Photosensitizing Effect of Hematoporphyrin IX on Immature Stages of Ceratitis capitata (Diptera: Tephritidae)

Immature stages of Ceratitis capitata were tested as a model for hematoporphyrin IX (HP IX) phototoxicity. The lethal concentration 50 (LC50) of HP IX in the food was determined during postembryonic development until adult emergence as 0.173 mm (95% CI: 0.138–0.209). The corresponding HP IX LC50 dur...

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Bibliographic Details
Published in:Photochemistry and photobiology 2010-05, Vol.86 (3), p.639-644
Main Authors: Pujol-Lereis, Luciana Mercedes, Massaldi, Ana, Rabossi, Alejandro, Quesada-Allué, Luis Alberto
Format: Article
Language:English
Subjects:
Animals
Brain - metabolism
Ceratitis capitata
Ceratitis capitata - drug effects
Ceratitis capitata - radiation effects
Diptera
Dyes
Hematoporphyrins - pharmacology
Insects
Intestines - metabolism
Larva - drug effects
Larva - radiation effects
Lipid Peroxidation - drug effects
Lipid Peroxidation - radiation effects
Mortality
Oxidation
Photosensitizing Agents - pharmacology
Reactive Oxygen Species - analysis
Tephritidae
Thiobarbiturates - analysis
Toxicity
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Summary:Immature stages of Ceratitis capitata were tested as a model for hematoporphyrin IX (HP IX) phototoxicity. The lethal concentration 50 (LC50) of HP IX in the food was determined during postembryonic development until adult emergence as 0.173 mm (95% CI: 0.138–0.209). The corresponding HP IX LC50 during the dispersal period alone was 0.536 mm (95% CI: 0.450–0.633). HP IX toxicity was compared against Phloxine B (PhB) (0.5 mm). HP IX elicited a mortality of 90.87%, which was mainly concentrated during prepupal and early pupal stages. PhB mortality was much lower (56.88%) and occurred mainly during the adult pharate stage. A direct correlation between light‐dependent HP IX mortality, evidence of reactive oxygen species (ROS) and lipid peroxidation (conjugated dienes and thiobarbituric acid reactive substances) was established in C. capitata larvae. ROS were found to be very significant in both the brain and in the gut.
ISSN:0031-8655
1751-1097
DOI:10.1111/j.1751-1097.2009.00704.x