Effect of reduced oocyte aging on the outcome of rescue intracytoplasmic sperm injection

To evaluate the results of a novel protocol that allows to rescue IVF unfertilized oocytes by intracytoplasmic sperm injection (ICSI). Prospective clinical trial. Private reproductive medical center. Thirty patients undergoing IVF. Controlled ovarian stimulation (COS), conventional IVF, rescue ICSI,...

Full description

Saved in:
Bibliographic Details
Published in:Fertility and sterility 2006-04, Vol.85 (4), p.901-906
Main Authors: Nagy, Zsolt P., Rienzi, Laura F., Ubaldi, Filippo M., Greco, Ermanno, Massey, Joe B., Kort, Hilton I.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Cellular Senescence - physiology
Female
fertilization
Gynecology. Andrology. Obstetrics
Humans
ICSI
IVF
Male
Medical sciences
microinjection
Oocytes - physiology
Pregnancy
Pregnancy Rate
Prospective Studies
reinsemination
Sperm Count
Sperm Injections, Intracytoplasmic - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To evaluate the results of a novel protocol that allows to rescue IVF unfertilized oocytes by intracytoplasmic sperm injection (ICSI). Prospective clinical trial. Private reproductive medical center. Thirty patients undergoing IVF. Controlled ovarian stimulation (COS), conventional IVF, rescue ICSI, embryo culture, and embryo transfer. Identification of unfertilized IVF oocytes 6 hours after insemination and fertilization, and developmental rates of those oocytes after rescue microinjection, as well implantation and pregnancy rates (PR). All oocytes (392) from 30 patients were inseminated with standard IVF 3 hours after ovum pick-up. Polar body (PB) status was checked at decumulation and rechecked 3 hours later. Eighty-two oocytes were fertilized after IVF alone and 184 nonactivated oocytes (failed fertilization) were rescue microinjected and 166 of them fertilized (20 patients). Cleavage stage on day 2 was significantly more advanced and embryo grade was higher after standard IVF fertilization than after rescue ICSI. Eight of the 30 embryos transferred were implanted in the IVF-only patients (27%) and 8 of 68 embryos in the rescue ICSI patients (12%). Rescue ICSI of unfertilized IVF oocytes 6 hours after insemination (9 hours after egg retrieval) can provide normal fertilization, embryo development, and pregnancy; however, corresponding outcome parameters tend to be impaired in comparison to the standard IVF fertilization results.
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2005.09.029