H9c2 cardiac muscle cells express all somatostatin receptor subtypes

The aim of the present study was to verify the hypothesis that SS receptor subtypes (SSTRs) are expressed by H9c2 cardiac muscle cells. SSTRs expression was investigated by RT-PCR and Western blot analysis at both mRNA and protein level. Our findings demonstrate that H9c2 cells express all SSTR subt...

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Bibliographic Details
Published in:Journal of endocrinological investigation 2004-10, Vol.27 (9), p.RC24-27
Main Authors: Granata, R, Trovato, L, Destefanis, S, Settanni, F, Ghigo, E
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Cell Line
Embryo, Mammalian
Fluorescent Antibody Technique
Myocytes, Cardiac - metabolism
Protein Isoforms - genetics
Protein Isoforms - metabolism
Rats
Receptors, Somatostatin - genetics
Receptors, Somatostatin - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Staining and Labeling
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Summary:The aim of the present study was to verify the hypothesis that SS receptor subtypes (SSTRs) are expressed by H9c2 cardiac muscle cells. SSTRs expression was investigated by RT-PCR and Western blot analysis at both mRNA and protein level. Our findings demonstrate that H9c2 cells express all SSTR subtypes I-5 (SSTRI-5) at the mRNA and protein level. Thus, H9c2 cells would represent a new model to study the direct biological activities of SS and its analogues at the cardiac level.
ISSN:0391-4097
1720-8386
DOI:10.1007/bf03346272