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Survey of bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil
Objective The ocular microflora in dogs has not been established in north-east Brazil. Thus, the main aim of this research was to determine the bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil. Animals studied T...
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| Published in: | Veterinary ophthalmology 2005, Vol.8 (1), p.33-37 |
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| container_end_page | 37 |
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| container_title | Veterinary ophthalmology |
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| creator | Prado, M.R Rocha, M.F.G Brito, E.H.S Girao, M.D Monteiro, A.J Teixeira, M.F.S Sidrim, J.J.C |
| description | Objective The ocular microflora in dogs has not been established in north-east Brazil. Thus, the main aim of this research was to determine the bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil. Animals studied This study included 60 healthy dogs, 15 dogs with unilateral corneal ulcer, and three dogs with bilateral corneal ulcers. Procedure Samples were taken by a calibrated platinum loop (1 microliter) placed directly onto the conjunctival sac and on sterile blood agar. The clinical specimens were incubated at 37 degrees C in an atmosphere of 5% CO2 for 48 h. Results Of the 120 samples from healthy dogs, only 47 (39%) had positive culture for bacteria, while all of the specimens from eyes with corneal ulcer were positive for bacterial growth. The group of dogs with corneal ulcer had a higher (P < 0.05) number of colony-forming units (CFU) per plate than the group of healthy animals. Of the 59 isolates from healthy eyes, only nine (15.3%) had more than 50 CFU per plate, while in the group of dogs with corneal ulcer, 23 (62.2%) of the 37 isolates presented more than 50 CFU per plate. In both groups Gram-positive bacteria (86.5%) predominated over Gram-negative (13.5%). Staphylococcus spp. was the most frequently isolated genus and S. intermedius predominated in both groups. Conclusion The results of our study are directly applicable to initiate rational, preventive and therapeutic measures with greater accuracy in dogs with corneal ulcer. |
| doi_str_mv | 10.1111/j.1463-5224.2005.04061.x |
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Thus, the main aim of this research was to determine the bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil. Animals studied This study included 60 healthy dogs, 15 dogs with unilateral corneal ulcer, and three dogs with bilateral corneal ulcers. Procedure Samples were taken by a calibrated platinum loop (1 microliter) placed directly onto the conjunctival sac and on sterile blood agar. The clinical specimens were incubated at 37 degrees C in an atmosphere of 5% CO2 for 48 h. Results Of the 120 samples from healthy dogs, only 47 (39%) had positive culture for bacteria, while all of the specimens from eyes with corneal ulcer were positive for bacterial growth. The group of dogs with corneal ulcer had a higher (P < 0.05) number of colony-forming units (CFU) per plate than the group of healthy animals. Of the 59 isolates from healthy eyes, only nine (15.3%) had more than 50 CFU per plate, while in the group of dogs with corneal ulcer, 23 (62.2%) of the 37 isolates presented more than 50 CFU per plate. In both groups Gram-positive bacteria (86.5%) predominated over Gram-negative (13.5%). Staphylococcus spp. was the most frequently isolated genus and S. intermedius predominated in both groups. Conclusion The results of our study are directly applicable to initiate rational, preventive and therapeutic measures with greater accuracy in dogs with corneal ulcer.</description><identifier>ISSN: 1463-5216</identifier><identifier>EISSN: 1463-5224</identifier><identifier>DOI: 10.1111/j.1463-5224.2005.04061.x</identifier><identifier>PMID: 15644098</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; bacteria ; bacterial infections ; Brazil - epidemiology ; Case-Control Studies ; Conjunctiva - microbiology ; conjunctival sac ; corneal ulcer ; Corneal Ulcer - epidemiology ; Corneal Ulcer - veterinary ; diagnostic techniques ; disease diagnosis ; dog ; dog diseases ; Dog Diseases - epidemiology ; Dog Diseases - microbiology ; dogs ; Dogs - microbiology ; Female ; Gram-negative bacteria ; Gram-Negative Bacteria - isolation & purification ; Gram-positive bacteria ; Gram-Positive Bacteria - isolation & purification ; keratitis ; lacrimal apparatus ; Male ; Staphylococcus ; Staphylococcus intermedius ; Stem Cells ; validity</subject><ispartof>Veterinary ophthalmology, 2005, Vol.8 (1), p.33-37</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4611-66809dd891dfe16b648798880ec0b97a1789480d92b66255104499a295bcad83</citedby><cites>FETCH-LOGICAL-c4611-66809dd891dfe16b648798880ec0b97a1789480d92b66255104499a295bcad83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1463-5224.2005.04061.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1463-5224.2005.04061.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,4043,27956,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15644098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prado, M.R</creatorcontrib><creatorcontrib>Rocha, M.F.G</creatorcontrib><creatorcontrib>Brito, E.H.S</creatorcontrib><creatorcontrib>Girao, M.D</creatorcontrib><creatorcontrib>Monteiro, A.J</creatorcontrib><creatorcontrib>Teixeira, M.F.S</creatorcontrib><creatorcontrib>Sidrim, J.J.C</creatorcontrib><title>Survey of bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil</title><title>Veterinary ophthalmology</title><addtitle>Vet Ophthalmol</addtitle><description>Objective The ocular microflora in dogs has not been established in north-east Brazil. Thus, the main aim of this research was to determine the bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil. Animals studied This study included 60 healthy dogs, 15 dogs with unilateral corneal ulcer, and three dogs with bilateral corneal ulcers. Procedure Samples were taken by a calibrated platinum loop (1 microliter) placed directly onto the conjunctival sac and on sterile blood agar. The clinical specimens were incubated at 37 degrees C in an atmosphere of 5% CO2 for 48 h. Results Of the 120 samples from healthy dogs, only 47 (39%) had positive culture for bacteria, while all of the specimens from eyes with corneal ulcer were positive for bacterial growth. The group of dogs with corneal ulcer had a higher (P < 0.05) number of colony-forming units (CFU) per plate than the group of healthy animals. Of the 59 isolates from healthy eyes, only nine (15.3%) had more than 50 CFU per plate, while in the group of dogs with corneal ulcer, 23 (62.2%) of the 37 isolates presented more than 50 CFU per plate. In both groups Gram-positive bacteria (86.5%) predominated over Gram-negative (13.5%). Staphylococcus spp. was the most frequently isolated genus and S. intermedius predominated in both groups. Conclusion The results of our study are directly applicable to initiate rational, preventive and therapeutic measures with greater accuracy in dogs with corneal ulcer.</description><subject>Animals</subject><subject>bacteria</subject><subject>bacterial infections</subject><subject>Brazil - epidemiology</subject><subject>Case-Control Studies</subject><subject>Conjunctiva - microbiology</subject><subject>conjunctival sac</subject><subject>corneal ulcer</subject><subject>Corneal Ulcer - epidemiology</subject><subject>Corneal Ulcer - veterinary</subject><subject>diagnostic techniques</subject><subject>disease diagnosis</subject><subject>dog</subject><subject>dog diseases</subject><subject>Dog Diseases - epidemiology</subject><subject>Dog Diseases - microbiology</subject><subject>dogs</subject><subject>Dogs - microbiology</subject><subject>Female</subject><subject>Gram-negative bacteria</subject><subject>Gram-Negative Bacteria - isolation & purification</subject><subject>Gram-positive bacteria</subject><subject>Gram-Positive Bacteria - isolation & purification</subject><subject>keratitis</subject><subject>lacrimal apparatus</subject><subject>Male</subject><subject>Staphylococcus</subject><subject>Staphylococcus intermedius</subject><subject>Stem Cells</subject><subject>validity</subject><issn>1463-5216</issn><issn>1463-5224</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkd9u0zAYxSMEYmPwCuArrkiwE8exb5BYYYVpMKQVuLS-OG7nzomHnWztXoMXxmmqcgm-8ZG-3zn-c5IEEZyRuN6uM0JZkZZ5TrMc4zLDFDOSbR4lx4fB44Mm7Ch5FsIaY1yUuHqaHJGSUYoFP05-Xw3-Tm-RW6IaVK-9AYtao7xzfgWdCW1ApkP9tUbKdeuhU725i0gANXqUNZ1RYO0Wdc63cdC4VUDQNZO4N_01GqzSHqJPo5ud6M0u9Mz5Hqx-gDdopsHH7dTDg7HPkydLsEG_2O8nyeLs42L2Kb24nH-evb9IFWWEpIxxLJqGC9IsNWE1o7wSnHOsFa5FBaTignLciLxmLC9LgikVAnJR1goaXpwkr6fYW-9-DTr0sjVBaWuh024IklUFKwuS_xMkQuSkECSCfALj94Xg9VLeetOC30qC5VicXMuxEzn2I8fi5K44uYnWl_szhrrVzV_jvqkIvJuAe2P19r-D5Y_LbzsZA9IpwIRebw4B4G_Gl1al_Pl1Lqsvs_n5-YeFPI38q4lfgpOw8ibI71c5JgWO1-GY8OIP95HAeQ</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>Prado, M.R</creator><creator>Rocha, M.F.G</creator><creator>Brito, E.H.S</creator><creator>Girao, M.D</creator><creator>Monteiro, A.J</creator><creator>Teixeira, M.F.S</creator><creator>Sidrim, J.J.C</creator><general>Blackwell Science Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2005</creationdate><title>Survey of bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil</title><author>Prado, M.R ; Rocha, M.F.G ; Brito, E.H.S ; Girao, M.D ; Monteiro, A.J ; Teixeira, M.F.S ; Sidrim, J.J.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4611-66809dd891dfe16b648798880ec0b97a1789480d92b66255104499a295bcad83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>bacteria</topic><topic>bacterial infections</topic><topic>Brazil - epidemiology</topic><topic>Case-Control Studies</topic><topic>Conjunctiva - microbiology</topic><topic>conjunctival sac</topic><topic>corneal ulcer</topic><topic>Corneal Ulcer - epidemiology</topic><topic>Corneal Ulcer - veterinary</topic><topic>diagnostic techniques</topic><topic>disease diagnosis</topic><topic>dog</topic><topic>dog diseases</topic><topic>Dog Diseases - epidemiology</topic><topic>Dog Diseases - microbiology</topic><topic>dogs</topic><topic>Dogs - microbiology</topic><topic>Female</topic><topic>Gram-negative bacteria</topic><topic>Gram-Negative Bacteria - isolation & purification</topic><topic>Gram-positive bacteria</topic><topic>Gram-Positive Bacteria - isolation & purification</topic><topic>keratitis</topic><topic>lacrimal apparatus</topic><topic>Male</topic><topic>Staphylococcus</topic><topic>Staphylococcus intermedius</topic><topic>Stem Cells</topic><topic>validity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prado, M.R</creatorcontrib><creatorcontrib>Rocha, M.F.G</creatorcontrib><creatorcontrib>Brito, E.H.S</creatorcontrib><creatorcontrib>Girao, M.D</creatorcontrib><creatorcontrib>Monteiro, A.J</creatorcontrib><creatorcontrib>Teixeira, M.F.S</creatorcontrib><creatorcontrib>Sidrim, J.J.C</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prado, M.R</au><au>Rocha, M.F.G</au><au>Brito, E.H.S</au><au>Girao, M.D</au><au>Monteiro, A.J</au><au>Teixeira, M.F.S</au><au>Sidrim, J.J.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Survey of bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil</atitle><jtitle>Veterinary ophthalmology</jtitle><addtitle>Vet Ophthalmol</addtitle><date>2005</date><risdate>2005</risdate><volume>8</volume><issue>1</issue><spage>33</spage><epage>37</epage><pages>33-37</pages><issn>1463-5216</issn><eissn>1463-5224</eissn><notes>http://www.blackwell-science.com/products/journals/jnltitle.htm</notes><notes>ark:/67375/WNG-7MCGJJDT-B</notes><notes>ArticleID:VOP04061</notes><notes>istex:5FC0BD51FC568B15CE7DF007163902F8511870E3</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Objective The ocular microflora in dogs has not been established in north-east Brazil. Thus, the main aim of this research was to determine the bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil. Animals studied This study included 60 healthy dogs, 15 dogs with unilateral corneal ulcer, and three dogs with bilateral corneal ulcers. Procedure Samples were taken by a calibrated platinum loop (1 microliter) placed directly onto the conjunctival sac and on sterile blood agar. The clinical specimens were incubated at 37 degrees C in an atmosphere of 5% CO2 for 48 h. Results Of the 120 samples from healthy dogs, only 47 (39%) had positive culture for bacteria, while all of the specimens from eyes with corneal ulcer were positive for bacterial growth. The group of dogs with corneal ulcer had a higher (P < 0.05) number of colony-forming units (CFU) per plate than the group of healthy animals. Of the 59 isolates from healthy eyes, only nine (15.3%) had more than 50 CFU per plate, while in the group of dogs with corneal ulcer, 23 (62.2%) of the 37 isolates presented more than 50 CFU per plate. In both groups Gram-positive bacteria (86.5%) predominated over Gram-negative (13.5%). Staphylococcus spp. was the most frequently isolated genus and S. intermedius predominated in both groups. Conclusion The results of our study are directly applicable to initiate rational, preventive and therapeutic measures with greater accuracy in dogs with corneal ulcer.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15644098</pmid><doi>10.1111/j.1463-5224.2005.04061.x</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals bacteria bacterial infections Brazil - epidemiology Case-Control Studies Conjunctiva - microbiology conjunctival sac corneal ulcer Corneal Ulcer - epidemiology Corneal Ulcer - veterinary diagnostic techniques disease diagnosis dog dog diseases Dog Diseases - epidemiology Dog Diseases - microbiology dogs Dogs - microbiology Female Gram-negative bacteria Gram-Negative Bacteria - isolation & purification Gram-positive bacteria Gram-Positive Bacteria - isolation & purification keratitis lacrimal apparatus Male Staphylococcus Staphylococcus intermedius Stem Cells validity |
| title | Survey of bacterial microorganisms in the conjunctival sac of clinically normal dogs and dogs with ulcerative keratitis in Fortaleza, Ceara, Brazil |
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