The centromere geometry essential for keeping mitosis error free is controlled by spindle forces

Accurate segregation of chromosomes, essential for the stability of the genome, depends on 'bi-orientation'-simultaneous attachment of each individual chromosome to both poles of the mitotic spindle. On bi-oriented chromosomes, kinetochores (macromolecular complexes that attach the chromos...

Full description

Saved in:
Bibliographic Details
Published in:Nature 2007-11, Vol.450 (7170), p.745-749
Main Authors: Kapoor, Tarun M, Khodjakov, Alexey, Lon arek, Jadranka, Kisurina-Evgenieva, Olga, Vinogradova, Tatiana, Hergert, Polla, La Terra, Sabrina
Format: Article
Language:English
Subjects:
Animals
Cell cycle
Cell Line
Centromere - metabolism
Chromatids - drug effects
Chromatids - metabolism
Chromosome Segregation - drug effects
Chromosomes
Elastic properties
Female
Kinetochores - metabolism
Macropodidae
Mammals
Microtubules - physiology
Mitosis
Proteins
Pyrimidines - pharmacology
Spindle Apparatus - metabolism
Synteny
Thiones - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c605t-f1100277eef57e700bf69d4f9e7a65ce291d858d4aee331627983e32ec63ff353
cites cdi_FETCH-LOGICAL-c605t-f1100277eef57e700bf69d4f9e7a65ce291d858d4aee331627983e32ec63ff353
container_end_page 749
container_issue 7170
container_start_page 745
container_title Nature
container_volume 450
creator Kapoor, Tarun M
Khodjakov, Alexey
Lon arek, Jadranka
Kisurina-Evgenieva, Olga
Vinogradova, Tatiana
Hergert, Polla
La Terra, Sabrina
description Accurate segregation of chromosomes, essential for the stability of the genome, depends on 'bi-orientation'-simultaneous attachment of each individual chromosome to both poles of the mitotic spindle. On bi-oriented chromosomes, kinetochores (macromolecular complexes that attach the chromosome to the spindle) reside on the opposite sides of the chromosome's centromere. In contrast, sister kinetochores shift towards one side of the centromere on 'syntelic' chromosomes that erroneously attach to one spindle pole with both sister kinetochores. Syntelic attachments often arise during spindle assembly and must be corrected to prevent chromosome loss. It is assumed that restoration of proper centromere architecture occurs automatically owing to elastic properties of the centromere. Here we test this assumption by combining laser microsurgery and chemical biology assays in cultured mammalian cells. We find that kinetochores of syntelic chromosomes remain juxtaposed on detachment from spindle microtubules. These findings reveal that correction of syntelic attachments involves an extra step that has previously been overlooked: external forces must be applied to move sister kinetochores to the opposite sides of the centromere. Furthermore, we demonstrate that the shape of the centromere is important for spindle assembly, because bipolar spindles do not form in cells lacking centrosomes when multiple chromosomes with juxtaposed kinetochores are present. Thus, proper architecture of the centromere makes an important contribution to achieving high fidelity of chromosome segregation.
doi_str_mv 10.1038/nature06344
format article
fullrecord <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_743499439</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A189749049</galeid><sourcerecordid>A189749049</sourcerecordid><originalsourceid>FETCH-LOGICAL-c605t-f1100277eef57e700bf69d4f9e7a65ce291d858d4aee331627983e32ec63ff353</originalsourceid><addsrcrecordid>eNqF0s1rFTEQAPAgin1WT94lelCKbJ1svo-laFsoCLU9x7zdyXPrfjyTXfD992a7D1s82NOQ4TfDTBhCXjM4ZsDNp96PU0RQXIgnZMWEVoVQRj8lK4DSFGC4OiAvUroFAMm0eE4OmAGhBFMr8v36B9IK-zEOHUakG8xxjDuKKeVs41sahkh_Im6bfkO7ZhxSkyjGmLMhItL8qoa5vm2xpusdTVnWLc51FaaX5FnwbcJX-3hIbr58vj49Ly6_nl2cnlwWlQI5FoGxPK3WiEFq1ADroGwtgkXtlaywtKw20tTCI3LOVKmt4chLrBQPgUt-SD4sfbdx-DVhGl3XpArb1vc4TMlpwYW1gtss3_9X8ty8ZNI8CksQJUjFM3z3D7wdptjndWcjlTLl3O1oQRvfomv6-dPw97jxU0ru4tuVO2GWC63Z3TKPWmO1sCDmdT4utopDShGD28am83HnGLj5QNyDA8n6zX7Uad1hfW_3F5HB2wUsVX_BwyZ_AJqVwVM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>204566828</pqid></control><display><type>article</type><title>The centromere geometry essential for keeping mitosis error free is controlled by spindle forces</title><source>Nature</source><creator>Kapoor, Tarun M ; Khodjakov, Alexey ; Lon arek, Jadranka ; Kisurina-Evgenieva, Olga ; Vinogradova, Tatiana ; Hergert, Polla ; La Terra, Sabrina</creator><creatorcontrib>Kapoor, Tarun M ; Khodjakov, Alexey ; Lon arek, Jadranka ; Kisurina-Evgenieva, Olga ; Vinogradova, Tatiana ; Hergert, Polla ; La Terra, Sabrina</creatorcontrib><description>Accurate segregation of chromosomes, essential for the stability of the genome, depends on 'bi-orientation'-simultaneous attachment of each individual chromosome to both poles of the mitotic spindle. On bi-oriented chromosomes, kinetochores (macromolecular complexes that attach the chromosome to the spindle) reside on the opposite sides of the chromosome's centromere. In contrast, sister kinetochores shift towards one side of the centromere on 'syntelic' chromosomes that erroneously attach to one spindle pole with both sister kinetochores. Syntelic attachments often arise during spindle assembly and must be corrected to prevent chromosome loss. It is assumed that restoration of proper centromere architecture occurs automatically owing to elastic properties of the centromere. Here we test this assumption by combining laser microsurgery and chemical biology assays in cultured mammalian cells. We find that kinetochores of syntelic chromosomes remain juxtaposed on detachment from spindle microtubules. These findings reveal that correction of syntelic attachments involves an extra step that has previously been overlooked: external forces must be applied to move sister kinetochores to the opposite sides of the centromere. Furthermore, we demonstrate that the shape of the centromere is important for spindle assembly, because bipolar spindles do not form in cells lacking centrosomes when multiple chromosomes with juxtaposed kinetochores are present. Thus, proper architecture of the centromere makes an important contribution to achieving high fidelity of chromosome segregation.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/nature06344</identifier><identifier>PMID: 18046416</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Animals ; Cell cycle ; Cell Line ; Centromere - metabolism ; Chromatids - drug effects ; Chromatids - metabolism ; Chromosome Segregation - drug effects ; Chromosomes ; Elastic properties ; Female ; Kinetochores - metabolism ; Macropodidae ; Mammals ; Microtubules - physiology ; Mitosis ; Proteins ; Pyrimidines - pharmacology ; Spindle Apparatus - metabolism ; Synteny ; Thiones - pharmacology</subject><ispartof>Nature, 2007-11, Vol.450 (7170), p.745-749</ispartof><rights>COPYRIGHT 2007 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Nov 29, 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c605t-f1100277eef57e700bf69d4f9e7a65ce291d858d4aee331627983e32ec63ff353</citedby><cites>FETCH-LOGICAL-c605t-f1100277eef57e700bf69d4f9e7a65ce291d858d4aee331627983e32ec63ff353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,2734,27936,27937</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18046416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kapoor, Tarun M</creatorcontrib><creatorcontrib>Khodjakov, Alexey</creatorcontrib><creatorcontrib>Lon arek, Jadranka</creatorcontrib><creatorcontrib>Kisurina-Evgenieva, Olga</creatorcontrib><creatorcontrib>Vinogradova, Tatiana</creatorcontrib><creatorcontrib>Hergert, Polla</creatorcontrib><creatorcontrib>La Terra, Sabrina</creatorcontrib><title>The centromere geometry essential for keeping mitosis error free is controlled by spindle forces</title><title>Nature</title><addtitle>Nature</addtitle><description>Accurate segregation of chromosomes, essential for the stability of the genome, depends on 'bi-orientation'-simultaneous attachment of each individual chromosome to both poles of the mitotic spindle. On bi-oriented chromosomes, kinetochores (macromolecular complexes that attach the chromosome to the spindle) reside on the opposite sides of the chromosome's centromere. In contrast, sister kinetochores shift towards one side of the centromere on 'syntelic' chromosomes that erroneously attach to one spindle pole with both sister kinetochores. Syntelic attachments often arise during spindle assembly and must be corrected to prevent chromosome loss. It is assumed that restoration of proper centromere architecture occurs automatically owing to elastic properties of the centromere. Here we test this assumption by combining laser microsurgery and chemical biology assays in cultured mammalian cells. We find that kinetochores of syntelic chromosomes remain juxtaposed on detachment from spindle microtubules. These findings reveal that correction of syntelic attachments involves an extra step that has previously been overlooked: external forces must be applied to move sister kinetochores to the opposite sides of the centromere. Furthermore, we demonstrate that the shape of the centromere is important for spindle assembly, because bipolar spindles do not form in cells lacking centrosomes when multiple chromosomes with juxtaposed kinetochores are present. Thus, proper architecture of the centromere makes an important contribution to achieving high fidelity of chromosome segregation.</description><subject>Animals</subject><subject>Cell cycle</subject><subject>Cell Line</subject><subject>Centromere - metabolism</subject><subject>Chromatids - drug effects</subject><subject>Chromatids - metabolism</subject><subject>Chromosome Segregation - drug effects</subject><subject>Chromosomes</subject><subject>Elastic properties</subject><subject>Female</subject><subject>Kinetochores - metabolism</subject><subject>Macropodidae</subject><subject>Mammals</subject><subject>Microtubules - physiology</subject><subject>Mitosis</subject><subject>Proteins</subject><subject>Pyrimidines - pharmacology</subject><subject>Spindle Apparatus - metabolism</subject><subject>Synteny</subject><subject>Thiones - pharmacology</subject><issn>0028-0836</issn><issn>1476-4687</issn><issn>1476-4679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqF0s1rFTEQAPAgin1WT94lelCKbJ1svo-laFsoCLU9x7zdyXPrfjyTXfD992a7D1s82NOQ4TfDTBhCXjM4ZsDNp96PU0RQXIgnZMWEVoVQRj8lK4DSFGC4OiAvUroFAMm0eE4OmAGhBFMr8v36B9IK-zEOHUakG8xxjDuKKeVs41sahkh_Im6bfkO7ZhxSkyjGmLMhItL8qoa5vm2xpusdTVnWLc51FaaX5FnwbcJX-3hIbr58vj49Ly6_nl2cnlwWlQI5FoGxPK3WiEFq1ADroGwtgkXtlaywtKw20tTCI3LOVKmt4chLrBQPgUt-SD4sfbdx-DVhGl3XpArb1vc4TMlpwYW1gtss3_9X8ty8ZNI8CksQJUjFM3z3D7wdptjndWcjlTLl3O1oQRvfomv6-dPw97jxU0ru4tuVO2GWC63Z3TKPWmO1sCDmdT4utopDShGD28am83HnGLj5QNyDA8n6zX7Uad1hfW_3F5HB2wUsVX_BwyZ_AJqVwVM</recordid><startdate>20071129</startdate><enddate>20071129</enddate><creator>Kapoor, Tarun M</creator><creator>Khodjakov, Alexey</creator><creator>Lon arek, Jadranka</creator><creator>Kisurina-Evgenieva, Olga</creator><creator>Vinogradova, Tatiana</creator><creator>Hergert, Polla</creator><creator>La Terra, Sabrina</creator><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T5</scope><scope>7TG</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>S0X</scope><scope>SOI</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>20071129</creationdate><title>The centromere geometry essential for keeping mitosis error free is controlled by spindle forces</title><author>Kapoor, Tarun M ; Khodjakov, Alexey ; Lon arek, Jadranka ; Kisurina-Evgenieva, Olga ; Vinogradova, Tatiana ; Hergert, Polla ; La Terra, Sabrina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c605t-f1100277eef57e700bf69d4f9e7a65ce291d858d4aee331627983e32ec63ff353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Cell cycle</topic><topic>Cell Line</topic><topic>Centromere - metabolism</topic><topic>Chromatids - drug effects</topic><topic>Chromatids - metabolism</topic><topic>Chromosome Segregation - drug effects</topic><topic>Chromosomes</topic><topic>Elastic properties</topic><topic>Female</topic><topic>Kinetochores - metabolism</topic><topic>Macropodidae</topic><topic>Mammals</topic><topic>Microtubules - physiology</topic><topic>Mitosis</topic><topic>Proteins</topic><topic>Pyrimidines - pharmacology</topic><topic>Spindle Apparatus - metabolism</topic><topic>Synteny</topic><topic>Thiones - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kapoor, Tarun M</creatorcontrib><creatorcontrib>Khodjakov, Alexey</creatorcontrib><creatorcontrib>Lon arek, Jadranka</creatorcontrib><creatorcontrib>Kisurina-Evgenieva, Olga</creatorcontrib><creatorcontrib>Vinogradova, Tatiana</creatorcontrib><creatorcontrib>Hergert, Polla</creatorcontrib><creatorcontrib>La Terra, Sabrina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Database‎ (1962 - current)</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric &amp; Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agricultural Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Psychology Database (ProQuest)</collection><collection>Research Library (ProQuest)</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric &amp; Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>University of Michigan</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>Environment Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics &amp; Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology &amp; Engineering</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts – Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><jtitle>Nature</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kapoor, Tarun M</au><au>Khodjakov, Alexey</au><au>Lon arek, Jadranka</au><au>Kisurina-Evgenieva, Olga</au><au>Vinogradova, Tatiana</au><au>Hergert, Polla</au><au>La Terra, Sabrina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The centromere geometry essential for keeping mitosis error free is controlled by spindle forces</atitle><jtitle>Nature</jtitle><addtitle>Nature</addtitle><date>2007-11-29</date><risdate>2007</risdate><volume>450</volume><issue>7170</issue><spage>745</spage><epage>749</epage><pages>745-749</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><eissn>1476-4679</eissn><coden>NATUAS</coden><abstract>Accurate segregation of chromosomes, essential for the stability of the genome, depends on 'bi-orientation'-simultaneous attachment of each individual chromosome to both poles of the mitotic spindle. On bi-oriented chromosomes, kinetochores (macromolecular complexes that attach the chromosome to the spindle) reside on the opposite sides of the chromosome's centromere. In contrast, sister kinetochores shift towards one side of the centromere on 'syntelic' chromosomes that erroneously attach to one spindle pole with both sister kinetochores. Syntelic attachments often arise during spindle assembly and must be corrected to prevent chromosome loss. It is assumed that restoration of proper centromere architecture occurs automatically owing to elastic properties of the centromere. Here we test this assumption by combining laser microsurgery and chemical biology assays in cultured mammalian cells. We find that kinetochores of syntelic chromosomes remain juxtaposed on detachment from spindle microtubules. These findings reveal that correction of syntelic attachments involves an extra step that has previously been overlooked: external forces must be applied to move sister kinetochores to the opposite sides of the centromere. Furthermore, we demonstrate that the shape of the centromere is important for spindle assembly, because bipolar spindles do not form in cells lacking centrosomes when multiple chromosomes with juxtaposed kinetochores are present. Thus, proper architecture of the centromere makes an important contribution to achieving high fidelity of chromosome segregation.</abstract><cop>England</cop><pub>Nature Publishing Group</pub><pmid>18046416</pmid><doi>10.1038/nature06344</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0028-0836
ispartof Nature, 2007-11, Vol.450 (7170), p.745-749
issn 0028-0836
1476-4687
1476-4679
language eng
recordid cdi_proquest_miscellaneous_743499439
source Nature
subjects Animals
Cell cycle
Cell Line
Centromere - metabolism
Chromatids - drug effects
Chromatids - metabolism
Chromosome Segregation - drug effects
Chromosomes
Elastic properties
Female
Kinetochores - metabolism
Macropodidae
Mammals
Microtubules - physiology
Mitosis
Proteins
Pyrimidines - pharmacology
Spindle Apparatus - metabolism
Synteny
Thiones - pharmacology
title The centromere geometry essential for keeping mitosis error free is controlled by spindle forces
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-11-13T14%3A54%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20centromere%20geometry%20essential%20for%20keeping%20mitosis%20error%20free%20is%20controlled%20by%20spindle%20forces&rft.jtitle=Nature&rft.au=Kapoor,%20Tarun%20M&rft.date=2007-11-29&rft.volume=450&rft.issue=7170&rft.spage=745&rft.epage=749&rft.pages=745-749&rft.issn=0028-0836&rft.eissn=1476-4687&rft.coden=NATUAS&rft_id=info:doi/10.1038/nature06344&rft_dat=%3Cgale_proqu%3EA189749049%3C/gale_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c605t-f1100277eef57e700bf69d4f9e7a65ce291d858d4aee331627983e32ec63ff353%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=204566828&rft_id=info:pmid/18046416&rft_galeid=A189749049&rfr_iscdi=true