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Insecticidal activity of the spider neurotoxin PPTX-04 through modulating insect voltage-gated sodium channel
Ion channels on cell membrane are molecular targets of more than half peptide neurotoxins from spiders. From Pardosa pseudoannulata, a predatory spider on a range of insect pests, we characterized a peptide neurotoxin PPTX-04 with an insecticidal activity. PPTX-04 showed high toxicity to Nilaparvata...
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| Published in: | Pesticide biochemistry and physiology 2024-05, Vol.201, p.105853-105853, Article 105853 |
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| container_start_page | 105853 |
| container_title | Pesticide biochemistry and physiology |
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| creator | Wang, Kan Yan, Yangyang Huang, Lixin Sun, Huahua Yu, Na Liu, Zewen |
| description | Ion channels on cell membrane are molecular targets of more than half peptide neurotoxins from spiders. From Pardosa pseudoannulata, a predatory spider on a range of insect pests, we characterized a peptide neurotoxin PPTX-04 with an insecticidal activity. PPTX-04 showed high toxicity to Nilaparvata lugens, a main prey of P. pseudoannulata, and the toxicity was not affected by the resistance to etofenprox (IUPAC chemical name:1-ethoxy-4-[2-methyl-1-[(3-phenoxyphenyl)methoxy]propan-2-yl]benzene, purity: 99%). On N. lugens voltage-gated sodium channel NlNav1 expressed in Xenopus oocytes, PPTX-04 prolonged the channel opening and induced tail currents, which is similar to pyrethroid insecticides. However, PPTX-04 potency on NlNav1 was not affected by mutations conferring pyrethroid resistance in insects, which revealed that PPTX-04 and pyrethroids should act on different receptors in NlNav1. In contrast, two mutations at the extracellular site 4 significantly reduced PPTX-04 potency, which indicated that PPTX-04 would act on a potential receptor containing the site 4 in NlNav1. The result from the molecular docking supported the conclusion that the binding pocket of PPTX-04 in NlNav1 should contain the site 4. In summary, PPTX-04 had high insecticidal activity through acting on a distinct receptor site in insect Nav, and was a potential resource to control insect pests and manage resistance to pyrethroids.
[Display omitted]
•Spider neurotoxin PPTX-04 showed insecticidal activity.•PPTX-04 acted on the extracellular receptor sites of insect Nav.•The receptor site is different from that of pyrethroids. |
| doi_str_mv | 10.1016/j.pestbp.2024.105853 |
| format | article |
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[Display omitted]
•Spider neurotoxin PPTX-04 showed insecticidal activity.•PPTX-04 acted on the extracellular receptor sites of insect Nav.•The receptor site is different from that of pyrethroids.</description><identifier>ISSN: 0048-3575</identifier><identifier>EISSN: 1095-9939</identifier><identifier>DOI: 10.1016/j.pestbp.2024.105853</identifier><identifier>PMID: 38685212</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Hemiptera - drug effects ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insecticides - chemistry ; Insecticides - pharmacology ; Neurotoxins - pharmacology ; Neurotoxins - toxicity ; Oocytes - drug effects ; PPTX-04 ; Pyrethrins - pharmacology ; Pyrethroid ; Resistance ; Spider neurotoxin ; Spider Venoms - chemistry ; Spider Venoms - genetics ; Spider Venoms - pharmacology ; Spiders ; Voltage-gated sodium channel Nav ; Voltage-Gated Sodium Channels - genetics ; Voltage-Gated Sodium Channels - metabolism ; Xenopus laevis</subject><ispartof>Pesticide biochemistry and physiology, 2024-05, Vol.201, p.105853-105853, Article 105853</ispartof><rights>2024 Elsevier Inc.</rights><rights>Copyright © 2024 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c311t-43c729afaf1673a1226f700accd2dccef20fc77c18271154efed19938c17fafa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38685212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Kan</creatorcontrib><creatorcontrib>Yan, Yangyang</creatorcontrib><creatorcontrib>Huang, Lixin</creatorcontrib><creatorcontrib>Sun, Huahua</creatorcontrib><creatorcontrib>Yu, Na</creatorcontrib><creatorcontrib>Liu, Zewen</creatorcontrib><title>Insecticidal activity of the spider neurotoxin PPTX-04 through modulating insect voltage-gated sodium channel</title><title>Pesticide biochemistry and physiology</title><addtitle>Pestic Biochem Physiol</addtitle><description>Ion channels on cell membrane are molecular targets of more than half peptide neurotoxins from spiders. From Pardosa pseudoannulata, a predatory spider on a range of insect pests, we characterized a peptide neurotoxin PPTX-04 with an insecticidal activity. PPTX-04 showed high toxicity to Nilaparvata lugens, a main prey of P. pseudoannulata, and the toxicity was not affected by the resistance to etofenprox (IUPAC chemical name:1-ethoxy-4-[2-methyl-1-[(3-phenoxyphenyl)methoxy]propan-2-yl]benzene, purity: 99%). On N. lugens voltage-gated sodium channel NlNav1 expressed in Xenopus oocytes, PPTX-04 prolonged the channel opening and induced tail currents, which is similar to pyrethroid insecticides. However, PPTX-04 potency on NlNav1 was not affected by mutations conferring pyrethroid resistance in insects, which revealed that PPTX-04 and pyrethroids should act on different receptors in NlNav1. In contrast, two mutations at the extracellular site 4 significantly reduced PPTX-04 potency, which indicated that PPTX-04 would act on a potential receptor containing the site 4 in NlNav1. The result from the molecular docking supported the conclusion that the binding pocket of PPTX-04 in NlNav1 should contain the site 4. In summary, PPTX-04 had high insecticidal activity through acting on a distinct receptor site in insect Nav, and was a potential resource to control insect pests and manage resistance to pyrethroids.
[Display omitted]
•Spider neurotoxin PPTX-04 showed insecticidal activity.•PPTX-04 acted on the extracellular receptor sites of insect Nav.•The receptor site is different from that of pyrethroids.</description><subject>Animals</subject><subject>Hemiptera - drug effects</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Insecticides - chemistry</subject><subject>Insecticides - pharmacology</subject><subject>Neurotoxins - pharmacology</subject><subject>Neurotoxins - toxicity</subject><subject>Oocytes - drug effects</subject><subject>PPTX-04</subject><subject>Pyrethrins - pharmacology</subject><subject>Pyrethroid</subject><subject>Resistance</subject><subject>Spider neurotoxin</subject><subject>Spider Venoms - chemistry</subject><subject>Spider Venoms - genetics</subject><subject>Spider Venoms - pharmacology</subject><subject>Spiders</subject><subject>Voltage-gated sodium channel Nav</subject><subject>Voltage-Gated Sodium Channels - genetics</subject><subject>Voltage-Gated Sodium Channels - metabolism</subject><subject>Xenopus laevis</subject><issn>0048-3575</issn><issn>1095-9939</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE9rGzEQxUVoiN2036AUHXtZV392rdWlUEyaGAzJIYHchCKNbJnd1VbSmuTbV8mmPfY0w8x785gfQl8oWVFC19-PqxFSfhpXjLC6jJq24WdoSYlsKim5_ICWhNRtxRvRLNDHlI6EEFkTeYEWvF23DaNsifrtkMBkb7zVHdalO_n8goPD-QA4jd5CxANMMeTw7Ad8d3f_WJG6bGOY9gfcBzt1Ovthj_3bJXwKXdZ7qPY6g8UpWD_12Bz0MED3CZ073SX4_F4v0cOvq_vNTbW7vd5ufu4qwynNVc2NYFI77ehacE0ZWztBiDbGMmsMOEacEcLQlglKmxocWFp-bg0VxaT5Jfo23x1j-D0VTKr3yUDX6QHClBQntRRUtqwp0nqWmhhSiuDUGH2v44uiRL2CVkc1g1avoNUMuti-vidMTz3Yf6a_ZIvgxyyA8ufJQ1TJeBgMWB8LJmWD_3_CH8zYkuQ</recordid><startdate>202405</startdate><enddate>202405</enddate><creator>Wang, Kan</creator><creator>Yan, Yangyang</creator><creator>Huang, Lixin</creator><creator>Sun, Huahua</creator><creator>Yu, Na</creator><creator>Liu, Zewen</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202405</creationdate><title>Insecticidal activity of the spider neurotoxin PPTX-04 through modulating insect voltage-gated sodium channel</title><author>Wang, Kan ; Yan, Yangyang ; Huang, Lixin ; Sun, Huahua ; Yu, Na ; Liu, Zewen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-43c729afaf1673a1226f700accd2dccef20fc77c18271154efed19938c17fafa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Hemiptera - drug effects</topic><topic>Insect Proteins - chemistry</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Insecticides - chemistry</topic><topic>Insecticides - pharmacology</topic><topic>Neurotoxins - pharmacology</topic><topic>Neurotoxins - toxicity</topic><topic>Oocytes - drug effects</topic><topic>PPTX-04</topic><topic>Pyrethrins - pharmacology</topic><topic>Pyrethroid</topic><topic>Resistance</topic><topic>Spider neurotoxin</topic><topic>Spider Venoms - chemistry</topic><topic>Spider Venoms - genetics</topic><topic>Spider Venoms - pharmacology</topic><topic>Spiders</topic><topic>Voltage-gated sodium channel Nav</topic><topic>Voltage-Gated Sodium Channels - genetics</topic><topic>Voltage-Gated Sodium Channels - metabolism</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Kan</creatorcontrib><creatorcontrib>Yan, Yangyang</creatorcontrib><creatorcontrib>Huang, Lixin</creatorcontrib><creatorcontrib>Sun, Huahua</creatorcontrib><creatorcontrib>Yu, Na</creatorcontrib><creatorcontrib>Liu, Zewen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pesticide biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Kan</au><au>Yan, Yangyang</au><au>Huang, Lixin</au><au>Sun, Huahua</au><au>Yu, Na</au><au>Liu, Zewen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insecticidal activity of the spider neurotoxin PPTX-04 through modulating insect voltage-gated sodium channel</atitle><jtitle>Pesticide biochemistry and physiology</jtitle><addtitle>Pestic Biochem Physiol</addtitle><date>2024-05</date><risdate>2024</risdate><volume>201</volume><spage>105853</spage><epage>105853</epage><pages>105853-105853</pages><artnum>105853</artnum><issn>0048-3575</issn><eissn>1095-9939</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Ion channels on cell membrane are molecular targets of more than half peptide neurotoxins from spiders. From Pardosa pseudoannulata, a predatory spider on a range of insect pests, we characterized a peptide neurotoxin PPTX-04 with an insecticidal activity. PPTX-04 showed high toxicity to Nilaparvata lugens, a main prey of P. pseudoannulata, and the toxicity was not affected by the resistance to etofenprox (IUPAC chemical name:1-ethoxy-4-[2-methyl-1-[(3-phenoxyphenyl)methoxy]propan-2-yl]benzene, purity: 99%). On N. lugens voltage-gated sodium channel NlNav1 expressed in Xenopus oocytes, PPTX-04 prolonged the channel opening and induced tail currents, which is similar to pyrethroid insecticides. However, PPTX-04 potency on NlNav1 was not affected by mutations conferring pyrethroid resistance in insects, which revealed that PPTX-04 and pyrethroids should act on different receptors in NlNav1. In contrast, two mutations at the extracellular site 4 significantly reduced PPTX-04 potency, which indicated that PPTX-04 would act on a potential receptor containing the site 4 in NlNav1. The result from the molecular docking supported the conclusion that the binding pocket of PPTX-04 in NlNav1 should contain the site 4. In summary, PPTX-04 had high insecticidal activity through acting on a distinct receptor site in insect Nav, and was a potential resource to control insect pests and manage resistance to pyrethroids.
[Display omitted]
•Spider neurotoxin PPTX-04 showed insecticidal activity.•PPTX-04 acted on the extracellular receptor sites of insect Nav.•The receptor site is different from that of pyrethroids.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38685212</pmid><doi>10.1016/j.pestbp.2024.105853</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Hemiptera - drug effects Insect Proteins - chemistry Insect Proteins - genetics Insect Proteins - metabolism Insecticides - chemistry Insecticides - pharmacology Neurotoxins - pharmacology Neurotoxins - toxicity Oocytes - drug effects PPTX-04 Pyrethrins - pharmacology Pyrethroid Resistance Spider neurotoxin Spider Venoms - chemistry Spider Venoms - genetics Spider Venoms - pharmacology Spiders Voltage-gated sodium channel Nav Voltage-Gated Sodium Channels - genetics Voltage-Gated Sodium Channels - metabolism Xenopus laevis |
| title | Insecticidal activity of the spider neurotoxin PPTX-04 through modulating insect voltage-gated sodium channel |
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