Loading…
Increased adipogenicity of cells from regenerating skeletal muscle
Adipose tissue development is observed in some muscle pathologies, however, mechanisms that induce accumulation of this tissue as well as its cellular origin are unknown. The adipogenicity of cells from bupivacaine hydrochloride (BPVC)-treated and untreated muscle was compared in vitro. Culturing ce...
Saved in:
| Published in: | Experimental cell research 2006-09, Vol.312 (15), p.2701-2711 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c450t-74033eb60031ad2a8681f7d08cdfc58f89d3e1e8a83c5077a65a06c9244942ba3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c450t-74033eb60031ad2a8681f7d08cdfc58f89d3e1e8a83c5077a65a06c9244942ba3 |
| container_end_page | 2711 |
| container_issue | 15 |
| container_start_page | 2701 |
| container_title | Experimental cell research |
| container_volume | 312 |
| creator | Yamanouchi, Keitaro Yada, Erica Ishiguro, Naomi Hosoyama, Tohru Nishihara, Masugi |
| description | Adipose tissue development is observed in some muscle pathologies, however, mechanisms that induce accumulation of this tissue as well as its cellular origin are unknown. The adipogenicity of cells from bupivacaine hydrochloride (BPVC)-treated and untreated muscle was compared in vitro. Culturing cells from both BPVC-treated and untreated muscles in adipogenic differentiation medium (ADM) for 10 days resulted in the appearance of mature adipocytes, but their number was 3.5-fold higher in cells from BPVC-treated muscle. Temporal expressions of PPARγ and the presence of lipid droplets during adipogenic differentiation were examined. On day 2 of culture in ADM, only cells from BPVC-treated muscle were positive both for PPARγ and lipid droplets. Pref-1 was expressed in cells from untreated muscle, whereas its expression was absent in cells from BPVC-treated muscle. In ADM, the presence of insulin, which negates an inhibitory effect of Pref-1 on adipogenic differentiation, was required for PPARγ2 expression in cells from untreated muscle, but not for cells from BPVC-treated muscle. These results indicate that BPVC-induced degenerative/regenerative changes in muscle lead to increased adipogenicity of cells, and suggest that this increased adipogenicity not only involves an increase in the number of cells having adipogenic potential, but also contributes to the progression of these cells toward adipogenic differentiation. |
| doi_str_mv | 10.1016/j.yexcr.2006.04.014 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68791310</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014482706001704</els_id><sourcerecordid>1173288801</sourcerecordid><originalsourceid>FETCH-LOGICAL-c450t-74033eb60031ad2a8681f7d08cdfc58f89d3e1e8a83c5077a65a06c9244942ba3</originalsourceid><addsrcrecordid>eNp9kE1rGzEQhkVoSBwnv6BQlh5y2-3oYyXtoYcmJG0gkEtyFrI0a-TuhyvtlvjfR44NhR5yGph5ZublIeQzhYoCld821Q5fXawYgKxAVEDFCVlQaKBkgrFPZAG5VQrN1Dm5SGkDAFpTeUbOqVQ10IYuyM3D4CLahL6wPmzHNQ7BhWlXjG3hsOtS0caxLyLmAUY7hWFdpN_Y4WS7op-T6_CSnLa2S3h1rEvycn_3fPurfHz6-XD747F0ooapVAI4x5UE4NR6ZrXUtFUetPOtq3WrG8-RoraauxqUsrK2IF3DhGgEW1m-JNeHu9s4_pkxTaYPaZ_RDjjOyUitGsopZPDrf-BmnOOQsxnaCKmYkjxD_AC5OKYUsTXbGHobd4aC2fs1G_Pu1-z9GhAmy8xbX46n51WP_t_OUWgGvh8AzCb-BowmuYCDQx8iusn4MXz44A3Bg4wS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>194672763</pqid></control><display><type>article</type><title>Increased adipogenicity of cells from regenerating skeletal muscle</title><source>ScienceDirect Freedom Collection</source><creator>Yamanouchi, Keitaro ; Yada, Erica ; Ishiguro, Naomi ; Hosoyama, Tohru ; Nishihara, Masugi</creator><creatorcontrib>Yamanouchi, Keitaro ; Yada, Erica ; Ishiguro, Naomi ; Hosoyama, Tohru ; Nishihara, Masugi</creatorcontrib><description>Adipose tissue development is observed in some muscle pathologies, however, mechanisms that induce accumulation of this tissue as well as its cellular origin are unknown. The adipogenicity of cells from bupivacaine hydrochloride (BPVC)-treated and untreated muscle was compared in vitro. Culturing cells from both BPVC-treated and untreated muscles in adipogenic differentiation medium (ADM) for 10 days resulted in the appearance of mature adipocytes, but their number was 3.5-fold higher in cells from BPVC-treated muscle. Temporal expressions of PPARγ and the presence of lipid droplets during adipogenic differentiation were examined. On day 2 of culture in ADM, only cells from BPVC-treated muscle were positive both for PPARγ and lipid droplets. Pref-1 was expressed in cells from untreated muscle, whereas its expression was absent in cells from BPVC-treated muscle. In ADM, the presence of insulin, which negates an inhibitory effect of Pref-1 on adipogenic differentiation, was required for PPARγ2 expression in cells from untreated muscle, but not for cells from BPVC-treated muscle. These results indicate that BPVC-induced degenerative/regenerative changes in muscle lead to increased adipogenicity of cells, and suggest that this increased adipogenicity not only involves an increase in the number of cells having adipogenic potential, but also contributes to the progression of these cells toward adipogenic differentiation.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2006.04.014</identifier><identifier>PMID: 16750191</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipocytes ; Adipocytes - cytology ; Adipocytes - drug effects ; Adipocytes - metabolism ; Adipogenesis ; Adipogenesis - drug effects ; Adipose tissue development ; Animals ; Bupivacaine - metabolism ; Bupivacaine - pharmacology ; CCAAT-Enhancer-Binding Protein-alpha - metabolism ; Cell Differentiation ; Cells ; Cells, Cultured ; Gene expression ; Immunohistochemistry ; Insulin - metabolism ; Male ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - physiology ; Muscular system ; MyoD Protein - metabolism ; PPAR gamma - metabolism ; Progenitor cells ; Rats ; Rats, Wistar ; Regeneration - drug effects ; Skeletal muscle ; Tissues</subject><ispartof>Experimental cell research, 2006-09, Vol.312 (15), p.2701-2711</ispartof><rights>2006 Elsevier Inc.</rights><rights>Copyright © 2006 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-74033eb60031ad2a8681f7d08cdfc58f89d3e1e8a83c5077a65a06c9244942ba3</citedby><cites>FETCH-LOGICAL-c450t-74033eb60031ad2a8681f7d08cdfc58f89d3e1e8a83c5077a65a06c9244942ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16750191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamanouchi, Keitaro</creatorcontrib><creatorcontrib>Yada, Erica</creatorcontrib><creatorcontrib>Ishiguro, Naomi</creatorcontrib><creatorcontrib>Hosoyama, Tohru</creatorcontrib><creatorcontrib>Nishihara, Masugi</creatorcontrib><title>Increased adipogenicity of cells from regenerating skeletal muscle</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Adipose tissue development is observed in some muscle pathologies, however, mechanisms that induce accumulation of this tissue as well as its cellular origin are unknown. The adipogenicity of cells from bupivacaine hydrochloride (BPVC)-treated and untreated muscle was compared in vitro. Culturing cells from both BPVC-treated and untreated muscles in adipogenic differentiation medium (ADM) for 10 days resulted in the appearance of mature adipocytes, but their number was 3.5-fold higher in cells from BPVC-treated muscle. Temporal expressions of PPARγ and the presence of lipid droplets during adipogenic differentiation were examined. On day 2 of culture in ADM, only cells from BPVC-treated muscle were positive both for PPARγ and lipid droplets. Pref-1 was expressed in cells from untreated muscle, whereas its expression was absent in cells from BPVC-treated muscle. In ADM, the presence of insulin, which negates an inhibitory effect of Pref-1 on adipogenic differentiation, was required for PPARγ2 expression in cells from untreated muscle, but not for cells from BPVC-treated muscle. These results indicate that BPVC-induced degenerative/regenerative changes in muscle lead to increased adipogenicity of cells, and suggest that this increased adipogenicity not only involves an increase in the number of cells having adipogenic potential, but also contributes to the progression of these cells toward adipogenic differentiation.</description><subject>Adipocytes</subject><subject>Adipocytes - cytology</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - metabolism</subject><subject>Adipogenesis</subject><subject>Adipogenesis - drug effects</subject><subject>Adipose tissue development</subject><subject>Animals</subject><subject>Bupivacaine - metabolism</subject><subject>Bupivacaine - pharmacology</subject><subject>CCAAT-Enhancer-Binding Protein-alpha - metabolism</subject><subject>Cell Differentiation</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Gene expression</subject><subject>Immunohistochemistry</subject><subject>Insulin - metabolism</subject><subject>Male</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - physiology</subject><subject>Muscular system</subject><subject>MyoD Protein - metabolism</subject><subject>PPAR gamma - metabolism</subject><subject>Progenitor cells</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Regeneration - drug effects</subject><subject>Skeletal muscle</subject><subject>Tissues</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kE1rGzEQhkVoSBwnv6BQlh5y2-3oYyXtoYcmJG0gkEtyFrI0a-TuhyvtlvjfR44NhR5yGph5ZublIeQzhYoCld821Q5fXawYgKxAVEDFCVlQaKBkgrFPZAG5VQrN1Dm5SGkDAFpTeUbOqVQ10IYuyM3D4CLahL6wPmzHNQ7BhWlXjG3hsOtS0caxLyLmAUY7hWFdpN_Y4WS7op-T6_CSnLa2S3h1rEvycn_3fPurfHz6-XD747F0ooapVAI4x5UE4NR6ZrXUtFUetPOtq3WrG8-RoraauxqUsrK2IF3DhGgEW1m-JNeHu9s4_pkxTaYPaZ_RDjjOyUitGsopZPDrf-BmnOOQsxnaCKmYkjxD_AC5OKYUsTXbGHobd4aC2fs1G_Pu1-z9GhAmy8xbX46n51WP_t_OUWgGvh8AzCb-BowmuYCDQx8iusn4MXz44A3Bg4wS</recordid><startdate>20060910</startdate><enddate>20060910</enddate><creator>Yamanouchi, Keitaro</creator><creator>Yada, Erica</creator><creator>Ishiguro, Naomi</creator><creator>Hosoyama, Tohru</creator><creator>Nishihara, Masugi</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060910</creationdate><title>Increased adipogenicity of cells from regenerating skeletal muscle</title><author>Yamanouchi, Keitaro ; Yada, Erica ; Ishiguro, Naomi ; Hosoyama, Tohru ; Nishihara, Masugi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-74033eb60031ad2a8681f7d08cdfc58f89d3e1e8a83c5077a65a06c9244942ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adipocytes</topic><topic>Adipocytes - cytology</topic><topic>Adipocytes - drug effects</topic><topic>Adipocytes - metabolism</topic><topic>Adipogenesis</topic><topic>Adipogenesis - drug effects</topic><topic>Adipose tissue development</topic><topic>Animals</topic><topic>Bupivacaine - metabolism</topic><topic>Bupivacaine - pharmacology</topic><topic>CCAAT-Enhancer-Binding Protein-alpha - metabolism</topic><topic>Cell Differentiation</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Gene expression</topic><topic>Immunohistochemistry</topic><topic>Insulin - metabolism</topic><topic>Male</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - physiology</topic><topic>Muscular system</topic><topic>MyoD Protein - metabolism</topic><topic>PPAR gamma - metabolism</topic><topic>Progenitor cells</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Regeneration - drug effects</topic><topic>Skeletal muscle</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamanouchi, Keitaro</creatorcontrib><creatorcontrib>Yada, Erica</creatorcontrib><creatorcontrib>Ishiguro, Naomi</creatorcontrib><creatorcontrib>Hosoyama, Tohru</creatorcontrib><creatorcontrib>Nishihara, Masugi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamanouchi, Keitaro</au><au>Yada, Erica</au><au>Ishiguro, Naomi</au><au>Hosoyama, Tohru</au><au>Nishihara, Masugi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased adipogenicity of cells from regenerating skeletal muscle</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2006-09-10</date><risdate>2006</risdate><volume>312</volume><issue>15</issue><spage>2701</spage><epage>2711</epage><pages>2701-2711</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Adipose tissue development is observed in some muscle pathologies, however, mechanisms that induce accumulation of this tissue as well as its cellular origin are unknown. The adipogenicity of cells from bupivacaine hydrochloride (BPVC)-treated and untreated muscle was compared in vitro. Culturing cells from both BPVC-treated and untreated muscles in adipogenic differentiation medium (ADM) for 10 days resulted in the appearance of mature adipocytes, but their number was 3.5-fold higher in cells from BPVC-treated muscle. Temporal expressions of PPARγ and the presence of lipid droplets during adipogenic differentiation were examined. On day 2 of culture in ADM, only cells from BPVC-treated muscle were positive both for PPARγ and lipid droplets. Pref-1 was expressed in cells from untreated muscle, whereas its expression was absent in cells from BPVC-treated muscle. In ADM, the presence of insulin, which negates an inhibitory effect of Pref-1 on adipogenic differentiation, was required for PPARγ2 expression in cells from untreated muscle, but not for cells from BPVC-treated muscle. These results indicate that BPVC-induced degenerative/regenerative changes in muscle lead to increased adipogenicity of cells, and suggest that this increased adipogenicity not only involves an increase in the number of cells having adipogenic potential, but also contributes to the progression of these cells toward adipogenic differentiation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16750191</pmid><doi>10.1016/j.yexcr.2006.04.014</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4827 |
| ispartof | Experimental cell research, 2006-09, Vol.312 (15), p.2701-2711 |
| issn | 0014-4827 1090-2422 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68791310 |
| source | ScienceDirect Freedom Collection |
| subjects | Adipocytes Adipocytes - cytology Adipocytes - drug effects Adipocytes - metabolism Adipogenesis Adipogenesis - drug effects Adipose tissue development Animals Bupivacaine - metabolism Bupivacaine - pharmacology CCAAT-Enhancer-Binding Protein-alpha - metabolism Cell Differentiation Cells Cells, Cultured Gene expression Immunohistochemistry Insulin - metabolism Male Muscle, Skeletal - drug effects Muscle, Skeletal - physiology Muscular system MyoD Protein - metabolism PPAR gamma - metabolism Progenitor cells Rats Rats, Wistar Regeneration - drug effects Skeletal muscle Tissues |
| title | Increased adipogenicity of cells from regenerating skeletal muscle |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T08%3A43%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20adipogenicity%20of%20cells%20from%20regenerating%20skeletal%20muscle&rft.jtitle=Experimental%20cell%20research&rft.au=Yamanouchi,%20Keitaro&rft.date=2006-09-10&rft.volume=312&rft.issue=15&rft.spage=2701&rft.epage=2711&rft.pages=2701-2711&rft.issn=0014-4827&rft.eissn=1090-2422&rft_id=info:doi/10.1016/j.yexcr.2006.04.014&rft_dat=%3Cproquest_cross%3E1173288801%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c450t-74033eb60031ad2a8681f7d08cdfc58f89d3e1e8a83c5077a65a06c9244942ba3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=194672763&rft_id=info:pmid/16750191&rfr_iscdi=true |