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Contrasting Behavior of Zwitterionic and Cationic Polymers Bound to Anionic Liposomes
Zwitterionic polymers were prepared by quaternizing polyvinylpyridine (DP = 1100) with bromoacids (Br(CH2) n COOH, where n = 1, 2, 3, and 5). The resulting polymers were then added to unilamellar liposomes composed of egg lecithin or dipalmitoylphosphatidylcholine admixed with 20 mol % of cardiolipi...
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| Published in: | Langmuir 2007-07, Vol.23 (14), p.7539-7544 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a417t-c1967fc2cd0153ff2e0019082bb296952f2108ab24eb5766a5c7f9a7f5ba27103 |
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| cites | cdi_FETCH-LOGICAL-a417t-c1967fc2cd0153ff2e0019082bb296952f2108ab24eb5766a5c7f9a7f5ba27103 |
| container_end_page | 7544 |
| container_issue | 14 |
| container_start_page | 7539 |
| container_title | Langmuir |
| container_volume | 23 |
| creator | Yaroslavov, A. A Sitnikova, T. A Rakhnyanskaya, A. A Ermakov, Yu. A Burova, T. V Grinberg, V. Ya Menger, F. M |
| description | Zwitterionic polymers were prepared by quaternizing polyvinylpyridine (DP = 1100) with bromoacids (Br(CH2) n COOH, where n = 1, 2, 3, and 5). The resulting polymers were then added to unilamellar liposomes composed of egg lecithin or dipalmitoylphosphatidylcholine admixed with 20 mol % of cardiolipin (a phospholipid with two negative charges). These systems were compared (along with polyethylvinylpyridinium chloride, a polycation) by light scattering, electrophoretic mobility, fluorescence, and high-sensitivity differential scanning calorimetry. The external zwitterionic polymers induce no flip-flop of cardiolipin from the inner leaflet to the outer leaflet as does the polycation. Aside from this similarity, the four zwitterionic polymers all behave differently from each other toward the anionic liposomes: (a) For n = 1, there is no detectable interaction between the polymer and the liposomes. (b) For n = 2, electrostatic attraction induces polymer−liposome association (reversed by the addition of NaCl) that maintains the original negative charge on the liposome. Aggregation of the liposomes accompanies polymer adsorption. (c) For n = 3, electrostatic binding also occurs along with aggregation. However, the binding is so strong that NaCl is unable to induce polymer/liposome dissociation. (d) For n = 5, there is polymer binding and NaCl-promoted dissociation but no substantial aggregation. These differences among the closely related polymers are discussed and analyzed in molecular terms. |
| doi_str_mv | 10.1021/la700637d |
| format | article |
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A ; Sitnikova, T. A ; Rakhnyanskaya, A. A ; Ermakov, Yu. A ; Burova, T. V ; Grinberg, V. Ya ; Menger, F. M</creator><creatorcontrib>Yaroslavov, A. A ; Sitnikova, T. A ; Rakhnyanskaya, A. A ; Ermakov, Yu. A ; Burova, T. V ; Grinberg, V. Ya ; Menger, F. M</creatorcontrib><description>Zwitterionic polymers were prepared by quaternizing polyvinylpyridine (DP = 1100) with bromoacids (Br(CH2) n COOH, where n = 1, 2, 3, and 5). The resulting polymers were then added to unilamellar liposomes composed of egg lecithin or dipalmitoylphosphatidylcholine admixed with 20 mol % of cardiolipin (a phospholipid with two negative charges). These systems were compared (along with polyethylvinylpyridinium chloride, a polycation) by light scattering, electrophoretic mobility, fluorescence, and high-sensitivity differential scanning calorimetry. The external zwitterionic polymers induce no flip-flop of cardiolipin from the inner leaflet to the outer leaflet as does the polycation. Aside from this similarity, the four zwitterionic polymers all behave differently from each other toward the anionic liposomes: (a) For n = 1, there is no detectable interaction between the polymer and the liposomes. (b) For n = 2, electrostatic attraction induces polymer−liposome association (reversed by the addition of NaCl) that maintains the original negative charge on the liposome. Aggregation of the liposomes accompanies polymer adsorption. (c) For n = 3, electrostatic binding also occurs along with aggregation. However, the binding is so strong that NaCl is unable to induce polymer/liposome dissociation. (d) For n = 5, there is polymer binding and NaCl-promoted dissociation but no substantial aggregation. These differences among the closely related polymers are discussed and analyzed in molecular terms.</description><identifier>ISSN: 0743-7463</identifier><identifier>EISSN: 1520-5827</identifier><identifier>DOI: 10.1021/la700637d</identifier><identifier>PMID: 17550275</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>1,2-Dipalmitoylphosphatidylcholine - chemistry ; Anions ; Calorimetry, Differential Scanning ; Carboxylic Acids - chemistry ; Cardiolipins - chemistry ; Cations ; Electrophoresis ; Fluorescence ; Hydrocarbons, Brominated - chemistry ; Liposomes - chemistry ; Phosphatidylcholines - chemistry ; Polymers - chemistry ; Polyvinyls - chemistry ; Pyridines - chemistry ; Scattering, Radiation ; Sodium Chloride - chemistry ; Static Electricity ; Surface Properties ; Surface-Active Agents - chemistry</subject><ispartof>Langmuir, 2007-07, Vol.23 (14), p.7539-7544</ispartof><rights>Copyright © 2007 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a417t-c1967fc2cd0153ff2e0019082bb296952f2108ab24eb5766a5c7f9a7f5ba27103</citedby><cites>FETCH-LOGICAL-a417t-c1967fc2cd0153ff2e0019082bb296952f2108ab24eb5766a5c7f9a7f5ba27103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17550275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yaroslavov, A. 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These systems were compared (along with polyethylvinylpyridinium chloride, a polycation) by light scattering, electrophoretic mobility, fluorescence, and high-sensitivity differential scanning calorimetry. The external zwitterionic polymers induce no flip-flop of cardiolipin from the inner leaflet to the outer leaflet as does the polycation. Aside from this similarity, the four zwitterionic polymers all behave differently from each other toward the anionic liposomes: (a) For n = 1, there is no detectable interaction between the polymer and the liposomes. (b) For n = 2, electrostatic attraction induces polymer−liposome association (reversed by the addition of NaCl) that maintains the original negative charge on the liposome. Aggregation of the liposomes accompanies polymer adsorption. (c) For n = 3, electrostatic binding also occurs along with aggregation. However, the binding is so strong that NaCl is unable to induce polymer/liposome dissociation. (d) For n = 5, there is polymer binding and NaCl-promoted dissociation but no substantial aggregation. These differences among the closely related polymers are discussed and analyzed in molecular terms.</description><subject>1,2-Dipalmitoylphosphatidylcholine - chemistry</subject><subject>Anions</subject><subject>Calorimetry, Differential Scanning</subject><subject>Carboxylic Acids - chemistry</subject><subject>Cardiolipins - chemistry</subject><subject>Cations</subject><subject>Electrophoresis</subject><subject>Fluorescence</subject><subject>Hydrocarbons, Brominated - chemistry</subject><subject>Liposomes - chemistry</subject><subject>Phosphatidylcholines - chemistry</subject><subject>Polymers - chemistry</subject><subject>Polyvinyls - chemistry</subject><subject>Pyridines - chemistry</subject><subject>Scattering, Radiation</subject><subject>Sodium Chloride - chemistry</subject><subject>Static Electricity</subject><subject>Surface Properties</subject><subject>Surface-Active Agents - chemistry</subject><issn>0743-7463</issn><issn>1520-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNptkE1LxDAQhoMouq4e_APSi4KH6iRtku3RLX7hgiurFy8hbRONts2apOr-eytd9CJzGGbehxl4EDrAcIqB4LNacgCW8GoDjTAlENMJ4ZtoBDxNYp6yZAftev8KAFmSZttoB3NKgXA6Qo-5bYOTPpj2OZqqF_lhrIusjp4-TQjKGduaMpJtFeUyDMPc1qtGOR9Nbdfvg43O2yGZmaX1tlF-D21pWXu1v-5j9Hh58ZBfx7O7q5v8fBbLFPMQlzhjXJekrADTRGuiAHAGE1IUJGMZJZpgmMiCpKqgnDFJS64zyTUtJOEYkjE6Hu4unX3vlA-iMb5UdS1bZTsvODDaV9KDJwNYOuu9U1osnWmkWwkM4seh-HXYs4fro13RqOqPXEvrgXgAjA_q6zeX7k0wnnAqHuYLcU_Y1e31IhWznj8aeFl68Wo71_ZO_nn8DZIHhwk</recordid><startdate>20070703</startdate><enddate>20070703</enddate><creator>Yaroslavov, A. A</creator><creator>Sitnikova, T. A</creator><creator>Rakhnyanskaya, A. A</creator><creator>Ermakov, Yu. A</creator><creator>Burova, T. V</creator><creator>Grinberg, V. Ya</creator><creator>Menger, F. M</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070703</creationdate><title>Contrasting Behavior of Zwitterionic and Cationic Polymers Bound to Anionic Liposomes</title><author>Yaroslavov, A. A ; Sitnikova, T. A ; Rakhnyanskaya, A. A ; Ermakov, Yu. A ; Burova, T. V ; Grinberg, V. Ya ; Menger, F. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a417t-c1967fc2cd0153ff2e0019082bb296952f2108ab24eb5766a5c7f9a7f5ba27103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>1,2-Dipalmitoylphosphatidylcholine - chemistry</topic><topic>Anions</topic><topic>Calorimetry, Differential Scanning</topic><topic>Carboxylic Acids - chemistry</topic><topic>Cardiolipins - chemistry</topic><topic>Cations</topic><topic>Electrophoresis</topic><topic>Fluorescence</topic><topic>Hydrocarbons, Brominated - chemistry</topic><topic>Liposomes - chemistry</topic><topic>Phosphatidylcholines - chemistry</topic><topic>Polymers - chemistry</topic><topic>Polyvinyls - chemistry</topic><topic>Pyridines - chemistry</topic><topic>Scattering, Radiation</topic><topic>Sodium Chloride - chemistry</topic><topic>Static Electricity</topic><topic>Surface Properties</topic><topic>Surface-Active Agents - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yaroslavov, A. A</creatorcontrib><creatorcontrib>Sitnikova, T. A</creatorcontrib><creatorcontrib>Rakhnyanskaya, A. A</creatorcontrib><creatorcontrib>Ermakov, Yu. A</creatorcontrib><creatorcontrib>Burova, T. V</creatorcontrib><creatorcontrib>Grinberg, V. Ya</creatorcontrib><creatorcontrib>Menger, F. M</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Langmuir</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yaroslavov, A. A</au><au>Sitnikova, T. A</au><au>Rakhnyanskaya, A. A</au><au>Ermakov, Yu. A</au><au>Burova, T. V</au><au>Grinberg, V. Ya</au><au>Menger, F. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contrasting Behavior of Zwitterionic and Cationic Polymers Bound to Anionic Liposomes</atitle><jtitle>Langmuir</jtitle><addtitle>Langmuir</addtitle><date>2007-07-03</date><risdate>2007</risdate><volume>23</volume><issue>14</issue><spage>7539</spage><epage>7544</epage><pages>7539-7544</pages><issn>0743-7463</issn><eissn>1520-5827</eissn><notes>istex:6495A819EF28ED4DD9E2F437B362B891F5316DBC</notes><notes>ark:/67375/TPS-Q26GKHS4-L</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Zwitterionic polymers were prepared by quaternizing polyvinylpyridine (DP = 1100) with bromoacids (Br(CH2) n COOH, where n = 1, 2, 3, and 5). The resulting polymers were then added to unilamellar liposomes composed of egg lecithin or dipalmitoylphosphatidylcholine admixed with 20 mol % of cardiolipin (a phospholipid with two negative charges). These systems were compared (along with polyethylvinylpyridinium chloride, a polycation) by light scattering, electrophoretic mobility, fluorescence, and high-sensitivity differential scanning calorimetry. The external zwitterionic polymers induce no flip-flop of cardiolipin from the inner leaflet to the outer leaflet as does the polycation. Aside from this similarity, the four zwitterionic polymers all behave differently from each other toward the anionic liposomes: (a) For n = 1, there is no detectable interaction between the polymer and the liposomes. (b) For n = 2, electrostatic attraction induces polymer−liposome association (reversed by the addition of NaCl) that maintains the original negative charge on the liposome. Aggregation of the liposomes accompanies polymer adsorption. (c) For n = 3, electrostatic binding also occurs along with aggregation. However, the binding is so strong that NaCl is unable to induce polymer/liposome dissociation. (d) For n = 5, there is polymer binding and NaCl-promoted dissociation but no substantial aggregation. These differences among the closely related polymers are discussed and analyzed in molecular terms.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>17550275</pmid><doi>10.1021/la700637d</doi><tpages>6</tpages></addata></record> |
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| ispartof | Langmuir, 2007-07, Vol.23 (14), p.7539-7544 |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | 1,2-Dipalmitoylphosphatidylcholine - chemistry Anions Calorimetry, Differential Scanning Carboxylic Acids - chemistry Cardiolipins - chemistry Cations Electrophoresis Fluorescence Hydrocarbons, Brominated - chemistry Liposomes - chemistry Phosphatidylcholines - chemistry Polymers - chemistry Polyvinyls - chemistry Pyridines - chemistry Scattering, Radiation Sodium Chloride - chemistry Static Electricity Surface Properties Surface-Active Agents - chemistry |
| title | Contrasting Behavior of Zwitterionic and Cationic Polymers Bound to Anionic Liposomes |
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