Loading…
The dexamethasone-induced inhibitor of fibrinolytic activity in hepatoma cells. A cellular product which specifically inhibits plasminogen activation
Dexamethasone induces an inhibitor of plasminogen-dependent fibrinolysis in rat hepatoma (HTC) cells. The specificity of the inhibitor for urokinase and plasmin was investigated using both fibrinolytic and esterolytic assays. Urokinase, but not plasmin, was inhibited by serum-free conditioned medium...
Saved in:
Published in: | The Journal of biological chemistry 1982-04, Vol.257 (8), p.4260-4264 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c434t-4bd263e1e6bc6d4e0465c1b838c4e0fc202d3fcc5d5ca124e003fb9747ecc2513 |
---|---|
cites | cdi_FETCH-LOGICAL-c434t-4bd263e1e6bc6d4e0465c1b838c4e0fc202d3fcc5d5ca124e003fb9747ecc2513 |
container_end_page | 4264 |
container_issue | 8 |
container_start_page | 4260 |
container_title | The Journal of biological chemistry |
container_volume | 257 |
creator | Coleman, P L Barouski, P A Gelehrter, T D |
description | Dexamethasone induces an inhibitor of plasminogen-dependent fibrinolysis in rat hepatoma (HTC) cells. The specificity of the inhibitor for urokinase and plasmin was investigated using both fibrinolytic and esterolytic assays. Urokinase, but not plasmin, was inhibited by serum-free conditioned medium from cells incubated with 0.1 microM dexamethasone. The specificity of the inhibitor for plasminogen activator was demonstrated directly by the inhibition of the urokinase-catalyzed activation of 125I-plasminogen to 125I-plasmin. The inhibitory activity was stable to pH 3 for 2 h at 37 degrees C, a condition which inactivated fibrinolytic inhibitors in serum, suggesting a cellular origin for the inhibitor. Further evidence for the cellular origin was the constant daily production of inhibitor throughout a 4-day incubation with dexamethasone in serum-free medium. SF HTC-H1 cells, selected for their ability to grow in serum-free medium (Thompson, E. B., Anderson, C. U., and Lippman, M. E. (1975) J. Cell Physiol. 86, 403-412), were grown for 76 days (at least 30 generations) in the presence or absence of serum; dexamethasone induced equivalent amounts of inhibitory activity in cells which had been grown under both conditions. We conclude that the dexamethasone-induced inhibitor from HTC cells is a cellular product which is specific for the inhibition of plasminogen activation and which differs from other reported fibrinolytic inhibitors. |
doi_str_mv | 10.1016/S0021-9258(18)34715-X |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74019133</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S002192581834715X</els_id><sourcerecordid>74019133</sourcerecordid><originalsourceid>FETCH-LOGICAL-c434t-4bd263e1e6bc6d4e0465c1b838c4e0fc202d3fcc5d5ca124e003fb9747ecc2513</originalsourceid><addsrcrecordid>eNqFUctu1DAUtRCoDIVPqGSxQLBIsWPnMStUVbykSiwo0uws5-amuSiJg-20zIfwv3gmQ7d448c95_joHMYupLiUQpbvvwuRy2ybF_VbWb9TupJFtnvCNlLUKlOF3D1lm0fIc_YihJ8iLb2VZ-ys1KUsC71hf2575C3-tiPG3gY3YUZTuwC2nKaeGorOc9fxjhpPkxv2kYBbiHRPcZ8gvMfZRjdaDjgM4ZJfHQ_LYD2fvUtKkT_0BD0PMwJ1BHYY9v-0A58HG8YkfIfTKmsjuekle9bZIeCr037Ofnz6eHv9Jbv59vnr9dVNBlrpmOmmzUuFEssGylaj0GUBsqlVDenSQS7yVnUARVuAlXl6E6prtpWuECAvpDpnb1bdZPXXgiGakcLBv53QLcFUWsitVCoBixUI3oXgsTOzp9H6vZHCHOowxzrMIWsja3Osw-wS7-L0wdKM2D6yTvmn-et13tNd_0AeTUMOehxNXlSmNjovRQJ9WEGYorgn9CYA4ZQ6SgSIpnX0Hxt_AezKqlQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74019133</pqid></control><display><type>article</type><title>The dexamethasone-induced inhibitor of fibrinolytic activity in hepatoma cells. A cellular product which specifically inhibits plasminogen activation</title><source>BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS</source><creator>Coleman, P L ; Barouski, P A ; Gelehrter, T D</creator><creatorcontrib>Coleman, P L ; Barouski, P A ; Gelehrter, T D</creatorcontrib><description>Dexamethasone induces an inhibitor of plasminogen-dependent fibrinolysis in rat hepatoma (HTC) cells. The specificity of the inhibitor for urokinase and plasmin was investigated using both fibrinolytic and esterolytic assays. Urokinase, but not plasmin, was inhibited by serum-free conditioned medium from cells incubated with 0.1 microM dexamethasone. The specificity of the inhibitor for plasminogen activator was demonstrated directly by the inhibition of the urokinase-catalyzed activation of 125I-plasminogen to 125I-plasmin. The inhibitory activity was stable to pH 3 for 2 h at 37 degrees C, a condition which inactivated fibrinolytic inhibitors in serum, suggesting a cellular origin for the inhibitor. Further evidence for the cellular origin was the constant daily production of inhibitor throughout a 4-day incubation with dexamethasone in serum-free medium. SF HTC-H1 cells, selected for their ability to grow in serum-free medium (Thompson, E. B., Anderson, C. U., and Lippman, M. E. (1975) J. Cell Physiol. 86, 403-412), were grown for 76 days (at least 30 generations) in the presence or absence of serum; dexamethasone induced equivalent amounts of inhibitory activity in cells which had been grown under both conditions. We conclude that the dexamethasone-induced inhibitor from HTC cells is a cellular product which is specific for the inhibition of plasminogen activation and which differs from other reported fibrinolytic inhibitors.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)34715-X</identifier><identifier>PMID: 6461654</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Line ; Culture Media ; Dexamethasone - pharmacology ; Fibrinolysin - antagonists & inhibitors ; Fibrinolysis - drug effects ; Kinetics ; Liver Neoplasms, Experimental - physiopathology ; Plasminogen - metabolism ; Rats ; Urokinase-Type Plasminogen Activator - antagonists & inhibitors</subject><ispartof>The Journal of biological chemistry, 1982-04, Vol.257 (8), p.4260-4264</ispartof><rights>1982 © 1982 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-4bd263e1e6bc6d4e0465c1b838c4e0fc202d3fcc5d5ca124e003fb9747ecc2513</citedby><cites>FETCH-LOGICAL-c434t-4bd263e1e6bc6d4e0465c1b838c4e0fc202d3fcc5d5ca124e003fb9747ecc2513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6461654$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coleman, P L</creatorcontrib><creatorcontrib>Barouski, P A</creatorcontrib><creatorcontrib>Gelehrter, T D</creatorcontrib><title>The dexamethasone-induced inhibitor of fibrinolytic activity in hepatoma cells. A cellular product which specifically inhibits plasminogen activation</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Dexamethasone induces an inhibitor of plasminogen-dependent fibrinolysis in rat hepatoma (HTC) cells. The specificity of the inhibitor for urokinase and plasmin was investigated using both fibrinolytic and esterolytic assays. Urokinase, but not plasmin, was inhibited by serum-free conditioned medium from cells incubated with 0.1 microM dexamethasone. The specificity of the inhibitor for plasminogen activator was demonstrated directly by the inhibition of the urokinase-catalyzed activation of 125I-plasminogen to 125I-plasmin. The inhibitory activity was stable to pH 3 for 2 h at 37 degrees C, a condition which inactivated fibrinolytic inhibitors in serum, suggesting a cellular origin for the inhibitor. Further evidence for the cellular origin was the constant daily production of inhibitor throughout a 4-day incubation with dexamethasone in serum-free medium. SF HTC-H1 cells, selected for their ability to grow in serum-free medium (Thompson, E. B., Anderson, C. U., and Lippman, M. E. (1975) J. Cell Physiol. 86, 403-412), were grown for 76 days (at least 30 generations) in the presence or absence of serum; dexamethasone induced equivalent amounts of inhibitory activity in cells which had been grown under both conditions. We conclude that the dexamethasone-induced inhibitor from HTC cells is a cellular product which is specific for the inhibition of plasminogen activation and which differs from other reported fibrinolytic inhibitors.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Culture Media</subject><subject>Dexamethasone - pharmacology</subject><subject>Fibrinolysin - antagonists & inhibitors</subject><subject>Fibrinolysis - drug effects</subject><subject>Kinetics</subject><subject>Liver Neoplasms, Experimental - physiopathology</subject><subject>Plasminogen - metabolism</subject><subject>Rats</subject><subject>Urokinase-Type Plasminogen Activator - antagonists & inhibitors</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><recordid>eNqFUctu1DAUtRCoDIVPqGSxQLBIsWPnMStUVbykSiwo0uws5-amuSiJg-20zIfwv3gmQ7d448c95_joHMYupLiUQpbvvwuRy2ybF_VbWb9TupJFtnvCNlLUKlOF3D1lm0fIc_YihJ8iLb2VZ-ys1KUsC71hf2575C3-tiPG3gY3YUZTuwC2nKaeGorOc9fxjhpPkxv2kYBbiHRPcZ8gvMfZRjdaDjgM4ZJfHQ_LYD2fvUtKkT_0BD0PMwJ1BHYY9v-0A58HG8YkfIfTKmsjuekle9bZIeCr037Ofnz6eHv9Jbv59vnr9dVNBlrpmOmmzUuFEssGylaj0GUBsqlVDenSQS7yVnUARVuAlXl6E6prtpWuECAvpDpnb1bdZPXXgiGakcLBv53QLcFUWsitVCoBixUI3oXgsTOzp9H6vZHCHOowxzrMIWsja3Osw-wS7-L0wdKM2D6yTvmn-et13tNd_0AeTUMOehxNXlSmNjovRQJ9WEGYorgn9CYA4ZQ6SgSIpnX0Hxt_AezKqlQ</recordid><startdate>19820425</startdate><enddate>19820425</enddate><creator>Coleman, P L</creator><creator>Barouski, P A</creator><creator>Gelehrter, T D</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19820425</creationdate><title>The dexamethasone-induced inhibitor of fibrinolytic activity in hepatoma cells. A cellular product which specifically inhibits plasminogen activation</title><author>Coleman, P L ; Barouski, P A ; Gelehrter, T D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-4bd263e1e6bc6d4e0465c1b838c4e0fc202d3fcc5d5ca124e003fb9747ecc2513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Culture Media</topic><topic>Dexamethasone - pharmacology</topic><topic>Fibrinolysin - antagonists & inhibitors</topic><topic>Fibrinolysis - drug effects</topic><topic>Kinetics</topic><topic>Liver Neoplasms, Experimental - physiopathology</topic><topic>Plasminogen - metabolism</topic><topic>Rats</topic><topic>Urokinase-Type Plasminogen Activator - antagonists & inhibitors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coleman, P L</creatorcontrib><creatorcontrib>Barouski, P A</creatorcontrib><creatorcontrib>Gelehrter, T D</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coleman, P L</au><au>Barouski, P A</au><au>Gelehrter, T D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The dexamethasone-induced inhibitor of fibrinolytic activity in hepatoma cells. A cellular product which specifically inhibits plasminogen activation</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1982-04-25</date><risdate>1982</risdate><volume>257</volume><issue>8</issue><spage>4260</spage><epage>4264</epage><pages>4260-4264</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Dexamethasone induces an inhibitor of plasminogen-dependent fibrinolysis in rat hepatoma (HTC) cells. The specificity of the inhibitor for urokinase and plasmin was investigated using both fibrinolytic and esterolytic assays. Urokinase, but not plasmin, was inhibited by serum-free conditioned medium from cells incubated with 0.1 microM dexamethasone. The specificity of the inhibitor for plasminogen activator was demonstrated directly by the inhibition of the urokinase-catalyzed activation of 125I-plasminogen to 125I-plasmin. The inhibitory activity was stable to pH 3 for 2 h at 37 degrees C, a condition which inactivated fibrinolytic inhibitors in serum, suggesting a cellular origin for the inhibitor. Further evidence for the cellular origin was the constant daily production of inhibitor throughout a 4-day incubation with dexamethasone in serum-free medium. SF HTC-H1 cells, selected for their ability to grow in serum-free medium (Thompson, E. B., Anderson, C. U., and Lippman, M. E. (1975) J. Cell Physiol. 86, 403-412), were grown for 76 days (at least 30 generations) in the presence or absence of serum; dexamethasone induced equivalent amounts of inhibitory activity in cells which had been grown under both conditions. We conclude that the dexamethasone-induced inhibitor from HTC cells is a cellular product which is specific for the inhibition of plasminogen activation and which differs from other reported fibrinolytic inhibitors.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6461654</pmid><doi>10.1016/S0021-9258(18)34715-X</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0021-9258 |
ispartof | The Journal of biological chemistry, 1982-04, Vol.257 (8), p.4260-4264 |
issn | 0021-9258 1083-351X |
language | eng |
recordid | cdi_proquest_miscellaneous_74019133 |
source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS |
subjects | Animals Cell Line Culture Media Dexamethasone - pharmacology Fibrinolysin - antagonists & inhibitors Fibrinolysis - drug effects Kinetics Liver Neoplasms, Experimental - physiopathology Plasminogen - metabolism Rats Urokinase-Type Plasminogen Activator - antagonists & inhibitors |
title | The dexamethasone-induced inhibitor of fibrinolytic activity in hepatoma cells. A cellular product which specifically inhibits plasminogen activation |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T21%3A43%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20dexamethasone-induced%20inhibitor%20of%20fibrinolytic%20activity%20in%20hepatoma%20cells.%20A%20cellular%20product%20which%20specifically%20inhibits%20plasminogen%20activation&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Coleman,%20P%20L&rft.date=1982-04-25&rft.volume=257&rft.issue=8&rft.spage=4260&rft.epage=4264&rft.pages=4260-4264&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1016/S0021-9258(18)34715-X&rft_dat=%3Cproquest_cross%3E74019133%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c434t-4bd263e1e6bc6d4e0465c1b838c4e0fc202d3fcc5d5ca124e003fb9747ecc2513%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=74019133&rft_id=info:pmid/6461654&rfr_iscdi=true |