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In vitro–matured rat oocytes have low mitochondrial deoxyribonucleic acid and adenosine triphosphate contents and have abnormal mitochondrial redistribution
Objective To study the development and function of mitochondria in in vitro–matured rat oocytes derived from follicles of different sizes. Design Experimental animal study. Setting Department of Anatomy at the University of Hong Kong. Animal(s) Immature female Sprague-Dawley rats that were 25 days o...
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| Published in: | Fertility and sterility 2009-03, Vol.91 (3), p.900-907 |
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| container_title | Fertility and sterility |
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| creator | Zeng, Hai-tao, M.D., Ph.D Yeung, William S.B., Ph.D Cheung, May P.L., B.Sc Ho, Pak-Chung, M.D Lee, Calvin K.F., Ph.D Zhuang, Guang-lun, M.D Liang, Xiao-yan, Ph.D O, Wai-Sum, Ph.D |
| description | Objective To study the development and function of mitochondria in in vitro–matured rat oocytes derived from follicles of different sizes. Design Experimental animal study. Setting Department of Anatomy at the University of Hong Kong. Animal(s) Immature female Sprague-Dawley rats that were 25 days of age. Intervention(s) Immature oocytes were collected from rat ovarian follicles of different sizes and were induced to mature in vitro. Main Outcome Measure(s) The number of copies of mitochondrial DNA, mitochondrial activity, adenosine triphosphate content of matured oocytes, and rates of fertilization and blastulation were determined. Result(s) The mitochondrial DNA copy number of oocytes increased linearly with the diameter of antral follicles. The mitochondrial DNA copy number, adenosine triphosphate content, and proportion of oocytes with peripheral distribution of mitochondria in in vitro–matured oocytes from small antral follicles were significantly lower than those from preovulatory follicles and in vivo–matured oocytes. Compared with in vitro–matured oocytes from small antral follicles, those from preovulatory follicles and in vivo–matured oocytes also had significantly better fertilization potential and higher blastulation rate. Conclusion(s) The inferior developmental potential of in vitro–matured oocytes may be attributed partly to a reduced number of mitochondria, resulting in insufficient production of adenosine triphosphate for required developmental events. |
| doi_str_mv | 10.1016/j.fertnstert.2007.12.008 |
| format | article |
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Design Experimental animal study. Setting Department of Anatomy at the University of Hong Kong. Animal(s) Immature female Sprague-Dawley rats that were 25 days of age. Intervention(s) Immature oocytes were collected from rat ovarian follicles of different sizes and were induced to mature in vitro. Main Outcome Measure(s) The number of copies of mitochondrial DNA, mitochondrial activity, adenosine triphosphate content of matured oocytes, and rates of fertilization and blastulation were determined. Result(s) The mitochondrial DNA copy number of oocytes increased linearly with the diameter of antral follicles. The mitochondrial DNA copy number, adenosine triphosphate content, and proportion of oocytes with peripheral distribution of mitochondria in in vitro–matured oocytes from small antral follicles were significantly lower than those from preovulatory follicles and in vivo–matured oocytes. Compared with in vitro–matured oocytes from small antral follicles, those from preovulatory follicles and in vivo–matured oocytes also had significantly better fertilization potential and higher blastulation rate. Conclusion(s) The inferior developmental potential of in vitro–matured oocytes may be attributed partly to a reduced number of mitochondria, resulting in insufficient production of adenosine triphosphate for required developmental events.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2007.12.008</identifier><identifier>PMID: 18321496</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; Biological and medical sciences ; Blastula - metabolism ; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology ; Cells, Cultured ; development ; DNA, Mitochondrial - metabolism ; Embryo Culture Techniques ; Embryonic Development ; Energy Metabolism ; Female ; Fertilization in Vitro ; Gynecology. Andrology. Obstetrics ; in vitro maturation ; Internal Medicine ; Medical sciences ; Mitochondria ; Mitochondria - drug effects ; Mitochondria - metabolism ; Obstetrics and Gynecology ; oocyte ; Oocytes - drug effects ; Oocytes - metabolism ; Rats ; Rats, Sprague-Dawley ; Uncoupling Agents - pharmacology</subject><ispartof>Fertility and sterility, 2009-03, Vol.91 (3), p.900-907</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2009 American Society for Reproductive Medicine</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-622f342c365f3db2e5a905e537732501e53bd9c409df7ecd09cc6deb4a40cddc3</citedby><cites>FETCH-LOGICAL-c573t-622f342c365f3db2e5a905e537732501e53bd9c409df7ecd09cc6deb4a40cddc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0015028207041908$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,786,790,3568,27957,27958,45815</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21244606$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18321496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Hai-tao, M.D., Ph.D</creatorcontrib><creatorcontrib>Yeung, William S.B., Ph.D</creatorcontrib><creatorcontrib>Cheung, May P.L., B.Sc</creatorcontrib><creatorcontrib>Ho, Pak-Chung, M.D</creatorcontrib><creatorcontrib>Lee, Calvin K.F., Ph.D</creatorcontrib><creatorcontrib>Zhuang, Guang-lun, M.D</creatorcontrib><creatorcontrib>Liang, Xiao-yan, Ph.D</creatorcontrib><creatorcontrib>O, Wai-Sum, Ph.D</creatorcontrib><title>In vitro–matured rat oocytes have low mitochondrial deoxyribonucleic acid and adenosine triphosphate contents and have abnormal mitochondrial redistribution</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective To study the development and function of mitochondria in in vitro–matured rat oocytes derived from follicles of different sizes. Design Experimental animal study. Setting Department of Anatomy at the University of Hong Kong. Animal(s) Immature female Sprague-Dawley rats that were 25 days of age. Intervention(s) Immature oocytes were collected from rat ovarian follicles of different sizes and were induced to mature in vitro. Main Outcome Measure(s) The number of copies of mitochondrial DNA, mitochondrial activity, adenosine triphosphate content of matured oocytes, and rates of fertilization and blastulation were determined. Result(s) The mitochondrial DNA copy number of oocytes increased linearly with the diameter of antral follicles. The mitochondrial DNA copy number, adenosine triphosphate content, and proportion of oocytes with peripheral distribution of mitochondria in in vitro–matured oocytes from small antral follicles were significantly lower than those from preovulatory follicles and in vivo–matured oocytes. Compared with in vitro–matured oocytes from small antral follicles, those from preovulatory follicles and in vivo–matured oocytes also had significantly better fertilization potential and higher blastulation rate. Conclusion(s) The inferior developmental potential of in vitro–matured oocytes may be attributed partly to a reduced number of mitochondria, resulting in insufficient production of adenosine triphosphate for required developmental events.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastula - metabolism</subject><subject>Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology</subject><subject>Cells, Cultured</subject><subject>development</subject><subject>DNA, Mitochondrial - metabolism</subject><subject>Embryo Culture Techniques</subject><subject>Embryonic Development</subject><subject>Energy Metabolism</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>in vitro maturation</subject><subject>Internal Medicine</subject><subject>Medical sciences</subject><subject>Mitochondria</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Obstetrics and Gynecology</subject><subject>oocyte</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Uncoupling Agents - pharmacology</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNks9u1DAQxiMEokvhFZAvcMtiO7GTXJCgolCpEgfgbDnjidZLYi-2s2VvvAP3PhxPgtNdUcGJg_8cft83o_mmKAija0aZfLVdDxiSiynfa05ps2Z8TWn7oFgxIWQppKgeFitKmSgpb_lZ8STGLaVUsoY_Ls5YW3FWd3JV3F45srcp-F8_fk46zQENCToR7-GQMJKN3iMZ_Q2ZbPKw8c4Eq0di0H8_BNt7N8OIFogGa4h2-Rh0PlqHJAW72_i42-iEBLxL6FK8Y-5Mde98mLLX3865ARuztJ-T9e5p8WjQY8Rnp_e8-HL57vPFh_L64_urizfXJYimSqXkfKhqDpUUQ2V6jkJ3VKComqbigrL8600HNe3M0CAY2gFIg32tawrGQHVevDz67oL_NmNMarIRcBy1Qz9HJWXXtYK3GWyPIAQfY8BB7YKddDgoRtWSjdqq-2zUko1iXOVssvT5qcbcT2juhacwMvDiBOgIehyCdmDjH44zXteSLtzbI4d5InuLQUWw6CBPLiAkZbz9n25e_2MCo3U21_2KB4xbPweXJ66YilmgPi27tKwSbWjNumzwG_r1zmU</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Zeng, Hai-tao, M.D., Ph.D</creator><creator>Yeung, William S.B., Ph.D</creator><creator>Cheung, May P.L., B.Sc</creator><creator>Ho, Pak-Chung, M.D</creator><creator>Lee, Calvin K.F., Ph.D</creator><creator>Zhuang, Guang-lun, M.D</creator><creator>Liang, Xiao-yan, Ph.D</creator><creator>O, Wai-Sum, Ph.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090301</creationdate><title>In vitro–matured rat oocytes have low mitochondrial deoxyribonucleic acid and adenosine triphosphate contents and have abnormal mitochondrial redistribution</title><author>Zeng, Hai-tao, M.D., Ph.D ; Yeung, William S.B., Ph.D ; Cheung, May P.L., B.Sc ; Ho, Pak-Chung, M.D ; Lee, Calvin K.F., Ph.D ; Zhuang, Guang-lun, M.D ; Liang, Xiao-yan, Ph.D ; O, Wai-Sum, Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-622f342c365f3db2e5a905e537732501e53bd9c409df7ecd09cc6deb4a40cddc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastula - metabolism</topic><topic>Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology</topic><topic>Cells, Cultured</topic><topic>development</topic><topic>DNA, Mitochondrial - metabolism</topic><topic>Embryo Culture Techniques</topic><topic>Embryonic Development</topic><topic>Energy Metabolism</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>in vitro maturation</topic><topic>Internal Medicine</topic><topic>Medical sciences</topic><topic>Mitochondria</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Obstetrics and Gynecology</topic><topic>oocyte</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Uncoupling Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Hai-tao, M.D., Ph.D</creatorcontrib><creatorcontrib>Yeung, William S.B., Ph.D</creatorcontrib><creatorcontrib>Cheung, May P.L., B.Sc</creatorcontrib><creatorcontrib>Ho, Pak-Chung, M.D</creatorcontrib><creatorcontrib>Lee, Calvin K.F., Ph.D</creatorcontrib><creatorcontrib>Zhuang, Guang-lun, M.D</creatorcontrib><creatorcontrib>Liang, Xiao-yan, Ph.D</creatorcontrib><creatorcontrib>O, Wai-Sum, Ph.D</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Hai-tao, M.D., Ph.D</au><au>Yeung, William S.B., Ph.D</au><au>Cheung, May P.L., B.Sc</au><au>Ho, Pak-Chung, M.D</au><au>Lee, Calvin K.F., Ph.D</au><au>Zhuang, Guang-lun, M.D</au><au>Liang, Xiao-yan, Ph.D</au><au>O, Wai-Sum, Ph.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro–matured rat oocytes have low mitochondrial deoxyribonucleic acid and adenosine triphosphate contents and have abnormal mitochondrial redistribution</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>91</volume><issue>3</issue><spage>900</spage><epage>907</epage><pages>900-907</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Objective To study the development and function of mitochondria in in vitro–matured rat oocytes derived from follicles of different sizes. Design Experimental animal study. Setting Department of Anatomy at the University of Hong Kong. Animal(s) Immature female Sprague-Dawley rats that were 25 days of age. Intervention(s) Immature oocytes were collected from rat ovarian follicles of different sizes and were induced to mature in vitro. Main Outcome Measure(s) The number of copies of mitochondrial DNA, mitochondrial activity, adenosine triphosphate content of matured oocytes, and rates of fertilization and blastulation were determined. Result(s) The mitochondrial DNA copy number of oocytes increased linearly with the diameter of antral follicles. The mitochondrial DNA copy number, adenosine triphosphate content, and proportion of oocytes with peripheral distribution of mitochondria in in vitro–matured oocytes from small antral follicles were significantly lower than those from preovulatory follicles and in vivo–matured oocytes. Compared with in vitro–matured oocytes from small antral follicles, those from preovulatory follicles and in vivo–matured oocytes also had significantly better fertilization potential and higher blastulation rate. Conclusion(s) The inferior developmental potential of in vitro–matured oocytes may be attributed partly to a reduced number of mitochondria, resulting in insufficient production of adenosine triphosphate for required developmental events.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>18321496</pmid><doi>10.1016/j.fertnstert.2007.12.008</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Triphosphate - metabolism Animals Biological and medical sciences Blastula - metabolism Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology Cells, Cultured development DNA, Mitochondrial - metabolism Embryo Culture Techniques Embryonic Development Energy Metabolism Female Fertilization in Vitro Gynecology. Andrology. Obstetrics in vitro maturation Internal Medicine Medical sciences Mitochondria Mitochondria - drug effects Mitochondria - metabolism Obstetrics and Gynecology oocyte Oocytes - drug effects Oocytes - metabolism Rats Rats, Sprague-Dawley Uncoupling Agents - pharmacology |
| title | In vitro–matured rat oocytes have low mitochondrial deoxyribonucleic acid and adenosine triphosphate contents and have abnormal mitochondrial redistribution |
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