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Mantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case report
Background: Plasmacytic differentiation in mantle cell lymphoma (MCL) occurs rarely. However, no flow cytometric studies that demonstrate plasmacytic (PC) differentiation in MCL have been reported. Herein, we report a case of MCL with PC differentiation identified by flow cytometry. Methods: Morphol...
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| Published in: | Cytometry. Part B, Clinical cytometry Clinical cytometry, 2009-05, Vol.76B (3), p.218-224 |
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| container_title | Cytometry. Part B, Clinical cytometry |
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| creator | Naushad, Hina Choi, William W. L. Page, Cynthia J. Sanger, Warren G. Weisenburger, Dennis D. Aoun, Patricia |
| description | Background:
Plasmacytic differentiation in mantle cell lymphoma (MCL) occurs rarely. However, no flow cytometric studies that demonstrate plasmacytic (PC) differentiation in MCL have been reported. Herein, we report a case of MCL with PC differentiation identified by flow cytometry.
Methods:
Morphologic review was performed by hematoxylin and eosin (H&E) stained sections from paraffin‐embedded lymph node, colon and bone marrow specimens, and Wright‐Geimsa stained bone marrow aspirate smears and touch imprints. Immunohistochemical stains using antibodies against CD3, CD5, CD20, and cyclin‐D1, and in‐situ hybridization for kappa and lambda light chains were reviewed. Multicolor flow cytometry analysis was performed on the bone marrow aspirate with monoclonal antibodies to CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD23, CD38, CD45, CD56, CD138, and kappa and lambda light chains. FISH analysis for t(11;14)(q13;q32) was performed on interphase cells.
Results:
The neoplastic cells had the cytologic features of MCL with nodal, bone marrow, and colonic involvement. In‐situ hybridization for kappa and lambda light chains demonstrated clonal plasma cells in the lymph node and bone marrow biopsies. In addition, flow cytometric studies of the bone marrow aspirate showed three populations of neoplastic cells: a clonal B‐cell population with typical MCL phenotype, a similar B‐cell population in transition to plasma cells, and a clonal plasma cell population. The plasma cells retained CD5 expression and had the same light chain restriction as the clonal B‐cells.
Conclusions:
Multi‐parameter flow cytometry can be useful in demonstrating clonal PC differentiation in MCL and distinguishing from a concurrent but unrelated plasma cell dyscrasia. © 2008 Clinical Cytometry Society |
| doi_str_mv | 10.1002/cyto.b.20463 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67125740</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67125740</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4023-1dc300a8f5a9a4694af31d80d3f252f851195766b5514e346bc0d7ae3cca01e53</originalsourceid><addsrcrecordid>eNqFkT1v2zAQhokiRZO43ToXnDLV7vFTUrfAaD6AFFnSoRNBUceGBSWqpBzD_75ybDRbM90B9-AB3nsJ-chgxQD4F7eb0qpdcZBavCFnTCm-lI2qTv7tsjkl56X8BhBK6uodOWUNaMZlc0Z-fbfDFJE6jJHGXT8-pt7SbZgeqY9pS_f2HqccHMWn0OHgkCZPXUyDjXSMtvR2ZuZzF7zHjMMU7BTS8JVeUmcL0oxjytN78tbbWPDDcS7Ij6tvD-ub5d399e368m7pJHCxZJ0TALb2yjZW6kZaL1hXQyc8V9zXirE5mtatUkyikLp10FUWhXMWGCqxIBcH75jTnw2WyfSh7MPZAdOmGF0xrioJr4IcaqhroWfw8wF0OZWS0Zsxh97mnWFg9g2Y_Y9Ma54bmPFPR--m7bF7gY8vnwFxALYh4u6_MrP--XB_0P4FzjmTaQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20808836</pqid></control><display><type>article</type><title>Mantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case report</title><source>Wiley-Blackwell Journals</source><creator>Naushad, Hina ; Choi, William W. L. ; Page, Cynthia J. ; Sanger, Warren G. ; Weisenburger, Dennis D. ; Aoun, Patricia</creator><creatorcontrib>Naushad, Hina ; Choi, William W. L. ; Page, Cynthia J. ; Sanger, Warren G. ; Weisenburger, Dennis D. ; Aoun, Patricia</creatorcontrib><description>Background:
Plasmacytic differentiation in mantle cell lymphoma (MCL) occurs rarely. However, no flow cytometric studies that demonstrate plasmacytic (PC) differentiation in MCL have been reported. Herein, we report a case of MCL with PC differentiation identified by flow cytometry.
Methods:
Morphologic review was performed by hematoxylin and eosin (H&E) stained sections from paraffin‐embedded lymph node, colon and bone marrow specimens, and Wright‐Geimsa stained bone marrow aspirate smears and touch imprints. Immunohistochemical stains using antibodies against CD3, CD5, CD20, and cyclin‐D1, and in‐situ hybridization for kappa and lambda light chains were reviewed. Multicolor flow cytometry analysis was performed on the bone marrow aspirate with monoclonal antibodies to CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD23, CD38, CD45, CD56, CD138, and kappa and lambda light chains. FISH analysis for t(11;14)(q13;q32) was performed on interphase cells.
Results:
The neoplastic cells had the cytologic features of MCL with nodal, bone marrow, and colonic involvement. In‐situ hybridization for kappa and lambda light chains demonstrated clonal plasma cells in the lymph node and bone marrow biopsies. In addition, flow cytometric studies of the bone marrow aspirate showed three populations of neoplastic cells: a clonal B‐cell population with typical MCL phenotype, a similar B‐cell population in transition to plasma cells, and a clonal plasma cell population. The plasma cells retained CD5 expression and had the same light chain restriction as the clonal B‐cells.
Conclusions:
Multi‐parameter flow cytometry can be useful in demonstrating clonal PC differentiation in MCL and distinguishing from a concurrent but unrelated plasma cell dyscrasia. © 2008 Clinical Cytometry Society</description><identifier>ISSN: 1552-4949</identifier><identifier>EISSN: 1552-4957</identifier><identifier>DOI: 10.1002/cyto.b.20463</identifier><identifier>PMID: 19061249</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Aged ; CD5+ plasma cells ; Cell Differentiation ; Clone Cells - pathology ; Flow Cytometry ; Humans ; Immunohistochemistry ; Lymphoma, Mantle-Cell - pathology ; Male ; mantle cell lymphoma ; Plasma Cells - pathology ; plasmacytic differentiation ; Sensitivity and Specificity</subject><ispartof>Cytometry. Part B, Clinical cytometry, 2009-05, Vol.76B (3), p.218-224</ispartof><rights>Copyright © 2008 Clinical Cytometry Society</rights><rights>(c) 2008 Clinical Cytometry Society.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4023-1dc300a8f5a9a4694af31d80d3f252f851195766b5514e346bc0d7ae3cca01e53</citedby><cites>FETCH-LOGICAL-c4023-1dc300a8f5a9a4694af31d80d3f252f851195766b5514e346bc0d7ae3cca01e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcyto.b.20463$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcyto.b.20463$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19061249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naushad, Hina</creatorcontrib><creatorcontrib>Choi, William W. L.</creatorcontrib><creatorcontrib>Page, Cynthia J.</creatorcontrib><creatorcontrib>Sanger, Warren G.</creatorcontrib><creatorcontrib>Weisenburger, Dennis D.</creatorcontrib><creatorcontrib>Aoun, Patricia</creatorcontrib><title>Mantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case report</title><title>Cytometry. Part B, Clinical cytometry</title><addtitle>Cytometry B Clin Cytom</addtitle><description>Background:
Plasmacytic differentiation in mantle cell lymphoma (MCL) occurs rarely. However, no flow cytometric studies that demonstrate plasmacytic (PC) differentiation in MCL have been reported. Herein, we report a case of MCL with PC differentiation identified by flow cytometry.
Methods:
Morphologic review was performed by hematoxylin and eosin (H&E) stained sections from paraffin‐embedded lymph node, colon and bone marrow specimens, and Wright‐Geimsa stained bone marrow aspirate smears and touch imprints. Immunohistochemical stains using antibodies against CD3, CD5, CD20, and cyclin‐D1, and in‐situ hybridization for kappa and lambda light chains were reviewed. Multicolor flow cytometry analysis was performed on the bone marrow aspirate with monoclonal antibodies to CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD23, CD38, CD45, CD56, CD138, and kappa and lambda light chains. FISH analysis for t(11;14)(q13;q32) was performed on interphase cells.
Results:
The neoplastic cells had the cytologic features of MCL with nodal, bone marrow, and colonic involvement. In‐situ hybridization for kappa and lambda light chains demonstrated clonal plasma cells in the lymph node and bone marrow biopsies. In addition, flow cytometric studies of the bone marrow aspirate showed three populations of neoplastic cells: a clonal B‐cell population with typical MCL phenotype, a similar B‐cell population in transition to plasma cells, and a clonal plasma cell population. The plasma cells retained CD5 expression and had the same light chain restriction as the clonal B‐cells.
Conclusions:
Multi‐parameter flow cytometry can be useful in demonstrating clonal PC differentiation in MCL and distinguishing from a concurrent but unrelated plasma cell dyscrasia. © 2008 Clinical Cytometry Society</description><subject>Aged</subject><subject>CD5+ plasma cells</subject><subject>Cell Differentiation</subject><subject>Clone Cells - pathology</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Lymphoma, Mantle-Cell - pathology</subject><subject>Male</subject><subject>mantle cell lymphoma</subject><subject>Plasma Cells - pathology</subject><subject>plasmacytic differentiation</subject><subject>Sensitivity and Specificity</subject><issn>1552-4949</issn><issn>1552-4957</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkT1v2zAQhokiRZO43ToXnDLV7vFTUrfAaD6AFFnSoRNBUceGBSWqpBzD_75ybDRbM90B9-AB3nsJ-chgxQD4F7eb0qpdcZBavCFnTCm-lI2qTv7tsjkl56X8BhBK6uodOWUNaMZlc0Z-fbfDFJE6jJHGXT8-pt7SbZgeqY9pS_f2HqccHMWn0OHgkCZPXUyDjXSMtvR2ZuZzF7zHjMMU7BTS8JVeUmcL0oxjytN78tbbWPDDcS7Ij6tvD-ub5d399e368m7pJHCxZJ0TALb2yjZW6kZaL1hXQyc8V9zXirE5mtatUkyikLp10FUWhXMWGCqxIBcH75jTnw2WyfSh7MPZAdOmGF0xrioJr4IcaqhroWfw8wF0OZWS0Zsxh97mnWFg9g2Y_Y9Ma54bmPFPR--m7bF7gY8vnwFxALYh4u6_MrP--XB_0P4FzjmTaQ</recordid><startdate>200905</startdate><enddate>200905</enddate><creator>Naushad, Hina</creator><creator>Choi, William W. L.</creator><creator>Page, Cynthia J.</creator><creator>Sanger, Warren G.</creator><creator>Weisenburger, Dennis D.</creator><creator>Aoun, Patricia</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200905</creationdate><title>Mantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case report</title><author>Naushad, Hina ; Choi, William W. L. ; Page, Cynthia J. ; Sanger, Warren G. ; Weisenburger, Dennis D. ; Aoun, Patricia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4023-1dc300a8f5a9a4694af31d80d3f252f851195766b5514e346bc0d7ae3cca01e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Aged</topic><topic>CD5+ plasma cells</topic><topic>Cell Differentiation</topic><topic>Clone Cells - pathology</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Lymphoma, Mantle-Cell - pathology</topic><topic>Male</topic><topic>mantle cell lymphoma</topic><topic>Plasma Cells - pathology</topic><topic>plasmacytic differentiation</topic><topic>Sensitivity and Specificity</topic><toplevel>online_resources</toplevel><creatorcontrib>Naushad, Hina</creatorcontrib><creatorcontrib>Choi, William W. L.</creatorcontrib><creatorcontrib>Page, Cynthia J.</creatorcontrib><creatorcontrib>Sanger, Warren G.</creatorcontrib><creatorcontrib>Weisenburger, Dennis D.</creatorcontrib><creatorcontrib>Aoun, Patricia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cytometry. Part B, Clinical cytometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naushad, Hina</au><au>Choi, William W. L.</au><au>Page, Cynthia J.</au><au>Sanger, Warren G.</au><au>Weisenburger, Dennis D.</au><au>Aoun, Patricia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case report</atitle><jtitle>Cytometry. Part B, Clinical cytometry</jtitle><addtitle>Cytometry B Clin Cytom</addtitle><date>2009-05</date><risdate>2009</risdate><volume>76B</volume><issue>3</issue><spage>218</spage><epage>224</epage><pages>218-224</pages><issn>1552-4949</issn><eissn>1552-4957</eissn><notes>How to cite this article: Naushad H, Choi WWL, Page CJ, Sanger WG, Weisenburger DD, Aoun P. Mantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case report. Cytometry Part B 2009; 76B: 218–224.</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>ObjectType-Case Study-2</notes><notes>ObjectType-Feature-4</notes><notes>ObjectType-Report-1</notes><notes>ObjectType-Article-3</notes><abstract>Background:
Plasmacytic differentiation in mantle cell lymphoma (MCL) occurs rarely. However, no flow cytometric studies that demonstrate plasmacytic (PC) differentiation in MCL have been reported. Herein, we report a case of MCL with PC differentiation identified by flow cytometry.
Methods:
Morphologic review was performed by hematoxylin and eosin (H&E) stained sections from paraffin‐embedded lymph node, colon and bone marrow specimens, and Wright‐Geimsa stained bone marrow aspirate smears and touch imprints. Immunohistochemical stains using antibodies against CD3, CD5, CD20, and cyclin‐D1, and in‐situ hybridization for kappa and lambda light chains were reviewed. Multicolor flow cytometry analysis was performed on the bone marrow aspirate with monoclonal antibodies to CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD23, CD38, CD45, CD56, CD138, and kappa and lambda light chains. FISH analysis for t(11;14)(q13;q32) was performed on interphase cells.
Results:
The neoplastic cells had the cytologic features of MCL with nodal, bone marrow, and colonic involvement. In‐situ hybridization for kappa and lambda light chains demonstrated clonal plasma cells in the lymph node and bone marrow biopsies. In addition, flow cytometric studies of the bone marrow aspirate showed three populations of neoplastic cells: a clonal B‐cell population with typical MCL phenotype, a similar B‐cell population in transition to plasma cells, and a clonal plasma cell population. The plasma cells retained CD5 expression and had the same light chain restriction as the clonal B‐cells.
Conclusions:
Multi‐parameter flow cytometry can be useful in demonstrating clonal PC differentiation in MCL and distinguishing from a concurrent but unrelated plasma cell dyscrasia. © 2008 Clinical Cytometry Society</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>19061249</pmid><doi>10.1002/cyto.b.20463</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cytometry. Part B, Clinical cytometry, 2009-05, Vol.76B (3), p.218-224 |
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| language | eng |
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| source | Wiley-Blackwell Journals |
| subjects | Aged CD5+ plasma cells Cell Differentiation Clone Cells - pathology Flow Cytometry Humans Immunohistochemistry Lymphoma, Mantle-Cell - pathology Male mantle cell lymphoma Plasma Cells - pathology plasmacytic differentiation Sensitivity and Specificity |
| title | Mantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case report |
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