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Recombinant human collagen I/carboxymethyl chitosan hydrogel loaded with long-term released hUCMSCs derived exosomes promotes skin wound repair
Stem cell exosomes are beneficial in accelerating wound repair. However, the therapeutic function is limited due to its rapid clearance in vivo. To improve the functionality of exosomes in cutaneous wound healing, a novel hydrogel was designed and fabricated by recombinant human collagen I and carbo...
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| Published in: | International journal of biological macromolecules 2024-04, Vol.265 (Pt 1), p.130843-130843, Article 130843 |
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| container_end_page | 130843 |
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| container_title | International journal of biological macromolecules |
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| creator | Wu, Qiong Guo, Yayuan Li, Hongwei Zhang, Dan Wang, Shixu Hou, Jianing Cheng, Nanqiong Huang, Mengfei Luo, Linna Li, Yuan Zhao, Yurong Tan, Hong Jin, Changxin |
| description | Stem cell exosomes are beneficial in accelerating wound repair. However, the therapeutic function is limited due to its rapid clearance in vivo. To improve the functionality of exosomes in cutaneous wound healing, a novel hydrogel was designed and fabricated by recombinant human collagen I and carboxymethyl chitosan loaded with exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs), named as the rhCol I/CMC-Exos hydrogel.
Exosomes were extracted from hUCMSCs and were characterizated by TEM (Transmission Electron Microscopy), and biomarker detection. The rhCol I hydrogel, rhCol I/carboxymethyl chitosan (rhCol I/CMC) hydrogel and the rhCol I/CMC-Exos hydrogel composites were cross-linked by genipin. These materials were assessed and compared for their physical characteristics, including cross-sectional morphology, porosity, pore distribution, and hydrophilicity. Cell biocompatibility on biomaterials was investigated using scanning electron microscopy and CFDA staining, as well as assessed in vivo through histological examination of major organs in mice. Effects of the hydrogel composite on wound healing were further evaluated by using the full-thickness skin defect mice model.
Successful extraction of hUCMSCs-derived exosomes was confirmed by TEM，Western Blotting and flow cytometry. The synthesized rhCol I/CMC-Exos hydrogel composite exhibited cytocompatibility and promoted cell growth in vitro. The rhCol I/CMC-Exos hydrogel showed sustained release of exosomes. In the mice full skin-defects model, the rhCol I/CMC-Exos-treated group showed superior wound healing efficiency, with 15 % faster wound closure compared to controls. Histological examinations revealed thicker dermis formation and more balanced collagen deposition in wounds treated with rhCol I/CMC-Exos hydrogel. Mechanistically, the application of rhCol I/CMC-Exos hydrogel increased fibroblasts proliferation, alleviated inflammation responses as well as promoted angiogenesis, thereby was beneficial in promoting skin wound healing and regeneration.
Our study, for the first time, introduced recombinant human Collagen I in fabricating a novel hydrogel loaded with hUCMSCs-derived exosomes, which effectively promoted skin wound closure and regeneration, demonstrating a great potential in severe skin wound healing treatment. |
| doi_str_mv | 10.1016/j.ijbiomac.2024.130843 |
| format | article |
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Exosomes were extracted from hUCMSCs and were characterizated by TEM (Transmission Electron Microscopy), and biomarker detection. The rhCol I hydrogel, rhCol I/carboxymethyl chitosan (rhCol I/CMC) hydrogel and the rhCol I/CMC-Exos hydrogel composites were cross-linked by genipin. These materials were assessed and compared for their physical characteristics, including cross-sectional morphology, porosity, pore distribution, and hydrophilicity. Cell biocompatibility on biomaterials was investigated using scanning electron microscopy and CFDA staining, as well as assessed in vivo through histological examination of major organs in mice. Effects of the hydrogel composite on wound healing were further evaluated by using the full-thickness skin defect mice model.
Successful extraction of hUCMSCs-derived exosomes was confirmed by TEM，Western Blotting and flow cytometry. The synthesized rhCol I/CMC-Exos hydrogel composite exhibited cytocompatibility and promoted cell growth in vitro. The rhCol I/CMC-Exos hydrogel showed sustained release of exosomes. In the mice full skin-defects model, the rhCol I/CMC-Exos-treated group showed superior wound healing efficiency, with 15 % faster wound closure compared to controls. Histological examinations revealed thicker dermis formation and more balanced collagen deposition in wounds treated with rhCol I/CMC-Exos hydrogel. Mechanistically, the application of rhCol I/CMC-Exos hydrogel increased fibroblasts proliferation, alleviated inflammation responses as well as promoted angiogenesis, thereby was beneficial in promoting skin wound healing and regeneration.
Our study, for the first time, introduced recombinant human Collagen I in fabricating a novel hydrogel loaded with hUCMSCs-derived exosomes, which effectively promoted skin wound closure and regeneration, demonstrating a great potential in severe skin wound healing treatment.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2024.130843</identifier><identifier>PMID: 38484819</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Carboxymethyl chitosan ; Exosomes ; Hydrogel ; Recombinant human collagen I ; Wound healing</subject><ispartof>International journal of biological macromolecules, 2024-04, Vol.265 (Pt 1), p.130843-130843, Article 130843</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c315t-8d342bebc7fc930fb7406b60337aa17898fc9935d202633e86ea5f1508102e2f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38484819$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Qiong</creatorcontrib><creatorcontrib>Guo, Yayuan</creatorcontrib><creatorcontrib>Li, Hongwei</creatorcontrib><creatorcontrib>Zhang, Dan</creatorcontrib><creatorcontrib>Wang, Shixu</creatorcontrib><creatorcontrib>Hou, Jianing</creatorcontrib><creatorcontrib>Cheng, Nanqiong</creatorcontrib><creatorcontrib>Huang, Mengfei</creatorcontrib><creatorcontrib>Luo, Linna</creatorcontrib><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Zhao, Yurong</creatorcontrib><creatorcontrib>Tan, Hong</creatorcontrib><creatorcontrib>Jin, Changxin</creatorcontrib><title>Recombinant human collagen I/carboxymethyl chitosan hydrogel loaded with long-term released hUCMSCs derived exosomes promotes skin wound repair</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Stem cell exosomes are beneficial in accelerating wound repair. However, the therapeutic function is limited due to its rapid clearance in vivo. To improve the functionality of exosomes in cutaneous wound healing, a novel hydrogel was designed and fabricated by recombinant human collagen I and carboxymethyl chitosan loaded with exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs), named as the rhCol I/CMC-Exos hydrogel.
Exosomes were extracted from hUCMSCs and were characterizated by TEM (Transmission Electron Microscopy), and biomarker detection. The rhCol I hydrogel, rhCol I/carboxymethyl chitosan (rhCol I/CMC) hydrogel and the rhCol I/CMC-Exos hydrogel composites were cross-linked by genipin. These materials were assessed and compared for their physical characteristics, including cross-sectional morphology, porosity, pore distribution, and hydrophilicity. Cell biocompatibility on biomaterials was investigated using scanning electron microscopy and CFDA staining, as well as assessed in vivo through histological examination of major organs in mice. Effects of the hydrogel composite on wound healing were further evaluated by using the full-thickness skin defect mice model.
Successful extraction of hUCMSCs-derived exosomes was confirmed by TEM，Western Blotting and flow cytometry. The synthesized rhCol I/CMC-Exos hydrogel composite exhibited cytocompatibility and promoted cell growth in vitro. The rhCol I/CMC-Exos hydrogel showed sustained release of exosomes. In the mice full skin-defects model, the rhCol I/CMC-Exos-treated group showed superior wound healing efficiency, with 15 % faster wound closure compared to controls. Histological examinations revealed thicker dermis formation and more balanced collagen deposition in wounds treated with rhCol I/CMC-Exos hydrogel. Mechanistically, the application of rhCol I/CMC-Exos hydrogel increased fibroblasts proliferation, alleviated inflammation responses as well as promoted angiogenesis, thereby was beneficial in promoting skin wound healing and regeneration.
Our study, for the first time, introduced recombinant human Collagen I in fabricating a novel hydrogel loaded with hUCMSCs-derived exosomes, which effectively promoted skin wound closure and regeneration, demonstrating a great potential in severe skin wound healing treatment.</description><subject>Carboxymethyl chitosan</subject><subject>Exosomes</subject><subject>Hydrogel</subject><subject>Recombinant human collagen I</subject><subject>Wound healing</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFUV1PHCEUJU1NXbf9C4bHvswKw3wwbzab2ppoTGx9Jgzc2WEdYAuMur_Cvyxmta-Gh3s5nMP9OAidUrKihDZn25XZ9sZbqVYlKasVZYRX7BNaUN52BSGEfUYLQita8Px0jE5i3Ga0qSn_go4Zr_Kh3QI934LytjdOuoTH2UqHlZ8muQGHL8-UDL1_2ltI437CajTJx8wY9zr4DUx48lKDxo8mjTl3myJBsDjABDJmfLxbX_9ZR6whmId8hycfvYWId8Fbn3IS743Dj352Oqt20oSv6GiQU4Rvb3GJ7i5-_l3_Lq5ufl2uf1wVitE6FVyzquyhV-2gOkaGvq1I0zeEsVZK2vKOZ7xjtc7LaRgD3oCsB1oTTkkJ5cCW6Pvh39zKvxliEtZEBXlyB36OouxqXnaMZfUSNQeqCj7GAIPYBWNl2AtKxKsZYivezRCvZoiDGVl4-lZj7i3o_7L37WfC-YEAedIHA0FEZcAp0CaASkJ781GNF9wYoQQ</recordid><startdate>202404</startdate><enddate>202404</enddate><creator>Wu, Qiong</creator><creator>Guo, Yayuan</creator><creator>Li, Hongwei</creator><creator>Zhang, Dan</creator><creator>Wang, Shixu</creator><creator>Hou, Jianing</creator><creator>Cheng, Nanqiong</creator><creator>Huang, Mengfei</creator><creator>Luo, Linna</creator><creator>Li, Yuan</creator><creator>Zhao, Yurong</creator><creator>Tan, Hong</creator><creator>Jin, Changxin</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202404</creationdate><title>Recombinant human collagen I/carboxymethyl chitosan hydrogel loaded with long-term released hUCMSCs derived exosomes promotes skin wound repair</title><author>Wu, Qiong ; Guo, Yayuan ; Li, Hongwei ; Zhang, Dan ; Wang, Shixu ; Hou, Jianing ; Cheng, Nanqiong ; Huang, Mengfei ; Luo, Linna ; Li, Yuan ; Zhao, Yurong ; Tan, Hong ; Jin, Changxin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-8d342bebc7fc930fb7406b60337aa17898fc9935d202633e86ea5f1508102e2f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Carboxymethyl chitosan</topic><topic>Exosomes</topic><topic>Hydrogel</topic><topic>Recombinant human collagen I</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Qiong</creatorcontrib><creatorcontrib>Guo, Yayuan</creatorcontrib><creatorcontrib>Li, Hongwei</creatorcontrib><creatorcontrib>Zhang, Dan</creatorcontrib><creatorcontrib>Wang, Shixu</creatorcontrib><creatorcontrib>Hou, Jianing</creatorcontrib><creatorcontrib>Cheng, Nanqiong</creatorcontrib><creatorcontrib>Huang, Mengfei</creatorcontrib><creatorcontrib>Luo, Linna</creatorcontrib><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Zhao, Yurong</creatorcontrib><creatorcontrib>Tan, Hong</creatorcontrib><creatorcontrib>Jin, Changxin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Qiong</au><au>Guo, Yayuan</au><au>Li, Hongwei</au><au>Zhang, Dan</au><au>Wang, Shixu</au><au>Hou, Jianing</au><au>Cheng, Nanqiong</au><au>Huang, Mengfei</au><au>Luo, Linna</au><au>Li, Yuan</au><au>Zhao, Yurong</au><au>Tan, Hong</au><au>Jin, Changxin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant human collagen I/carboxymethyl chitosan hydrogel loaded with long-term released hUCMSCs derived exosomes promotes skin wound repair</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2024-04</date><risdate>2024</risdate><volume>265</volume><issue>Pt 1</issue><spage>130843</spage><epage>130843</epage><pages>130843-130843</pages><artnum>130843</artnum><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Stem cell exosomes are beneficial in accelerating wound repair. However, the therapeutic function is limited due to its rapid clearance in vivo. To improve the functionality of exosomes in cutaneous wound healing, a novel hydrogel was designed and fabricated by recombinant human collagen I and carboxymethyl chitosan loaded with exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs), named as the rhCol I/CMC-Exos hydrogel.
Exosomes were extracted from hUCMSCs and were characterizated by TEM (Transmission Electron Microscopy), and biomarker detection. The rhCol I hydrogel, rhCol I/carboxymethyl chitosan (rhCol I/CMC) hydrogel and the rhCol I/CMC-Exos hydrogel composites were cross-linked by genipin. These materials were assessed and compared for their physical characteristics, including cross-sectional morphology, porosity, pore distribution, and hydrophilicity. Cell biocompatibility on biomaterials was investigated using scanning electron microscopy and CFDA staining, as well as assessed in vivo through histological examination of major organs in mice. Effects of the hydrogel composite on wound healing were further evaluated by using the full-thickness skin defect mice model.
Successful extraction of hUCMSCs-derived exosomes was confirmed by TEM，Western Blotting and flow cytometry. The synthesized rhCol I/CMC-Exos hydrogel composite exhibited cytocompatibility and promoted cell growth in vitro. The rhCol I/CMC-Exos hydrogel showed sustained release of exosomes. In the mice full skin-defects model, the rhCol I/CMC-Exos-treated group showed superior wound healing efficiency, with 15 % faster wound closure compared to controls. Histological examinations revealed thicker dermis formation and more balanced collagen deposition in wounds treated with rhCol I/CMC-Exos hydrogel. Mechanistically, the application of rhCol I/CMC-Exos hydrogel increased fibroblasts proliferation, alleviated inflammation responses as well as promoted angiogenesis, thereby was beneficial in promoting skin wound healing and regeneration.
Our study, for the first time, introduced recombinant human Collagen I in fabricating a novel hydrogel loaded with hUCMSCs-derived exosomes, which effectively promoted skin wound closure and regeneration, demonstrating a great potential in severe skin wound healing treatment.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38484819</pmid><doi>10.1016/j.ijbiomac.2024.130843</doi><tpages>1</tpages></addata></record> |
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| source | ScienceDirect Freedom Collection |
| subjects | Carboxymethyl chitosan Exosomes Hydrogel Recombinant human collagen I Wound healing |
| title | Recombinant human collagen I/carboxymethyl chitosan hydrogel loaded with long-term released hUCMSCs derived exosomes promotes skin wound repair |
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