Beneficial Effects of Phosphatidylcholine During Hindlimb Reperfusion

Background Microcirculatory dysfunctions and mast cell (MC) reactions play important roles in hypoxic tissue injuries. The aims of this study were to characterize the effects of hindlimb ischemia-reperfusion (I-R) on the periosteal microcirculation and to define the consequences of systemic phosphat...

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Published in:The Journal of surgical research 2007-05, Vol.139 (1), p.45-50
Main Authors: Gera, László, M.D, Varga, Renáta, M.D, Török, László, M.D, Kaszaki, József, Ph.D, Szabó, Andrea, M.D., Ph.D, Nagy, Katalin, M.D., Ph.D, Boros, Mihály, M.D., Ph.D
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Degranulation
General aspects
Hindlimb - blood supply
Inflammation - prevention & control
leukocyte
Male
mast cell
Mast Cells - physiology
Medical sciences
Microcirculation
periosteum
Peroxidase - metabolism
Phosphatidylcholines - therapeutic use
rat
Rats
Rats, Wistar
Reperfusion Injury - prevention & control
Surgery
videomicroscopy
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Summary:Background Microcirculatory dysfunctions and mast cell (MC) reactions play important roles in hypoxic tissue injuries. The aims of this study were to characterize the effects of hindlimb ischemia-reperfusion (I-R) on the periosteal microcirculation and to define the consequences of systemic phosphatidylcholine (PC) therapy during this condition. Materials and methods Microcirculatory changes were visualized by means of fluorescence intravital videomicroscopy in anesthetized Wistar rats. There was 60 min of complete hindlimb ischemia followed by a 180-min reperfusion in the presence of PC treatment (50 mg/kg i.v.; in the second 10 min of reperfusion) or vehicle. Further two groups served as vehicle- or PC-treated sham-operated controls. The proportion of degranulated MCs and the leukocyte accumulation (myeloperoxidase, MPO assay) were determined in muscle biopsies. Results I-R significantly increased the muscle MPO activity (from 14.94 to 63.45 mU/mg) and the proportion of degranulated MCs (to 82.5%). The periosteal capillary RBC velocity (RBCV) and the functional capillary density (FCD) had decreased, while the primary and secondary leukocyte-endothelial cell interactions had increased by the end of reperfusion (rolling from 20.8 to 40.0%, and firm adherence from 254 to 872 mm−2 ). PC treatment decreased the leukocyte rolling and sticking, preserved the FCD and improved the RBCV. The MC degranulation and MPO activity diminished significantly in the muscle layer. Conclusions PC administration improves I-R-induced periosteal microcirculatory dysfunctions and ameliorates secondary inflammatory reactions. Systemic PC treatment could offer a potential treatment modality during hypoperfusion or inflammatory conditions of the bones.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2006.08.004