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Identification of a phylogenetically conserved activin-responsive enhancer in the Zic3 gene
Multiple signaling pathways are involved in the induction of the organizer, a major center controlling vertebrate body plan formation. To study these signals, we have focused on the regulation of the Zic3 gene, which codes for a zinc finger transcription factor expressed in the organizer region at t...
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| Published in: | Mechanisms of development 2003-08, Vol.120 (8), p.955-964 |
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| Language: | English |
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| container_title | Mechanisms of development |
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| creator | Weber, Joseph R. Sokol, Sergei Y. |
| description | Multiple signaling pathways are involved in the induction of the organizer, a major center controlling vertebrate body plan formation. To study these signals, we have focused on the regulation of the
Zic3 gene, which codes for a zinc finger transcription factor expressed in the organizer region at the beginning of gastrulation. We searched for DNA regulatory elements in the
Zic3 promoter by testing their ability to drive reporter gene expression in early embryos. By this approach, we identified an
activin
responsive
enhancer (
Zic3-ARE), which was located in the
Zic3 first intron and was essential for dorsal activation of the reporter. The
Zic3-ARE was stimulated by activin and Nodal ligands, but not by a dominant negative bone morphogenetic protein (BMP) receptor. The
Zic3-ARE contains a repeating consensus homeodomain binding sequence, CTAATTAAA, suggesting involvement of a homeodomain transcription factor(s). Mutations in this motif abolished enhancer activity in dorsal marginal zone and its response to activin in animal pole explants. Inhibition of either Wnt/β-catenin or activin/Nodal signaling suppressed
Zic3-ARE activity in dorsal blastomeres, further illustrating the importance of these pathways in activation of organizer genes. |
| doi_str_mv | 10.1016/S0925-4773(03)00082-0 |
| format | article |
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Zic3 gene, which codes for a zinc finger transcription factor expressed in the organizer region at the beginning of gastrulation. We searched for DNA regulatory elements in the
Zic3 promoter by testing their ability to drive reporter gene expression in early embryos. By this approach, we identified an
activin
responsive
enhancer (
Zic3-ARE), which was located in the
Zic3 first intron and was essential for dorsal activation of the reporter. The
Zic3-ARE was stimulated by activin and Nodal ligands, but not by a dominant negative bone morphogenetic protein (BMP) receptor. The
Zic3-ARE contains a repeating consensus homeodomain binding sequence, CTAATTAAA, suggesting involvement of a homeodomain transcription factor(s). Mutations in this motif abolished enhancer activity in dorsal marginal zone and its response to activin in animal pole explants. Inhibition of either Wnt/β-catenin or activin/Nodal signaling suppressed
Zic3-ARE activity in dorsal blastomeres, further illustrating the importance of these pathways in activation of organizer genes.</description><identifier>ISSN: 0925-4773</identifier><identifier>EISSN: 1872-6356</identifier><identifier>DOI: 10.1016/S0925-4773(03)00082-0</identifier><identifier>PMID: 12963115</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Activin ; Activins - genetics ; Activins - metabolism ; Base Sequence ; Conserved Sequence ; Enhancer ; Gene Expression Regulation ; Gene Expression Regulation, Developmental ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; Molecular Sequence Data ; Nodal ; Phylogeny ; Promoter ; Promoter Regions, Genetic ; Signal Transduction ; Smad2 ; Spemann organizer ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic ; Wnt ; Xenopus ; Zic3</subject><ispartof>Mechanisms of development, 2003-08, Vol.120 (8), p.955-964</ispartof><rights>2003 Elsevier Ireland Ltd</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c276t-c14c5b9f1b982fb9855105101766cb39319dbac45436df37cb6b11ae07789e233</citedby><cites>FETCH-LOGICAL-c276t-c14c5b9f1b982fb9855105101766cb39319dbac45436df37cb6b11ae07789e233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12963115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weber, Joseph R.</creatorcontrib><creatorcontrib>Sokol, Sergei Y.</creatorcontrib><title>Identification of a phylogenetically conserved activin-responsive enhancer in the Zic3 gene</title><title>Mechanisms of development</title><addtitle>Mech Dev</addtitle><description>Multiple signaling pathways are involved in the induction of the organizer, a major center controlling vertebrate body plan formation. To study these signals, we have focused on the regulation of the
Zic3 gene, which codes for a zinc finger transcription factor expressed in the organizer region at the beginning of gastrulation. We searched for DNA regulatory elements in the
Zic3 promoter by testing their ability to drive reporter gene expression in early embryos. By this approach, we identified an
activin
responsive
enhancer (
Zic3-ARE), which was located in the
Zic3 first intron and was essential for dorsal activation of the reporter. The
Zic3-ARE was stimulated by activin and Nodal ligands, but not by a dominant negative bone morphogenetic protein (BMP) receptor. The
Zic3-ARE contains a repeating consensus homeodomain binding sequence, CTAATTAAA, suggesting involvement of a homeodomain transcription factor(s). Mutations in this motif abolished enhancer activity in dorsal marginal zone and its response to activin in animal pole explants. Inhibition of either Wnt/β-catenin or activin/Nodal signaling suppressed
Zic3-ARE activity in dorsal blastomeres, further illustrating the importance of these pathways in activation of organizer genes.</description><subject>Activin</subject><subject>Activins - genetics</subject><subject>Activins - metabolism</subject><subject>Base Sequence</subject><subject>Conserved Sequence</subject><subject>Enhancer</subject><subject>Gene Expression Regulation</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Nodal</subject><subject>Phylogeny</subject><subject>Promoter</subject><subject>Promoter Regions, Genetic</subject><subject>Signal Transduction</subject><subject>Smad2</subject><subject>Spemann organizer</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Wnt</subject><subject>Xenopus</subject><subject>Zic3</subject><issn>0925-4773</issn><issn>1872-6356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LAzEQQIMotlZ_gpKT6GE1s9lNuieR4keh4EG96CFks7M2ss3WZFvovzdrix6FYSYMb2bII-QU2BUwENfPrEjzJJOSXzB-yRgbpwnbI0MYyzQRPBf7ZPiLDMhRCJ8RAhBwSAaQFoID5EPyPq3Qdba2Rne2dbStqabL-aZpP9BhF9tNs6GmdQH9GiuqTWfX1iUewzI27Ropurl2Bj21jnZzpG_WcNpPH5ODWjcBT3Z1RF7v714mj8ns6WE6uZ0lJpWiSwxkJi-LGspinNYx5TmwGCCFMCUvOBRVqU2WZ1xUNZemFCWARibluMCU8xE53-5d-vZrhaFTCxsMNo122K6CklxkLMt6MN-CxrcheKzV0tuF9hsFTPVW1Y9V1StTLEZvNT5G5Gx3YFUusPqb2mmMwM0WwPjNtUWvgrEYpVTWo-lU1dp_TnwDYcqHIg</recordid><startdate>200308</startdate><enddate>200308</enddate><creator>Weber, Joseph R.</creator><creator>Sokol, Sergei Y.</creator><general>Elsevier Ireland Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200308</creationdate><title>Identification of a phylogenetically conserved activin-responsive enhancer in the Zic3 gene</title><author>Weber, Joseph R. ; Sokol, Sergei Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c276t-c14c5b9f1b982fb9855105101766cb39319dbac45436df37cb6b11ae07789e233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Activin</topic><topic>Activins - genetics</topic><topic>Activins - metabolism</topic><topic>Base Sequence</topic><topic>Conserved Sequence</topic><topic>Enhancer</topic><topic>Gene Expression Regulation</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Nodal</topic><topic>Phylogeny</topic><topic>Promoter</topic><topic>Promoter Regions, Genetic</topic><topic>Signal Transduction</topic><topic>Smad2</topic><topic>Spemann organizer</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Wnt</topic><topic>Xenopus</topic><topic>Zic3</topic><toplevel>online_resources</toplevel><creatorcontrib>Weber, Joseph R.</creatorcontrib><creatorcontrib>Sokol, Sergei Y.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mechanisms of development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weber, Joseph R.</au><au>Sokol, Sergei Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a phylogenetically conserved activin-responsive enhancer in the Zic3 gene</atitle><jtitle>Mechanisms of development</jtitle><addtitle>Mech Dev</addtitle><date>2003-08</date><risdate>2003</risdate><volume>120</volume><issue>8</issue><spage>955</spage><epage>964</epage><pages>955-964</pages><issn>0925-4773</issn><eissn>1872-6356</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Multiple signaling pathways are involved in the induction of the organizer, a major center controlling vertebrate body plan formation. To study these signals, we have focused on the regulation of the
Zic3 gene, which codes for a zinc finger transcription factor expressed in the organizer region at the beginning of gastrulation. We searched for DNA regulatory elements in the
Zic3 promoter by testing their ability to drive reporter gene expression in early embryos. By this approach, we identified an
activin
responsive
enhancer (
Zic3-ARE), which was located in the
Zic3 first intron and was essential for dorsal activation of the reporter. The
Zic3-ARE was stimulated by activin and Nodal ligands, but not by a dominant negative bone morphogenetic protein (BMP) receptor. The
Zic3-ARE contains a repeating consensus homeodomain binding sequence, CTAATTAAA, suggesting involvement of a homeodomain transcription factor(s). Mutations in this motif abolished enhancer activity in dorsal marginal zone and its response to activin in animal pole explants. Inhibition of either Wnt/β-catenin or activin/Nodal signaling suppressed
Zic3-ARE activity in dorsal blastomeres, further illustrating the importance of these pathways in activation of organizer genes.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>12963115</pmid><doi>10.1016/S0925-4773(03)00082-0</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Mechanisms of development, 2003-08, Vol.120 (8), p.955-964 |
| issn | 0925-4773 1872-6356 |
| language | eng |
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| source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS; ScienceDirect Journals |
| subjects | Activin Activins - genetics Activins - metabolism Base Sequence Conserved Sequence Enhancer Gene Expression Regulation Gene Expression Regulation, Developmental Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Molecular Sequence Data Nodal Phylogeny Promoter Promoter Regions, Genetic Signal Transduction Smad2 Spemann organizer Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Wnt Xenopus Zic3 |
| title | Identification of a phylogenetically conserved activin-responsive enhancer in the Zic3 gene |
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