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Cholesterol-dependent clustering of IL-2Ralpha and its colocalization with HLA and CD48 on T lymphoma cells suggest their functional association with lipid rafts
Immunogold staining and electron microscopy show that IL-2 receptor alpha-subunits exhibit nonrandom surface distribution on human T lymphoma cells. Analysis of interparticle distances reveals that this clustering on the scale of a few hundred nanometers is independent of the presence of IL-2 and of...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 2000-05, Vol.97 (11), p.6013-6018 |
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| creator | Vereb, G Matkó, J Vámosi, G Ibrahim, S M Magyar, E Varga, S Szöllosi, J Jenei, A Gáspár, Jr, R Waldmann, T A Damjanovich, S |
| description | Immunogold staining and electron microscopy show that IL-2 receptor alpha-subunits exhibit nonrandom surface distribution on human T lymphoma cells. Analysis of interparticle distances reveals that this clustering on the scale of a few hundred nanometers is independent of the presence of IL-2 and of the expression of the IL-2R beta-subunit. Clustering of IL-2Ralpha is confirmed by confocal microscopy, yielding the same average cluster size, approximately 600-800 nm, as electron microscopy. HLA class I and II and CD48 molecules also form clusters of the same size. Disruption of cholesterol-rich lipid rafts with filipin or depletion of membrane cholesterol with methyl-beta-cyclodextrin results in the blurring of cluster boundaries and an apparent dispersion of clusters for all four proteins. Interestingly, the transferrin receptor, which is thought to be located outside lipid rafts, exhibits clusters that are only 300 nm in size and are less affected by modifying the membrane cholesterol content. Furthermore, transferrin receptor clusters hardly colocalize with IL-2Ralpha, HLA, and CD48 molecules (crosscorrelation coefficient is 0.05), whereas IL-2Ralpha colocalizes with both HLA and CD48 (crosscorrelation coefficient is between 0.37 and 0.46). This coclustering is confirmed by electron microscopy. The submicron clusters of IL-2Ralpha chains and their coclustering with HLA and CD48, presumably associated with lipid rafts, could underlie the efficiency of signaling in lymphoid cells. |
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Analysis of interparticle distances reveals that this clustering on the scale of a few hundred nanometers is independent of the presence of IL-2 and of the expression of the IL-2R beta-subunit. Clustering of IL-2Ralpha is confirmed by confocal microscopy, yielding the same average cluster size, approximately 600-800 nm, as electron microscopy. HLA class I and II and CD48 molecules also form clusters of the same size. Disruption of cholesterol-rich lipid rafts with filipin or depletion of membrane cholesterol with methyl-beta-cyclodextrin results in the blurring of cluster boundaries and an apparent dispersion of clusters for all four proteins. Interestingly, the transferrin receptor, which is thought to be located outside lipid rafts, exhibits clusters that are only 300 nm in size and are less affected by modifying the membrane cholesterol content. Furthermore, transferrin receptor clusters hardly colocalize with IL-2Ralpha, HLA, and CD48 molecules (crosscorrelation coefficient is 0.05), whereas IL-2Ralpha colocalizes with both HLA and CD48 (crosscorrelation coefficient is between 0.37 and 0.46). This coclustering is confirmed by electron microscopy. The submicron clusters of IL-2Ralpha chains and their coclustering with HLA and CD48, presumably associated with lipid rafts, could underlie the efficiency of signaling in lymphoid cells.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>PMID: 10823948</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Antigens, CD - analysis ; CD48 Antigen ; Cholesterol ; Cholesterol - physiology ; Cytokines ; HLA Antigens - analysis ; Humans ; Immunohistochemistry ; Immunology ; Lipids ; Lymphoma, T-Cell - pathology ; Membrane Fluidity ; Membrane Lipids - physiology ; Microscopy, Confocal ; Microscopy, Immunoelectron ; Neoplasm Proteins - analysis ; Receptors, Interleukin-2 - analysis ; T-Lymphocytes - metabolism ; Tumor Cells, Cultured</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2000-05, Vol.97 (11), p.6013-6018</ispartof><rights>Copyright National Academy of Sciences May 23, 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10823948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vereb, G</creatorcontrib><creatorcontrib>Matkó, J</creatorcontrib><creatorcontrib>Vámosi, G</creatorcontrib><creatorcontrib>Ibrahim, S M</creatorcontrib><creatorcontrib>Magyar, E</creatorcontrib><creatorcontrib>Varga, S</creatorcontrib><creatorcontrib>Szöllosi, J</creatorcontrib><creatorcontrib>Jenei, A</creatorcontrib><creatorcontrib>Gáspár, Jr, R</creatorcontrib><creatorcontrib>Waldmann, T A</creatorcontrib><creatorcontrib>Damjanovich, S</creatorcontrib><title>Cholesterol-dependent clustering of IL-2Ralpha and its colocalization with HLA and CD48 on T lymphoma cells suggest their functional association with lipid rafts</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Immunogold staining and electron microscopy show that IL-2 receptor alpha-subunits exhibit nonrandom surface distribution on human T lymphoma cells. 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Furthermore, transferrin receptor clusters hardly colocalize with IL-2Ralpha, HLA, and CD48 molecules (crosscorrelation coefficient is 0.05), whereas IL-2Ralpha colocalizes with both HLA and CD48 (crosscorrelation coefficient is between 0.37 and 0.46). This coclustering is confirmed by electron microscopy. The submicron clusters of IL-2Ralpha chains and their coclustering with HLA and CD48, presumably associated with lipid rafts, could underlie the efficiency of signaling in lymphoid cells.</description><subject>Antigens, CD - analysis</subject><subject>CD48 Antigen</subject><subject>Cholesterol</subject><subject>Cholesterol - physiology</subject><subject>Cytokines</subject><subject>HLA Antigens - analysis</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunology</subject><subject>Lipids</subject><subject>Lymphoma, T-Cell - pathology</subject><subject>Membrane Fluidity</subject><subject>Membrane Lipids - physiology</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Immunoelectron</subject><subject>Neoplasm Proteins - analysis</subject><subject>Receptors, Interleukin-2 - analysis</subject><subject>T-Lymphocytes - metabolism</subject><subject>Tumor Cells, Cultured</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpdkN1Kw0AQhRdRbK2-gixeeBfY3ST7c1nqTwsBQXofNptJs2WTjdkEqW_jm5pqBfFq4Mw3Z87MGZpTomjEE0XO0ZwQJiKZsGSGrkLYE0JUKsklmlEiWawSOUefq9o7CAP03kUldNCW0A7YuPGo2XaHfYU3WcRetetqjXVbYjsEbLzzRjv7oQfrW_xuhxqvs-V3f_WQSDyJW-wOTVf7RmMDzgUcxt1u2oWHGmyPq7E1x2HtsA7BG_vHytnOlrjX1RCu0UWlXYCbU12g7dPjdrWOspfnzWqZRZ2kMlJa8MoAA2EM5yJRXIlEMGIoJIaawqiCq7RiVaoAUkYUB6UqIGC4KCiIeIHuf2y73r-NU8q8seEYW7fgx5ALSpkgSk3g3T9w78d-uiLkjNA4pbGUE3R7gsaigTLvetvo_pD_Pj7-AtqlgiE</recordid><startdate>20000523</startdate><enddate>20000523</enddate><creator>Vereb, G</creator><creator>Matkó, J</creator><creator>Vámosi, G</creator><creator>Ibrahim, S M</creator><creator>Magyar, E</creator><creator>Varga, S</creator><creator>Szöllosi, J</creator><creator>Jenei, A</creator><creator>Gáspár, Jr, R</creator><creator>Waldmann, T A</creator><creator>Damjanovich, S</creator><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000523</creationdate><title>Cholesterol-dependent clustering of IL-2Ralpha and its colocalization with HLA and CD48 on T lymphoma cells suggest their functional association with lipid rafts</title><author>Vereb, G ; Matkó, J ; Vámosi, G ; Ibrahim, S M ; Magyar, E ; Varga, S ; Szöllosi, J ; Jenei, A ; Gáspár, Jr, R ; Waldmann, T A ; Damjanovich, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p818-9a76fce2e7cc667496974720c1e4c1cbc9b695f2f59ee52096e99fe0ec67b1e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Antigens, CD - analysis</topic><topic>CD48 Antigen</topic><topic>Cholesterol</topic><topic>Cholesterol - physiology</topic><topic>Cytokines</topic><topic>HLA Antigens - analysis</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunology</topic><topic>Lipids</topic><topic>Lymphoma, T-Cell - pathology</topic><topic>Membrane Fluidity</topic><topic>Membrane Lipids - physiology</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Immunoelectron</topic><topic>Neoplasm Proteins - analysis</topic><topic>Receptors, Interleukin-2 - analysis</topic><topic>T-Lymphocytes - metabolism</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vereb, G</creatorcontrib><creatorcontrib>Matkó, J</creatorcontrib><creatorcontrib>Vámosi, G</creatorcontrib><creatorcontrib>Ibrahim, S M</creatorcontrib><creatorcontrib>Magyar, E</creatorcontrib><creatorcontrib>Varga, S</creatorcontrib><creatorcontrib>Szöllosi, J</creatorcontrib><creatorcontrib>Jenei, A</creatorcontrib><creatorcontrib>Gáspár, Jr, R</creatorcontrib><creatorcontrib>Waldmann, T A</creatorcontrib><creatorcontrib>Damjanovich, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vereb, G</au><au>Matkó, J</au><au>Vámosi, G</au><au>Ibrahim, S M</au><au>Magyar, E</au><au>Varga, S</au><au>Szöllosi, J</au><au>Jenei, A</au><au>Gáspár, Jr, R</au><au>Waldmann, T A</au><au>Damjanovich, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cholesterol-dependent clustering of IL-2Ralpha and its colocalization with HLA and CD48 on T lymphoma cells suggest their functional association with lipid rafts</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2000-05-23</date><risdate>2000</risdate><volume>97</volume><issue>11</issue><spage>6013</spage><epage>6018</epage><pages>6013-6018</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Immunogold staining and electron microscopy show that IL-2 receptor alpha-subunits exhibit nonrandom surface distribution on human T lymphoma cells. Analysis of interparticle distances reveals that this clustering on the scale of a few hundred nanometers is independent of the presence of IL-2 and of the expression of the IL-2R beta-subunit. Clustering of IL-2Ralpha is confirmed by confocal microscopy, yielding the same average cluster size, approximately 600-800 nm, as electron microscopy. HLA class I and II and CD48 molecules also form clusters of the same size. Disruption of cholesterol-rich lipid rafts with filipin or depletion of membrane cholesterol with methyl-beta-cyclodextrin results in the blurring of cluster boundaries and an apparent dispersion of clusters for all four proteins. Interestingly, the transferrin receptor, which is thought to be located outside lipid rafts, exhibits clusters that are only 300 nm in size and are less affected by modifying the membrane cholesterol content. Furthermore, transferrin receptor clusters hardly colocalize with IL-2Ralpha, HLA, and CD48 molecules (crosscorrelation coefficient is 0.05), whereas IL-2Ralpha colocalizes with both HLA and CD48 (crosscorrelation coefficient is between 0.37 and 0.46). This coclustering is confirmed by electron microscopy. The submicron clusters of IL-2Ralpha chains and their coclustering with HLA and CD48, presumably associated with lipid rafts, could underlie the efficiency of signaling in lymphoid cells.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>10823948</pmid><tpages>6</tpages></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2000-05, Vol.97 (11), p.6013-6018 |
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| source | Open Access: PubMed Central; JSTOR Archival Journals and Primary Sources Collection |
| subjects | Antigens, CD - analysis CD48 Antigen Cholesterol Cholesterol - physiology Cytokines HLA Antigens - analysis Humans Immunohistochemistry Immunology Lipids Lymphoma, T-Cell - pathology Membrane Fluidity Membrane Lipids - physiology Microscopy, Confocal Microscopy, Immunoelectron Neoplasm Proteins - analysis Receptors, Interleukin-2 - analysis T-Lymphocytes - metabolism Tumor Cells, Cultured |
| title | Cholesterol-dependent clustering of IL-2Ralpha and its colocalization with HLA and CD48 on T lymphoma cells suggest their functional association with lipid rafts |
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