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Nanoparticle-delivered RNAi-based pesticide target screening for the rice pest white-backed planthopper and risk assessment for a natural predator
Vacuolar-type (H+)-ATPase (vATPase) is a conserved multi-subunit eukaryotic enzyme composed of 14 subunits that form a functional complex consisting of an ATP-hydrolytic domain (V1) and a proton-translocation domain (V0). ATP hydrolysis and subsequent H+ translocation rely heavily on a fully assembl...
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| Published in: | The Science of the total environment 2024-05, Vol.926, p.171286-171286, Article 171286 |
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| creator | Ma, Yun-Feng Zhao, Ya-Qin Zhou, Yang-yuntao Feng, Hong-Yan Gong, Lang-Lang Zhang, Meng-Qi Hull, J. Joe Dewer, Youssef Roy, Amit Smagghe, Guy He, Ming He, Peng |
| description | Vacuolar-type (H+)-ATPase (vATPase) is a conserved multi-subunit eukaryotic enzyme composed of 14 subunits that form a functional complex consisting of an ATP-hydrolytic domain (V1) and a proton-translocation domain (V0). ATP hydrolysis and subsequent H+ translocation rely heavily on a fully assembled V1/V0 complex. Since vATPase is crucial for insect survival, it is a viable molecular target for pest control. However, detailed functional analyses of the 14 subunits and their suitability for pest control have not been fully explored in a single insect species. In this study, we identified 22 vATPase subunit transcripts that correspond to 13 subunits (A1, A2, B, C, D, E, F, G, H, a1, a2, c and d) in the white-backed planthopper (WBPH), Sogatella furcifera, a major hemipteran pest of rice. RNAi screens using microinjection and spray-based methods revealed that the SfVHA-F, SfVHA-a2 and SfVHA-c2 subunits are critical. Furthermore, star polymer (SPc) nanoparticles were utilized to conduct spray-induced and nanoparticle-delivered gene silencing (SI-NDGS) to evaluate the pest control efficacy of RNAi targeting the SfVHA-F, SfVHA-a2 and SfVHA-c2 transcripts. Target mRNA levels and vATPase enzymatic activity were both reduced. Honeydew excreta was likewise reduced in WBPH treated with dsRNAs targeting SfVHA-F, SfVHA-a2 and SfVHA-c2. To assess the environmental safety of the nanoparticle-wrapped dsRNAs, Cyrtorhinus lividipennis Reuter, a major natural enemy of planthoppers, was also sprayed with dsRNAs targeting SfVHA-F, SfVHA-a2 and SfVHA-c2. Post-spray effects of dsSfVHA-a2 and dsSfVHA-c2 on C. lividipennis were innocuous. This study identifies SfVHA-a2 and SfVHA-c2 as promising targets for biorational control of WBPH and lays the foundation for developing environment-friendly RNAi biopesticides.
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•Twenty-two vATPase subunit transcripts were identified in Sogatella furcifera.•vATPase subunit transcripts were screened by RNAi as potential control targets.•Nanoparticle-delivery was used to increase the pest control efficacy.•Assessment of potential non-target organism effects in Cyrtorhinus lividipennis |
| doi_str_mv | 10.1016/j.scitotenv.2024.171286 |
| format | article |
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[Display omitted]
•Twenty-two vATPase subunit transcripts were identified in Sogatella furcifera.•vATPase subunit transcripts were screened by RNAi as potential control targets.•Nanoparticle-delivery was used to increase the pest control efficacy.•Assessment of potential non-target organism effects in Cyrtorhinus lividipennis</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2024.171286</identifier><identifier>PMID: 38428617</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adenosine Triphosphate ; Animals ; Hemiptera ; Heteroptera ; Natural enemy ; Oryza - genetics ; Pesticides ; Risk Assessment ; RNA Interference ; Spray-induced and nanoparticle-delivered gene silencing ; Star polymer ; vATPase ; White-backed planthopper</subject><ispartof>The Science of the total environment, 2024-05, Vol.926, p.171286-171286, Article 171286</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-861084f9fd18ea61f10e331d69548e9d0a0be30d575e102ec3ce05da1a7a7d023</citedby><cites>FETCH-LOGICAL-c371t-861084f9fd18ea61f10e331d69548e9d0a0be30d575e102ec3ce05da1a7a7d023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38428617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Yun-Feng</creatorcontrib><creatorcontrib>Zhao, Ya-Qin</creatorcontrib><creatorcontrib>Zhou, Yang-yuntao</creatorcontrib><creatorcontrib>Feng, Hong-Yan</creatorcontrib><creatorcontrib>Gong, Lang-Lang</creatorcontrib><creatorcontrib>Zhang, Meng-Qi</creatorcontrib><creatorcontrib>Hull, J. Joe</creatorcontrib><creatorcontrib>Dewer, Youssef</creatorcontrib><creatorcontrib>Roy, Amit</creatorcontrib><creatorcontrib>Smagghe, Guy</creatorcontrib><creatorcontrib>He, Ming</creatorcontrib><creatorcontrib>He, Peng</creatorcontrib><title>Nanoparticle-delivered RNAi-based pesticide target screening for the rice pest white-backed planthopper and risk assessment for a natural predator</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Vacuolar-type (H+)-ATPase (vATPase) is a conserved multi-subunit eukaryotic enzyme composed of 14 subunits that form a functional complex consisting of an ATP-hydrolytic domain (V1) and a proton-translocation domain (V0). ATP hydrolysis and subsequent H+ translocation rely heavily on a fully assembled V1/V0 complex. Since vATPase is crucial for insect survival, it is a viable molecular target for pest control. However, detailed functional analyses of the 14 subunits and their suitability for pest control have not been fully explored in a single insect species. In this study, we identified 22 vATPase subunit transcripts that correspond to 13 subunits (A1, A2, B, C, D, E, F, G, H, a1, a2, c and d) in the white-backed planthopper (WBPH), Sogatella furcifera, a major hemipteran pest of rice. RNAi screens using microinjection and spray-based methods revealed that the SfVHA-F, SfVHA-a2 and SfVHA-c2 subunits are critical. Furthermore, star polymer (SPc) nanoparticles were utilized to conduct spray-induced and nanoparticle-delivered gene silencing (SI-NDGS) to evaluate the pest control efficacy of RNAi targeting the SfVHA-F, SfVHA-a2 and SfVHA-c2 transcripts. Target mRNA levels and vATPase enzymatic activity were both reduced. Honeydew excreta was likewise reduced in WBPH treated with dsRNAs targeting SfVHA-F, SfVHA-a2 and SfVHA-c2. To assess the environmental safety of the nanoparticle-wrapped dsRNAs, Cyrtorhinus lividipennis Reuter, a major natural enemy of planthoppers, was also sprayed with dsRNAs targeting SfVHA-F, SfVHA-a2 and SfVHA-c2. Post-spray effects of dsSfVHA-a2 and dsSfVHA-c2 on C. lividipennis were innocuous. This study identifies SfVHA-a2 and SfVHA-c2 as promising targets for biorational control of WBPH and lays the foundation for developing environment-friendly RNAi biopesticides.
[Display omitted]
•Twenty-two vATPase subunit transcripts were identified in Sogatella furcifera.•vATPase subunit transcripts were screened by RNAi as potential control targets.•Nanoparticle-delivery was used to increase the pest control efficacy.•Assessment of potential non-target organism effects in Cyrtorhinus lividipennis</description><subject>Adenosine Triphosphate</subject><subject>Animals</subject><subject>Hemiptera</subject><subject>Heteroptera</subject><subject>Natural enemy</subject><subject>Oryza - genetics</subject><subject>Pesticides</subject><subject>Risk Assessment</subject><subject>RNA Interference</subject><subject>Spray-induced and nanoparticle-delivered gene silencing</subject><subject>Star polymer</subject><subject>vATPase</subject><subject>White-backed planthopper</subject><issn>0048-9697</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkcFuGyEURVHUKHHS_kLLsptxYRgPzNKK2iRSlEpRskbP8MbGGcMUsKP-Rr44OE69LRuQOPfdC5eQb5xNOePtj_U0GZdDRr-b1qxuplzyWrUnZMKV7CrO6vYTmTDWqKprO3lOLlJas7Kk4mfkXKim0FxOyOs9-DBCzM4MWFkc3A4jWvpwP3fVAlI5jpjKrbNIM8QlZppMRPTOL2kfIs0rpNEZfOfoy8plLELzvFcO4PMqjCNGCt4WLD1TSAlT2qDP73KgHvI2wkDH4gs5xM_ktIch4ZeP_ZI8_fr5eHVT3f2-vr2a31VGSJ6rkp-ppu96yxVCy3vOUAhu227WKOwsA7ZAwexMzrD8BxphkM0scJAgLavFJfl-mDvG8GdbwuuNSwaHEhrDNum6E03dqpaJgsoDamJIKWKvx-g2EP9qzvS-EL3Wx0L0vhB9KKQov36YbBcbtEfdvwYKMD8AWJ66cxj3g9AbtC6iydoG91-TN6GTpDM</recordid><startdate>20240520</startdate><enddate>20240520</enddate><creator>Ma, Yun-Feng</creator><creator>Zhao, Ya-Qin</creator><creator>Zhou, Yang-yuntao</creator><creator>Feng, Hong-Yan</creator><creator>Gong, Lang-Lang</creator><creator>Zhang, Meng-Qi</creator><creator>Hull, J. Joe</creator><creator>Dewer, Youssef</creator><creator>Roy, Amit</creator><creator>Smagghe, Guy</creator><creator>He, Ming</creator><creator>He, Peng</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240520</creationdate><title>Nanoparticle-delivered RNAi-based pesticide target screening for the rice pest white-backed planthopper and risk assessment for a natural predator</title><author>Ma, Yun-Feng ; Zhao, Ya-Qin ; Zhou, Yang-yuntao ; Feng, Hong-Yan ; Gong, Lang-Lang ; Zhang, Meng-Qi ; Hull, J. Joe ; Dewer, Youssef ; Roy, Amit ; Smagghe, Guy ; He, Ming ; He, Peng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-861084f9fd18ea61f10e331d69548e9d0a0be30d575e102ec3ce05da1a7a7d023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adenosine Triphosphate</topic><topic>Animals</topic><topic>Hemiptera</topic><topic>Heteroptera</topic><topic>Natural enemy</topic><topic>Oryza - genetics</topic><topic>Pesticides</topic><topic>Risk Assessment</topic><topic>RNA Interference</topic><topic>Spray-induced and nanoparticle-delivered gene silencing</topic><topic>Star polymer</topic><topic>vATPase</topic><topic>White-backed planthopper</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Yun-Feng</creatorcontrib><creatorcontrib>Zhao, Ya-Qin</creatorcontrib><creatorcontrib>Zhou, Yang-yuntao</creatorcontrib><creatorcontrib>Feng, Hong-Yan</creatorcontrib><creatorcontrib>Gong, Lang-Lang</creatorcontrib><creatorcontrib>Zhang, Meng-Qi</creatorcontrib><creatorcontrib>Hull, J. Joe</creatorcontrib><creatorcontrib>Dewer, Youssef</creatorcontrib><creatorcontrib>Roy, Amit</creatorcontrib><creatorcontrib>Smagghe, Guy</creatorcontrib><creatorcontrib>He, Ming</creatorcontrib><creatorcontrib>He, Peng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Yun-Feng</au><au>Zhao, Ya-Qin</au><au>Zhou, Yang-yuntao</au><au>Feng, Hong-Yan</au><au>Gong, Lang-Lang</au><au>Zhang, Meng-Qi</au><au>Hull, J. Joe</au><au>Dewer, Youssef</au><au>Roy, Amit</au><au>Smagghe, Guy</au><au>He, Ming</au><au>He, Peng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanoparticle-delivered RNAi-based pesticide target screening for the rice pest white-backed planthopper and risk assessment for a natural predator</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2024-05-20</date><risdate>2024</risdate><volume>926</volume><spage>171286</spage><epage>171286</epage><pages>171286-171286</pages><artnum>171286</artnum><issn>0048-9697</issn><eissn>1879-1026</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Vacuolar-type (H+)-ATPase (vATPase) is a conserved multi-subunit eukaryotic enzyme composed of 14 subunits that form a functional complex consisting of an ATP-hydrolytic domain (V1) and a proton-translocation domain (V0). ATP hydrolysis and subsequent H+ translocation rely heavily on a fully assembled V1/V0 complex. Since vATPase is crucial for insect survival, it is a viable molecular target for pest control. However, detailed functional analyses of the 14 subunits and their suitability for pest control have not been fully explored in a single insect species. In this study, we identified 22 vATPase subunit transcripts that correspond to 13 subunits (A1, A2, B, C, D, E, F, G, H, a1, a2, c and d) in the white-backed planthopper (WBPH), Sogatella furcifera, a major hemipteran pest of rice. RNAi screens using microinjection and spray-based methods revealed that the SfVHA-F, SfVHA-a2 and SfVHA-c2 subunits are critical. Furthermore, star polymer (SPc) nanoparticles were utilized to conduct spray-induced and nanoparticle-delivered gene silencing (SI-NDGS) to evaluate the pest control efficacy of RNAi targeting the SfVHA-F, SfVHA-a2 and SfVHA-c2 transcripts. Target mRNA levels and vATPase enzymatic activity were both reduced. Honeydew excreta was likewise reduced in WBPH treated with dsRNAs targeting SfVHA-F, SfVHA-a2 and SfVHA-c2. To assess the environmental safety of the nanoparticle-wrapped dsRNAs, Cyrtorhinus lividipennis Reuter, a major natural enemy of planthoppers, was also sprayed with dsRNAs targeting SfVHA-F, SfVHA-a2 and SfVHA-c2. Post-spray effects of dsSfVHA-a2 and dsSfVHA-c2 on C. lividipennis were innocuous. This study identifies SfVHA-a2 and SfVHA-c2 as promising targets for biorational control of WBPH and lays the foundation for developing environment-friendly RNAi biopesticides.
[Display omitted]
•Twenty-two vATPase subunit transcripts were identified in Sogatella furcifera.•vATPase subunit transcripts were screened by RNAi as potential control targets.•Nanoparticle-delivery was used to increase the pest control efficacy.•Assessment of potential non-target organism effects in Cyrtorhinus lividipennis</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38428617</pmid><doi>10.1016/j.scitotenv.2024.171286</doi><tpages>1</tpages></addata></record> |
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| ispartof | The Science of the total environment, 2024-05, Vol.926, p.171286-171286, Article 171286 |
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| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Adenosine Triphosphate Animals Hemiptera Heteroptera Natural enemy Oryza - genetics Pesticides Risk Assessment RNA Interference Spray-induced and nanoparticle-delivered gene silencing Star polymer vATPase White-backed planthopper |
| title | Nanoparticle-delivered RNAi-based pesticide target screening for the rice pest white-backed planthopper and risk assessment for a natural predator |
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