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Inductive effects of dexamethasone on the mineralization and the osteoblastic gene expressions in mature osteoblast-like ROS17/2.8 cells
We examined the effects of dexamethasone (Dex), a synthetic glucocorticoid, on the formation of mineralized bone nodules and the gene expressions of the late osteoblastic markers, bone sialoprotein (BSP), osteocalcin (OC), and osteopontin (OPN) in mature osteoblast ROS17/2.8 cells. Treatment of ROS1...
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| Published in: | Biochemical and biophysical research communications 2007-10, Vol.362 (2), p.368-373 |
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| container_end_page | 373 |
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| container_start_page | 368 |
| container_title | Biochemical and biophysical research communications |
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| creator | Mikami, Yoshikazu Omoteyama, Kazuki Kato, Shigeyuki Takagi, Minoru |
| description | We examined the effects of dexamethasone (Dex), a synthetic glucocorticoid, on the formation of mineralized bone nodules and the gene expressions of the late osteoblastic markers, bone sialoprotein (BSP), osteocalcin (OC), and osteopontin (OPN) in mature osteoblast ROS17/2.8 cells. Treatment of ROS17/2.8 cells with Dex resulted in the induction of mineralization accompanied with increasing BSP and OC expressions. Previous reports have demonstrated that BSP and OC expressions are regulated by Runx2. Then, we hypothesized that Dex might promote osteoblastic differentiation and mineralization on ROS17/2.8 by Runx2. In this study, no effect was observed in mRNA and protein expression of Runx2. However, the transcriptional activity of Runx2 was enhanced by Dex treatment. Furthermore, the Dex-induced BSP and OC expressions decreased after the transfection of Runx2 small-interfering RNAs (siRNAs). These results suggested that the enhancement of Runx2 transcriptional activity by Dex treatment may be followed by the activation of osteoblast marker genes, such as BSP and OC to thereby produce a bone-specific matrix that subsequently becomes mineralized. |
| doi_str_mv | 10.1016/j.bbrc.2007.07.192 |
| format | article |
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Treatment of ROS17/2.8 cells with Dex resulted in the induction of mineralization accompanied with increasing BSP and OC expressions. Previous reports have demonstrated that BSP and OC expressions are regulated by Runx2. Then, we hypothesized that Dex might promote osteoblastic differentiation and mineralization on ROS17/2.8 by Runx2. In this study, no effect was observed in mRNA and protein expression of Runx2. However, the transcriptional activity of Runx2 was enhanced by Dex treatment. Furthermore, the Dex-induced BSP and OC expressions decreased after the transfection of Runx2 small-interfering RNAs (siRNAs). 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Treatment of ROS17/2.8 cells with Dex resulted in the induction of mineralization accompanied with increasing BSP and OC expressions. Previous reports have demonstrated that BSP and OC expressions are regulated by Runx2. Then, we hypothesized that Dex might promote osteoblastic differentiation and mineralization on ROS17/2.8 by Runx2. In this study, no effect was observed in mRNA and protein expression of Runx2. However, the transcriptional activity of Runx2 was enhanced by Dex treatment. Furthermore, the Dex-induced BSP and OC expressions decreased after the transfection of Runx2 small-interfering RNAs (siRNAs). These results suggested that the enhancement of Runx2 transcriptional activity by Dex treatment may be followed by the activation of osteoblast marker genes, such as BSP and OC to thereby produce a bone-specific matrix that subsequently becomes mineralized.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Anthraquinones - chemistry</subject><subject>Blotting, Western</subject><subject>Calcification, Physiologic - drug effects</subject><subject>Calcium - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Line, Tumor</subject><subject>Core Binding Factor Alpha 1 Subunit - genetics</subject><subject>Core Binding Factor Alpha 1 Subunit - metabolism</subject><subject>Dexamethasone</subject><subject>Dexamethasone - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Gene Expression - drug effects</subject><subject>Glucocorticoids - pharmacology</subject><subject>Histocytochemistry</subject><subject>Integrin-Binding Sialoprotein</subject><subject>Mineralization</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - metabolism</subject><subject>Osteocalcin - genetics</subject><subject>Osteocalcin - metabolism</subject><subject>Osteopontin - genetics</subject><subject>Osteopontin - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Small Interfering - genetics</subject><subject>ROS17/2.8</subject><subject>Runx2</subject><subject>Sialoglycoproteins - genetics</subject><subject>Sialoglycoproteins - metabolism</subject><subject>Time Factors</subject><subject>Transfection</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkU1rFTEUhoMo9rb6B1xIVu5mepLcmcyAGylVC4WCH-AuZJITm-vM5JpkSvUX-LPN9F7QlcKBwMtzXsJ5CHnBoGbA2vNdPQzR1BxA1mVYzx-RDYMeKs5g-5hsAKCteM--nJDTlHYAjG3b_ik5YVKClJJvyK-r2S4m-zuk6ByanGhw1OK9njDf6hRmpGGm-Rbp5GeMevQ_dfYl0rN9iEPKGIZRp-wN_YqFx_t9xJQKlKif6aTzEv_mqtF_Q_rh5iOT57zuqMFxTM_IE6fHhM-P7xn5_Pby08X76vrm3dXFm-vKbBuWq170KPggrTV9ywVvxFZ3gqPtus4JDsYxLW3TDyU3DrVzbSO5HICja0A24oy8OvTuY_i-YMpq8mn9gZ4xLEm1HRcgGfwX5MAEAF9BfgBNDClFdGof_aTjD8VAraLUTq2i1CpKlSmiytLLY_syTGj_rBzNFOD1AcByjDuPUSXjcTZofSyalA3-X_2_AX2WphQ</recordid><startdate>20071019</startdate><enddate>20071019</enddate><creator>Mikami, Yoshikazu</creator><creator>Omoteyama, Kazuki</creator><creator>Kato, Shigeyuki</creator><creator>Takagi, Minoru</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20071019</creationdate><title>Inductive effects of dexamethasone on the mineralization and the osteoblastic gene expressions in mature osteoblast-like ROS17/2.8 cells</title><author>Mikami, Yoshikazu ; Omoteyama, Kazuki ; Kato, Shigeyuki ; Takagi, Minoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-939e32b7ddc96232534a832ed888f320cf1a7d59b34acfeaff65727b02ef50753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Anthraquinones - chemistry</topic><topic>Blotting, Western</topic><topic>Calcification, Physiologic - drug effects</topic><topic>Calcium - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Line, Tumor</topic><topic>Core Binding Factor Alpha 1 Subunit - genetics</topic><topic>Core Binding Factor Alpha 1 Subunit - metabolism</topic><topic>Dexamethasone</topic><topic>Dexamethasone - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Gene Expression - drug effects</topic><topic>Glucocorticoids - pharmacology</topic><topic>Histocytochemistry</topic><topic>Integrin-Binding Sialoprotein</topic><topic>Mineralization</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - metabolism</topic><topic>Osteocalcin - genetics</topic><topic>Osteocalcin - metabolism</topic><topic>Osteopontin - genetics</topic><topic>Osteopontin - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Small Interfering - genetics</topic><topic>ROS17/2.8</topic><topic>Runx2</topic><topic>Sialoglycoproteins - genetics</topic><topic>Sialoglycoproteins - metabolism</topic><topic>Time Factors</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mikami, Yoshikazu</creatorcontrib><creatorcontrib>Omoteyama, Kazuki</creatorcontrib><creatorcontrib>Kato, Shigeyuki</creatorcontrib><creatorcontrib>Takagi, Minoru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mikami, Yoshikazu</au><au>Omoteyama, Kazuki</au><au>Kato, Shigeyuki</au><au>Takagi, Minoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inductive effects of dexamethasone on the mineralization and the osteoblastic gene expressions in mature osteoblast-like ROS17/2.8 cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2007-10-19</date><risdate>2007</risdate><volume>362</volume><issue>2</issue><spage>368</spage><epage>373</epage><pages>368-373</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>We examined the effects of dexamethasone (Dex), a synthetic glucocorticoid, on the formation of mineralized bone nodules and the gene expressions of the late osteoblastic markers, bone sialoprotein (BSP), osteocalcin (OC), and osteopontin (OPN) in mature osteoblast ROS17/2.8 cells. Treatment of ROS17/2.8 cells with Dex resulted in the induction of mineralization accompanied with increasing BSP and OC expressions. Previous reports have demonstrated that BSP and OC expressions are regulated by Runx2. Then, we hypothesized that Dex might promote osteoblastic differentiation and mineralization on ROS17/2.8 by Runx2. In this study, no effect was observed in mRNA and protein expression of Runx2. However, the transcriptional activity of Runx2 was enhanced by Dex treatment. Furthermore, the Dex-induced BSP and OC expressions decreased after the transfection of Runx2 small-interfering RNAs (siRNAs). These results suggested that the enhancement of Runx2 transcriptional activity by Dex treatment may be followed by the activation of osteoblast marker genes, such as BSP and OC to thereby produce a bone-specific matrix that subsequently becomes mineralized.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17707772</pmid><doi>10.1016/j.bbrc.2007.07.192</doi><tpages>6</tpages></addata></record> |
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| source | ScienceDirect Journals |
| subjects | Alkaline Phosphatase - metabolism Animals Anthraquinones - chemistry Blotting, Western Calcification, Physiologic - drug effects Calcium - metabolism Cell Differentiation - drug effects Cell Differentiation - genetics Cell Line, Tumor Core Binding Factor Alpha 1 Subunit - genetics Core Binding Factor Alpha 1 Subunit - metabolism Dexamethasone Dexamethasone - pharmacology Dose-Response Relationship, Drug Gene Expression - drug effects Glucocorticoids - pharmacology Histocytochemistry Integrin-Binding Sialoprotein Mineralization Osteoblasts - drug effects Osteoblasts - metabolism Osteocalcin - genetics Osteocalcin - metabolism Osteopontin - genetics Osteopontin - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Small Interfering - genetics ROS17/2.8 Runx2 Sialoglycoproteins - genetics Sialoglycoproteins - metabolism Time Factors Transfection |
| title | Inductive effects of dexamethasone on the mineralization and the osteoblastic gene expressions in mature osteoblast-like ROS17/2.8 cells |
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