Similar genotypes of Pneumocystis jirovecii in different forms of Pneumocystis infection

1 Service de Parasitologie, Mycologie et Médecine des Voyages, Hôpital Sud, Centre Hospitalier Universitaire, Université de Picardie Jules Verne, 1 rue Laennec, 80054 Amiens, Paris, France 2 Centre Hospitalier Universitaire Saint Antoine, Paris, France Correspondence Anne Totet totet.anne{at}chu-ami...

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Published in:Microbiology (Society for General Microbiology) 2004-05, Vol.150 (5), p.1173-1178
Main Authors: Totet, Anne, Duwat, Hyacinthe, Magois, Eline, Jounieaux, Vincent, Roux, Patricia, Raccurt, Christian, Nevez, Gilles
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Bronchiolitis - microbiology
Bronchoalveolar Lavage Fluid - microbiology
Dihydropteroate Synthase - genetics
DNA, Fungal - analysis
DNA, Ribosomal Spacer - genetics
Female
Genetic Variation
Genotype
Humans
Infant
Male
Middle Aged
Molecular Sequence Data
Pneumocystis carinii - classification
Pneumocystis carinii - genetics
Pneumocystis carinii - isolation & purification
Pneumocystis Infections - microbiology
Pneumocystis jiroveci
Pneumonia, Pneumocystis - microbiology
Polymerase Chain Reaction
Retrospective Studies
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Summary:1 Service de Parasitologie, Mycologie et Médecine des Voyages, Hôpital Sud, Centre Hospitalier Universitaire, Université de Picardie Jules Verne, 1 rue Laennec, 80054 Amiens, Paris, France 2 Centre Hospitalier Universitaire Saint Antoine, Paris, France Correspondence Anne Totet totet.anne{at}chu-amiens.fr This study describes the genotyping of Pneumocystis jirovecii organisms isolated from three groups of patients that developed diverse forms of P. jirovecii infection; the patients were monitored in the same French hospital. Forty archival specimens from 13 adults with Pneumocystis pneumonia, eight adults colonized by P. jirovecii and 19 immunocompetent infants infected with the fungus contemporaneously with a bronchiolitis episode were analysed retrospectively. Genotyping was performed by analysis of sequences of the internal transcribed spacer (ITS)1 and ITS2 regions, and of the dihydropteroate synthase (DHPS) locus. At the ITS regions, a high diversity of genotypes, identical main genotypes (B 1 a 3 and B 2 a 1 ) and the occurrence of mixed infections (more than one genotype) were observed in the three patient groups. At the DHPS locus, the results indicated the presence of mutants in the two adult groups, as well as in the infant group. Consequently, at these two independent genomic regions, P. jirovecii isolates from patients who developed different forms of infection and who lived in the same geographical region presented common characteristics. These results suggest that patients infected with P. jirovecii , whatever the form of infection they present, are part of a common human reservoir for P. jirovecii . Abbreviations: DHPS, dihydropteroate synthase; ITS, internal transcribed spacer; PCP, Pneumocystis pneumonia
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.26919-0