Laboratory and clinical practices in antinuclear antibody detection and related antigens: recommendations from a Spanish multicentre survey

Antinuclear antibodies (ANA) are the most widely used immunological test for the diagnosis of autoimmune diseases. Despite the recommendations of experts, there is some variability in performing and interpreting this test in routine practice. In this context, the Spanish Group on Autoimmune Diseases...

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Published in:Immunologic research 2023-10, Vol.71 (5), p.749-759
Main Authors: Martinez-Martinez, Laura, Irure-Ventura, Juan, Jurado, Aurora, Roy, Garbiñe, Montes, Marco Antonio, Barrios, Yvelise, Rodríguez-Bayona, Beatriz, Vergara, Esther, Prada, Álvaro, Rojo, Ricardo, Vargas, María Luisa
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Language:English
Subjects:
Allergology
Anti-DNA antibodies
Antibodies
Antigens
Antinuclear antibodies
Autoimmune diseases
Autoimmunity
Biomedical and Life Sciences
Biomedicine
Immunofluorescence
Immunology
Internal Medicine
Laboratories
Medicine/Public Health
Original Article
Polls & surveys
Standardization
Surveys
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cited_by cdi_FETCH-LOGICAL-c375t-dd53d5487b46427809bc209dba2d1d6f10963c32ad935648e3867f366e3b869b3
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container_title Immunologic research
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creator Martinez-Martinez, Laura
Irure-Ventura, Juan
Jurado, Aurora
Roy, Garbiñe
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Vergara, Esther
Prada, Álvaro
Rojo, Ricardo
Vargas, María Luisa
description Antinuclear antibodies (ANA) are the most widely used immunological test for the diagnosis of autoimmune diseases. Despite the recommendations of experts, there is some variability in performing and interpreting this test in routine practice. In this context, the Spanish Group on Autoimmune Diseases (GEAI) of the Spanish Society of Immunology (SEI) conducted a national survey of 50 autoimmunity laboratories. Here we report the survey results on ANA testing, detection of related antigens, and our recommendations. The survey showed that most of the participating laboratories use a similar approach for most key practices: 84% perform ANA by indirect immunofluorescence (IIF) on HEp-2 cells as the screening methodology while the other laboratories use IIF to confirm positive screens; 90% report ANA test results as either negative or positive with titer and pattern; 86% indicated that the ANA pattern conditioned follow-up testing for specific antigen-related antibodies; and 70% confirm positive anti-dsDNA. However, testing practices were highly heterogeneous for certain items, such as sera dilutions and the minimum time period for repeating ANA and related antigen determinations. Overall, this survey shows that most autoimmune laboratories in Spain use a similar approach but that further standardization of testing and reporting protocols is needed.
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subjects Allergology
Anti-DNA antibodies
Antibodies
Antigens
Antinuclear antibodies
Autoimmune diseases
Autoimmunity
Biomedical and Life Sciences
Biomedicine
Immunofluorescence
Immunology
Internal Medicine
Laboratories
Medicine/Public Health
Original Article
Polls & surveys
Standardization
Surveys
title Laboratory and clinical practices in antinuclear antibody detection and related antigens: recommendations from a Spanish multicentre survey
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