A simple, sensitive and widely applicable ELISA for S100B: Methodological features of the measurement of this glial protein

S100B expression, particularly extracellular S100B, is used as a parameter of glial activation and/or death in several situations of brain injury. Several immunoassays for S100B measurement are available, which differ with regard to specificity, sensitivity, sample application, and, of course, econo...

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Published in:Journal of neuroscience methods 2008-03, Vol.169 (1), p.93-99
Main Authors: Leite, Marina Concli, Galland, Fabiana, Brolese, Giovana, Guerra, Maria Cristina, Bortolotto, Josiane Woutheres, Freitas, Rodrigo, Almeida, Lucia Maria Vieira de, Gottfried, Carmem, Gonçalves, Carlos-Alberto
Format: Article
Language:English
Subjects:
Adipocyte
Adipose Tissue - metabolism
Adult
Animals
Astrocyte
Astrocytes - metabolism
Brain - metabolism
Brain Chemistry - physiology
Brain injury
Cell Line, Tumor
Cells, Cultured
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Female
Humans
Infant, Newborn
Male
Nerve Growth Factors - analysis
Nerve Growth Factors - blood
Nerve Growth Factors - cerebrospinal fluid
Neurochemistry - methods
Neuroglia - chemistry
Neuroglia - metabolism
Oxidation-Reduction
Oxidative Stress
Rats
Rats, Wistar
S100 Calcium Binding Protein beta Subunit
S100 Proteins - analysis
S100 Proteins - blood
S100 Proteins - cerebrospinal fluid
S100B protein
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Summary:S100B expression, particularly extracellular S100B, is used as a parameter of glial activation and/or death in several situations of brain injury. Several immunoassays for S100B measurement are available, which differ with regard to specificity, sensitivity, sample application, and, of course, economic costs. We standardized two protocols for S100B measurement (range between 1.9 pg and 10 ng/mL) in human and rat samples from brain and adipose tissues, blood serum, cerebrospinal fluid, urine and cell culture. Abundance and secretion of this protein in adipose tissue reinforces the caution about its origin in blood serum. Interestingly, S100B recognition was affected by the redox status of the protein. This aspect should be considered in S100B measurement, assuming that oxidized and reduced forms possibly coexist in vivo and the equilibrium can be modified by oxidative stress of physiological or pathological conditions or even by obtaining sample conditions.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2007.11.021