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Cytotoxic Proteins Combined with Prodigiosin Obtained from Serratia marcescens Have Both Broad and Selective Cytotoxic Activity on Tumor Cells
Cytotoxic proteins and prodigiosin obtained from Serratia marcescens strains are known to induce tumor cell death, nevertheless its combination has not been studied. In this paper we evaluate the combined effects of these molecules in a panel of tumor cell lines. The results showed a marked inhibito...
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| Published in: | Journal of chemotherapy (Florence) 2006-04, Vol.18 (2), p.172-181 |
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| Main Authors: | , , , , , , , , , , , , , |
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| container_end_page | 181 |
| container_issue | 2 |
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| container_title | Journal of chemotherapy (Florence) |
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| creator | Abrahantes-Pérez, M.C. Reyes-González, J. Véliz Ríos, G. Bequet-Romero, M. Gómez Riera, R. Anais Gasmury, C. Huerta, V. González, L.J. Canino, C. Garcia, J. Váldez, J. Reyes, B. Váldes, R. Martínez, E. |
| description | Cytotoxic proteins and prodigiosin obtained from Serratia marcescens strains are known to induce tumor cell death, nevertheless its combination has not been studied. In this paper we evaluate the combined effects of these molecules in a panel of tumor cell lines. The results showed a marked inhibitory effect on the growth of tumor cell lines derived from tumors (i.e., melanoma) which are highly resistant to conventional anticancer drugs, while normal cells were less sensitive than tumor cells. TUNEL (TdT-mediated dUTP nick end labeling) and electrophoresis of HEp-2 cell DNA treated with MG2327 preparation [containing the P50 protein belonging to the serralysins and prodigiosin, from S. marcescens CMIB4202] showed a pattern of DNA fragments typically associated with apoptosis. Interestingly, prodigiosin enhanced by 1.6-fold the cytotoxic effect of P50 when acting in combination on HEp-2 cells. The broad cytotoxic activity of the combination on tumor cells as well as its selectivity open new frontiers in cancer therapy. |
| doi_str_mv | 10.1179/joc.2006.18.2.172 |
| format | article |
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In this paper we evaluate the combined effects of these molecules in a panel of tumor cell lines. The results showed a marked inhibitory effect on the growth of tumor cell lines derived from tumors (i.e., melanoma) which are highly resistant to conventional anticancer drugs, while normal cells were less sensitive than tumor cells. TUNEL (TdT-mediated dUTP nick end labeling) and electrophoresis of HEp-2 cell DNA treated with MG2327 preparation [containing the P50 protein belonging to the serralysins and prodigiosin, from S. marcescens CMIB4202] showed a pattern of DNA fragments typically associated with apoptosis. Interestingly, prodigiosin enhanced by 1.6-fold the cytotoxic effect of P50 when acting in combination on HEp-2 cells. The broad cytotoxic activity of the combination on tumor cells as well as its selectivity open new frontiers in cancer therapy.</description><identifier>ISSN: 1120-009X</identifier><identifier>EISSN: 1973-9478</identifier><identifier>DOI: 10.1179/joc.2006.18.2.172</identifier><identifier>PMID: 16736886</identifier><language>eng</language><publisher>Firenze: Taylor & Francis</publisher><subject>Anti-Bacterial Agents - isolation & purification ; Anti-Bacterial Agents - pharmacology ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; anticancer ; Antineoplastic Agents - pharmacology ; antitumor ; Apoptosis ; Bacterial Proteins - isolation & purification ; Bacterial Proteins - pharmacology ; Biological and medical sciences ; cancer therapy ; CMIB4202 ; Cytotoxic protein ; DNA, Neoplasm - analysis ; Drug Therapy, Combination ; Electrophoresis, Agar Gel ; Humans ; In Situ Nick-End Labeling ; Medical sciences ; MG2327 ; Neoplasms - drug therapy ; Pharmacology. 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The broad cytotoxic activity of the combination on tumor cells as well as its selectivity open new frontiers in cancer therapy.</description><subject>Anti-Bacterial Agents - isolation & purification</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>anticancer</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>antitumor</subject><subject>Apoptosis</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Biological and medical sciences</subject><subject>cancer therapy</subject><subject>CMIB4202</subject><subject>Cytotoxic protein</subject><subject>DNA, Neoplasm - analysis</subject><subject>Drug Therapy, Combination</subject><subject>Electrophoresis, Agar Gel</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Medical sciences</subject><subject>MG2327</subject><subject>Neoplasms - drug therapy</subject><subject>Pharmacology. Drug treatments</subject><subject>prodigiosin</subject><subject>Prodigiosin - isolation & purification</subject><subject>Prodigiosin - pharmacology</subject><subject>serralisin</subject><subject>Serratia marcescens</subject><subject>Serratia marcescens - chemistry</subject><subject>Tumor Cells, Cultured</subject><issn>1120-009X</issn><issn>1973-9478</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp1kc9uFSEUxonR2KbtA7gxbHQ3IzCTARI37UStSZM2sU26I2f4ozTMUIHbel_CZ5bxXnNXsoGc8_s-OHwIvaGkpZTLDw9Rt4yQoaWiZS3l7AU6ppJ3jey5eFnPlJGGEHl_hM5yfiB1DYx3cniNjujAu0GI4Rj9HrcllvjLa3yTYrF-yXiM8-QXa_CzLz_WsvHffcx-wddTgb8dl-KMv9mUoHjAMyRts7ZVewlPFl_EqrtIEQyGxVQuWF18bRwuO18LvmxxXPDtZo4JjzaEfIpeOQjZnu33E3T3-dPteNlcXX_5Op5fNboTrDST6y0hrLdWMKah64lgVpIetJFEczdAL3vpmGXCcTlNhknZEzCC0UlSTrsT9H7n-5jiz43NRc2-DhACLDZushoEYXwH0h2oU8w5Wacek6_zbhUlas1B1RzUmoOiQjFVc6iat3vzzTRbc1Dsf70C7_YAZA3BJVi0zweOCy46thp93HF-cTHN8BxTMKrANsT0T9T9_x1_AKW5p2w</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Abrahantes-Pérez, M.C.</creator><creator>Reyes-González, J.</creator><creator>Véliz Ríos, G.</creator><creator>Bequet-Romero, M.</creator><creator>Gómez Riera, R.</creator><creator>Anais Gasmury, C.</creator><creator>Huerta, V.</creator><creator>González, L.J.</creator><creator>Canino, C.</creator><creator>Garcia, J.</creator><creator>Váldez, J.</creator><creator>Reyes, B.</creator><creator>Váldes, R.</creator><creator>Martínez, E.</creator><general>Taylor & Francis</general><general>EIFT</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Cytotoxic Proteins Combined with Prodigiosin Obtained from Serratia marcescens Have Both Broad and Selective Cytotoxic Activity on Tumor Cells</title><author>Abrahantes-Pérez, M.C. ; Reyes-González, J. ; Véliz Ríos, G. ; Bequet-Romero, M. ; Gómez Riera, R. ; Anais Gasmury, C. ; Huerta, V. ; González, L.J. ; Canino, C. ; Garcia, J. ; Váldez, J. ; Reyes, B. ; Váldes, R. ; Martínez, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-bf4e0024ee822ca34082e904acd90c7f6a4949f2e28f79bbd29940ad821b91713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics. 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In this paper we evaluate the combined effects of these molecules in a panel of tumor cell lines. The results showed a marked inhibitory effect on the growth of tumor cell lines derived from tumors (i.e., melanoma) which are highly resistant to conventional anticancer drugs, while normal cells were less sensitive than tumor cells. TUNEL (TdT-mediated dUTP nick end labeling) and electrophoresis of HEp-2 cell DNA treated with MG2327 preparation [containing the P50 protein belonging to the serralysins and prodigiosin, from S. marcescens CMIB4202] showed a pattern of DNA fragments typically associated with apoptosis. Interestingly, prodigiosin enhanced by 1.6-fold the cytotoxic effect of P50 when acting in combination on HEp-2 cells. The broad cytotoxic activity of the combination on tumor cells as well as its selectivity open new frontiers in cancer therapy.</abstract><cop>Firenze</cop><pub>Taylor & Francis</pub><pmid>16736886</pmid><doi>10.1179/joc.2006.18.2.172</doi><tpages>10</tpages></addata></record> |
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| source | Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Medical Collection (Reading list) |
| subjects | Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Antibiotics. Antiinfectious agents. Antiparasitic agents anticancer Antineoplastic Agents - pharmacology antitumor Apoptosis Bacterial Proteins - isolation & purification Bacterial Proteins - pharmacology Biological and medical sciences cancer therapy CMIB4202 Cytotoxic protein DNA, Neoplasm - analysis Drug Therapy, Combination Electrophoresis, Agar Gel Humans In Situ Nick-End Labeling Medical sciences MG2327 Neoplasms - drug therapy Pharmacology. Drug treatments prodigiosin Prodigiosin - isolation & purification Prodigiosin - pharmacology serralisin Serratia marcescens Serratia marcescens - chemistry Tumor Cells, Cultured |
| title | Cytotoxic Proteins Combined with Prodigiosin Obtained from Serratia marcescens Have Both Broad and Selective Cytotoxic Activity on Tumor Cells |
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