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Activity of natural occurring entomopathogenic fungi on nymphal and adult stages of Philaenus spumarius
[Display omitted] •Four entomopathogenic fungi (EPF) associated with Philaenus spumarius were found.•Lecanicillium aphanocladii shows the highest entomopathogenicity on spittlebug nymphs.•L, aphanocladii blastospores cause mortality rate similar to Beauveria bassiana (90%)•No induced mortality of EP...
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| Published in: | Journal of invertebrate pathology 2024-06, Vol.204, p.108078-108078, Article 108078 |
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| creator | Bodino, Nicola Barbera, Riccardo González-Mas, Natalia Demichelis, Stefano Bosco, Domenico Dolci, Paola |
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•Four entomopathogenic fungi (EPF) associated with Philaenus spumarius were found.•Lecanicillium aphanocladii shows the highest entomopathogenicity on spittlebug nymphs.•L, aphanocladii blastospores cause mortality rate similar to Beauveria bassiana (90%)•No induced mortality of EPF was observed on adult stage of P, spumarius.
The spittlebug Philaenus spumarius (Hemiptera: Aphrophoridae) is the predominant vector of Xylella fastidiosa (Xanthomonadales: Xanthomonadaceae) in Apulia, Italy and the rest of Europe. Current control strategies of the insect vector rely on mechanical management of nymphal stages and insecticide application against adult populations. Entomopathogenic fungi (EPF) are biological control agents naturally attacking spittlebugs and may effectively reduce population levels of host species. Different experimental trials in controlled conditions have been performed to i) identify naturally occurring EPF on P, spumarius in Northwestern Italy, and ii) evaluate the potential for biocontrol of the isolated strains on both nymphal and adult stages of the spittlebug. Four EPF species were isolated from dead P. spumarius collected in semi-field conditions: Beauveria bassiana, Conidiobolus coronatus, Fusarium equiseti and Lecanicillium aphanocladii. All the fungal isolates showed entomopathogenic potential against nymphal stages of P. spumarius (≈ 45 % mortality), except for F. equiseti, in preliminary trials. No induced mortality was observed on adult stage. Lecanicillium aphanocladii was the most promising fungus and its pathogenicity against spittlebug nymphs was further tested in different formulations (conidia vs blastospores) and with natural adjuvants. Blastospore formulation was the most effective in killing nymphal instars and reducing the emergence rate of P, spumarius adults, reaching mortality levels (90%) similar to those of the commercial product Naturalis®, while no or adverse effect of natural adjuvants was recorded. The encouraging results of this study pave way for testing EPF isolates against P, spumarius in field conditions and find new environmentally friendly control strategies against insect vectors of X. fastidiosa. |
| doi_str_mv | 10.1016/j.jip.2024.108078 |
| format | article |
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•Four entomopathogenic fungi (EPF) associated with Philaenus spumarius were found.•Lecanicillium aphanocladii shows the highest entomopathogenicity on spittlebug nymphs.•L, aphanocladii blastospores cause mortality rate similar to Beauveria bassiana (90%)•No induced mortality of EPF was observed on adult stage of P, spumarius.
The spittlebug Philaenus spumarius (Hemiptera: Aphrophoridae) is the predominant vector of Xylella fastidiosa (Xanthomonadales: Xanthomonadaceae) in Apulia, Italy and the rest of Europe. Current control strategies of the insect vector rely on mechanical management of nymphal stages and insecticide application against adult populations. Entomopathogenic fungi (EPF) are biological control agents naturally attacking spittlebugs and may effectively reduce population levels of host species. Different experimental trials in controlled conditions have been performed to i) identify naturally occurring EPF on P, spumarius in Northwestern Italy, and ii) evaluate the potential for biocontrol of the isolated strains on both nymphal and adult stages of the spittlebug. Four EPF species were isolated from dead P. spumarius collected in semi-field conditions: Beauveria bassiana, Conidiobolus coronatus, Fusarium equiseti and Lecanicillium aphanocladii. All the fungal isolates showed entomopathogenic potential against nymphal stages of P. spumarius (≈ 45 % mortality), except for F. equiseti, in preliminary trials. No induced mortality was observed on adult stage. Lecanicillium aphanocladii was the most promising fungus and its pathogenicity against spittlebug nymphs was further tested in different formulations (conidia vs blastospores) and with natural adjuvants. Blastospore formulation was the most effective in killing nymphal instars and reducing the emergence rate of P, spumarius adults, reaching mortality levels (90%) similar to those of the commercial product Naturalis®, while no or adverse effect of natural adjuvants was recorded. The encouraging results of this study pave way for testing EPF isolates against P, spumarius in field conditions and find new environmentally friendly control strategies against insect vectors of X. fastidiosa.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1016/j.jip.2024.108078</identifier><identifier>PMID: 38438078</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Beauveria - pathogenicity ; Beauveria - physiology ; Biological control agent ; Blastospore ; Fusarium ; Hemiptera - microbiology ; Hypocreales - pathogenicity ; Hypocreales - physiology ; Insect pathogenic fungi ; Insect Vectors - microbiology ; Integrated pest management ; Italy ; Lecanicillium aphanocladii ; Nymph - growth & development ; Nymph - microbiology ; Pest Control, Biological - methods ; Xylella - physiology ; Xylella fastidiosa</subject><ispartof>Journal of invertebrate pathology, 2024-06, Vol.204, p.108078-108078, Article 108078</ispartof><rights>2024 The Authors</rights><rights>Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c348t-18ec3de1c7ee4f2d9378e570739685876b1d2b11f63e005d2faf8ea2b635b203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38438078$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bodino, Nicola</creatorcontrib><creatorcontrib>Barbera, Riccardo</creatorcontrib><creatorcontrib>González-Mas, Natalia</creatorcontrib><creatorcontrib>Demichelis, Stefano</creatorcontrib><creatorcontrib>Bosco, Domenico</creatorcontrib><creatorcontrib>Dolci, Paola</creatorcontrib><title>Activity of natural occurring entomopathogenic fungi on nymphal and adult stages of Philaenus spumarius</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>[Display omitted]
•Four entomopathogenic fungi (EPF) associated with Philaenus spumarius were found.•Lecanicillium aphanocladii shows the highest entomopathogenicity on spittlebug nymphs.•L, aphanocladii blastospores cause mortality rate similar to Beauveria bassiana (90%)•No induced mortality of EPF was observed on adult stage of P, spumarius.
The spittlebug Philaenus spumarius (Hemiptera: Aphrophoridae) is the predominant vector of Xylella fastidiosa (Xanthomonadales: Xanthomonadaceae) in Apulia, Italy and the rest of Europe. Current control strategies of the insect vector rely on mechanical management of nymphal stages and insecticide application against adult populations. Entomopathogenic fungi (EPF) are biological control agents naturally attacking spittlebugs and may effectively reduce population levels of host species. Different experimental trials in controlled conditions have been performed to i) identify naturally occurring EPF on P, spumarius in Northwestern Italy, and ii) evaluate the potential for biocontrol of the isolated strains on both nymphal and adult stages of the spittlebug. Four EPF species were isolated from dead P. spumarius collected in semi-field conditions: Beauveria bassiana, Conidiobolus coronatus, Fusarium equiseti and Lecanicillium aphanocladii. All the fungal isolates showed entomopathogenic potential against nymphal stages of P. spumarius (≈ 45 % mortality), except for F. equiseti, in preliminary trials. No induced mortality was observed on adult stage. Lecanicillium aphanocladii was the most promising fungus and its pathogenicity against spittlebug nymphs was further tested in different formulations (conidia vs blastospores) and with natural adjuvants. Blastospore formulation was the most effective in killing nymphal instars and reducing the emergence rate of P, spumarius adults, reaching mortality levels (90%) similar to those of the commercial product Naturalis®, while no or adverse effect of natural adjuvants was recorded. The encouraging results of this study pave way for testing EPF isolates against P, spumarius in field conditions and find new environmentally friendly control strategies against insect vectors of X. fastidiosa.</description><subject>Animals</subject><subject>Beauveria - pathogenicity</subject><subject>Beauveria - physiology</subject><subject>Biological control agent</subject><subject>Blastospore</subject><subject>Fusarium</subject><subject>Hemiptera - microbiology</subject><subject>Hypocreales - pathogenicity</subject><subject>Hypocreales - physiology</subject><subject>Insect pathogenic fungi</subject><subject>Insect Vectors - microbiology</subject><subject>Integrated pest management</subject><subject>Italy</subject><subject>Lecanicillium aphanocladii</subject><subject>Nymph - growth & development</subject><subject>Nymph - microbiology</subject><subject>Pest Control, Biological - methods</subject><subject>Xylella - physiology</subject><subject>Xylella fastidiosa</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE1L7DAUQIMoOk_9AW4kSzcd8zFtM7gSUZ8gPBfuQ5rcdjK0Sc2HMP_-ZRh16Sq5cO4hOQhdUbKkhDa32-XWzktG2KrMgrTiCC0oWTdVGepjtCCEsYoRSs_Qnxi3pNzqZn2KzrhY8T2_QMO9TvbTph32PXYq5aBG7LXOIVg3YHDJT35WaeMHcFbjPrvBYu-w203zprDKGaxMHhOOSQ0Q9563jR0VuBxxnPOkgs3xAp30aoxw-XWeo_enx_eHv9Xrv-eXh_vXSvOVSBUVoLkBqluAVc_MmrcC6pa0fN2IWrRNRw3rKO0bDoTUhvWqF6BY1_C6Y4Sfo5uDdg7-I0NMcrJRwzgqBz5HyYqwJYw3dUHpAdXBxxigl3Ow5bE7SYnc55VbWfLKfV55yFt2rr_0uZvA_Gx89yzA3QGA8sdPC0FGbcFpMDaATtJ4-4v-P5jFjFs</recordid><startdate>202406</startdate><enddate>202406</enddate><creator>Bodino, Nicola</creator><creator>Barbera, Riccardo</creator><creator>González-Mas, Natalia</creator><creator>Demichelis, Stefano</creator><creator>Bosco, Domenico</creator><creator>Dolci, Paola</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202406</creationdate><title>Activity of natural occurring entomopathogenic fungi on nymphal and adult stages of Philaenus spumarius</title><author>Bodino, Nicola ; Barbera, Riccardo ; González-Mas, Natalia ; Demichelis, Stefano ; Bosco, Domenico ; Dolci, Paola</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-18ec3de1c7ee4f2d9378e570739685876b1d2b11f63e005d2faf8ea2b635b203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Beauveria - pathogenicity</topic><topic>Beauveria - physiology</topic><topic>Biological control agent</topic><topic>Blastospore</topic><topic>Fusarium</topic><topic>Hemiptera - microbiology</topic><topic>Hypocreales - pathogenicity</topic><topic>Hypocreales - physiology</topic><topic>Insect pathogenic fungi</topic><topic>Insect Vectors - microbiology</topic><topic>Integrated pest management</topic><topic>Italy</topic><topic>Lecanicillium aphanocladii</topic><topic>Nymph - growth & development</topic><topic>Nymph - microbiology</topic><topic>Pest Control, Biological - methods</topic><topic>Xylella - physiology</topic><topic>Xylella fastidiosa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bodino, Nicola</creatorcontrib><creatorcontrib>Barbera, Riccardo</creatorcontrib><creatorcontrib>González-Mas, Natalia</creatorcontrib><creatorcontrib>Demichelis, Stefano</creatorcontrib><creatorcontrib>Bosco, Domenico</creatorcontrib><creatorcontrib>Dolci, Paola</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bodino, Nicola</au><au>Barbera, Riccardo</au><au>González-Mas, Natalia</au><au>Demichelis, Stefano</au><au>Bosco, Domenico</au><au>Dolci, Paola</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activity of natural occurring entomopathogenic fungi on nymphal and adult stages of Philaenus spumarius</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>2024-06</date><risdate>2024</risdate><volume>204</volume><spage>108078</spage><epage>108078</epage><pages>108078-108078</pages><artnum>108078</artnum><issn>0022-2011</issn><eissn>1096-0805</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>[Display omitted]
•Four entomopathogenic fungi (EPF) associated with Philaenus spumarius were found.•Lecanicillium aphanocladii shows the highest entomopathogenicity on spittlebug nymphs.•L, aphanocladii blastospores cause mortality rate similar to Beauveria bassiana (90%)•No induced mortality of EPF was observed on adult stage of P, spumarius.
The spittlebug Philaenus spumarius (Hemiptera: Aphrophoridae) is the predominant vector of Xylella fastidiosa (Xanthomonadales: Xanthomonadaceae) in Apulia, Italy and the rest of Europe. Current control strategies of the insect vector rely on mechanical management of nymphal stages and insecticide application against adult populations. Entomopathogenic fungi (EPF) are biological control agents naturally attacking spittlebugs and may effectively reduce population levels of host species. Different experimental trials in controlled conditions have been performed to i) identify naturally occurring EPF on P, spumarius in Northwestern Italy, and ii) evaluate the potential for biocontrol of the isolated strains on both nymphal and adult stages of the spittlebug. Four EPF species were isolated from dead P. spumarius collected in semi-field conditions: Beauveria bassiana, Conidiobolus coronatus, Fusarium equiseti and Lecanicillium aphanocladii. All the fungal isolates showed entomopathogenic potential against nymphal stages of P. spumarius (≈ 45 % mortality), except for F. equiseti, in preliminary trials. No induced mortality was observed on adult stage. Lecanicillium aphanocladii was the most promising fungus and its pathogenicity against spittlebug nymphs was further tested in different formulations (conidia vs blastospores) and with natural adjuvants. Blastospore formulation was the most effective in killing nymphal instars and reducing the emergence rate of P, spumarius adults, reaching mortality levels (90%) similar to those of the commercial product Naturalis®, while no or adverse effect of natural adjuvants was recorded. The encouraging results of this study pave way for testing EPF isolates against P, spumarius in field conditions and find new environmentally friendly control strategies against insect vectors of X. fastidiosa.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>38438078</pmid><doi>10.1016/j.jip.2024.108078</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Beauveria - pathogenicity Beauveria - physiology Biological control agent Blastospore Fusarium Hemiptera - microbiology Hypocreales - pathogenicity Hypocreales - physiology Insect pathogenic fungi Insect Vectors - microbiology Integrated pest management Italy Lecanicillium aphanocladii Nymph - growth & development Nymph - microbiology Pest Control, Biological - methods Xylella - physiology Xylella fastidiosa |
| title | Activity of natural occurring entomopathogenic fungi on nymphal and adult stages of Philaenus spumarius |
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