Impaired generation of taurine chloramine by synovial fluid neutrophils of rheumatoid arthritis patients

Taurine (Tau), a dominant free amino acid present in neutrophil cytoplasm, serves as a scavenger for hypochlorous acid (HOCl) released during these cells activation. The resulting taurine chloramine (Tau-Cl) exerts potent anti-inflammatory properties. In the present study we tested the hypothesis th...

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Bibliographic Details
Published in:Amino acids 2002, Vol.23 (4), p.415-418
Main Authors: Kontny, E, Wojtecka-ŁUkasik, E, Rell-Bakalarska, K, Dziewczopolski, W, Maśliński, W, Maślinski, S
Format: Article
Language:English
Subjects:
Adult
Aged
Arthritis, Rheumatoid - immunology
Arthritis, Rheumatoid - metabolism
Female
Humans
Luminescent Measurements
Male
Middle Aged
Neutrophils - metabolism
Respiratory Burst
Synovial Fluid - cytology
Taurine - analogs & derivatives
Taurine - metabolism
Zymosan - metabolism
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Summary:Taurine (Tau), a dominant free amino acid present in neutrophil cytoplasm, serves as a scavenger for hypochlorous acid (HOCl) released during these cells activation. The resulting taurine chloramine (Tau-Cl) exerts potent anti-inflammatory properties. In the present study we tested the hypothesis that the formation of Tau-Cl is impaired in neutrophils isolated from rheumatoid arthritis (RA) patients. The inhibition of zymosan-triggered chemiluminescence in the presence of exogenous Tau was used for indirect measurement of Tau-Cl generation. The chemiluminescence of neutrophils isolated from peripheral blood (PB) of healthy volunteers and RA patients was inhibited by Tau with similar potency. By contrast, synovial fluid (SF) neutrophils of these patients were significantly less sensitive for Tau-mediated inhibition. Therefore, our data indicate impaired generation of Tau-Cl in neutrophils isolated from SF of RA patients.
ISSN:0939-4451
1438-2199
DOI:10.1007/s00726-002-0205-z