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Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007–2008 respiratory season
Abstract Background The H275Y mutation (H274Y in N2 numbering) in the neuraminidase (NA) gene (segment 6) of the influenza virus A (H1N1) genome is linked to oseltamivir resistance. Objectives To determine the percentage of influenza virus A (H1N1) isolates that carry the H275Y mutation in the NA ge...
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Published in: | Journal of clinical virology 2009-06, Vol.45 (2), p.142-145 |
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description | Abstract Background The H275Y mutation (H274Y in N2 numbering) in the neuraminidase (NA) gene (segment 6) of the influenza virus A (H1N1) genome is linked to oseltamivir resistance. Objectives To determine the percentage of influenza virus A (H1N1) isolates that carry the H275Y mutation in the NA gene in Toronto, Ontario, Canada and to characterize select oseltamivir resistant and susceptible isolates using sequence analysis. Study design Sanger sequencing was used to determine strain type and H275Y mutations based on partial sequencing of the hemagglutinin (HA) (segment 4) and NA genes. Mutations in the NS1 gene (segment 8) were determined by Sanger sequencing and pyrosequencing. Statistical analysis of demographics and proportions of H275 and H275Y isolates with mutations was carried out using χ2 analyses. Results The HA gene of influenza virus A (H1N1) isolates collected during the 2007–2008 respiratory season was most like influenza A/Brisbane/59/2007, Clade 2, subclade B. Seventeen percent of these isolates possessed the H275Y NA mutation associated with oseltamivir resistance. H275Y isolates were more likely than H275 isolates to have the mutations A209T and R224G in NS1 ( χ2 = 284.9, df = 2, p < 0.0001). Conclusions During the 2007–2008 influenza season in Toronto, Ontario, Canada, 17% of influenza virus A (H1N1) isolates carried the H275Y mutation associated with oseltamivir resistance. These H275Y isolates were more likely than H275 isolates to exhibit unique microheterogeneity in the gene encoding the NS1 protein. |
doi_str_mv | 10.1016/j.jcv.2009.04.010 |
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Objectives To determine the percentage of influenza virus A (H1N1) isolates that carry the H275Y mutation in the NA gene in Toronto, Ontario, Canada and to characterize select oseltamivir resistant and susceptible isolates using sequence analysis. Study design Sanger sequencing was used to determine strain type and H275Y mutations based on partial sequencing of the hemagglutinin (HA) (segment 4) and NA genes. Mutations in the NS1 gene (segment 8) were determined by Sanger sequencing and pyrosequencing. Statistical analysis of demographics and proportions of H275 and H275Y isolates with mutations was carried out using χ2 analyses. Results The HA gene of influenza virus A (H1N1) isolates collected during the 2007–2008 respiratory season was most like influenza A/Brisbane/59/2007, Clade 2, subclade B. Seventeen percent of these isolates possessed the H275Y NA mutation associated with oseltamivir resistance. H275Y isolates were more likely than H275 isolates to have the mutations A209T and R224G in NS1 ( χ2 = 284.9, df = 2, p < 0.0001). Conclusions During the 2007–2008 influenza season in Toronto, Ontario, Canada, 17% of influenza virus A (H1N1) isolates carried the H275Y mutation associated with oseltamivir resistance. These H275Y isolates were more likely than H275 isolates to exhibit unique microheterogeneity in the gene encoding the NS1 protein.</description><identifier>ISSN: 1386-6532</identifier><identifier>EISSN: 1873-5967</identifier><identifier>DOI: 10.1016/j.jcv.2009.04.010</identifier><identifier>PMID: 19451021</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Allergy and Immunology ; Amino Acid Substitution - genetics ; Antiviral Agents - pharmacology ; Biological and medical sciences ; Child ; Child, Preschool ; Drug Resistance, Viral ; Female ; Fundamental and applied biological sciences. Psychology ; Genetics ; H275Y ; Human viral diseases ; Humans ; Infant ; Infectious Disease ; Infectious diseases ; Influenza ; Influenza A virus ; Influenza A Virus, H1N1 Subtype - genetics ; Influenza A Virus, H1N1 Subtype - isolation & purification ; Influenza, Human - epidemiology ; Influenza, Human - virology ; Male ; Medical sciences ; Microbiology ; Middle Aged ; Miscellaneous ; Molecular Sequence Data ; Mutation, Missense ; Neuraminidase - genetics ; Ontario - epidemiology ; Oseltamivir ; Oseltamivir - pharmacology ; Polymorphism, Genetic ; Pyrosequencing ; Resistance ; Sequence Analysis, DNA ; Viral diseases ; Viral Proteins - genetics ; Virology ; Young Adult</subject><ispartof>Journal of clinical virology, 2009-06, Vol.45 (2), p.142-145</ispartof><rights>2009</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-e338716df964cb067256c526974633874220869728212529cc62e54800d5e99d3</citedby><cites>FETCH-LOGICAL-c533t-e338716df964cb067256c526974633874220869728212529cc62e54800d5e99d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21634509$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19451021$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eshaghi, A</creatorcontrib><creatorcontrib>Bolotin, S</creatorcontrib><creatorcontrib>Burton, L</creatorcontrib><creatorcontrib>Low, D.E</creatorcontrib><creatorcontrib>Mazzulli, T</creatorcontrib><creatorcontrib>Drews, S.J</creatorcontrib><title>Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007–2008 respiratory season</title><title>Journal of clinical virology</title><addtitle>J Clin Virol</addtitle><description>Abstract Background The H275Y mutation (H274Y in N2 numbering) in the neuraminidase (NA) gene (segment 6) of the influenza virus A (H1N1) genome is linked to oseltamivir resistance. Objectives To determine the percentage of influenza virus A (H1N1) isolates that carry the H275Y mutation in the NA gene in Toronto, Ontario, Canada and to characterize select oseltamivir resistant and susceptible isolates using sequence analysis. Study design Sanger sequencing was used to determine strain type and H275Y mutations based on partial sequencing of the hemagglutinin (HA) (segment 4) and NA genes. Mutations in the NS1 gene (segment 8) were determined by Sanger sequencing and pyrosequencing. Statistical analysis of demographics and proportions of H275 and H275Y isolates with mutations was carried out using χ2 analyses. Results The HA gene of influenza virus A (H1N1) isolates collected during the 2007–2008 respiratory season was most like influenza A/Brisbane/59/2007, Clade 2, subclade B. Seventeen percent of these isolates possessed the H275Y NA mutation associated with oseltamivir resistance. H275Y isolates were more likely than H275 isolates to have the mutations A209T and R224G in NS1 ( χ2 = 284.9, df = 2, p < 0.0001). Conclusions During the 2007–2008 influenza season in Toronto, Ontario, Canada, 17% of influenza virus A (H1N1) isolates carried the H275Y mutation associated with oseltamivir resistance. These H275Y isolates were more likely than H275 isolates to exhibit unique microheterogeneity in the gene encoding the NS1 protein.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Allergy and Immunology</subject><subject>Amino Acid Substitution - genetics</subject><subject>Antiviral Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Drug Resistance, Viral</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>H275Y</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infant</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Influenza</subject><subject>Influenza A virus</subject><subject>Influenza A Virus, H1N1 Subtype - genetics</subject><subject>Influenza A Virus, H1N1 Subtype - isolation & purification</subject><subject>Influenza, Human - epidemiology</subject><subject>Influenza, Human - virology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Mutation, Missense</subject><subject>Neuraminidase - genetics</subject><subject>Ontario - epidemiology</subject><subject>Oseltamivir</subject><subject>Oseltamivir - pharmacology</subject><subject>Polymorphism, Genetic</subject><subject>Pyrosequencing</subject><subject>Resistance</subject><subject>Sequence Analysis, DNA</subject><subject>Viral diseases</subject><subject>Viral Proteins - genetics</subject><subject>Virology</subject><subject>Young Adult</subject><issn>1386-6532</issn><issn>1873-5967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFks2KFDEQxxtR3HX1AbxILoqCM1aSTtJBEJZBd4TFPbiCnkI2XT2bsTsZk-6F8eTVs2_ok5hhBgUPeqpK6lcf1L-q6iGFOQUqX6zna3czZwB6DvUcKNyqjmmj-ExoqW4XnzdyJgVnR9W9nNcAVPBa3a2OqK4FBUaPq-9nGHD0jgzepXiNI6a4Kl9-3JLYERwwrXxYkSVT4hPxoesnDF8tufFpyuSUPF3Sd_RZCZDLmGIY43NyEUabfHEWNtjWki7FgYzXSMqg6ue3H8U0JGHe-GTHmLYko80x3K_udLbP-OBgT6oPb15fLpaz84uzt4vT85kTnI8z5LxRVLadlrW7AqmYkE4wqVUtd6GaMWjKizWMMsG0c5KhqBuAVqDWLT-pnuzrblL8MmEezeCzw763AeOUjVScUVnDf8HSR2khaAHpHiwbzDlhZzbJDzZtDQWzE8qsTRHK7IQyUJsiVMl5dCg-XQ3Y_sk4KFOAxwfAZmf7LtngfP7NlRF5LUAX7uWew7KzG4_JZOcxOGx9QjeaNvp_jvHqr2zX--BLw8-4xbyOUwpFDENNZgbM-91F7Q4KdDkmBR_5Lwn8wlo</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Eshaghi, A</creator><creator>Bolotin, S</creator><creator>Burton, L</creator><creator>Low, D.E</creator><creator>Mazzulli, T</creator><creator>Drews, S.J</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007–2008 respiratory season</title><author>Eshaghi, A ; Bolotin, S ; Burton, L ; Low, D.E ; Mazzulli, T ; Drews, S.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-e338716df964cb067256c526974633874220869728212529cc62e54800d5e99d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Allergy and Immunology</topic><topic>Amino Acid Substitution - genetics</topic><topic>Antiviral Agents - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Drug Resistance, Viral</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>H275Y</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infant</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Influenza</topic><topic>Influenza A virus</topic><topic>Influenza A Virus, H1N1 Subtype - genetics</topic><topic>Influenza A Virus, H1N1 Subtype - isolation & purification</topic><topic>Influenza, Human - epidemiology</topic><topic>Influenza, Human - virology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Mutation, Missense</topic><topic>Neuraminidase - genetics</topic><topic>Ontario - epidemiology</topic><topic>Oseltamivir</topic><topic>Oseltamivir - pharmacology</topic><topic>Polymorphism, Genetic</topic><topic>Pyrosequencing</topic><topic>Resistance</topic><topic>Sequence Analysis, DNA</topic><topic>Viral diseases</topic><topic>Viral Proteins - genetics</topic><topic>Virology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eshaghi, A</creatorcontrib><creatorcontrib>Bolotin, S</creatorcontrib><creatorcontrib>Burton, L</creatorcontrib><creatorcontrib>Low, D.E</creatorcontrib><creatorcontrib>Mazzulli, T</creatorcontrib><creatorcontrib>Drews, S.J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eshaghi, A</au><au>Bolotin, S</au><au>Burton, L</au><au>Low, D.E</au><au>Mazzulli, T</au><au>Drews, S.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007–2008 respiratory season</atitle><jtitle>Journal of clinical virology</jtitle><addtitle>J Clin Virol</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>45</volume><issue>2</issue><spage>142</spage><epage>145</epage><pages>142-145</pages><issn>1386-6532</issn><eissn>1873-5967</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Abstract Background The H275Y mutation (H274Y in N2 numbering) in the neuraminidase (NA) gene (segment 6) of the influenza virus A (H1N1) genome is linked to oseltamivir resistance. Objectives To determine the percentage of influenza virus A (H1N1) isolates that carry the H275Y mutation in the NA gene in Toronto, Ontario, Canada and to characterize select oseltamivir resistant and susceptible isolates using sequence analysis. Study design Sanger sequencing was used to determine strain type and H275Y mutations based on partial sequencing of the hemagglutinin (HA) (segment 4) and NA genes. Mutations in the NS1 gene (segment 8) were determined by Sanger sequencing and pyrosequencing. Statistical analysis of demographics and proportions of H275 and H275Y isolates with mutations was carried out using χ2 analyses. Results The HA gene of influenza virus A (H1N1) isolates collected during the 2007–2008 respiratory season was most like influenza A/Brisbane/59/2007, Clade 2, subclade B. Seventeen percent of these isolates possessed the H275Y NA mutation associated with oseltamivir resistance. H275Y isolates were more likely than H275 isolates to have the mutations A209T and R224G in NS1 ( χ2 = 284.9, df = 2, p < 0.0001). Conclusions During the 2007–2008 influenza season in Toronto, Ontario, Canada, 17% of influenza virus A (H1N1) isolates carried the H275Y mutation associated with oseltamivir resistance. These H275Y isolates were more likely than H275 isolates to exhibit unique microheterogeneity in the gene encoding the NS1 protein.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19451021</pmid><doi>10.1016/j.jcv.2009.04.010</doi><tpages>4</tpages></addata></record> |
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subjects | Adolescent Adult Aged Aged, 80 and over Allergy and Immunology Amino Acid Substitution - genetics Antiviral Agents - pharmacology Biological and medical sciences Child Child, Preschool Drug Resistance, Viral Female Fundamental and applied biological sciences. Psychology Genetics H275Y Human viral diseases Humans Infant Infectious Disease Infectious diseases Influenza Influenza A virus Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - isolation & purification Influenza, Human - epidemiology Influenza, Human - virology Male Medical sciences Microbiology Middle Aged Miscellaneous Molecular Sequence Data Mutation, Missense Neuraminidase - genetics Ontario - epidemiology Oseltamivir Oseltamivir - pharmacology Polymorphism, Genetic Pyrosequencing Resistance Sequence Analysis, DNA Viral diseases Viral Proteins - genetics Virology Young Adult |
title | Genetic microheterogeneity of emerging H275Y influenza virus A (H1N1) in Toronto, Ontario, Canada from the 2007–2008 respiratory season |
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