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Integrative transcriptome and metabolome analysis reveals the discrepancy in the accumulation of active ingredients between Lycium barbarum cultivars
Main conclusion The combined analysis of transcriptome and metabolome provided molecular insight into the dynamics of multiple active ingredients biosynthesis and accumulation across different cultivars of Lycium barbarum . Lycium barbarum L. has a high concentration of active ingredients and is wel...
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| Published in: | Planta 2024-04, Vol.259 (4), p.74-74, Article 74 |
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| creator | Liu, Xuexia Gao, Han Radani, Yasmina Yue, Sijun Zhang, Ziping Tang, Jianning Zhu, Jinzhong Zheng, Rui |
| description | Main conclusion
The combined analysis of transcriptome and metabolome provided molecular insight into the dynamics of multiple active ingredients biosynthesis and accumulation across different cultivars of
Lycium barbarum
.
Lycium barbarum
L. has a high concentration of active ingredients and is well known in traditional Chinese herbal medicine for its therapeutic properties. However, there are many
Lycium barbarum
cultivars, and the content of active components varies, resulting in inconsistent quality between
Lycium barbarum
cultivars. At present, few research has been conducted to reveal the difference in active ingredient content among different cultivars of
Lycium barbarum
at the molecular level. Therefore, the transcriptome of 'Ningqi No.1' and 'Qixin No.1' during the three development stages (G, T, and M) was constructed in this study. A total of 797,570,278 clean reads were obtained. Between the two types of wolfberries, a total of 469, 2394, and 1531 differentially expressed genes (DEGs) were obtained in the ‘G1 vs. G10,’ ‘T1 vs. T10,’ and ‘M1 vs. M10,’ respectively, and were annotated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology identifiers. Using these transcriptome data, most DEGs related to the metabolism of the active ingredients in 'Ningqi No.1' and 'Qixin No.1' were identified. Moreover, a widely targeted metabolome analysis of the metabolites of 'Ningqi 1' and 'Qixin 1' fruits at the maturity stage revealed 1,135 differentially expressed metabolites (DEMs) in ‘M1 vs. M10,’ and many DEMs were associated with active ingredients such as flavonoids, alkaloids, terpenoids, and so on. We further quantified the flavonoid, lignin, and carotenoid contents of the two
Lycium barbarum
cultivars during the three developmental stages. The present outcome provided molecular insight into the dynamics of multiple active ingredients biosynthesis and accumulation across different cultivars of
Lycium barbarum
, which would provide the basic data for the formation of
Lycium barbarum
fruit quality and the breeding of outstanding strains. |
| doi_str_mv | 10.1007/s00425-024-04350-0 |
| format | article |
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The combined analysis of transcriptome and metabolome provided molecular insight into the dynamics of multiple active ingredients biosynthesis and accumulation across different cultivars of
Lycium barbarum
.
Lycium barbarum
L. has a high concentration of active ingredients and is well known in traditional Chinese herbal medicine for its therapeutic properties. However, there are many
Lycium barbarum
cultivars, and the content of active components varies, resulting in inconsistent quality between
Lycium barbarum
cultivars. At present, few research has been conducted to reveal the difference in active ingredient content among different cultivars of
Lycium barbarum
at the molecular level. Therefore, the transcriptome of 'Ningqi No.1' and 'Qixin No.1' during the three development stages (G, T, and M) was constructed in this study. A total of 797,570,278 clean reads were obtained. Between the two types of wolfberries, a total of 469, 2394, and 1531 differentially expressed genes (DEGs) were obtained in the ‘G1 vs. G10,’ ‘T1 vs. T10,’ and ‘M1 vs. M10,’ respectively, and were annotated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology identifiers. Using these transcriptome data, most DEGs related to the metabolism of the active ingredients in 'Ningqi No.1' and 'Qixin No.1' were identified. Moreover, a widely targeted metabolome analysis of the metabolites of 'Ningqi 1' and 'Qixin 1' fruits at the maturity stage revealed 1,135 differentially expressed metabolites (DEMs) in ‘M1 vs. M10,’ and many DEMs were associated with active ingredients such as flavonoids, alkaloids, terpenoids, and so on. We further quantified the flavonoid, lignin, and carotenoid contents of the two
Lycium barbarum
cultivars during the three developmental stages. The present outcome provided molecular insight into the dynamics of multiple active ingredients biosynthesis and accumulation across different cultivars of
Lycium barbarum
, which would provide the basic data for the formation of
Lycium barbarum
fruit quality and the breeding of outstanding strains.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-024-04350-0</identifier><identifier>PMID: 38407665</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Accumulation ; Agriculture ; Biomedical and Life Sciences ; Biosynthesis ; Carotenoids ; Cultivars ; Developmental stages ; Ecology ; Encyclopedias ; Flavonoids ; Flavonoids - genetics ; Forestry ; Fruits ; Genes ; Genomes ; Herbal medicine ; Ingredients ; Life Sciences ; Lycium - genetics ; Lycium barbarum ; Metabolites ; Metabolome ; Original Article ; Orthology ; Plant Breeding ; Plant Sciences ; Terpenes ; Traditional Chinese medicine ; Transcriptome - genetics ; Transcriptomes</subject><ispartof>Planta, 2024-04, Vol.259 (4), p.74-74, Article 74</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-7bb330c482d7498a76f1ac0a22ee1391a29d33eb15340cc37d41bc0e24cae9bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38407665$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Xuexia</creatorcontrib><creatorcontrib>Gao, Han</creatorcontrib><creatorcontrib>Radani, Yasmina</creatorcontrib><creatorcontrib>Yue, Sijun</creatorcontrib><creatorcontrib>Zhang, Ziping</creatorcontrib><creatorcontrib>Tang, Jianning</creatorcontrib><creatorcontrib>Zhu, Jinzhong</creatorcontrib><creatorcontrib>Zheng, Rui</creatorcontrib><title>Integrative transcriptome and metabolome analysis reveals the discrepancy in the accumulation of active ingredients between Lycium barbarum cultivars</title><title>Planta</title><addtitle>Planta</addtitle><addtitle>Planta</addtitle><description>Main conclusion
The combined analysis of transcriptome and metabolome provided molecular insight into the dynamics of multiple active ingredients biosynthesis and accumulation across different cultivars of
Lycium barbarum
.
Lycium barbarum
L. has a high concentration of active ingredients and is well known in traditional Chinese herbal medicine for its therapeutic properties. However, there are many
Lycium barbarum
cultivars, and the content of active components varies, resulting in inconsistent quality between
Lycium barbarum
cultivars. At present, few research has been conducted to reveal the difference in active ingredient content among different cultivars of
Lycium barbarum
at the molecular level. Therefore, the transcriptome of 'Ningqi No.1' and 'Qixin No.1' during the three development stages (G, T, and M) was constructed in this study. A total of 797,570,278 clean reads were obtained. Between the two types of wolfberries, a total of 469, 2394, and 1531 differentially expressed genes (DEGs) were obtained in the ‘G1 vs. G10,’ ‘T1 vs. T10,’ and ‘M1 vs. M10,’ respectively, and were annotated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology identifiers. Using these transcriptome data, most DEGs related to the metabolism of the active ingredients in 'Ningqi No.1' and 'Qixin No.1' were identified. Moreover, a widely targeted metabolome analysis of the metabolites of 'Ningqi 1' and 'Qixin 1' fruits at the maturity stage revealed 1,135 differentially expressed metabolites (DEMs) in ‘M1 vs. M10,’ and many DEMs were associated with active ingredients such as flavonoids, alkaloids, terpenoids, and so on. We further quantified the flavonoid, lignin, and carotenoid contents of the two
Lycium barbarum
cultivars during the three developmental stages. The present outcome provided molecular insight into the dynamics of multiple active ingredients biosynthesis and accumulation across different cultivars of
Lycium barbarum
, which would provide the basic data for the formation of
Lycium barbarum
fruit quality and the breeding of outstanding strains.</description><subject>Accumulation</subject><subject>Agriculture</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Carotenoids</subject><subject>Cultivars</subject><subject>Developmental stages</subject><subject>Ecology</subject><subject>Encyclopedias</subject><subject>Flavonoids</subject><subject>Flavonoids - genetics</subject><subject>Forestry</subject><subject>Fruits</subject><subject>Genes</subject><subject>Genomes</subject><subject>Herbal medicine</subject><subject>Ingredients</subject><subject>Life Sciences</subject><subject>Lycium - genetics</subject><subject>Lycium barbarum</subject><subject>Metabolites</subject><subject>Metabolome</subject><subject>Original Article</subject><subject>Orthology</subject><subject>Plant Breeding</subject><subject>Plant Sciences</subject><subject>Terpenes</subject><subject>Traditional Chinese medicine</subject><subject>Transcriptome - genetics</subject><subject>Transcriptomes</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kc-KFDEQxoMo7rj6Ah4k4MVLa-VPT7qPsqy6MOBFz6GSrhmzdKfHJL0yD-L7mp1eFTwIgVSlfvUVlY-xlwLeCgDzLgNo2TYgdQNatdDAI7YRWslGgu4esw1AjaFX7QV7lvMtQC0a85RdqE6D2W7bDft5EwsdEpZwR7wkjNmncCzzRBzjwCcq6OZxTXE85ZB5ojvCMfPyjfgQKk9HjP7EQzw_offLtIxVcY583tf8rB3iIdEQKJbMHZUfRJHvTj4sE3eY6qmBX8bKYsrP2ZN9HUEvHu5L9vXD9ZerT83u88ebq_e7xiu5LY1xTinwupOD0X2HZrsX6AGlJBKqFyj7QSlyolUavFdm0MJ5IKk9Uu8GdcnerLrHNH9fKBc71YVoHDHSvGQreyVBtKZrK_r6H_R2XlL9kzMlutaAgErJlfJpzjnR3h5TmDCdrAB7b5pdTbPVNHs2zd43vXqQXtxEw5-W3y5VQK1ArqV4oPR39n9kfwHiGKW4</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Liu, Xuexia</creator><creator>Gao, Han</creator><creator>Radani, Yasmina</creator><creator>Yue, Sijun</creator><creator>Zhang, Ziping</creator><creator>Tang, Jianning</creator><creator>Zhu, Jinzhong</creator><creator>Zheng, Rui</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20240401</creationdate><title>Integrative transcriptome and metabolome analysis reveals the discrepancy in the accumulation of active ingredients between Lycium barbarum cultivars</title><author>Liu, Xuexia ; Gao, Han ; Radani, Yasmina ; Yue, Sijun ; Zhang, Ziping ; Tang, Jianning ; Zhu, Jinzhong ; Zheng, Rui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-7bb330c482d7498a76f1ac0a22ee1391a29d33eb15340cc37d41bc0e24cae9bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Accumulation</topic><topic>Agriculture</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Carotenoids</topic><topic>Cultivars</topic><topic>Developmental stages</topic><topic>Ecology</topic><topic>Encyclopedias</topic><topic>Flavonoids</topic><topic>Flavonoids - genetics</topic><topic>Forestry</topic><topic>Fruits</topic><topic>Genes</topic><topic>Genomes</topic><topic>Herbal medicine</topic><topic>Ingredients</topic><topic>Life Sciences</topic><topic>Lycium - genetics</topic><topic>Lycium barbarum</topic><topic>Metabolites</topic><topic>Metabolome</topic><topic>Original Article</topic><topic>Orthology</topic><topic>Plant Breeding</topic><topic>Plant Sciences</topic><topic>Terpenes</topic><topic>Traditional Chinese medicine</topic><topic>Transcriptome - genetics</topic><topic>Transcriptomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Xuexia</creatorcontrib><creatorcontrib>Gao, Han</creatorcontrib><creatorcontrib>Radani, Yasmina</creatorcontrib><creatorcontrib>Yue, Sijun</creatorcontrib><creatorcontrib>Zhang, Ziping</creatorcontrib><creatorcontrib>Tang, Jianning</creatorcontrib><creatorcontrib>Zhu, Jinzhong</creatorcontrib><creatorcontrib>Zheng, Rui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Xuexia</au><au>Gao, Han</au><au>Radani, Yasmina</au><au>Yue, Sijun</au><au>Zhang, Ziping</au><au>Tang, Jianning</au><au>Zhu, Jinzhong</au><au>Zheng, Rui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrative transcriptome and metabolome analysis reveals the discrepancy in the accumulation of active ingredients between Lycium barbarum cultivars</atitle><jtitle>Planta</jtitle><stitle>Planta</stitle><addtitle>Planta</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>259</volume><issue>4</issue><spage>74</spage><epage>74</epage><pages>74-74</pages><artnum>74</artnum><issn>0032-0935</issn><eissn>1432-2048</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Main conclusion
The combined analysis of transcriptome and metabolome provided molecular insight into the dynamics of multiple active ingredients biosynthesis and accumulation across different cultivars of
Lycium barbarum
.
Lycium barbarum
L. has a high concentration of active ingredients and is well known in traditional Chinese herbal medicine for its therapeutic properties. However, there are many
Lycium barbarum
cultivars, and the content of active components varies, resulting in inconsistent quality between
Lycium barbarum
cultivars. At present, few research has been conducted to reveal the difference in active ingredient content among different cultivars of
Lycium barbarum
at the molecular level. Therefore, the transcriptome of 'Ningqi No.1' and 'Qixin No.1' during the three development stages (G, T, and M) was constructed in this study. A total of 797,570,278 clean reads were obtained. Between the two types of wolfberries, a total of 469, 2394, and 1531 differentially expressed genes (DEGs) were obtained in the ‘G1 vs. G10,’ ‘T1 vs. T10,’ and ‘M1 vs. M10,’ respectively, and were annotated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology identifiers. Using these transcriptome data, most DEGs related to the metabolism of the active ingredients in 'Ningqi No.1' and 'Qixin No.1' were identified. Moreover, a widely targeted metabolome analysis of the metabolites of 'Ningqi 1' and 'Qixin 1' fruits at the maturity stage revealed 1,135 differentially expressed metabolites (DEMs) in ‘M1 vs. M10,’ and many DEMs were associated with active ingredients such as flavonoids, alkaloids, terpenoids, and so on. We further quantified the flavonoid, lignin, and carotenoid contents of the two
Lycium barbarum
cultivars during the three developmental stages. The present outcome provided molecular insight into the dynamics of multiple active ingredients biosynthesis and accumulation across different cultivars of
Lycium barbarum
, which would provide the basic data for the formation of
Lycium barbarum
fruit quality and the breeding of outstanding strains.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38407665</pmid><doi>10.1007/s00425-024-04350-0</doi><tpages>1</tpages></addata></record> |
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| subjects | Accumulation Agriculture Biomedical and Life Sciences Biosynthesis Carotenoids Cultivars Developmental stages Ecology Encyclopedias Flavonoids Flavonoids - genetics Forestry Fruits Genes Genomes Herbal medicine Ingredients Life Sciences Lycium - genetics Lycium barbarum Metabolites Metabolome Original Article Orthology Plant Breeding Plant Sciences Terpenes Traditional Chinese medicine Transcriptome - genetics Transcriptomes |
| title | Integrative transcriptome and metabolome analysis reveals the discrepancy in the accumulation of active ingredients between Lycium barbarum cultivars |
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