IGF-1 Regulates Apoptosis of Cardiac Myocyte Induced by Osmotic-Stress

Insulin-like growth factor-1 (IGF-1) is a natural protectant of cardiac myocytes that has been shown to improve cardiac function. The role of IGF-1 in attenuating apoptosis induced by osmotic stress (sorbitol, SOR) or by other known apoptotic stimuli (doxorubicin, angiotensin II, and serum withdrawa...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-04, Vol.270 (3), p.1029-1035
Main Authors: Morales, María Paz, Gálvez, Anita, Eltit, José Miguel, Ocaranza, Paula, Díaz-Araya, Guillermo, Lavandero, Sergio
Format: Article
Language:English
Subjects:
angiotensin
Angiotensin II - pharmacology
Animals
Animals, Newborn
apoptosis
Apoptosis - drug effects
Apoptosis - physiology
Cell Survival - drug effects
Cells, Cultured
Culture Media, Serum-Free
doxorubicin
Doxorubicin - pharmacology
Heart - drug effects
Heart Ventricles
Insulin-Like Growth Factor I - pharmacology
insulin-like growth factor-1
Kinetics
Myocardium - cytology
Osmolar Concentration
osmotic stress
poly(ADP-ribose) polymerase
Rats
Rats, Sprague-Dawley
Sorbitol - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Insulin-like growth factor-1 (IGF-1) is a natural protectant of cardiac myocytes that has been shown to improve cardiac function. The role of IGF-1 in attenuating apoptosis induced by osmotic stress (sorbitol, SOR) or by other known apoptotic stimuli (doxorubicin, angiotensin II, and serum withdrawal) was determined in cultured cardiac myocytes. After 6 h of exposure to SOR, apoptosis was initiated, concomitant with a decrease in cell survival and increases in poly-[ADP-ribose] polymerase (PARP) degradation and DNA fragmentation. These effects were maximal after 24 h. IGF-1 partially attenuated apoptosis induced by sorbitol but not that induced by angiotensin II, doxorubicin, or serum withdrawal. In cells preincubated with IGF-1 before the addition of SOR, we detected an increase in the number of viable cells, a decrease in the generation of DNA fragments on agarose gel electrophoresis and in the percentage of positive TUNEL cells, and a reduction on PARP levels. These results suggest that IGF-1 prevents apoptosis induced by osmotic stress in cardiac myocytes but not apoptosis induced by doxorubicin and angiotensin II.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.2550