IDENTIFICATION OF LIGANDS BINDING SPECIFICALLY TO INFLAMMATORY INTESTINAL MUCOSA USING PHAGE DISPLAY

SUMMARY 1 Even though current treatments for inflammatory bowel disease are effective, adverse reactions remain a problem. With the intention of developing a new drug delivery system, we attempted to identify molecules that are selectively adsorbed to inflamed bowel. 2 The PhD‐C7C phage display pept...

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Bibliographic Details
Published in:Clinical and experimental pharmacology & physiology 2007-04, Vol.34 (4), p.286-289
Main Authors: Takagi, Tamaki, Arisawa, Tomiyasu, Yamamoto, Kazumasa, Hirata, Ichiro, Nakano, Hiroshi, Sawada, Makoto
Format: Article
Language:English
Subjects:
Adsorption
Amino Acid Sequence
Animals
Bacteriophages - genetics
Binding Sites
Binding, Competitive
inflammatory bowel
Inflammatory Bowel Diseases - genetics
Inflammatory Bowel Diseases - metabolism
Inflammatory Bowel Diseases - pathology
Intestinal Mucosa - metabolism
Intestinal Mucosa - pathology
Ligands
Male
Mice
Mice, Inbred C57BL
Oligopeptides - chemistry
Oligopeptides - genetics
Oligopeptides - metabolism
Peptide Library
phage display
PhD-C7C phage
Reperfusion Injury - physiopathology
targeting ligands
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Summary:SUMMARY 1 Even though current treatments for inflammatory bowel disease are effective, adverse reactions remain a problem. With the intention of developing a new drug delivery system, we attempted to identify molecules that are selectively adsorbed to inflamed bowel. 2 The PhD‐C7C phage display peptide library was used for biopanning against mouse isolated bowel, either untreated (control) or with inflammation caused by ischaemia–reperfusion injury. One hundred clones were selected from among those obtained by two biopanning procedures and the amino acid sequences of these clones were identified by determination of the base sequences. 3 Then, 20 clones were selected by an alignment process, after which the three clones with the highest affinity for inflammatory bowel were identified. One of these three clones had significantly higher affinity for inflammatory bowel than for normal bowel. 4 In conclusion, biopanning against isolated bowel samples identified an amino acid sequence (SQSHPRH) with a specific high affinity for inflammatory bowel.
ISSN:0305-1870
1440-1681
DOI:10.1111/j.1440-1681.2007.04563.x