Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model

Hepatic cancer is among the most recurrently detected malignancies worldwide and one of the main contributors to cancer-associated mortality. With few available therapeutic choices, there is an instant necessity to explore suitable options. In this aspect, Nanotechnology has been employed to explore...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2024-01, Vol.256 (Pt 2), p.127490-127490, Article 127490
Main Authors: Elderdery, Abozer Y., Alzerwi, Nasser A.N., Alzahrani, Badr, Alsrhani, Abdullah, Alsultan, Afnan, Rayzah, Musaed, Idrees, Bandar, Rayzah, Fares, Baksh, Yaser, Alzahrani, Ahmed M., Alabdulsalam, Abdulrahim A., Mohamedain, A., Subbiah, Suresh Kumar, Mok, Pooi Ling
Format: Article
Language:English
Subjects:
Alginates - pharmacology
Animals
Apoptosis
Cell Line, Tumor
Chemical and Drug Induced Liver Injury
Eugenol - pharmacology
Ferric Compounds
Hep3B cell line
Liver cancer
Nanocomposites - chemistry
Nanotechnology
Phosphatidylinositol 3-Kinases - metabolism
Prospective Studies
Proto-Oncogene Proteins c-akt - metabolism
TOR Serine-Threonine Kinases - metabolism
zebra fishes
Zebrafish - metabolism
α-Fe2O3–sodium alginate-eugenol nanocomposites
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c315t-ca626177740b3e182e25c533e27e9bd55385dcecc912fab916fe7abd8516c9a13
container_end_page 127490
container_issue Pt 2
container_start_page 127490
container_title International journal of biological macromolecules
container_volume 256
creator Elderdery, Abozer Y.
Alzerwi, Nasser A.N.
Alzahrani, Badr
Alsrhani, Abdullah
Alsultan, Afnan
Rayzah, Musaed
Idrees, Bandar
Rayzah, Fares
Baksh, Yaser
Alzahrani, Ahmed M.
Alabdulsalam, Abdulrahim A.
Mohamedain, A.
Subbiah, Suresh Kumar
Mok, Pooi Ling
description Hepatic cancer is among the most recurrently detected malignancies worldwide and one of the main contributors to cancer-associated mortality. With few available therapeutic choices, there is an instant necessity to explore suitable options. In this aspect, Nanotechnology has been employed to explore prospective chemotherapeutic approaches, especially for cancer treatment. Nanotechnology is concerned with the biological and physical properties of nanoparticles in the therapeutic use of drugs. In the current work, formulation, and characterization of α-Fe2O3–Sodium Alginate-Eugenol nanocomposites (FSE NCs) using several approaches like SEM and TEM, UV–visible, FTIR, and PL spectroscopy, XRD, EDAX, and DLS studies have been performed. With an average size of 50 nm, the rhombohedral structure of NCs was identified. Further, their anticancer activity against Hep3B liver cancer cell lines has been performed by cell viability, dual staining, DCFH-DA, Annexin-V/-FITC/PI, cell cycle analysis methods, and PI3K/Akt/mTOR signaling proteins were studied to assess the anticancer effects of the NCs in Hep3B cells. Also, anti-cancer activity on animal modeling in-vivo using zebra fishes to hematological parameters, liver enzymes, and histopathology study effectiveness was noticed. Moreover, the NCs reduced the viability, elevated the ROS accumulation, diminished the membrane integrity, reduced the antioxidants, blocked the cell cycle, and triggered the PI3K/Akt/mTOR signaling axis that eventually resulted in cell death. As a result, FSE NCs possess huge potential for use as a possible anticancer candidate.
doi_str_mv 10.1016/j.ijbiomac.2023.127490
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2891753491</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0141813023043878</els_id><sourcerecordid>2891753491</sourcerecordid><originalsourceid>FETCH-LOGICAL-c315t-ca626177740b3e182e25c533e27e9bd55385dcecc912fab916fe7abd8516c9a13</originalsourceid><addsrcrecordid>eNqFkc1u1DAUhS0EotPSV6i8ZEFm7Hicnx1VVWjViiLUri3Hvpm5Q2KH2BlRXqivicO03aK7uNLRd-6xdQg542zJGS9WuyXuGvS9Nsuc5WLJ83Jdszdkwauyzhhj4i1ZML7mWcUFOyLHIeySWkhevSdHoqzTyGpBnr5p543vBx8wQqC-pTh6R_1vtPCJBm9x6qnuNuh0TIJ2lsK0Aec7is5OBqge_BCTPdA9avr9Wtyszn_GVX9_94MG3DjdodskmF7BIKiBrgv_ziRlj3tPtzDo6GNKNBgfZzlugf6BZtQthi3tvYXuA3nX6i7A6fM-IQ9fLu8vrrLbu6_XF-e3mRFcxszoIi94WZZr1gjgVQ65NFIIyEuoGyulqKQ1YEzN81Y3NS9aKHVjK8kLU2suTsjHw91h9L8mCFH1GOY3awd-Ciqval5Ksa5ntDigZvQhjNCqYcRej4-KMzWXpHbqpSQ1l6QOJSXj2XPG1PRgX20vrSTg8wGA9NM9wqiCQXAGLI5gorIe_5fxFyTmqMo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2891753491</pqid></control><display><type>article</type><title>Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model</title><source>ScienceDirect Journals</source><creator>Elderdery, Abozer Y. ; Alzerwi, Nasser A.N. ; Alzahrani, Badr ; Alsrhani, Abdullah ; Alsultan, Afnan ; Rayzah, Musaed ; Idrees, Bandar ; Rayzah, Fares ; Baksh, Yaser ; Alzahrani, Ahmed M. ; Alabdulsalam, Abdulrahim A. ; Mohamedain, A. ; Subbiah, Suresh Kumar ; Mok, Pooi Ling</creator><creatorcontrib>Elderdery, Abozer Y. ; Alzerwi, Nasser A.N. ; Alzahrani, Badr ; Alsrhani, Abdullah ; Alsultan, Afnan ; Rayzah, Musaed ; Idrees, Bandar ; Rayzah, Fares ; Baksh, Yaser ; Alzahrani, Ahmed M. ; Alabdulsalam, Abdulrahim A. ; Mohamedain, A. ; Subbiah, Suresh Kumar ; Mok, Pooi Ling</creatorcontrib><description>Hepatic cancer is among the most recurrently detected malignancies worldwide and one of the main contributors to cancer-associated mortality. With few available therapeutic choices, there is an instant necessity to explore suitable options. In this aspect, Nanotechnology has been employed to explore prospective chemotherapeutic approaches, especially for cancer treatment. Nanotechnology is concerned with the biological and physical properties of nanoparticles in the therapeutic use of drugs. In the current work, formulation, and characterization of α-Fe2O3–Sodium Alginate-Eugenol nanocomposites (FSE NCs) using several approaches like SEM and TEM, UV–visible, FTIR, and PL spectroscopy, XRD, EDAX, and DLS studies have been performed. With an average size of 50 nm, the rhombohedral structure of NCs was identified. Further, their anticancer activity against Hep3B liver cancer cell lines has been performed by cell viability, dual staining, DCFH-DA, Annexin-V/-FITC/PI, cell cycle analysis methods, and PI3K/Akt/mTOR signaling proteins were studied to assess the anticancer effects of the NCs in Hep3B cells. Also, anti-cancer activity on animal modeling in-vivo using zebra fishes to hematological parameters, liver enzymes, and histopathology study effectiveness was noticed. Moreover, the NCs reduced the viability, elevated the ROS accumulation, diminished the membrane integrity, reduced the antioxidants, blocked the cell cycle, and triggered the PI3K/Akt/mTOR signaling axis that eventually resulted in cell death. As a result, FSE NCs possess huge potential for use as a possible anticancer candidate.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2023.127490</identifier><identifier>PMID: 37979758</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alginates - pharmacology ; Animals ; Apoptosis ; Cell Line, Tumor ; Chemical and Drug Induced Liver Injury ; Eugenol - pharmacology ; Ferric Compounds ; Hep3B cell line ; Liver cancer ; Nanocomposites - chemistry ; Nanotechnology ; Phosphatidylinositol 3-Kinases - metabolism ; Prospective Studies ; Proto-Oncogene Proteins c-akt - metabolism ; TOR Serine-Threonine Kinases - metabolism ; zebra fishes ; Zebrafish - metabolism ; α-Fe2O3–sodium alginate-eugenol nanocomposites</subject><ispartof>International journal of biological macromolecules, 2024-01, Vol.256 (Pt 2), p.127490-127490, Article 127490</ispartof><rights>2023</rights><rights>Copyright © 2023. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c315t-ca626177740b3e182e25c533e27e9bd55385dcecc912fab916fe7abd8516c9a13</cites><orcidid>0000-0002-6219-5312</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37979758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elderdery, Abozer Y.</creatorcontrib><creatorcontrib>Alzerwi, Nasser A.N.</creatorcontrib><creatorcontrib>Alzahrani, Badr</creatorcontrib><creatorcontrib>Alsrhani, Abdullah</creatorcontrib><creatorcontrib>Alsultan, Afnan</creatorcontrib><creatorcontrib>Rayzah, Musaed</creatorcontrib><creatorcontrib>Idrees, Bandar</creatorcontrib><creatorcontrib>Rayzah, Fares</creatorcontrib><creatorcontrib>Baksh, Yaser</creatorcontrib><creatorcontrib>Alzahrani, Ahmed M.</creatorcontrib><creatorcontrib>Alabdulsalam, Abdulrahim A.</creatorcontrib><creatorcontrib>Mohamedain, A.</creatorcontrib><creatorcontrib>Subbiah, Suresh Kumar</creatorcontrib><creatorcontrib>Mok, Pooi Ling</creatorcontrib><title>Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Hepatic cancer is among the most recurrently detected malignancies worldwide and one of the main contributors to cancer-associated mortality. With few available therapeutic choices, there is an instant necessity to explore suitable options. In this aspect, Nanotechnology has been employed to explore prospective chemotherapeutic approaches, especially for cancer treatment. Nanotechnology is concerned with the biological and physical properties of nanoparticles in the therapeutic use of drugs. In the current work, formulation, and characterization of α-Fe2O3–Sodium Alginate-Eugenol nanocomposites (FSE NCs) using several approaches like SEM and TEM, UV–visible, FTIR, and PL spectroscopy, XRD, EDAX, and DLS studies have been performed. With an average size of 50 nm, the rhombohedral structure of NCs was identified. Further, their anticancer activity against Hep3B liver cancer cell lines has been performed by cell viability, dual staining, DCFH-DA, Annexin-V/-FITC/PI, cell cycle analysis methods, and PI3K/Akt/mTOR signaling proteins were studied to assess the anticancer effects of the NCs in Hep3B cells. Also, anti-cancer activity on animal modeling in-vivo using zebra fishes to hematological parameters, liver enzymes, and histopathology study effectiveness was noticed. Moreover, the NCs reduced the viability, elevated the ROS accumulation, diminished the membrane integrity, reduced the antioxidants, blocked the cell cycle, and triggered the PI3K/Akt/mTOR signaling axis that eventually resulted in cell death. As a result, FSE NCs possess huge potential for use as a possible anticancer candidate.</description><subject>Alginates - pharmacology</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cell Line, Tumor</subject><subject>Chemical and Drug Induced Liver Injury</subject><subject>Eugenol - pharmacology</subject><subject>Ferric Compounds</subject><subject>Hep3B cell line</subject><subject>Liver cancer</subject><subject>Nanocomposites - chemistry</subject><subject>Nanotechnology</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Prospective Studies</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>TOR Serine-Threonine Kinases - metabolism</subject><subject>zebra fishes</subject><subject>Zebrafish - metabolism</subject><subject>α-Fe2O3–sodium alginate-eugenol nanocomposites</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u1DAUhS0EotPSV6i8ZEFm7Hicnx1VVWjViiLUri3Hvpm5Q2KH2BlRXqivicO03aK7uNLRd-6xdQg542zJGS9WuyXuGvS9Nsuc5WLJ83Jdszdkwauyzhhj4i1ZML7mWcUFOyLHIeySWkhevSdHoqzTyGpBnr5p543vBx8wQqC-pTh6R_1vtPCJBm9x6qnuNuh0TIJ2lsK0Aec7is5OBqge_BCTPdA9avr9Wtyszn_GVX9_94MG3DjdodskmF7BIKiBrgv_ziRlj3tPtzDo6GNKNBgfZzlugf6BZtQthi3tvYXuA3nX6i7A6fM-IQ9fLu8vrrLbu6_XF-e3mRFcxszoIi94WZZr1gjgVQ65NFIIyEuoGyulqKQ1YEzN81Y3NS9aKHVjK8kLU2suTsjHw91h9L8mCFH1GOY3awd-Ciqval5Ksa5ntDigZvQhjNCqYcRej4-KMzWXpHbqpSQ1l6QOJSXj2XPG1PRgX20vrSTg8wGA9NM9wqiCQXAGLI5gorIe_5fxFyTmqMo</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Elderdery, Abozer Y.</creator><creator>Alzerwi, Nasser A.N.</creator><creator>Alzahrani, Badr</creator><creator>Alsrhani, Abdullah</creator><creator>Alsultan, Afnan</creator><creator>Rayzah, Musaed</creator><creator>Idrees, Bandar</creator><creator>Rayzah, Fares</creator><creator>Baksh, Yaser</creator><creator>Alzahrani, Ahmed M.</creator><creator>Alabdulsalam, Abdulrahim A.</creator><creator>Mohamedain, A.</creator><creator>Subbiah, Suresh Kumar</creator><creator>Mok, Pooi Ling</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6219-5312</orcidid></search><sort><creationdate>202401</creationdate><title>Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model</title><author>Elderdery, Abozer Y. ; Alzerwi, Nasser A.N. ; Alzahrani, Badr ; Alsrhani, Abdullah ; Alsultan, Afnan ; Rayzah, Musaed ; Idrees, Bandar ; Rayzah, Fares ; Baksh, Yaser ; Alzahrani, Ahmed M. ; Alabdulsalam, Abdulrahim A. ; Mohamedain, A. ; Subbiah, Suresh Kumar ; Mok, Pooi Ling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-ca626177740b3e182e25c533e27e9bd55385dcecc912fab916fe7abd8516c9a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Alginates - pharmacology</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cell Line, Tumor</topic><topic>Chemical and Drug Induced Liver Injury</topic><topic>Eugenol - pharmacology</topic><topic>Ferric Compounds</topic><topic>Hep3B cell line</topic><topic>Liver cancer</topic><topic>Nanocomposites - chemistry</topic><topic>Nanotechnology</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Prospective Studies</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>TOR Serine-Threonine Kinases - metabolism</topic><topic>zebra fishes</topic><topic>Zebrafish - metabolism</topic><topic>α-Fe2O3–sodium alginate-eugenol nanocomposites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Elderdery, Abozer Y.</creatorcontrib><creatorcontrib>Alzerwi, Nasser A.N.</creatorcontrib><creatorcontrib>Alzahrani, Badr</creatorcontrib><creatorcontrib>Alsrhani, Abdullah</creatorcontrib><creatorcontrib>Alsultan, Afnan</creatorcontrib><creatorcontrib>Rayzah, Musaed</creatorcontrib><creatorcontrib>Idrees, Bandar</creatorcontrib><creatorcontrib>Rayzah, Fares</creatorcontrib><creatorcontrib>Baksh, Yaser</creatorcontrib><creatorcontrib>Alzahrani, Ahmed M.</creatorcontrib><creatorcontrib>Alabdulsalam, Abdulrahim A.</creatorcontrib><creatorcontrib>Mohamedain, A.</creatorcontrib><creatorcontrib>Subbiah, Suresh Kumar</creatorcontrib><creatorcontrib>Mok, Pooi Ling</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elderdery, Abozer Y.</au><au>Alzerwi, Nasser A.N.</au><au>Alzahrani, Badr</au><au>Alsrhani, Abdullah</au><au>Alsultan, Afnan</au><au>Rayzah, Musaed</au><au>Idrees, Bandar</au><au>Rayzah, Fares</au><au>Baksh, Yaser</au><au>Alzahrani, Ahmed M.</au><au>Alabdulsalam, Abdulrahim A.</au><au>Mohamedain, A.</au><au>Subbiah, Suresh Kumar</au><au>Mok, Pooi Ling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2024-01</date><risdate>2024</risdate><volume>256</volume><issue>Pt 2</issue><spage>127490</spage><epage>127490</epage><pages>127490-127490</pages><artnum>127490</artnum><issn>0141-8130</issn><eissn>1879-0003</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Hepatic cancer is among the most recurrently detected malignancies worldwide and one of the main contributors to cancer-associated mortality. With few available therapeutic choices, there is an instant necessity to explore suitable options. In this aspect, Nanotechnology has been employed to explore prospective chemotherapeutic approaches, especially for cancer treatment. Nanotechnology is concerned with the biological and physical properties of nanoparticles in the therapeutic use of drugs. In the current work, formulation, and characterization of α-Fe2O3–Sodium Alginate-Eugenol nanocomposites (FSE NCs) using several approaches like SEM and TEM, UV–visible, FTIR, and PL spectroscopy, XRD, EDAX, and DLS studies have been performed. With an average size of 50 nm, the rhombohedral structure of NCs was identified. Further, their anticancer activity against Hep3B liver cancer cell lines has been performed by cell viability, dual staining, DCFH-DA, Annexin-V/-FITC/PI, cell cycle analysis methods, and PI3K/Akt/mTOR signaling proteins were studied to assess the anticancer effects of the NCs in Hep3B cells. Also, anti-cancer activity on animal modeling in-vivo using zebra fishes to hematological parameters, liver enzymes, and histopathology study effectiveness was noticed. Moreover, the NCs reduced the viability, elevated the ROS accumulation, diminished the membrane integrity, reduced the antioxidants, blocked the cell cycle, and triggered the PI3K/Akt/mTOR signaling axis that eventually resulted in cell death. As a result, FSE NCs possess huge potential for use as a possible anticancer candidate.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>37979758</pmid><doi>10.1016/j.ijbiomac.2023.127490</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-6219-5312</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0141-8130
ispartof International journal of biological macromolecules, 2024-01, Vol.256 (Pt 2), p.127490-127490, Article 127490
issn 0141-8130
1879-0003
language eng
recordid cdi_proquest_miscellaneous_2891753491
source ScienceDirect Journals
subjects Alginates - pharmacology
Animals
Apoptosis
Cell Line, Tumor
Chemical and Drug Induced Liver Injury
Eugenol - pharmacology
Ferric Compounds
Hep3B cell line
Liver cancer
Nanocomposites - chemistry
Nanotechnology
Phosphatidylinositol 3-Kinases - metabolism
Prospective Studies
Proto-Oncogene Proteins c-akt - metabolism
TOR Serine-Threonine Kinases - metabolism
zebra fishes
Zebrafish - metabolism
α-Fe2O3–sodium alginate-eugenol nanocomposites
title Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T14%3A26%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nanocomposites%20of%20iron%20oxide,%20sodium%20alginate,%20and%20eugenol%20induce%20apoptosis%20via%20PI3K/Akt/mTOR%20signaling%20in%20Hep3%20cells%20and%20in%20vivo%20hepatotoxicity%20in%20the%20zebrafish%20model&rft.jtitle=International%20journal%20of%20biological%20macromolecules&rft.au=Elderdery,%20Abozer%20Y.&rft.date=2024-01&rft.volume=256&rft.issue=Pt%202&rft.spage=127490&rft.epage=127490&rft.pages=127490-127490&rft.artnum=127490&rft.issn=0141-8130&rft.eissn=1879-0003&rft_id=info:doi/10.1016/j.ijbiomac.2023.127490&rft_dat=%3Cproquest_cross%3E2891753491%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c315t-ca626177740b3e182e25c533e27e9bd55385dcecc912fab916fe7abd8516c9a13%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2891753491&rft_id=info:pmid/37979758&rfr_iscdi=true