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A fibrinogen-related Lectin from Echinometra lucunter represents a new FReP family in Echinodermata phylum
Fibrinogen-related proteins (FREPs) have been identified in several animals. They are involved in the body's defense, acting as mediators of phagocytosis. Ficolins and intelectins are some of the most studied Fibrinogen-related Domain (FReD)-containing lectins. In this work, we have isolated a...
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| Published in: | Fish & shellfish immunology 2022-12, Vol.131, p.150-159 |
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| container_title | Fish & shellfish immunology |
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| creator | Duarte, Philippe Lima Andrade, Francisco Regivânio Nascimento Sousa, Andressa Rocha de Oliveira Andrade, Alexandre Lopes de Vasconcelos, Mayron Alves Teixeira, Edson Holanda Nagano, Celso Shiniti Sampaio, Alexandre Holanda Carneiro, Rômulo Farias |
| description | Fibrinogen-related proteins (FREPs) have been identified in several animals. They are involved in the body's defense, acting as mediators of phagocytosis. Ficolins and intelectins are some of the most studied Fibrinogen-related Domain (FReD)-containing lectins. In this work, we have isolated a singular FReD-containing lectin, which cannot be classified as ficolin or intelectin. ELL (Echinometra lucunter lectin) was isolated from coelomic plasma by affinity chromatography on xanthan gum. Primary structure was determined by tandem mass spectrometry. Moreover, antimicrobial activity of ELL was evaluated against planktonic cells and biofilm of Escherichia coli, Staphylococcus aureus and S. epidermidis. ELL showed hemagglutinating activity in Ca2+ presence, which was inhibited by glycoprotein mucin and thyroglobulin. Complete amino acid sequence consisted of 229 residues, including a FReD in the N-terminal. Searches for similarity found that ELL was very close to putative proteins from Strongylocentrotus purpuratus. ELL showed moderate similarity with uncharacterized sea stars proteins and protochordate intelectins. ELL was able to inhibit the planktonic growth of the Gram-positive bacteria and significantly reduce the biofilm formation of all bacteria tested. In conclusion, we identified a new type of FReP-containing lectin with some structural and functional conservation towards intelectins.
•A Fibrinogen-related protein (FREP) was isolated from sea urchin Echinometra lcuunter.•ELL she is the first member of a new family of FREPs found in echinoderms.•ELL seems to be involved in the defense of the E. lucunter.•ELL was able to inhibit the planktonic growth of the Gram-positive bacteria. |
| doi_str_mv | 10.1016/j.fsi.2022.10.007 |
| format | article |
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•A Fibrinogen-related protein (FREP) was isolated from sea urchin Echinometra lcuunter.•ELL she is the first member of a new family of FREPs found in echinoderms.•ELL seems to be involved in the defense of the E. lucunter.•ELL was able to inhibit the planktonic growth of the Gram-positive bacteria.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2022.10.007</identifier><identifier>PMID: 36216229</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antibacterial activity ; Echinodermata - metabolism ; Escherichia coli ; Fibrinogen - genetics ; Fibrinogen-related protein ; FReD ; Intelectin ; Lectin ; Lectins - genetics ; Lectins - metabolism ; Lectins - pharmacology ; Sea urchin ; Sequence Alignment ; Staphylococcus aureus - metabolism</subject><ispartof>Fish & shellfish immunology, 2022-12, Vol.131, p.150-159</ispartof><rights>2022 Elsevier Ltd</rights><rights>Copyright © 2022 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c305t-471c6eff0a51a05b780e6bb9620d26211dd0e285fd94ef27bfc00c78bc6cb07d3</cites><orcidid>0000-0003-0024-2512</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36216229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duarte, Philippe Lima</creatorcontrib><creatorcontrib>Andrade, Francisco Regivânio Nascimento</creatorcontrib><creatorcontrib>Sousa, Andressa Rocha de Oliveira</creatorcontrib><creatorcontrib>Andrade, Alexandre Lopes</creatorcontrib><creatorcontrib>de Vasconcelos, Mayron Alves</creatorcontrib><creatorcontrib>Teixeira, Edson Holanda</creatorcontrib><creatorcontrib>Nagano, Celso Shiniti</creatorcontrib><creatorcontrib>Sampaio, Alexandre Holanda</creatorcontrib><creatorcontrib>Carneiro, Rômulo Farias</creatorcontrib><title>A fibrinogen-related Lectin from Echinometra lucunter represents a new FReP family in Echinodermata phylum</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Fibrinogen-related proteins (FREPs) have been identified in several animals. They are involved in the body's defense, acting as mediators of phagocytosis. Ficolins and intelectins are some of the most studied Fibrinogen-related Domain (FReD)-containing lectins. In this work, we have isolated a singular FReD-containing lectin, which cannot be classified as ficolin or intelectin. ELL (Echinometra lucunter lectin) was isolated from coelomic plasma by affinity chromatography on xanthan gum. Primary structure was determined by tandem mass spectrometry. Moreover, antimicrobial activity of ELL was evaluated against planktonic cells and biofilm of Escherichia coli, Staphylococcus aureus and S. epidermidis. ELL showed hemagglutinating activity in Ca2+ presence, which was inhibited by glycoprotein mucin and thyroglobulin. Complete amino acid sequence consisted of 229 residues, including a FReD in the N-terminal. Searches for similarity found that ELL was very close to putative proteins from Strongylocentrotus purpuratus. ELL showed moderate similarity with uncharacterized sea stars proteins and protochordate intelectins. ELL was able to inhibit the planktonic growth of the Gram-positive bacteria and significantly reduce the biofilm formation of all bacteria tested. In conclusion, we identified a new type of FReP-containing lectin with some structural and functional conservation towards intelectins.
•A Fibrinogen-related protein (FREP) was isolated from sea urchin Echinometra lcuunter.•ELL she is the first member of a new family of FREPs found in echinoderms.•ELL seems to be involved in the defense of the E. lucunter.•ELL was able to inhibit the planktonic growth of the Gram-positive bacteria.</description><subject>Animals</subject><subject>Antibacterial activity</subject><subject>Echinodermata - metabolism</subject><subject>Escherichia coli</subject><subject>Fibrinogen - genetics</subject><subject>Fibrinogen-related protein</subject><subject>FReD</subject><subject>Intelectin</subject><subject>Lectin</subject><subject>Lectins - genetics</subject><subject>Lectins - metabolism</subject><subject>Lectins - pharmacology</subject><subject>Sea urchin</subject><subject>Sequence Alignment</subject><subject>Staphylococcus aureus - metabolism</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EouXxAWyQl2xSxk7iJGJVVRSQKoEQrC3HHoOrPIqdgPr3uCqwZDUznnuvxoeQCwYzBkxcr2c2uBkHzuM8AygOyJRBlSdVlRWHuz6HJBNZOSEnIawBQKQCjskkFZwJzqspWc-pdbV3Xf-GXeKxUQMaukI9uI5a37f0Vr_HbYuDV7QZ9dgN6KnHjceA3RCooh1-0eUzPlGrWtdsaXTuTQZ9qwZFN-_bZmzPyJFVTcDzn3pKXpe3L4v7ZPV497CYrxKdQj4kWcG0QGtB5UxBXhcloKjrSnAwPN7NjAHkZW5NlaHlRW01gC7KWgtdQ2HSU3K1z934_mPEMMjWBY1NozrsxyB5wdOSpSXwKGV7qfZ9CB6t3HjXKr-VDOQOsVzLiFjuEO-eIuLoufyJH-sWzZ_jl2kU3OwFGD_56dDLoB12Go3zkas0vfsn_hsC1o1q</recordid><startdate>202212</startdate><enddate>202212</enddate><creator>Duarte, Philippe Lima</creator><creator>Andrade, Francisco Regivânio Nascimento</creator><creator>Sousa, Andressa Rocha de Oliveira</creator><creator>Andrade, Alexandre Lopes</creator><creator>de Vasconcelos, Mayron Alves</creator><creator>Teixeira, Edson Holanda</creator><creator>Nagano, Celso Shiniti</creator><creator>Sampaio, Alexandre Holanda</creator><creator>Carneiro, Rômulo Farias</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0024-2512</orcidid></search><sort><creationdate>202212</creationdate><title>A fibrinogen-related Lectin from Echinometra lucunter represents a new FReP family in Echinodermata phylum</title><author>Duarte, Philippe Lima ; Andrade, Francisco Regivânio Nascimento ; Sousa, Andressa Rocha de Oliveira ; Andrade, Alexandre Lopes ; de Vasconcelos, Mayron Alves ; Teixeira, Edson Holanda ; Nagano, Celso Shiniti ; Sampaio, Alexandre Holanda ; Carneiro, Rômulo Farias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-471c6eff0a51a05b780e6bb9620d26211dd0e285fd94ef27bfc00c78bc6cb07d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Antibacterial activity</topic><topic>Echinodermata - metabolism</topic><topic>Escherichia coli</topic><topic>Fibrinogen - genetics</topic><topic>Fibrinogen-related protein</topic><topic>FReD</topic><topic>Intelectin</topic><topic>Lectin</topic><topic>Lectins - genetics</topic><topic>Lectins - metabolism</topic><topic>Lectins - pharmacology</topic><topic>Sea urchin</topic><topic>Sequence Alignment</topic><topic>Staphylococcus aureus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duarte, Philippe Lima</creatorcontrib><creatorcontrib>Andrade, Francisco Regivânio Nascimento</creatorcontrib><creatorcontrib>Sousa, Andressa Rocha de Oliveira</creatorcontrib><creatorcontrib>Andrade, Alexandre Lopes</creatorcontrib><creatorcontrib>de Vasconcelos, Mayron Alves</creatorcontrib><creatorcontrib>Teixeira, Edson Holanda</creatorcontrib><creatorcontrib>Nagano, Celso Shiniti</creatorcontrib><creatorcontrib>Sampaio, Alexandre Holanda</creatorcontrib><creatorcontrib>Carneiro, Rômulo Farias</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duarte, Philippe Lima</au><au>Andrade, Francisco Regivânio Nascimento</au><au>Sousa, Andressa Rocha de Oliveira</au><au>Andrade, Alexandre Lopes</au><au>de Vasconcelos, Mayron Alves</au><au>Teixeira, Edson Holanda</au><au>Nagano, Celso Shiniti</au><au>Sampaio, Alexandre Holanda</au><au>Carneiro, Rômulo Farias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A fibrinogen-related Lectin from Echinometra lucunter represents a new FReP family in Echinodermata phylum</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2022-12</date><risdate>2022</risdate><volume>131</volume><spage>150</spage><epage>159</epage><pages>150-159</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Fibrinogen-related proteins (FREPs) have been identified in several animals. They are involved in the body's defense, acting as mediators of phagocytosis. Ficolins and intelectins are some of the most studied Fibrinogen-related Domain (FReD)-containing lectins. In this work, we have isolated a singular FReD-containing lectin, which cannot be classified as ficolin or intelectin. ELL (Echinometra lucunter lectin) was isolated from coelomic plasma by affinity chromatography on xanthan gum. Primary structure was determined by tandem mass spectrometry. Moreover, antimicrobial activity of ELL was evaluated against planktonic cells and biofilm of Escherichia coli, Staphylococcus aureus and S. epidermidis. ELL showed hemagglutinating activity in Ca2+ presence, which was inhibited by glycoprotein mucin and thyroglobulin. Complete amino acid sequence consisted of 229 residues, including a FReD in the N-terminal. Searches for similarity found that ELL was very close to putative proteins from Strongylocentrotus purpuratus. ELL showed moderate similarity with uncharacterized sea stars proteins and protochordate intelectins. ELL was able to inhibit the planktonic growth of the Gram-positive bacteria and significantly reduce the biofilm formation of all bacteria tested. In conclusion, we identified a new type of FReP-containing lectin with some structural and functional conservation towards intelectins.
•A Fibrinogen-related protein (FREP) was isolated from sea urchin Echinometra lcuunter.•ELL she is the first member of a new family of FREPs found in echinoderms.•ELL seems to be involved in the defense of the E. lucunter.•ELL was able to inhibit the planktonic growth of the Gram-positive bacteria.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>36216229</pmid><doi>10.1016/j.fsi.2022.10.007</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0024-2512</orcidid></addata></record> |
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| ispartof | Fish & shellfish immunology, 2022-12, Vol.131, p.150-159 |
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| source | ScienceDirect Journals |
| subjects | Animals Antibacterial activity Echinodermata - metabolism Escherichia coli Fibrinogen - genetics Fibrinogen-related protein FReD Intelectin Lectin Lectins - genetics Lectins - metabolism Lectins - pharmacology Sea urchin Sequence Alignment Staphylococcus aureus - metabolism |
| title | A fibrinogen-related Lectin from Echinometra lucunter represents a new FReP family in Echinodermata phylum |
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