Identification by a multiplex PCR-based assay of Salmonella typhimurium and Salmonella enteritidis strains from environmental swabs of poultry houses

A multiplex‐PCR‐based assay (m‐PCR) was developed for the detection of Salmonella and for the identification of the two serotypes Enteritidis and Typhimurium. Three sets of primers selected from different genomic sequences amplified a 429 bp fragment specific for the genus Salmonella within a random...

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Bibliographic Details
Published in:Letters in applied microbiology 1999-07, Vol.29 (1), p.1-6
Main Authors: Soumet, C, Ermel, G, Rose, V, Rose, N, Drouin, P, Salvat, G, Colin, P
Format: Article
Language:English
Subjects:
Animal productions
Animals
Bacterial Proteins - genetics
Bacterial Typing Techniques
Bacteriological Techniques
Biological and medical sciences
Chickens
DNA Primers
Environmental Microbiology
Fimbriae Proteins
Flagellin - genetics
Fundamental and applied biological sciences. Psychology
Housing, Animal
microbial contamination
polymerase chain reaction
Polymerase Chain Reaction - methods
Salmonella enteritidis
Salmonella enteritidis - classification
Salmonella enteritidis - genetics
Salmonella enteritidis - isolation & purification
Salmonella typhimurium
Salmonella typhimurium - classification
Salmonella typhimurium - genetics
Salmonella typhimurium - isolation & purification
Sensitivity and Specificity
Terrestrial animal productions
Vertebrates
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Summary:A multiplex‐PCR‐based assay (m‐PCR) was developed for the detection of Salmonella and for the identification of the two serotypes Enteritidis and Typhimurium. Three sets of primers selected from different genomic sequences amplified a 429 bp fragment specific for the genus Salmonella within a randomly cloned sequence, a 559 bp target specific for Salmonella Typhimurium within the fliC gene and a 312 bp fragment specific for Salmonella Enteritidis within the sefA gene. The m‐PCR‐based assay was used for detecting Salmonella from 1078 environmental swabs of poultry houses. Prior to PCR, these swabs were pre‐enriched in phosphate‐buffered peptone water for 18–20 h and then sub‐cultured on a Modified Semi‐solid Rappaport Vassiliadis medium (MSRV) for 18–20 h. The m‐PCR combined with MSRV had a better sensitivity (95%) than the bacteriological method (92·5%). The MSRV‐m‐PCR assay and the bacteriological method had an agreement rate of 95·6%.
ISSN:0266-8254
1472-765X
DOI:10.1046/j.1365-2672.1999.00559.x