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High-efficiency endovascular gene delivery via therapeutic ultrasound
OBJECTIVES We studied enhancement of local gene delivery to the arterial wall by using an endovascular catheter ultrasound (US). BACKGROUND Ultrasound exposure is standard for enhancement of in vitro gene delivery. We postulate that in vivo endovascular applications can be safely developed. METHODS...
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| Published in: | Journal of the American College of Cardiology 2001-06, Vol.37 (7), p.1975-1980 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c425t-61548dcca00f113704a418c32c4c4b90499dc00acd7d467503a73f0a5aa1e2553 |
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| cites | cdi_FETCH-LOGICAL-c425t-61548dcca00f113704a418c32c4c4b90499dc00acd7d467503a73f0a5aa1e2553 |
| container_end_page | 1980 |
| container_issue | 7 |
| container_start_page | 1975 |
| container_title | Journal of the American College of Cardiology |
| container_volume | 37 |
| creator | Amabile, Philippe G Waugh, Jacob M Lewis, Thomas N Elkins, Christopher J Janas, Wolfgang Dake, Michael D |
| description | OBJECTIVES
We studied enhancement of local gene delivery to the arterial wall by using an endovascular catheter ultrasound (US).
BACKGROUND
Ultrasound exposure is standard for enhancement of in vitro gene delivery. We postulate that in vivo endovascular applications can be safely developed.
METHODS
We used a rabbit model of arterial mechanical overdilation injury. After arterial overdilation, US catheters were introduced in bilateral rabbit femoral arteries and perfused with plasmid- or adenovirus-expressing blue fluorescent protein (BFP) or phosphate buffered saline. One side received endovascular US (2 MHz, 50 W/cm2, 16 min), and the contralateral artery did not.
RESULTS
Relative to controls, US exposure enhanced BFP expression measured via fluorescence 12-fold for plasmid (1,502.1 ± 927.3 vs. 18,053.9 ± 11,612 μm2, p < 0.05) and 19-fold for adenovirus (877.1 ± 577.7 vs. 17,213.15 ± 3,892 μm2, p < 0.05) while increasing cell death for the adenovirus group only (26 ± 5.78% vs. 13 ± 2.55%, p < 0.012).
CONCLUSIONS
Endovascular US enhanced vascular gene delivery and increased the efficiency of nonviral platforms to levels previously attained only by adenoviral strategies. |
| doi_str_mv | 10.1016/S0735-1097(01)01253-0 |
| format | article |
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We studied enhancement of local gene delivery to the arterial wall by using an endovascular catheter ultrasound (US).
BACKGROUND
Ultrasound exposure is standard for enhancement of in vitro gene delivery. We postulate that in vivo endovascular applications can be safely developed.
METHODS
We used a rabbit model of arterial mechanical overdilation injury. After arterial overdilation, US catheters were introduced in bilateral rabbit femoral arteries and perfused with plasmid- or adenovirus-expressing blue fluorescent protein (BFP) or phosphate buffered saline. One side received endovascular US (2 MHz, 50 W/cm2, 16 min), and the contralateral artery did not.
RESULTS
Relative to controls, US exposure enhanced BFP expression measured via fluorescence 12-fold for plasmid (1,502.1 ± 927.3 vs. 18,053.9 ± 11,612 μm2, p < 0.05) and 19-fold for adenovirus (877.1 ± 577.7 vs. 17,213.15 ± 3,892 μm2, p < 0.05) while increasing cell death for the adenovirus group only (26 ± 5.78% vs. 13 ± 2.55%, p < 0.012).
CONCLUSIONS
Endovascular US enhanced vascular gene delivery and increased the efficiency of nonviral platforms to levels previously attained only by adenoviral strategies.</description><identifier>ISSN: 0735-1097</identifier><identifier>EISSN: 1558-3597</identifier><identifier>DOI: 10.1016/S0735-1097(01)01253-0</identifier><identifier>PMID: 11401141</identifier><identifier>CODEN: JACCDI</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Angioscopy ; Animals ; Arteries ; Biological and medical sciences ; Diseases of the cardiovascular system ; Genetic Therapy - methods ; Male ; Medical sciences ; Rabbits ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Ultrasonography, Interventional</subject><ispartof>Journal of the American College of Cardiology, 2001-06, Vol.37 (7), p.1975-1980</ispartof><rights>2001 American College of Cardiology</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-61548dcca00f113704a418c32c4c4b90499dc00acd7d467503a73f0a5aa1e2553</citedby><cites>FETCH-LOGICAL-c425t-61548dcca00f113704a418c32c4c4b90499dc00acd7d467503a73f0a5aa1e2553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1058453$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11401141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amabile, Philippe G</creatorcontrib><creatorcontrib>Waugh, Jacob M</creatorcontrib><creatorcontrib>Lewis, Thomas N</creatorcontrib><creatorcontrib>Elkins, Christopher J</creatorcontrib><creatorcontrib>Janas, Wolfgang</creatorcontrib><creatorcontrib>Dake, Michael D</creatorcontrib><title>High-efficiency endovascular gene delivery via therapeutic ultrasound</title><title>Journal of the American College of Cardiology</title><addtitle>J Am Coll Cardiol</addtitle><description>OBJECTIVES
We studied enhancement of local gene delivery to the arterial wall by using an endovascular catheter ultrasound (US).
BACKGROUND
Ultrasound exposure is standard for enhancement of in vitro gene delivery. We postulate that in vivo endovascular applications can be safely developed.
METHODS
We used a rabbit model of arterial mechanical overdilation injury. After arterial overdilation, US catheters were introduced in bilateral rabbit femoral arteries and perfused with plasmid- or adenovirus-expressing blue fluorescent protein (BFP) or phosphate buffered saline. One side received endovascular US (2 MHz, 50 W/cm2, 16 min), and the contralateral artery did not.
RESULTS
Relative to controls, US exposure enhanced BFP expression measured via fluorescence 12-fold for plasmid (1,502.1 ± 927.3 vs. 18,053.9 ± 11,612 μm2, p < 0.05) and 19-fold for adenovirus (877.1 ± 577.7 vs. 17,213.15 ± 3,892 μm2, p < 0.05) while increasing cell death for the adenovirus group only (26 ± 5.78% vs. 13 ± 2.55%, p < 0.012).
CONCLUSIONS
Endovascular US enhanced vascular gene delivery and increased the efficiency of nonviral platforms to levels previously attained only by adenoviral strategies.</description><subject>Angioscopy</subject><subject>Animals</subject><subject>Arteries</subject><subject>Biological and medical sciences</subject><subject>Diseases of the cardiovascular system</subject><subject>Genetic Therapy - methods</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Rabbits</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Ultrasonography, Interventional</subject><issn>0735-1097</issn><issn>1558-3597</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LxDAQhoMoun78BKUHET1UZ5pk255ExC8QPKjnMCZTjXTbNWkX9t_b_UC9eRjm8rzvDI8QhwjnCDi-eIZc6hShzE8BzwAzLVPYECPUukilLvNNMfpBdsRujJ8AMC6w3BY7iAqGwZG4uffvHylXlbeeGztPuHHtjKLtawrJOzecOK79jMM8mXlKug8ONOW-8zbp6y5QbPvG7YutiurIB-u9J15vb16u79PHp7uH66vH1KpMd-kYtSqctQRQIcocFCksrMyssuqtBFWWzgKQdblT41yDpFxWQJoIOdNa7omTVe80tF89x85MfLRc19Rw20eTQ5lJWcoB1CvQhjbGwJWZBj-hMDcIZuHPLP2ZhRwDaJb-DAy5o_WB_m3C7je1FjYAx2tgcER1FaixPv5p14XSi_uXK4wHGzPPwcSlX3Y-sO2Ma_0_n3wD0BiLyg</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Amabile, Philippe G</creator><creator>Waugh, Jacob M</creator><creator>Lewis, Thomas N</creator><creator>Elkins, Christopher J</creator><creator>Janas, Wolfgang</creator><creator>Dake, Michael D</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>High-efficiency endovascular gene delivery via therapeutic ultrasound</title><author>Amabile, Philippe G ; Waugh, Jacob M ; Lewis, Thomas N ; Elkins, Christopher J ; Janas, Wolfgang ; Dake, Michael D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-61548dcca00f113704a418c32c4c4b90499dc00acd7d467503a73f0a5aa1e2553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Angioscopy</topic><topic>Animals</topic><topic>Arteries</topic><topic>Biological and medical sciences</topic><topic>Diseases of the cardiovascular system</topic><topic>Genetic Therapy - methods</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Rabbits</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Ultrasonography, Interventional</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amabile, Philippe G</creatorcontrib><creatorcontrib>Waugh, Jacob M</creatorcontrib><creatorcontrib>Lewis, Thomas N</creatorcontrib><creatorcontrib>Elkins, Christopher J</creatorcontrib><creatorcontrib>Janas, Wolfgang</creatorcontrib><creatorcontrib>Dake, Michael D</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American College of Cardiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amabile, Philippe G</au><au>Waugh, Jacob M</au><au>Lewis, Thomas N</au><au>Elkins, Christopher J</au><au>Janas, Wolfgang</au><au>Dake, Michael D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-efficiency endovascular gene delivery via therapeutic ultrasound</atitle><jtitle>Journal of the American College of Cardiology</jtitle><addtitle>J Am Coll Cardiol</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>37</volume><issue>7</issue><spage>1975</spage><epage>1980</epage><pages>1975-1980</pages><issn>0735-1097</issn><eissn>1558-3597</eissn><coden>JACCDI</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>OBJECTIVES
We studied enhancement of local gene delivery to the arterial wall by using an endovascular catheter ultrasound (US).
BACKGROUND
Ultrasound exposure is standard for enhancement of in vitro gene delivery. We postulate that in vivo endovascular applications can be safely developed.
METHODS
We used a rabbit model of arterial mechanical overdilation injury. After arterial overdilation, US catheters were introduced in bilateral rabbit femoral arteries and perfused with plasmid- or adenovirus-expressing blue fluorescent protein (BFP) or phosphate buffered saline. One side received endovascular US (2 MHz, 50 W/cm2, 16 min), and the contralateral artery did not.
RESULTS
Relative to controls, US exposure enhanced BFP expression measured via fluorescence 12-fold for plasmid (1,502.1 ± 927.3 vs. 18,053.9 ± 11,612 μm2, p < 0.05) and 19-fold for adenovirus (877.1 ± 577.7 vs. 17,213.15 ± 3,892 μm2, p < 0.05) while increasing cell death for the adenovirus group only (26 ± 5.78% vs. 13 ± 2.55%, p < 0.012).
CONCLUSIONS
Endovascular US enhanced vascular gene delivery and increased the efficiency of nonviral platforms to levels previously attained only by adenoviral strategies.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11401141</pmid><doi>10.1016/S0735-1097(01)01253-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of the American College of Cardiology, 2001-06, Vol.37 (7), p.1975-1980 |
| issn | 0735-1097 1558-3597 |
| language | eng |
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| source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS |
| subjects | Angioscopy Animals Arteries Biological and medical sciences Diseases of the cardiovascular system Genetic Therapy - methods Male Medical sciences Rabbits Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Ultrasonography, Interventional |
| title | High-efficiency endovascular gene delivery via therapeutic ultrasound |
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