Improve L-isoleucine production in Corynebacterium glutamicum WM001 by destructing the biosynthesis of trehalose dicorynomycolate

Trehalose dicorynomycolates are structurally important constituents of the cell envelope in Corynebacterium glutamicum. The genes treS, treY, otsA, mytA and mytB are necessary for the biosynthesis of trehalose dicorynomycolates. In this study, the effect of biosynthesis of trehalose dicorynomycolate...

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Published in:Microbiological research 2023-07, Vol.272, p.127390-127390, Article 127390
Main Authors: Li, Hedan, Xu, Daqing, Zhang, Dezhi, Tan, Xin, Huang, Danyang, Ma, Wenjian, Zhao, Guihong, Li, Ying, Liu, Ziwei, Wang, Yang, Hu, Xiaoqing, Wang, Xiaoyuan
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Language:English
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Cord Factors
Corynebacterium glutamicum
Corynebacterium glutamicum - genetics
Isoleucine
L-isoleucine production
Trehalose
Trehalose biosynthesis
Trehalose dicorynomycolates
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container_title Microbiological research
container_volume 272
creator Li, Hedan
Xu, Daqing
Zhang, Dezhi
Tan, Xin
Huang, Danyang
Ma, Wenjian
Zhao, Guihong
Li, Ying
Liu, Ziwei
Wang, Yang
Hu, Xiaoqing
Wang, Xiaoyuan
description Trehalose dicorynomycolates are structurally important constituents of the cell envelope in Corynebacterium glutamicum. The genes treS, treY, otsA, mytA and mytB are necessary for the biosynthesis of trehalose dicorynomycolates. In this study, the effect of biosynthesis of trehalose dicorynomycolates on L-isoleucine production in C. glutamicum has been investigated by deleting the genes treS, treY, otsA, mytA, and mytB in the L-isoleucine producing C. glutamicum WM001. L-isoleucine production was slightly improved in the mutants ΔtreY, ΔotsA, and ΔtreYA, and not improved in the single deletion mutant ΔtreS , but significantly improved in the triple deletion mutant ΔtreSYA. Deletion of mytA or mytB in ΔtreSYA could further improve L-isoleucine production. However, deletion of both mytA and mytB in ΔtreSYA significantly decreased L-isoleucine production. The final L-isoleucine producing C. glutamicum WL001 was constructed by deletion of treS, treY, otsA, and mytB, insertion of lrp, and replacement of the native promoter of ilvA with the L-isoleucine sensitive promoter PbrnFE7. WL001 grew worse than the control WM001, but produced 36.1% more L-isoleucine after 72 h shake flask cultivation than WM001.
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subjects Cord Factors
Corynebacterium glutamicum
Corynebacterium glutamicum - genetics
Isoleucine
L-isoleucine production
Trehalose
Trehalose biosynthesis
Trehalose dicorynomycolates
title Improve L-isoleucine production in Corynebacterium glutamicum WM001 by destructing the biosynthesis of trehalose dicorynomycolate
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