Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection

This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass...

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Bibliographic Details
Published in:Biomedical microdevices 2009-12, Vol.11 (6), p.1169-1174
Main Authors: Liu, Xinyu, Sun, Yu
Format: Article
Language:eng
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Summary:This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development.
ISSN:1387-2176
1572-8781