Design and synthesis of germline-based hemi-humanized single-chain Fv against the CD18 surface antigen

The 6.7 murine monoclonal antibody (mAb) recognizes the human CD18 antigen and is therefore of interest as an anti-inflammatory agent. The 6.7 heavy variable chain (VH) was humanized using the closest human germline sequence as the template on to which to graft the murine complementary determining r...

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Published in:Protein engineering 2000-05, Vol.13 (5), p.353-360
Main Authors: Caldas, Cristina, Coelho, Verônica P.C.V., Rigden, Daniel J., Neschich, Goran, Moro, Ana Maria, Brígido, Marcelo M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
CD18
CD18 Antigens - immunology
Cloning, Molecular
humanization
Humans
Immunoglobulin Variable Region - chemistry
Immunoglobulin Variable Region - genetics
Immunoglobulin Variable Region - immunology
Mice
Models, Molecular
Molecular Sequence Data
monoclonal antibody
Pichia - genetics
Pichia pastoris
scFv
Sequence Homology, Amino Acid
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cited_by cdi_FETCH-LOGICAL-c427t-fed959714927043a3bdac52aaba80ee33f3adda08aa09ae53442e218fe03afe93
cites cdi_FETCH-LOGICAL-c427t-fed959714927043a3bdac52aaba80ee33f3adda08aa09ae53442e218fe03afe93
container_end_page 360
container_issue 5
container_start_page 353
container_title Protein engineering
container_volume 13
creator Caldas, Cristina
Coelho, Verônica P.C.V.
Rigden, Daniel J.
Neschich, Goran
Moro, Ana Maria
Brígido, Marcelo M.
description The 6.7 murine monoclonal antibody (mAb) recognizes the human CD18 antigen and is therefore of interest as an anti-inflammatory agent. The 6.7 heavy variable chain (VH) was humanized using the closest human germline sequence as the template on to which to graft the murine complementary determining regions (CDRs). Two versions were proposed, one in which the residue proline 45 of the murine form was maintained and another in which this framework residue was changed to the leucine found in the human sequence. These VH humanized versions were expressed in the yeast Pichia pastoris as hemi-humanized single-chain Fv (scFvs), with the VL from the murine antibody. The scFv from the murine antibody was also expressed. The binding activities of the murine and both hemi-humanized scFvs were determined by flow cytometry analysis. All the constructions were able to recognize human lymphocytes harboring CD18, indicating successful humanization with transfer of the original binding capability. Some differences between the two hemi-humanized versions were observed. The method used was simple and straightforward, with no need for refined structural analyses and could be used for the humanization of other antibodies.
doi_str_mv 10.1093/protein/13.5.353
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1460-213X
1741-0134
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source Oxford University Press Journals
subjects Amino Acid Sequence
Animals
CD18
CD18 Antigens - immunology
Cloning, Molecular
humanization
Humans
Immunoglobulin Variable Region - chemistry
Immunoglobulin Variable Region - genetics
Immunoglobulin Variable Region - immunology
Mice
Models, Molecular
Molecular Sequence Data
monoclonal antibody
Pichia - genetics
Pichia pastoris
scFv
Sequence Homology, Amino Acid
title Design and synthesis of germline-based hemi-humanized single-chain Fv against the CD18 surface antigen
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