Isolation and antioxidant activity of peptides from Chinese hairy tofu

Hairy tofu is a famous Chinese snack that is made from soybeans and rich in various nutrients. In order to further explore the antioxidant peptides of hairy tofu hydrolysates, seven proteases were used to hydrolyze hairy tofu. The results of in vitro radical scavenging activity showed that hairy tof...

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Bibliographic Details
Published in:Journal of peptide science 2024-07, Vol.30 (7), p.e3572-n/a
Main Authors: Wu, Li‐Ping, Wu, Yong‐Xiang, Ke, Xiang‐Tao, Wang, Pan, Zhang, Shuo, Zhu, Yu‐Ting, Lu, Ying, Shu, Yu‐Jie, Jiang, Shang‐Yue, Li, Chang‐Jiang, Hu, Xiao‐Qian
Format: Article
Language:English
Subjects:
293T cells
amino acid sequence
antioxidant peptide
Antioxidants
Biocompatibility
Chromatography
Cytotoxicity
enzymatic hydrolysis
Food industry
Gel filtration
hairy tofu
High performance liquid chromatography
Hydrogen peroxide
Hydrolysates
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Nutrients
Pancreatin
Peptides
Scavenging
Scientific imaging
Soy products
Soybeans
Tofu
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Summary:Hairy tofu is a famous Chinese snack that is made from soybeans and rich in various nutrients. In order to further explore the antioxidant peptides of hairy tofu hydrolysates, seven proteases were used to hydrolyze hairy tofu. The results of in vitro radical scavenging activity showed that hairy tofu hydrolysates obtained by pancreatin exhibited the highest antioxidant activity. After Sephadex G‐25 gel filtration and reversed‐phase high‐performance liquid chromatography (RP‐HPLC), 97 peptides were identified in the most antioxidant fraction using liquid chromatography‐mass spectrometry/mass spectrometry (LC–MS/MS). Among them, nine peptides were synthesized and their antioxidant activities were assessed using a H2O2‐induced oxidative 293T cell model. Finally, four peptides (QCESHK, LAWNEGR, NLQGENEWDQK, and FTEMWR) at concentrations of < 50 μg/ml significantly decreased the malondialdehyde content compared with the model group, displaying in vivo antioxidant activity and low cytotoxicity. Overall, this research provided the choice of using hairy tofu peptides as antioxidant products in the pharmaceutical and food industries. To explore its antioxidant peptides, seven proteases were used to hydrolyze hairy tofu. The hairy tofu hydrolysates obtained by pancreatin exhibited the highest antioxidant activity. Four peptides (QCESHK, LAWNEGR, NLQGENEWDQK, and FTEMWR) were found to display antioxidant activity and low cytotoxicity.
ISSN:1075-2617
1099-1387
DOI:10.1002/psc.3572