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Relationships Between Local and Systemic Expression of Interleukin-12 and Plasma Levels of Vascular Endothelial Growth Factor in Patients with Gastric Cancer
Background: Interleukin 12 (IL-12) is a heterodimeric cytokine that exhibits potent anti-tumor and anti-metastatic activities. Very few studies have investigated the expression of IL-12 in patients with gastric cancer. The purpose of the present study was to evaluate the immunohistochemical expressi...
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| Published in: | Anticancer research 2004-09, Vol.24 (5C), p.3289-3294 |
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| creator | NAKAYAMA, Yoshifumi INOUE, Yuzuru ITOH, Hideaki NAGASHIMA, Nobuo KATSUKI, Takefumi MATSUMOTO, Kentarou SHIBAO, Kazunori TSURUDOME, Keisuke HIRATA, Keiji SAKO, Tatsuhiko NAGATA, Naoki |
| description | Background: Interleukin 12 (IL-12) is a heterodimeric cytokine that exhibits potent anti-tumor and anti-metastatic activities.
Very few studies have investigated the expression of IL-12 in patients with gastric cancer. The purpose of the present study
was to evaluate the immunohistochemical expression and serum levels of IL-12, and plasma levels of vascular endothelial growth
factor (VEGF) in patients with gastric cancer. Patients and Methods: IL-12 was immunohistochemically stained using monoclonal
anti-human IL-12 antibody (1-1A4) in surgical specimens of 27 gastric cancer patients. IL-12-positive cells were counted and
positive cell density was calculated. Blood was obtained before surgery. Serum levels of IL-12 and plasma levels of VEGF were
assessed using the quantitative sandwich enzyme immunoassay technique. The relationships of IL-12-positive cell density, serum
levels of IL-12, plasma levels of vascular endothelial growth factor (VEGF) and clinicopathological factors were evaluated.
Results: Although IL-12-positive cell density was not associated with serum levels of IL-12, the IL-12-positive cell density
tended to increase in patients with serum levels of IL-12 higher than the mean level (p=0.0518). IL-12-positive cell density
significantly decreased in the patients with CEA positive or differentiated type. Moreover, IL-12-positive cell density tended
to be inversely correlated with plasma levels of VEGF (p=0.0801, r=-0.343). Conclusion: These results suggest that the immune
efficiency in patients with gastric cancer may be reflected by IL-12-positive cell density and serum levels of IL-12. Thus,
patients with low IL-12-positive cell density or serum levels of IL-12 in gastric cancer may require additional immunochemotherapy
after surgery. |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_67024075</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67024075</sourcerecordid><originalsourceid>FETCH-LOGICAL-h299t-588cb910429caa8fba02647849b06aebbd188687bae9b0456ccbf728777a54213</originalsourceid><addsrcrecordid>eNqFkMFu1DAURSNURIfCL1Te0F0k24lje0lH06HSSIwo7TZ68bwQF8cZbKdDP4Z_xYWpuuzqSVdH9-qdN8WCSc1KKSp6UiwoF7SUlIrT4n2M95Q2jVbVu-KUCcFEzatF8ecbOkh28nGw-0guMR0QPdlMBhwBvyM3jzHhaA1Z_d4HjDGjZOrJtU8YHM4_rS8Z_0duHcQRyAYf0MUn5g6imR0EsvK7KQ3obO5ch-mQBnIFJk2BWE-2eR59iuRgc76GmEJeW4I3GD4Ub3twET8e71lxe7X6vvxSbr6ur5efN-XAtU6lUMp0mtGaawOg-g4ob2qpat3RBrDrdkypRskOMCe1aIzpesmVlBKyBladFRf_e_dh-jVjTO1oo0HnwOM0x7aRlNc0W30NZJJrIeVT4_kRnLsRd-0-2BHCY_tsPgOfjkC2BK4P-WEbX7iGU61l9bI42B_DwQZss2Tncm3VQuB1K5ZtxZWu_gKCuZv7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17295771</pqid></control><display><type>article</type><title>Relationships Between Local and Systemic Expression of Interleukin-12 and Plasma Levels of Vascular Endothelial Growth Factor in Patients with Gastric Cancer</title><source>EZB Electronic Journals Library</source><creator>NAKAYAMA, Yoshifumi ; INOUE, Yuzuru ; ITOH, Hideaki ; NAGASHIMA, Nobuo ; KATSUKI, Takefumi ; MATSUMOTO, Kentarou ; SHIBAO, Kazunori ; TSURUDOME, Keisuke ; HIRATA, Keiji ; SAKO, Tatsuhiko ; NAGATA, Naoki</creator><creatorcontrib>NAKAYAMA, Yoshifumi ; INOUE, Yuzuru ; ITOH, Hideaki ; NAGASHIMA, Nobuo ; KATSUKI, Takefumi ; MATSUMOTO, Kentarou ; SHIBAO, Kazunori ; TSURUDOME, Keisuke ; HIRATA, Keiji ; SAKO, Tatsuhiko ; NAGATA, Naoki</creatorcontrib><description>Background: Interleukin 12 (IL-12) is a heterodimeric cytokine that exhibits potent anti-tumor and anti-metastatic activities.
Very few studies have investigated the expression of IL-12 in patients with gastric cancer. The purpose of the present study
was to evaluate the immunohistochemical expression and serum levels of IL-12, and plasma levels of vascular endothelial growth
factor (VEGF) in patients with gastric cancer. Patients and Methods: IL-12 was immunohistochemically stained using monoclonal
anti-human IL-12 antibody (1-1A4) in surgical specimens of 27 gastric cancer patients. IL-12-positive cells were counted and
positive cell density was calculated. Blood was obtained before surgery. Serum levels of IL-12 and plasma levels of VEGF were
assessed using the quantitative sandwich enzyme immunoassay technique. The relationships of IL-12-positive cell density, serum
levels of IL-12, plasma levels of vascular endothelial growth factor (VEGF) and clinicopathological factors were evaluated.
Results: Although IL-12-positive cell density was not associated with serum levels of IL-12, the IL-12-positive cell density
tended to increase in patients with serum levels of IL-12 higher than the mean level (p=0.0518). IL-12-positive cell density
significantly decreased in the patients with CEA positive or differentiated type. Moreover, IL-12-positive cell density tended
to be inversely correlated with plasma levels of VEGF (p=0.0801, r=-0.343). Conclusion: These results suggest that the immune
efficiency in patients with gastric cancer may be reflected by IL-12-positive cell density and serum levels of IL-12. Thus,
patients with low IL-12-positive cell density or serum levels of IL-12 in gastric cancer may require additional immunochemotherapy
after surgery.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 15515423</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Aged ; Aged, 80 and over ; Biological and medical sciences ; Cell Count ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Immunohistochemistry ; Interleukin-12 - blood ; Interleukin-12 - metabolism ; Male ; Medical sciences ; Middle Aged ; Neoplasm Staging ; Stomach Neoplasms - blood ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Tumors ; Vascular Endothelial Growth Factor A - blood</subject><ispartof>Anticancer research, 2004-09, Vol.24 (5C), p.3289-3294</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>310,311,315,786,790,795,796,23958,23959,25170</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16209973$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15515423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAKAYAMA, Yoshifumi</creatorcontrib><creatorcontrib>INOUE, Yuzuru</creatorcontrib><creatorcontrib>ITOH, Hideaki</creatorcontrib><creatorcontrib>NAGASHIMA, Nobuo</creatorcontrib><creatorcontrib>KATSUKI, Takefumi</creatorcontrib><creatorcontrib>MATSUMOTO, Kentarou</creatorcontrib><creatorcontrib>SHIBAO, Kazunori</creatorcontrib><creatorcontrib>TSURUDOME, Keisuke</creatorcontrib><creatorcontrib>HIRATA, Keiji</creatorcontrib><creatorcontrib>SAKO, Tatsuhiko</creatorcontrib><creatorcontrib>NAGATA, Naoki</creatorcontrib><title>Relationships Between Local and Systemic Expression of Interleukin-12 and Plasma Levels of Vascular Endothelial Growth Factor in Patients with Gastric Cancer</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: Interleukin 12 (IL-12) is a heterodimeric cytokine that exhibits potent anti-tumor and anti-metastatic activities.
Very few studies have investigated the expression of IL-12 in patients with gastric cancer. The purpose of the present study
was to evaluate the immunohistochemical expression and serum levels of IL-12, and plasma levels of vascular endothelial growth
factor (VEGF) in patients with gastric cancer. Patients and Methods: IL-12 was immunohistochemically stained using monoclonal
anti-human IL-12 antibody (1-1A4) in surgical specimens of 27 gastric cancer patients. IL-12-positive cells were counted and
positive cell density was calculated. Blood was obtained before surgery. Serum levels of IL-12 and plasma levels of VEGF were
assessed using the quantitative sandwich enzyme immunoassay technique. The relationships of IL-12-positive cell density, serum
levels of IL-12, plasma levels of vascular endothelial growth factor (VEGF) and clinicopathological factors were evaluated.
Results: Although IL-12-positive cell density was not associated with serum levels of IL-12, the IL-12-positive cell density
tended to increase in patients with serum levels of IL-12 higher than the mean level (p=0.0518). IL-12-positive cell density
significantly decreased in the patients with CEA positive or differentiated type. Moreover, IL-12-positive cell density tended
to be inversely correlated with plasma levels of VEGF (p=0.0801, r=-0.343). Conclusion: These results suggest that the immune
efficiency in patients with gastric cancer may be reflected by IL-12-positive cell density and serum levels of IL-12. Thus,
patients with low IL-12-positive cell density or serum levels of IL-12 in gastric cancer may require additional immunochemotherapy
after surgery.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Interleukin-12 - blood</subject><subject>Interleukin-12 - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Staging</subject><subject>Stomach Neoplasms - blood</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Tumors</subject><subject>Vascular Endothelial Growth Factor A - blood</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkMFu1DAURSNURIfCL1Te0F0k24lje0lH06HSSIwo7TZ68bwQF8cZbKdDP4Z_xYWpuuzqSVdH9-qdN8WCSc1KKSp6UiwoF7SUlIrT4n2M95Q2jVbVu-KUCcFEzatF8ecbOkh28nGw-0guMR0QPdlMBhwBvyM3jzHhaA1Z_d4HjDGjZOrJtU8YHM4_rS8Z_0duHcQRyAYf0MUn5g6imR0EsvK7KQ3obO5ch-mQBnIFJk2BWE-2eR59iuRgc76GmEJeW4I3GD4Ub3twET8e71lxe7X6vvxSbr6ur5efN-XAtU6lUMp0mtGaawOg-g4ob2qpat3RBrDrdkypRskOMCe1aIzpesmVlBKyBladFRf_e_dh-jVjTO1oo0HnwOM0x7aRlNc0W30NZJJrIeVT4_kRnLsRd-0-2BHCY_tsPgOfjkC2BK4P-WEbX7iGU61l9bI42B_DwQZss2Tncm3VQuB1K5ZtxZWu_gKCuZv7</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>NAKAYAMA, Yoshifumi</creator><creator>INOUE, Yuzuru</creator><creator>ITOH, Hideaki</creator><creator>NAGASHIMA, Nobuo</creator><creator>KATSUKI, Takefumi</creator><creator>MATSUMOTO, Kentarou</creator><creator>SHIBAO, Kazunori</creator><creator>TSURUDOME, Keisuke</creator><creator>HIRATA, Keiji</creator><creator>SAKO, Tatsuhiko</creator><creator>NAGATA, Naoki</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>Relationships Between Local and Systemic Expression of Interleukin-12 and Plasma Levels of Vascular Endothelial Growth Factor in Patients with Gastric Cancer</title><author>NAKAYAMA, Yoshifumi ; INOUE, Yuzuru ; ITOH, Hideaki ; NAGASHIMA, Nobuo ; KATSUKI, Takefumi ; MATSUMOTO, Kentarou ; SHIBAO, Kazunori ; TSURUDOME, Keisuke ; HIRATA, Keiji ; SAKO, Tatsuhiko ; NAGATA, Naoki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h299t-588cb910429caa8fba02647849b06aebbd188687bae9b0456ccbf728777a54213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Interleukin-12 - blood</topic><topic>Interleukin-12 - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasm Staging</topic><topic>Stomach Neoplasms - blood</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Tumors</topic><topic>Vascular Endothelial Growth Factor A - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NAKAYAMA, Yoshifumi</creatorcontrib><creatorcontrib>INOUE, Yuzuru</creatorcontrib><creatorcontrib>ITOH, Hideaki</creatorcontrib><creatorcontrib>NAGASHIMA, Nobuo</creatorcontrib><creatorcontrib>KATSUKI, Takefumi</creatorcontrib><creatorcontrib>MATSUMOTO, Kentarou</creatorcontrib><creatorcontrib>SHIBAO, Kazunori</creatorcontrib><creatorcontrib>TSURUDOME, Keisuke</creatorcontrib><creatorcontrib>HIRATA, Keiji</creatorcontrib><creatorcontrib>SAKO, Tatsuhiko</creatorcontrib><creatorcontrib>NAGATA, Naoki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NAKAYAMA, Yoshifumi</au><au>INOUE, Yuzuru</au><au>ITOH, Hideaki</au><au>NAGASHIMA, Nobuo</au><au>KATSUKI, Takefumi</au><au>MATSUMOTO, Kentarou</au><au>SHIBAO, Kazunori</au><au>TSURUDOME, Keisuke</au><au>HIRATA, Keiji</au><au>SAKO, Tatsuhiko</au><au>NAGATA, Naoki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationships Between Local and Systemic Expression of Interleukin-12 and Plasma Levels of Vascular Endothelial Growth Factor in Patients with Gastric Cancer</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>24</volume><issue>5C</issue><spage>3289</spage><epage>3294</epage><pages>3289-3294</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><abstract>Background: Interleukin 12 (IL-12) is a heterodimeric cytokine that exhibits potent anti-tumor and anti-metastatic activities.
Very few studies have investigated the expression of IL-12 in patients with gastric cancer. The purpose of the present study
was to evaluate the immunohistochemical expression and serum levels of IL-12, and plasma levels of vascular endothelial growth
factor (VEGF) in patients with gastric cancer. Patients and Methods: IL-12 was immunohistochemically stained using monoclonal
anti-human IL-12 antibody (1-1A4) in surgical specimens of 27 gastric cancer patients. IL-12-positive cells were counted and
positive cell density was calculated. Blood was obtained before surgery. Serum levels of IL-12 and plasma levels of VEGF were
assessed using the quantitative sandwich enzyme immunoassay technique. The relationships of IL-12-positive cell density, serum
levels of IL-12, plasma levels of vascular endothelial growth factor (VEGF) and clinicopathological factors were evaluated.
Results: Although IL-12-positive cell density was not associated with serum levels of IL-12, the IL-12-positive cell density
tended to increase in patients with serum levels of IL-12 higher than the mean level (p=0.0518). IL-12-positive cell density
significantly decreased in the patients with CEA positive or differentiated type. Moreover, IL-12-positive cell density tended
to be inversely correlated with plasma levels of VEGF (p=0.0801, r=-0.343). Conclusion: These results suggest that the immune
efficiency in patients with gastric cancer may be reflected by IL-12-positive cell density and serum levels of IL-12. Thus,
patients with low IL-12-positive cell density or serum levels of IL-12 in gastric cancer may require additional immunochemotherapy
after surgery.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>15515423</pmid><tpages>6</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Biological and medical sciences Cell Count Female Gastroenterology. Liver. Pancreas. Abdomen Humans Immunohistochemistry Interleukin-12 - blood Interleukin-12 - metabolism Male Medical sciences Middle Aged Neoplasm Staging Stomach Neoplasms - blood Stomach Neoplasms - metabolism Stomach Neoplasms - pathology Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumors Vascular Endothelial Growth Factor A - blood |
| title | Relationships Between Local and Systemic Expression of Interleukin-12 and Plasma Levels of Vascular Endothelial Growth Factor in Patients with Gastric Cancer |
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