Simultaneous retention index analysis of urinary amino acids and carboxylic acids for graphic recognition of abnormal state

Simultaneous profiling analysis of urinary amino acids (AAs) and carboxylic acids (CAs) was combined with retention index (I) analysis for graphic recognition of abnormal metabolic state. The temperature-programmed I values of the AA and CA standards measured as ethoxycarbonyl (EOC)/methoxime (MO)/t...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-07, Vol.821 (1), p.94-104
Main Authors: PAIK, Man-Jeong, LEE, Hong-Jin, KIM, Kyoung-Rae
Format: Article
Language:English
Subjects:
Acetamides
Amino Acids - urine
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carboxylic Acids - urine
Child
Child, Preschool
Chromatography, Gas - methods
Fluoroacetates
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Hydroxylamines - chemistry
Infant
Maple Syrup Urine Disease - urine
Medical sciences
Metabolism, Inborn Errors - urine
Methylmalonic Acid - urine
Organosilicon Compounds - chemistry
Pentanoic Acids - urine
Pharmacology. Drug treatments
Phenylketonurias - urine
Trifluoroacetic Acid - chemistry
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Summary:Simultaneous profiling analysis of urinary amino acids (AAs) and carboxylic acids (CAs) was combined with retention index (I) analysis for graphic recognition of abnormal metabolic state. The temperature-programmed I values of the AA and CA standards measured as ethoxycarbonyl (EOC)/methoxime (MO)/tert-butyldimethylsilyl (TBDMS) derivatives were used as the reference I values. Urine samples were subjected to the sequential EOC, MO and TBDMS reactions for the analysis by gas chromatography (GC) and GC-mass spectrometry. The complex GC profiles were then transformed into their respective I patterns in bar graphic forms by plotting the normalized peak area ratios (%) of the identified AAs and CAs against their reference I values as the identification numbers. When the present method was applied to infant urine specimens from normal controls and patients with inherited metabolic diseases such as phenylketonuria, maple syrup urine disease, methylmalonic aciduria or isovaleric aciduria, each I pattern of bar graph more distinctly displayed quantitative abundances of urinary AAs and CAs in qualitative I scale, thus allowing graphic discrimination between normal and abnormal states.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2005.04.011