Insecticidal activity of the spider neurotoxin PPTX-04 through modulating insect voltage-gated sodium channel

Ion channels on cell membrane are molecular targets of more than half peptide neurotoxins from spiders. From Pardosa pseudoannulata, a predatory spider on a range of insect pests, we characterized a peptide neurotoxin PPTX-04 with an insecticidal activity. PPTX-04 showed high toxicity to Nilaparvata...

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Bibliographic Details
Published in:Pesticide biochemistry and physiology 2024-05, Vol.201, p.105853-105853, Article 105853
Main Authors: Wang, Kan, Yan, Yangyang, Huang, Lixin, Sun, Huahua, Yu, Na, Liu, Zewen
Format: Article
Language:English
Subjects:
Animals
Hemiptera - drug effects
Insect Proteins - chemistry
Insect Proteins - genetics
Insect Proteins - metabolism
Insecticides - chemistry
Insecticides - pharmacology
Neurotoxins - pharmacology
Neurotoxins - toxicity
Oocytes - drug effects
PPTX-04
Pyrethrins - pharmacology
Pyrethroid
Resistance
Spider neurotoxin
Spider Venoms - chemistry
Spider Venoms - genetics
Spider Venoms - pharmacology
Spiders
Voltage-gated sodium channel Nav
Voltage-Gated Sodium Channels - genetics
Voltage-Gated Sodium Channels - metabolism
Xenopus laevis
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Summary:Ion channels on cell membrane are molecular targets of more than half peptide neurotoxins from spiders. From Pardosa pseudoannulata, a predatory spider on a range of insect pests, we characterized a peptide neurotoxin PPTX-04 with an insecticidal activity. PPTX-04 showed high toxicity to Nilaparvata lugens, a main prey of P. pseudoannulata, and the toxicity was not affected by the resistance to etofenprox (IUPAC chemical name:1-ethoxy-4-[2-methyl-1-[(3-phenoxyphenyl)methoxy]propan-2-yl]benzene, purity: 99%). On N. lugens voltage-gated sodium channel NlNav1 expressed in Xenopus oocytes, PPTX-04 prolonged the channel opening and induced tail currents, which is similar to pyrethroid insecticides. However, PPTX-04 potency on NlNav1 was not affected by mutations conferring pyrethroid resistance in insects, which revealed that PPTX-04 and pyrethroids should act on different receptors in NlNav1. In contrast, two mutations at the extracellular site 4 significantly reduced PPTX-04 potency, which indicated that PPTX-04 would act on a potential receptor containing the site 4 in NlNav1. The result from the molecular docking supported the conclusion that the binding pocket of PPTX-04 in NlNav1 should contain the site 4. In summary, PPTX-04 had high insecticidal activity through acting on a distinct receptor site in insect Nav, and was a potential resource to control insect pests and manage resistance to pyrethroids. [Display omitted] •Spider neurotoxin PPTX-04 showed insecticidal activity.•PPTX-04 acted on the extracellular receptor sites of insect Nav.•The receptor site is different from that of pyrethroids.
ISSN:0048-3575
1095-9939
DOI:10.1016/j.pestbp.2024.105853