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In Vitro Inhibition of Lipopolysaccharide and Mycobacterium Bovis Bacillus Calmette Guérin-Induced Inflammatory Cytokines and in Vivo Protection from D-Galactosamine/Lps -Mediated Liver Injury by the Medicinal Plant Sclerocarya Birrea
Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory- and bacterial-related diseases. The present study investigated in vitro and in vivo the effects of the stem bark methanol extract of S. birrea. Nitrite, TNF, IL-1β, IL-6 and IL-12p40 production by bone marrow-derived mac...
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| Published in: | International journal of immunopathology and pharmacology 2010-01, Vol.23 (1), p.61-72 |
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| creator | Fotio, A.L. Olleros, M.L. Vesin, D. Tauzin, S. Bisig, R. Dimo, T. Nguelefack, T.B. Dongo, E. Kamtchouing, P. Garcia, I. |
| description | Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory- and bacterial-related diseases. The present study investigated in vitro and in vivo the effects of the stem bark methanol extract of S. birrea. Nitrite, TNF, IL-1β, IL-6 and IL-12p40 production by bone marrow-derived macrophages (BMDM) pre-incubated with or without S. birrea, and stimulated with Lipopolysaccharide (LPS) or infected with live Mycobacterium bovis Bacillus Calmette Guérin (BCG) was evaluated. S. birrea extract inhibited, in a concentration-dependent manner, nitrite, TNF, IL-1β, IL-6 and IL-12p40 production by BMDM stimulated with LPS or infected with live BCG. The iNOS expression was reduced by S. birrea after stimulation of BMDM with LPS. In addition, S. birrea inhibited the nuclear factor κB (NF-κB) activation by both LPS and BCG. The effects of the plant extract were also evaluated in an in vivo model of liver injury induced by D-galactosamine/LPS (D-GalN/LPS) administration in mice. S. birrea limited D-GalN/LPS-liver injury as assessed by a reduction in transaminases and TNF, IL-1β, IL-6 serum levels, and translocation of NF-κB to the nucleus. Taken together, our data indicate that stem bark methanol extract of S. birrea possesses anti-inflammatory properties by inhibiting NF-κB activation and cytokine release induced by inflammatory or infectious stimuli. |
| doi_str_mv | 10.1177/039463201002300106 |
| format | article |
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The present study investigated in vitro and in vivo the effects of the stem bark methanol extract of S. birrea. Nitrite, TNF, IL-1β, IL-6 and IL-12p40 production by bone marrow-derived macrophages (BMDM) pre-incubated with or without S. birrea, and stimulated with Lipopolysaccharide (LPS) or infected with live Mycobacterium bovis Bacillus Calmette Guérin (BCG) was evaluated. S. birrea extract inhibited, in a concentration-dependent manner, nitrite, TNF, IL-1β, IL-6 and IL-12p40 production by BMDM stimulated with LPS or infected with live BCG. The iNOS expression was reduced by S. birrea after stimulation of BMDM with LPS. In addition, S. birrea inhibited the nuclear factor κB (NF-κB) activation by both LPS and BCG. The effects of the plant extract were also evaluated in an in vivo model of liver injury induced by D-galactosamine/LPS (D-GalN/LPS) administration in mice. S. birrea limited D-GalN/LPS-liver injury as assessed by a reduction in transaminases and TNF, IL-1β, IL-6 serum levels, and translocation of NF-κB to the nucleus. Taken together, our data indicate that stem bark methanol extract of S. birrea possesses anti-inflammatory properties by inhibiting NF-κB activation and cytokine release induced by inflammatory or infectious stimuli.</description><identifier>ISSN: 0394-6320</identifier><identifier>EISSN: 2058-7384</identifier><identifier>DOI: 10.1177/039463201002300106</identifier><identifier>PMID: 20377995</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Active Transport, Cell Nucleus - drug effects ; Anacardiaceae ; Animals ; Anti-Inflammatory Agents - pharmacology ; Cytokines - antagonists & inhibitors ; Cytokines - biosynthesis ; Female ; Galactosamine - toxicity ; Lipopolysaccharides - toxicity ; Liver Failure - chemically induced ; Liver Failure - prevention & control ; Macrophages - drug effects ; Macrophages - immunology ; Male ; Mice ; Mice, Inbred BALB C ; Mycobacterium bovis ; Mycobacterium bovis - pathogenicity ; NF-kappa B - antagonists & inhibitors ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase Type II - antagonists & inhibitors ; Plant Extracts - pharmacology ; Sclerocarya birrea</subject><ispartof>International journal of immunopathology and pharmacology, 2010-01, Vol.23 (1), p.61-72</ispartof><rights>2010 SAGE Publications</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-f0654ccaf296e2d32d11045b32c2c5df3b04944ed415784d19cc9e81d53cccb03</citedby><cites>FETCH-LOGICAL-c418t-f0654ccaf296e2d32d11045b32c2c5df3b04944ed415784d19cc9e81d53cccb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/039463201002300106$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/039463201002300106$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>315,786,790,21994,27886,27957,27958,44980,45368</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/039463201002300106?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20377995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fotio, A.L.</creatorcontrib><creatorcontrib>Olleros, M.L.</creatorcontrib><creatorcontrib>Vesin, D.</creatorcontrib><creatorcontrib>Tauzin, S.</creatorcontrib><creatorcontrib>Bisig, R.</creatorcontrib><creatorcontrib>Dimo, T.</creatorcontrib><creatorcontrib>Nguelefack, T.B.</creatorcontrib><creatorcontrib>Dongo, E.</creatorcontrib><creatorcontrib>Kamtchouing, P.</creatorcontrib><creatorcontrib>Garcia, I.</creatorcontrib><title>In Vitro Inhibition of Lipopolysaccharide and Mycobacterium Bovis Bacillus Calmette Guérin-Induced Inflammatory Cytokines and in Vivo Protection from D-Galactosamine/Lps -Mediated Liver Injury by the Medicinal Plant Sclerocarya Birrea</title><title>International journal of immunopathology and pharmacology</title><addtitle>Int J Immunopathol Pharmacol</addtitle><description>Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory- and bacterial-related diseases. The present study investigated in vitro and in vivo the effects of the stem bark methanol extract of S. birrea. Nitrite, TNF, IL-1β, IL-6 and IL-12p40 production by bone marrow-derived macrophages (BMDM) pre-incubated with or without S. birrea, and stimulated with Lipopolysaccharide (LPS) or infected with live Mycobacterium bovis Bacillus Calmette Guérin (BCG) was evaluated. S. birrea extract inhibited, in a concentration-dependent manner, nitrite, TNF, IL-1β, IL-6 and IL-12p40 production by BMDM stimulated with LPS or infected with live BCG. The iNOS expression was reduced by S. birrea after stimulation of BMDM with LPS. In addition, S. birrea inhibited the nuclear factor κB (NF-κB) activation by both LPS and BCG. The effects of the plant extract were also evaluated in an in vivo model of liver injury induced by D-galactosamine/LPS (D-GalN/LPS) administration in mice. S. birrea limited D-GalN/LPS-liver injury as assessed by a reduction in transaminases and TNF, IL-1β, IL-6 serum levels, and translocation of NF-κB to the nucleus. Taken together, our data indicate that stem bark methanol extract of S. birrea possesses anti-inflammatory properties by inhibiting NF-κB activation and cytokine release induced by inflammatory or infectious stimuli.</description><subject>Active Transport, Cell Nucleus - drug effects</subject><subject>Anacardiaceae</subject><subject>Animals</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Cytokines - antagonists & inhibitors</subject><subject>Cytokines - biosynthesis</subject><subject>Female</subject><subject>Galactosamine - toxicity</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Liver Failure - chemically induced</subject><subject>Liver Failure - prevention & control</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mycobacterium bovis</subject><subject>Mycobacterium bovis - pathogenicity</subject><subject>NF-kappa B - antagonists & inhibitors</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase Type II - antagonists & inhibitors</subject><subject>Plant Extracts - pharmacology</subject><subject>Sclerocarya birrea</subject><issn>0394-6320</issn><issn>2058-7384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFks2O0zAQxyMEYlfLvgAH5BunUH-lSY60LKVSV6zExzVyxhPq4sTFdirlkXgOHoeXwNkuXJDgNAf_5vf32JNlzxl9xVhZLqio5VJwyijlgqayfJRdclpUeSkq-Ti7nIF8Ji6y6xAOdGaELCr2NLvgVJRlXReX2c_tQD6b6B3ZDnvTmmjcQFxHdubojs5OQQHslTcaiRo0uZ3AtQoiejP2ZOVOJpCVAmPtGMha2R5jRLIZf3z3Zsi3gx4BdVJ3VvW9is5PZD1F99UMGO6FZo4_OXLnXUS4T--868mbfKNsCnJB9Qle7I6B5LeojYpJuDMn9El7GJOwnUjcI5kPwQzKkjurhkg-gEXvQPlJkZXxHtWz7EmnbMDrh3qVfXp783H9Lt-932zXr3c5SFbFvKPLQgKojtdL5FpwzRiVRSs4cCh0J1oqaylRS1aUldSsBqixYroQANBScZW9PHuP3n0bMcSmNwHQpmuhG0NTyiXjlaj5_0khClrVokgkP5PgXQgeu-boTZ-Gaxht5oVo_l6I1PTiQT-2Peo_Lb-_PwGLMxDUF2wObvTp_cK_lL8AdU3Cvw</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Fotio, A.L.</creator><creator>Olleros, M.L.</creator><creator>Vesin, D.</creator><creator>Tauzin, S.</creator><creator>Bisig, R.</creator><creator>Dimo, T.</creator><creator>Nguelefack, T.B.</creator><creator>Dongo, E.</creator><creator>Kamtchouing, P.</creator><creator>Garcia, I.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20100101</creationdate><title>In Vitro Inhibition of Lipopolysaccharide and Mycobacterium Bovis Bacillus Calmette Guérin-Induced Inflammatory Cytokines and in Vivo Protection from D-Galactosamine/Lps -Mediated Liver Injury by the Medicinal Plant Sclerocarya Birrea</title><author>Fotio, A.L. ; Olleros, M.L. ; Vesin, D. ; Tauzin, S. ; Bisig, R. ; Dimo, T. ; Nguelefack, T.B. ; Dongo, E. ; Kamtchouing, P. ; Garcia, I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-f0654ccaf296e2d32d11045b32c2c5df3b04944ed415784d19cc9e81d53cccb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Active Transport, Cell Nucleus - drug effects</topic><topic>Anacardiaceae</topic><topic>Animals</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Cytokines - antagonists & inhibitors</topic><topic>Cytokines - biosynthesis</topic><topic>Female</topic><topic>Galactosamine - toxicity</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Liver Failure - chemically induced</topic><topic>Liver Failure - prevention & control</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mycobacterium bovis</topic><topic>Mycobacterium bovis - pathogenicity</topic><topic>NF-kappa B - antagonists & inhibitors</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase Type II - antagonists & inhibitors</topic><topic>Plant Extracts - pharmacology</topic><topic>Sclerocarya birrea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fotio, A.L.</creatorcontrib><creatorcontrib>Olleros, M.L.</creatorcontrib><creatorcontrib>Vesin, D.</creatorcontrib><creatorcontrib>Tauzin, S.</creatorcontrib><creatorcontrib>Bisig, R.</creatorcontrib><creatorcontrib>Dimo, T.</creatorcontrib><creatorcontrib>Nguelefack, T.B.</creatorcontrib><creatorcontrib>Dongo, E.</creatorcontrib><creatorcontrib>Kamtchouing, P.</creatorcontrib><creatorcontrib>Garcia, I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International journal of immunopathology and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Fotio, A.L.</au><au>Olleros, M.L.</au><au>Vesin, D.</au><au>Tauzin, S.</au><au>Bisig, R.</au><au>Dimo, T.</au><au>Nguelefack, T.B.</au><au>Dongo, E.</au><au>Kamtchouing, P.</au><au>Garcia, I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Inhibition of Lipopolysaccharide and Mycobacterium Bovis Bacillus Calmette Guérin-Induced Inflammatory Cytokines and in Vivo Protection from D-Galactosamine/Lps -Mediated Liver Injury by the Medicinal Plant Sclerocarya Birrea</atitle><jtitle>International journal of immunopathology and pharmacology</jtitle><addtitle>Int J Immunopathol Pharmacol</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>23</volume><issue>1</issue><spage>61</spage><epage>72</epage><pages>61-72</pages><issn>0394-6320</issn><eissn>2058-7384</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory- and bacterial-related diseases. The present study investigated in vitro and in vivo the effects of the stem bark methanol extract of S. birrea. Nitrite, TNF, IL-1β, IL-6 and IL-12p40 production by bone marrow-derived macrophages (BMDM) pre-incubated with or without S. birrea, and stimulated with Lipopolysaccharide (LPS) or infected with live Mycobacterium bovis Bacillus Calmette Guérin (BCG) was evaluated. S. birrea extract inhibited, in a concentration-dependent manner, nitrite, TNF, IL-1β, IL-6 and IL-12p40 production by BMDM stimulated with LPS or infected with live BCG. The iNOS expression was reduced by S. birrea after stimulation of BMDM with LPS. In addition, S. birrea inhibited the nuclear factor κB (NF-κB) activation by both LPS and BCG. The effects of the plant extract were also evaluated in an in vivo model of liver injury induced by D-galactosamine/LPS (D-GalN/LPS) administration in mice. S. birrea limited D-GalN/LPS-liver injury as assessed by a reduction in transaminases and TNF, IL-1β, IL-6 serum levels, and translocation of NF-κB to the nucleus. Taken together, our data indicate that stem bark methanol extract of S. birrea possesses anti-inflammatory properties by inhibiting NF-κB activation and cytokine release induced by inflammatory or infectious stimuli.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>20377995</pmid><doi>10.1177/039463201002300106</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Active Transport, Cell Nucleus - drug effects Anacardiaceae Animals Anti-Inflammatory Agents - pharmacology Cytokines - antagonists & inhibitors Cytokines - biosynthesis Female Galactosamine - toxicity Lipopolysaccharides - toxicity Liver Failure - chemically induced Liver Failure - prevention & control Macrophages - drug effects Macrophages - immunology Male Mice Mice, Inbred BALB C Mycobacterium bovis Mycobacterium bovis - pathogenicity NF-kappa B - antagonists & inhibitors Nitric Oxide - biosynthesis Nitric Oxide Synthase Type II - antagonists & inhibitors Plant Extracts - pharmacology Sclerocarya birrea |
| title | In Vitro Inhibition of Lipopolysaccharide and Mycobacterium Bovis Bacillus Calmette Guérin-Induced Inflammatory Cytokines and in Vivo Protection from D-Galactosamine/Lps -Mediated Liver Injury by the Medicinal Plant Sclerocarya Birrea |
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