Alterations of transcription during heat shock of Saccharomyces cerevisiae

The pattern of polyadenylated RNA of the yeast Saccharomyces cerevisiae changes dramatically upon heat shock. By in vitro translation, we have demonstrated that a 2.9-kilobase heat shock RNA encodes the Mr = 90,000 yeast heat shock protein. Heat shock-responsive genes have been isolated by different...

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Published in:The Journal of biological chemistry 1982-07, Vol.257 (14), p.8405-8411
Main Authors: Finkelstein, D B, Strausberg, S, McAlister, L
Format: Article
Language:English
Subjects:
Bacteriophage lambda - genetics
DNA Restriction Enzymes
DNA, Recombinant - metabolism
Escherichia coli - genetics
Heat-Shock Proteins
Hot Temperature
Nucleic Acid Hybridization
Poly A - genetics
Poly A - isolation & purification
Protein Biosynthesis
Proteins - genetics
RNA - genetics
RNA - isolation & purification
RNA, Messenger
Saccharomyces cerevisiae - genetics
Transcription, Genetic
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cited_by cdi_FETCH-LOGICAL-c454t-61464b333d02ea2263f1b07d6a60ace1b207ebb63161fa0de4fbc628bbb27e793
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Strausberg, S
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description The pattern of polyadenylated RNA of the yeast Saccharomyces cerevisiae changes dramatically upon heat shock. By in vitro translation, we have demonstrated that a 2.9-kilobase heat shock RNA encodes the Mr = 90,000 yeast heat shock protein. Heat shock-responsive genes have been isolated by differential plaque filter hybridization of a recombinant library of yeast DNA inserted in the vector lambda Charon 4. The putative yeast gene products of a number of these recombinants molecules has been determined by in vitro translation of hybrid-selected RNA. We have used one of these hybrid phages (lambda Yhsil) to demonstrate that the heat shock-induced alteration in the level of the 2.9-kilobase polyadenylated RNA which encodes the Mr = 90,000 yeast heat shock protein is regulated at the level of transcription.
doi_str_mv 10.1016/S0021-9258(18)34346-1
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subjects Bacteriophage lambda - genetics
DNA Restriction Enzymes
DNA, Recombinant - metabolism
Escherichia coli - genetics
Heat-Shock Proteins
Hot Temperature
Nucleic Acid Hybridization
Poly A - genetics
Poly A - isolation & purification
Protein Biosynthesis
Proteins - genetics
RNA - genetics
RNA - isolation & purification
RNA, Messenger
Saccharomyces cerevisiae - genetics
Transcription, Genetic
title Alterations of transcription during heat shock of Saccharomyces cerevisiae
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