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Surface modification of a screen-printed electrode with a flower-like nanostructure to fabricate a guanine DNA-based electrochemical biosensor to determine the anticancer drug pemigatinib
The present study developed a DNA biosensor to determine pemigatinib for the first time. Three-dimensional carnation flower-like Eu 3+ :β-MnO 2 nanostructures (3D CF-L Eu 3+ :β-MnO 2 NSs) and a screen-printed electrode (SPE) modified with polyaniline (PA) were employed. The double-stranded DNA was a...
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| Published in: | Analytical methods 2023-10, Vol.15 (39), p.5146-5156 |
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| container_end_page | 5156 |
| container_issue | 39 |
| container_start_page | 5146 |
| container_title | Analytical methods |
| container_volume | 15 |
| creator | Al-Qargholi, Basim Al-dolaimy, F Altalbawy, Farag M. A Kadhim, Abed J Alsaalamy, Ali Hashiem Suliman, Muath Abbas, Ahmed hussien R |
| description | The present study developed a DNA biosensor to determine pemigatinib for the first time. Three-dimensional carnation flower-like Eu
3+
:β-MnO
2
nanostructures (3D CF-L Eu
3+
:β-MnO
2
NSs) and a screen-printed electrode (SPE) modified with polyaniline (PA) were employed. The double-stranded DNA was also immobilized completely on the PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE. Then, electrochemical techniques were used for characterizing the modified electrode. After that, the interaction between pemigatinib and DNA was shown by a reduction in the oxidation current of guanine using differential pulse voltammetry (DPV). According to the analysis, the dynamic range of pemigatinib was between 0.001 and 180.0 μM, indicating the new electrode has a low limit of detection (LOD = 0.23 nM) for pemigatinib. Afterwards, pemigatinib in real samples was measured using the PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE loaded with ds-DNA. The proposed DNA biosensor showed good selectivity toward pemigatinib in the presence of other interference analytes, such as other ions, structurally related pharmaceuticals, and plasma proteins. In addition, the interaction site of pemigatinib with DNA was predicted by molecular docking, which showed the interaction of pemigatinib with the guanine bases of DNA through a groove binding mode. Finally, we employed the
t
-test to verify the capability of the ds-DNA/PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE for analyzing pemigatinib in real samples.
The present study developed a DNA biosensor to determine pemigatinib for the first time. |
| doi_str_mv | 10.1039/d3ay01103h |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2869615608</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2875893716</sourcerecordid><originalsourceid>FETCH-LOGICAL-c188t-7cf8937285fe5e87deeb9225b2285d6a9b7567693528dd9088f79240c93fcecd3</originalsourceid><addsrcrecordid>eNpdkU9LHTEUxYfSQq3tpvtCwE0pTE1mOvmzfGjVguhCu-hqyCQ370VnkudNBvGz-eXM-EShq1xyf-dwuKeqvjL6k9FWHdpWP1BWxs27ao-JTtWKC_X-deb0Y_UppRtKuWo526ser2Z02gCZovXOG519DCQ6okkyCBDqLfqQwRIYwWSMFsi9z5uyd2O8B6xHfwsk6BBTxtnkGYHkSJwecHGDAq5nHXwAcnyxqged3rzMBqYCjWTwMUFIERephQw4LYK8KfKQCxIMILE4r8m2SNYlZfDD5-qD02OCLy_vfvX35Pf10Vl9fnn652h1XhsmZa6FcVK1opGdgw6ksACDappuaMqX5VoNouOi3KNrpLWKSumEan5Ro1pnwNh2v_q-891ivJsh5X7yycA46gBxTn0jueKs41QW9OA_9CbOGEq6QoluycF4oX7sKIMxJQTXlyNPGh96Rvulx_64Xf177vGswN92MCbzyr313D4BalieMg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2875893716</pqid></control><display><type>article</type><title>Surface modification of a screen-printed electrode with a flower-like nanostructure to fabricate a guanine DNA-based electrochemical biosensor to determine the anticancer drug pemigatinib</title><source>Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list)</source><creator>Al-Qargholi, Basim ; Al-dolaimy, F ; Altalbawy, Farag M. A ; Kadhim, Abed J ; Alsaalamy, Ali Hashiem ; Suliman, Muath ; Abbas, Ahmed hussien R</creator><creatorcontrib>Al-Qargholi, Basim ; Al-dolaimy, F ; Altalbawy, Farag M. A ; Kadhim, Abed J ; Alsaalamy, Ali Hashiem ; Suliman, Muath ; Abbas, Ahmed hussien R</creatorcontrib><description>The present study developed a DNA biosensor to determine pemigatinib for the first time. Three-dimensional carnation flower-like Eu
3+
:β-MnO
2
nanostructures (3D CF-L Eu
3+
:β-MnO
2
NSs) and a screen-printed electrode (SPE) modified with polyaniline (PA) were employed. The double-stranded DNA was also immobilized completely on the PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE. Then, electrochemical techniques were used for characterizing the modified electrode. After that, the interaction between pemigatinib and DNA was shown by a reduction in the oxidation current of guanine using differential pulse voltammetry (DPV). According to the analysis, the dynamic range of pemigatinib was between 0.001 and 180.0 μM, indicating the new electrode has a low limit of detection (LOD = 0.23 nM) for pemigatinib. Afterwards, pemigatinib in real samples was measured using the PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE loaded with ds-DNA. The proposed DNA biosensor showed good selectivity toward pemigatinib in the presence of other interference analytes, such as other ions, structurally related pharmaceuticals, and plasma proteins. In addition, the interaction site of pemigatinib with DNA was predicted by molecular docking, which showed the interaction of pemigatinib with the guanine bases of DNA through a groove binding mode. Finally, we employed the
t
-test to verify the capability of the ds-DNA/PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE for analyzing pemigatinib in real samples.
The present study developed a DNA biosensor to determine pemigatinib for the first time.</description><identifier>ISSN: 1759-9660</identifier><identifier>EISSN: 1759-9679</identifier><identifier>DOI: 10.1039/d3ay01103h</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Antitumor agents ; Biosensors ; Deoxyribonucleic acid ; DNA ; Electrochemistry ; Electrodes ; Electrons ; Europium ; Grooves ; Guanine ; Manganese dioxide ; Molecular docking ; Nanostructure ; Oxidation ; Plasma proteins ; Polyanilines ; Three dimensional flow</subject><ispartof>Analytical methods, 2023-10, Vol.15 (39), p.5146-5156</ispartof><rights>Copyright Royal Society of Chemistry 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c188t-7cf8937285fe5e87deeb9225b2285d6a9b7567693528dd9088f79240c93fcecd3</cites><orcidid>0009-0004-8854-6151</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids></links><search><creatorcontrib>Al-Qargholi, Basim</creatorcontrib><creatorcontrib>Al-dolaimy, F</creatorcontrib><creatorcontrib>Altalbawy, Farag M. A</creatorcontrib><creatorcontrib>Kadhim, Abed J</creatorcontrib><creatorcontrib>Alsaalamy, Ali Hashiem</creatorcontrib><creatorcontrib>Suliman, Muath</creatorcontrib><creatorcontrib>Abbas, Ahmed hussien R</creatorcontrib><title>Surface modification of a screen-printed electrode with a flower-like nanostructure to fabricate a guanine DNA-based electrochemical biosensor to determine the anticancer drug pemigatinib</title><title>Analytical methods</title><description>The present study developed a DNA biosensor to determine pemigatinib for the first time. Three-dimensional carnation flower-like Eu
3+
:β-MnO
2
nanostructures (3D CF-L Eu
3+
:β-MnO
2
NSs) and a screen-printed electrode (SPE) modified with polyaniline (PA) were employed. The double-stranded DNA was also immobilized completely on the PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE. Then, electrochemical techniques were used for characterizing the modified electrode. After that, the interaction between pemigatinib and DNA was shown by a reduction in the oxidation current of guanine using differential pulse voltammetry (DPV). According to the analysis, the dynamic range of pemigatinib was between 0.001 and 180.0 μM, indicating the new electrode has a low limit of detection (LOD = 0.23 nM) for pemigatinib. Afterwards, pemigatinib in real samples was measured using the PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE loaded with ds-DNA. The proposed DNA biosensor showed good selectivity toward pemigatinib in the presence of other interference analytes, such as other ions, structurally related pharmaceuticals, and plasma proteins. In addition, the interaction site of pemigatinib with DNA was predicted by molecular docking, which showed the interaction of pemigatinib with the guanine bases of DNA through a groove binding mode. Finally, we employed the
t
-test to verify the capability of the ds-DNA/PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE for analyzing pemigatinib in real samples.
The present study developed a DNA biosensor to determine pemigatinib for the first time.</description><subject>Antitumor agents</subject><subject>Biosensors</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Electrons</subject><subject>Europium</subject><subject>Grooves</subject><subject>Guanine</subject><subject>Manganese dioxide</subject><subject>Molecular docking</subject><subject>Nanostructure</subject><subject>Oxidation</subject><subject>Plasma proteins</subject><subject>Polyanilines</subject><subject>Three dimensional flow</subject><issn>1759-9660</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpdkU9LHTEUxYfSQq3tpvtCwE0pTE1mOvmzfGjVguhCu-hqyCQ370VnkudNBvGz-eXM-EShq1xyf-dwuKeqvjL6k9FWHdpWP1BWxs27ao-JTtWKC_X-deb0Y_UppRtKuWo526ser2Z02gCZovXOG519DCQ6okkyCBDqLfqQwRIYwWSMFsi9z5uyd2O8B6xHfwsk6BBTxtnkGYHkSJwecHGDAq5nHXwAcnyxqged3rzMBqYCjWTwMUFIERephQw4LYK8KfKQCxIMILE4r8m2SNYlZfDD5-qD02OCLy_vfvX35Pf10Vl9fnn652h1XhsmZa6FcVK1opGdgw6ksACDappuaMqX5VoNouOi3KNrpLWKSumEan5Ro1pnwNh2v_q-891ivJsh5X7yycA46gBxTn0jueKs41QW9OA_9CbOGEq6QoluycF4oX7sKIMxJQTXlyNPGh96Rvulx_64Xf177vGswN92MCbzyr313D4BalieMg</recordid><startdate>20231012</startdate><enddate>20231012</enddate><creator>Al-Qargholi, Basim</creator><creator>Al-dolaimy, F</creator><creator>Altalbawy, Farag M. A</creator><creator>Kadhim, Abed J</creator><creator>Alsaalamy, Ali Hashiem</creator><creator>Suliman, Muath</creator><creator>Abbas, Ahmed hussien R</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0004-8854-6151</orcidid></search><sort><creationdate>20231012</creationdate><title>Surface modification of a screen-printed electrode with a flower-like nanostructure to fabricate a guanine DNA-based electrochemical biosensor to determine the anticancer drug pemigatinib</title><author>Al-Qargholi, Basim ; Al-dolaimy, F ; Altalbawy, Farag M. A ; Kadhim, Abed J ; Alsaalamy, Ali Hashiem ; Suliman, Muath ; Abbas, Ahmed hussien R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c188t-7cf8937285fe5e87deeb9225b2285d6a9b7567693528dd9088f79240c93fcecd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antitumor agents</topic><topic>Biosensors</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Electrons</topic><topic>Europium</topic><topic>Grooves</topic><topic>Guanine</topic><topic>Manganese dioxide</topic><topic>Molecular docking</topic><topic>Nanostructure</topic><topic>Oxidation</topic><topic>Plasma proteins</topic><topic>Polyanilines</topic><topic>Three dimensional flow</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Al-Qargholi, Basim</creatorcontrib><creatorcontrib>Al-dolaimy, F</creatorcontrib><creatorcontrib>Altalbawy, Farag M. A</creatorcontrib><creatorcontrib>Kadhim, Abed J</creatorcontrib><creatorcontrib>Alsaalamy, Ali Hashiem</creatorcontrib><creatorcontrib>Suliman, Muath</creatorcontrib><creatorcontrib>Abbas, Ahmed hussien R</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al-Qargholi, Basim</au><au>Al-dolaimy, F</au><au>Altalbawy, Farag M. A</au><au>Kadhim, Abed J</au><au>Alsaalamy, Ali Hashiem</au><au>Suliman, Muath</au><au>Abbas, Ahmed hussien R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface modification of a screen-printed electrode with a flower-like nanostructure to fabricate a guanine DNA-based electrochemical biosensor to determine the anticancer drug pemigatinib</atitle><jtitle>Analytical methods</jtitle><date>2023-10-12</date><risdate>2023</risdate><volume>15</volume><issue>39</issue><spage>5146</spage><epage>5156</epage><pages>5146-5156</pages><issn>1759-9660</issn><eissn>1759-9679</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The present study developed a DNA biosensor to determine pemigatinib for the first time. Three-dimensional carnation flower-like Eu
3+
:β-MnO
2
nanostructures (3D CF-L Eu
3+
:β-MnO
2
NSs) and a screen-printed electrode (SPE) modified with polyaniline (PA) were employed. The double-stranded DNA was also immobilized completely on the PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE. Then, electrochemical techniques were used for characterizing the modified electrode. After that, the interaction between pemigatinib and DNA was shown by a reduction in the oxidation current of guanine using differential pulse voltammetry (DPV). According to the analysis, the dynamic range of pemigatinib was between 0.001 and 180.0 μM, indicating the new electrode has a low limit of detection (LOD = 0.23 nM) for pemigatinib. Afterwards, pemigatinib in real samples was measured using the PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE loaded with ds-DNA. The proposed DNA biosensor showed good selectivity toward pemigatinib in the presence of other interference analytes, such as other ions, structurally related pharmaceuticals, and plasma proteins. In addition, the interaction site of pemigatinib with DNA was predicted by molecular docking, which showed the interaction of pemigatinib with the guanine bases of DNA through a groove binding mode. Finally, we employed the
t
-test to verify the capability of the ds-DNA/PA/3D CF-L Eu
3+
:β-MnO
2
NSs/SPE for analyzing pemigatinib in real samples.
The present study developed a DNA biosensor to determine pemigatinib for the first time.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d3ay01103h</doi><tpages>11</tpages><orcidid>https://orcid.org/0009-0004-8854-6151</orcidid></addata></record> |
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| ispartof | Analytical methods, 2023-10, Vol.15 (39), p.5146-5156 |
| issn | 1759-9660 1759-9679 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2869615608 |
| source | Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list) |
| subjects | Antitumor agents Biosensors Deoxyribonucleic acid DNA Electrochemistry Electrodes Electrons Europium Grooves Guanine Manganese dioxide Molecular docking Nanostructure Oxidation Plasma proteins Polyanilines Three dimensional flow |
| title | Surface modification of a screen-printed electrode with a flower-like nanostructure to fabricate a guanine DNA-based electrochemical biosensor to determine the anticancer drug pemigatinib |
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