism
Immunohistochemistry
Kidney Cortex - drug effects
Kidney Cortex - metabolism
Kidney Cortex - pathology
Kidney Function Tests
Kidney Glomerulus - blood supply
Kidney Glomerulus - drug effects
Kidney Glomerulus - pathology
Kidney Glomerulus - physiopathology
Medicine
Medicine & Public Health
Microvessels - drug effects
Nephrectomy
Proteinuria - blood
Proteinuria - drug therapy
Proteinuria - physiopathology
Rats
Rats, Wistar
Time Factors
Vascular Endothelial Growth Factor A - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objectives To explore the effects and significance of Huanshuai Recipe Oral Liquid (缓衰口服液, HSR), a formula with supplementing qi, nourishing blood and activating blood on restructuring glomerular microvasculature and expression of vascular endothelial growth factor (VEGF) in subtotal nephrectomized (SNX) rats. Methods A total of 76 male Wistar rats were randomly divided into four groups: 16 in the sham-operated group and fed with tap water 10 mL/kg per day; 20 in the model group were operated with 5/6 SNX and fed with tap water 10 mL/ kg per day; 20 SNX rats in the HSR group were treated with HSR 10 mL/kg per day; 20 SNX rats in the losartan group were treated with losartan 40 mg/kg per day. Serum creatinine (SCr) and urinary protein excretion (Upro) were examined at the 2nd, 4th, 8th, and 12th weeks of the treatment, and the remnant kidneys were harvested. Changes in histological microstructure were evaluated using light microscopy, and the expression of VEGF was detected by using ELISA. Results Upro, microvasculature injury and glomerulosclerosis were found to be alleviated in HSR and Losartan groups, respectively. The change of VEGF expression showed positive correlation with glomerular capillary area and peritubular capillary number ( r =0.448, r =0.422, P
ISSN:1672-0415
1993-0402
DOI:10.1007/s11655-010-0239-4